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Summary of Contents for tecan GENios
- Page 1 Operating Manual for GENios, GENios FL, and GENios Plus Document Part No. I 112 904 June 2002 Document revision: 1.1 Software level: 4.4x...
- Page 2 Theale Court 11-13 High Street Theale UK-Reading RG7 5AH United Kingdom Tel.: +44 11 89 300 300 Fax: +44 11 89 305 671 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002...
- Page 3 Install the instrument • Operate the instrument • Insert and define filter slides • Perform quality control tests • Clean and maintain the instrument June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1...
- Page 4 WARNING INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT FOLLOWED. Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002...
- Page 5 A-5082 Grödig City / State / Zip code: -------------------------------------------- AUSTRIA Country: -------------------------------------------- --------------------------------------- Phone: --------------------------------------- Fax: Applications / --------------------------------------- Field of use: --------------------------------------- June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1...
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Page 6: Table Of Contents
6. Interface Hardware Specifications............... 6-1 Pin Designation ................6-1 RS-232-C Interface Lines .............. 6-2 Synchronization and Data Format ..........6-3 Signal Levels.................. 6-3 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... - Page 7 Results from Plasmid and Blood DNA Sample........ 9-4 9.3.4 DNA Purity at 260/280 nm ............... 9-5 Conclusion ..................9-5 Acknowledgements ................. 9-5 Index June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1...
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Page 9: Safety
4. Never open the instrument while it is plugged into a power source. 5. Never force a microplate or filter slide into the instrument. 6. The GENios, GENios FL, and GENios Plus are intended for laboratory research use only. Observe proper laboratory safety precautions, such as wearing protective clothing and using approved laboratory safety procedures. -
Page 11: General Description
The filters are arranged in filter slides; four excitation filters and four emission filters respectively. The instrument has no on-board software and is exclusively controlled via an external computer using Windows format. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 12: The Genios, Genios Fl, And Genios Plus
Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 13: Installation
Check the instrument accessories against the packing list. Save packing materials and transport lock for further transportation purposes. Define and insert the filters June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 14: Removal Of The Transport Lock
(See diagram below). 2 screws Remove the transport lock. Note: The transport lock should be saved for further transportation purposes. Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 15: Power Requirements
Fuse = T 2A / 250 (slow blow) WARNING RISK OF FIRE! REPLACE ONLY WITH THE SAME TYPE AND RATING OF FUSE. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 16: Switching The Instrument On
Consequently the safety of the instrument can not be guaranteed. To operate the software, refer to the XFLUOR manual. Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 17: Inserting And Defining Filter Slides
(FWHM) is usually larger than 10 nm. This could cause a bright value error or low OD values when measuring dyes with narrow peaks. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 18: Organization Of Filters In Filter Slides
EM as B 126102 coded as requested * * Without request it is coded as "B". The barcodes are labeled as follows: Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 19: Inserting The Filter Slides
If the required filter is fitted in the filter slide, the instrument then moves the filter slide so that the required filter is in the light beam. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 20: How Do I Install The Custom Filter
Do not use the two bar codes currently used for the standard excitation (A) emission (A), and absorbance (D) filter slides for a combination of customized filters. Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... - Page 21 The EX/ABS and EM filter slide can have the same coding. The filter slide is now ready to be placed into the appropriate filter slide opening and be recognized by the software. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1...
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Page 22: Defining The Filter
Exit RdrOLE by clicking Exit in the File menu and restart XFLUOR. This completes the installation procedure. Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 23: Instrument Features
Via scanning software Temperature control From ambient +5°C up to 42°C Plate shaking Orbital or linear shaking - both offer 3 speeds June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... - Page 24 Relative humidity Operation 90% non-condensing Overvoltage category Pollution degree Usage Commercial Noise level < 70 dBA Method of disposal Electronic waste (infectious waste) Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002...
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Page 25: Fluorescence
(on the fly) 4 - 255 Plate carrier stops max. precision Dependent on the at the meas. point flash parameter setting June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 26: Absorbance
1 - 5000 ms 0 - 55,000 RLU Maximum Dynamic automatic 0 - 55,000,000 RLU Range (1 - 1000 ms) (using XF LUOR Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 27: Specifications (Genios Fl)
The instrument allows for kinetic measurements Reading may be restricted to one part of the microplate. Up to four-measurement parameter sets can be queued using XF LUOR June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... - Page 28 Relative humidity Operation 90% non-condensing Overvoltage category Pollution degree Usage Commercial Noise level < 70 dBA Method of disposal Electronic waste (infectious waste) Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002...
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Page 29: Fluorescence
(on the fly) 4 - 255 Plate carrier stops max. precision Dependent on the at the meas. point flash parameter setting June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 30: Specifications (Genios Plus)
Via scanning software Temperature control From ambient +5°C up to 42°C Plate shaking Orbital or linear shaking - both offer 3 speeds Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... - Page 31 Relative humidity Operation 90% non-condensing Overvoltage category Pollution degree Usage Commercial Noise level < 70 dBA Method of disposal Electronic waste (infectious waste) June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1...
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Page 32: Fluorescence
(on the fly) 4 - 255 Plate carrier stops max. precision Dependent on the at the meas. point flash parameter setting 5-10 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 33: Absorbance
1 - 5000 ms 0 - 55,000 RLU Maximum Dynamic Automatic 0 - 55,000,000 RLU Range (1 - 1000 ms) (using XF LUOR June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 5-11... -
Page 35: Interface
All connected devices must be approved and listed as per EN 60950, UL 1950 or CSA C22.2 No. 950 for Data Processing Devices. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 36: Rs-232-C Interface Lines
DSR connected to DCD and DTR Use the computer handbook to find the correct pin connections. Instrument Computer 25 pin Instrument Computer 9 pin Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 37: Synchronization And Data Format
DATA SIGNALS CONTROL SIGNALS +3 to +12 SPACE (Logic 0) -3 to -12 MARK (Logic 1) +3 to -3 UNDEFINED RANGE UNDEFINED RANGE June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 39: Quality Control
All other wells are blank (B), for example: 200 µl 0.01 M NaOH solution without fluorescein • Use 100 flashes and optimal gain function. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 40: Linearity Test
1 to 11. Compute the coefficient of correlation using the blanked average RFUs and the corresponding concentrations. The coefficient of correlation is expected to be ≥ 0.995. Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 41: Precision Test
CT = (MVN – MVB ) / Average RFU (E2, E5, E8, E11). CT is expected to be ≤ 0.0001. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 42: Glow Type Luminescence
Multiply with a factor of 2 to achieve statistical reliability. The sensitivity is expected to be <0.4 10 DEA activity units/well Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 43: Cross-Talk Test
If MVN is higher than MVB +3 SD the cross talk CT (Cross Talk) calculates according to: CT = (MVN – MVB ) / RLU reading CT is expected to be ≤ 0.0001 June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 45: Cleaning & Maintenance
THE INSTRUMENT BEFORE IT IS PREPARED FOR SHIPMENT. IF A MICROPLATE IS LEFT IN THE INSTRUMENT, FLUORESCENT SOLUTIONS MAY SPILL ONTO THE OPTICAL PARTS AND DAMAGE THE INSTRUMENT. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 46: Replacing The Fuse
Replace the fuse holder. • Reconnect the mains cable. • Turn the instrument on. If the fuse continues to blow, call for service. Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 47: Maintenance
Clean the filters using an optical cleaning solution (Lens tissue recommended). 8.4.4 Yearly Maintenance Check the belts tension (between 50 an 60 Hz, tension meter necessary). Perform a FlouCheck test. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 48: Instrument Disinfection
Please note that the disinfectant can influence the performance of your instrument if it applied inside the instrument. Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 49: Disinfection Procedure
See 8.5 for an example of the disinfection certificate that must be completed before the instrument is returned to the distributor for servicing. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 50: Disinfection Certificate
Name:........................Firm: ........................Address: ......................................................................... Country:........................ Signature:......................Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 51: Application Note For Dna Quantification
Molecular Probes are also known for the quantitation of nucleic acids - 0.5 ng/ml DNA in solution is the detectable concentration. June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 52: Experimental Setup
). The solutions were mixed by shaking for 5 sec prior to the measurement. A dilution series of λ DNA was used as calibration standard. Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 53: Typical Results
Molecular Probes) measured with absorbance at 260 nm Figure 2 Dilution series of λ-DNA measured with PicoGreen fluorescence at 485 nm (excitation) and 535 nm (emission ). June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 54: Sensitivities In Comparison
Table 2 Comparison of the stock concentrations (referring to the calibration curves above) in µg/ml of the samples measured with fluorescence (485/535) and absorbance (260). Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 June 2002... -
Page 55: Dna Purity At 260/280 Nm
We would like to thank Robert Liebhart for carrying out the practical experiments at ETH Zürich, Inst. for Plant Sciences (Phytopathology), Universitätsstrasse 2, CH-8092 Zürich (Laboratory Dr. Cesare Gessler). June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1... -
Page 57: Index
Linearity ............7-2 GENIOS............2-2 Precision ............. 7-2 Glow Type Luminescence ..... 5-5, 5-17, 7-4 Sensitivity..........7-1, 7-4 Hardware Specifications......... 6-1 June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1 Index...
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