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Summary of Contents for tecan Resolvex A200
- Page 1 Operating Manual for Resolvex A200 A200 Resolvex ™ Operating Manual 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 1/73...
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Page 2: Table Of Contents
Software Installation __________________________________________________17 3.11 Gas Fittings Connections ______________________________________________17 3.12 Functional Tests _____________________________________________________18 Product Warranty ___________________________________________________18 Before Starting the Machine ___________________________________________18 Solvent Line Fittings _________________________________________________19 SPE Rack Configurations and Use _____________________________________20 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 2/73... - Page 3 Save Button _________________________________________________________48 8.12.3 Delete Button ________________________________________________________48 8.12.4 Okay Button _________________________________________________________48 8.12.5 Low / High Selection __________________________________________________48 8.12.6 Add and Delete Point Buttons ___________________________________________49 8.12.7 Selecting and Deselecting Points ________________________________________49 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 3/73...
- Page 4 Drain Tubes _________________________________________________________59 11.3.8 Column Seal Replacement _____________________________________________60 Default Passwords __________________________________________________61 Resolvex™ A200 24 Configuration _____________________________________62 Resolvex™ A200 “Omics” Configuration ________________________________63 File Security Features ________________________________________________63 User Control Features ________________________________________________63 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 4/73...
- Page 5 File Management _____________________________________________________68 16.4.2 File Management Form ________________________________________________69 Cross-References ___________________________________________________69 17.1 References _________________________________________________________69 Abbreviations, Trademarks and Symbols ________________________________70 18.1 Abbreviations ________________________________________________________70 18.2 Trademarks _________________________________________________________71 18.3 Tecan Customer Support ______________________________________________72 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 5/73...
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Page 6: Safety
Intended Use The Tecan Resolvex A200 device will be used to perform solid phase extraction of analytes from various liquid sample types as a stand-alone module or coupled to a larger system for sample preparation. The device is intended to be used in a professional laboratory setting to perform various solid phase extraction procedures using positive pressure application to filter/extraction plates to prepare samples for downstream analysis (e.g. -
Page 7: Applicable Symbols
Indicates the temperature limits to which the unit can be safely exposed Indicates the date after which the product is not to be used WEEE Symbol China RoHS label CE Mark CSA Label UKCA Mark 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 7/73... -
Page 8: Warning Symbols
Follow laboratory procedures for biohazardous waste disposal according to national and local regulations. This chapter provides instructions for how to lawfully dispose of waste material accumulating in connection with the Tecan Resolvex A200 device. CAUTION OBSERVE ALL FEDERAL, STATE AND LOCAL ENVIRONMENTAL REGULATIONS 1.6.2... - Page 9 Disposal of Operating Material WARNING BIOLOGICAL HAZARDS CAN BE ASSOCIATED WITH THE WASTE MATERIAL (MICROPLATE) OF THE PROCESS RUN ON THE TECAN Resolvex A200 DEVICE TREAT THE USED MICROPLATE, OTHER DISPOSABLES, AND ALL SUBSTANCES USED IN ACCORDANCE WITH GOOD LABORATORY PRACTICE GUIDELINES.
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Page 10: Laboratory Practices
Suitable education or training in the handling of chemicals is highly recommended. Product Overview The Tecan Resolvex CONTROL Software is a MS Windows® application that is used to control the Tecan Resolvex A200 device which has been designed to facilitate the automated processing... -
Page 11: Major Components
Critical to the machines ability to accurately dispense is the tubing that runs between the four port solvent manifold and the eight port dispense head. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 11/73... -
Page 12: Dispense Head Pump Coupling
2.1.7 Dispense head pump coupling Looking at the eight port dispense head from the front of the Resolvex A200, the left four ports are coupled to the bottom pump and the right four ports are coupled to the top pump. -
Page 13: Gas Requirements
Tecan sales representative to get an adequately rated replacement part. Gas Requirements The Tecan Resolvex A200 operates using compressed air or nitrogen both to seal the columns and to displace liquid and must also be within 60” (1524mm) of the installation site. -
Page 14: Environmental Conditions
Lifting Lift the Resolvex A200 out of the carton and place it in the selected location. Take care when lifting the unit and ensure that it is held on both sides. The unit is heavy (greater than 18kg - 40 lbs, see above) and may require two people to lift it. -
Page 15: Supplied Parts
Operating Manual for Resolvex A200 3.8.2 Supplied Parts The Resolvex A200-96 and Omics are shipped with the 253-5457 Accessory Kit which contains the following items. NOTE: The Omics unit comes with the additional 30189741 KIT UPGRADE PROTEOMICS QTY. Part Number... -
Page 16: Associated Parts
DOC CHECKLIST REMOTE INSTALLATION A200 30139045 DOC A200 DECLARATION OF CONFORMITY 3.8.3 Associated Parts The Resolvex A200 is designed to work with one of the following AC mains power cords that is ordered separately depending on destination country. Part Number Region Plug/Connector Type... -
Page 17: Installing
Operating Manual for Resolvex A200 Installing The Resolvex A200 does not include a filter on the gas supply input. A clean, oil-free gas source must be used to prevent contamination of the pneumatics and the consumables containing samples. 1. Open the crate and remove the accessories boxes. Standard contents are listed above. -
Page 18: Functional Tests
Refer to terms and conditions of contract at time of purchase for warranty information. Spare parts or replacements beyond the warranty period for such components as Resolvex A200 waste bins, syringes, and plumbing items are available. Contact Tecan Customer Service for current options and pricing. -
Page 19: Solvent Line Fittings
It is possible that the fittings on the pump for the solvent lines have loosened during shipping. The flanged fittings can be tightened by hand. The fittings are torque limited and will click when tightened sufficiently. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 19/73... -
Page 20: Spe Rack Configurations And Use
Operating Manual for Resolvex A200 SPE Rack Configurations and Use The Resolvex A200 provides the means to batch process solid phase extraction columns. Depending upon the volume of the eluent, the appropriate collection rack or collection tray can be selected from the ancillary products available with the Resolvex A200. -
Page 21: Resolvex Control Software
Firmware Upgrade The Resolvex A200 control software has the ability to verify if the firmware installed in the control board inside the unit is of the most recent version. If prompted to upgrade, simply follow the on- screen instructions. -
Page 22: Periodic Maintenance Notification
If the user is busy, they can snooze the tasks by pressing “Remind me in one Hour” and the window will close and reappear after 60 minutes. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 22/73... - Page 23 Users may add Tasks by pressing the “Add a New Maintenance Item” button. Then scroll to the blank task and click the boxes to add text, and set the desired frequency. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 23/73...
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Page 24: Advanced Settings
Settings and Setup have individual icons, while the other buttons are opened with the Advanced Icon if the user has access (User Control). They replace the Tecan logo when showing. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 24/73... -
Page 25: Main Dialog
8.6.2 The “Run” button will run a selected method. To select a method, tap on its name. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 25/73... -
Page 26: Light
The 0% button allows for quick resetting of volume for a waste reservoir is emptied. The “X” icon exits the dialogue box, canceling any changes made. The green check icon saves the adjusted solvent volume. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 26/73... -
Page 27: Program
Edit The “Edit” button will open a selected method in the “Method Editor” dialog box for editing or preview. To edit a method, enter the code “change” when prompted. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 27/73... -
Page 28: Add New
The “Analysis” button will analyze the selected method and verify if there is enough solvent to run the selected method as well as report the cost associated for each solvent. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 28/73... -
Page 29: Solvents And Speeds
Warning: Caution must be exercised when selecting speeds. Solvents used have different viscosities that require different aspirate and dispense speeds. Aspirating too fast can lead to bubbles and cavitation. Dispensing too fast can lead to pump stalling, leaks or splashing. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 29/73... -
Page 30: Form Headers
The default should work for most solvents. If there are small bubbles collecting around the dispense tips, the value should be lowered for that solvent. The allowed range is 0 to 100. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 30/73... -
Page 31: Flush Solvent
“Default Solvent DB”. Once a solvent has been selected, click on the green check box button to accept the selected solvent. The “Solvent Name”, “Aspirate Speed” and “Dispense Speed” text boxes will be populated with the selected solvent. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 31/73... - Page 32 The “User Solvent DB” can be updated by clicking on the “User Solvent DB” button. The “Default Solvent DB” can be viewed by clicking on the “Default Solvent DB” button. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 32/73...
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Page 33: Solvent Prime
NOTE: The syringe is not flushed between selecting solvents, so be sure to prime with a buffer solvent if there could be possible issues with large amounts of dissimilar solvents mixing. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 33/73... -
Page 34: Method Editor
Takes method editor out of read only mode and allows the current steps of method to be edited. Can also double click on the table to edit the step selected. 8.10.2.4 Delete Deletes current step. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 34/73... - Page 35 Scroll up or down through the steps of the method. 8.10.2.6 Slide Show Wizard A guide to add new or modify existing slideshows for the Message Only step in Operator Operations. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 35/73...
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Page 36: Dispense Operations
The Dispense Reagent operation dispenses the selected solvent and volume in each of the selected columns over the waste tray. After operation is complete, the system flushes with the system solvent. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 36/73... - Page 37 The specified solvent to dispense into each column for that step. 8.10.3.7 Solvent Info Opens the Solvents and Speeds dialog box. 8.10.3.8 Test Operation (Play) Tests the current step. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 37/73...
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Page 38: Manifold Operations
. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP... - Page 39 Operating Manual for Resolvex A200 8.10.4.1 Column Compression and Decompression Because of the unique manifold design of the Resolvex A200, each column/well is pressurized to the same level even if columns are omitted. 8.10.4.2 Dry The Dry operation pushes air through the columns at maximum flow for a specified amount of time.
- Page 40 High flow is unrestricted, incoming pressure to the Resolvex A200 to be applied in 1% increments. The maximum pressure that can be applied to the columns is 5.5 bar (80 PSI) at 100% flow. If less than 5.5 bar (80 PSI) is available, then the maximum available pressure to the system will be applied.
- Page 41 When not checked the pressure to the columns is completely removed prior to releasing the manifold. 8.10.4.6 Test Operation (Play) Tests the current step. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 41/73...
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Page 42: Operator Operations
(external flash drive: \LauncherPrograms\DispenseSoftware\). Once a message has been selected, it can be customized by touching the edit box on the bottom of the method edit dialog box. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 42/73... - Page 43 The Wait operation is a timer that counts down prior to proceeding to the next step in the method. When inputting a length of time in the parameter field, be sure to follow the numeric value with “Sec” or “Min”. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 43/73...
- Page 44 Note: the appearance of the well plate will vary based on the configurated number of wells. 8.10.5.6 Test Operation (Play) Tests the current step. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 44/73...
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Page 45: Run Program
The “Run Program” dialog box is opened when a method is selected and then the “Run” button is pressed on the main dialog. Before a method is run, the Resolvex A200 software has the ability to verify if the solvents in the method match that of the solvents that are connected to the unit and that there is sufficient solvent to complete a method. -
Page 46: Pause Button
When the run button is pressed prior to starting the run, the “Run Partial” dialog will open and allow selected columns to be processed. When all of the desired columns have been selected, press the “OK” button. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 46/73... -
Page 47: Abort A Method Run
If “Yes” is selected, the method will complete any operations underway, then evacuate any solvent in the syringe pump. The program may prompt for a restart. This will lose any memory of where the last dispense step occurred. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 47/73... -
Page 48: Pressure Profile Editor
High flow provides unrestricted flow to the columns. 100% full scale in low flow is roughly equivalent to 10% full scale in high flow. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 48/73... -
Page 49: Add And Delete Point Buttons
This can be used to determine the length of time for the pressure profile. 8.12.10 Increment Buttons This can be used to determine the length of time for the pressure profile. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 49/73... -
Page 50: 8.12.11 Run Button
The Pressure Display form will be displayed with a black point cursor and infill to indicate the progress along the current pressure profile. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 50/73... -
Page 51: 8.12.12 Pressure Profile Table View
Right click on Pressure Profile in the edit or run screen to open a table view of the profile. There is also a Table View button at the bottom of the window. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 51/73... -
Page 52: Touchpads
For touchpads as in image (b), enter values as desired. This is molded after a standard keyboard. Other non-alphanumeric characters are visible when the shift or caps button is pressed. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 52/73... -
Page 53: Faults And Troubleshooting
How to identify: While solvent is being aspirated into syringe, an air bubble appears at a mid-point in the solvent line. Remedy: Cut out damaged line. Replace line if cutting out damage portion will result in line being too short to submerge in solvent. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 53/73... -
Page 54: A Large Air Bubble Forms At The Top Of The Inner Part Of The Syringe
Verify that the air supply can provide a minimum flow rate greater than the maximum flow consumption of the Resolvex A200. Verify that additional equipment on the same air supply is not starving the Resolvex A200 of required flow. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP... -
Page 55: Pre-Operation Checklist
10. Flush waste tray tubing with distilled or de-ionized water after use and when the unit is not in use to prevent clogging in the tubing. 11. Inspect the waste bottle. The waste bottle should be emptied daily or sooner if ¾ capacity has been reached. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 55/73... -
Page 56: Preventive Maintenance
5. Prime the pump a minimum of 10 cycles with distilled or de-ionized water. 11.1.5 Verification of Light Curtain Operation 1. Initiate a manifold movement within a method and observe that the light curtain remains active during the entire manifold movement. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 56/73... -
Page 57: Semiyearly Maintenance
If either become worn, it is likely that these symptoms will occur: 1. Poor precision and accuracy in dispensing solvents. 2. Large air bubbles accumulating at the top of the syringe (not to be confused with normal cavitation). 3. Leakage. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 57/73... - Page 58 13. Go to the “Replace Syringe Form” and press “Done”. Note: Make sure the plunger lock screw is securely tightened and the plunger button is free to move on the plunger lock screw. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 58/73...
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Page 59: Pump Lead Screw
11.3.4 Cleaning Surface Area 1. The deck of the Resolvex A200 can become dusty or dirty from solvent or sample spills. Periodic cleaning should take place to prevent damage to the surface areas of the platform. -
Page 60: Column Seal Replacement
9. Take the manifold and carefully set it on the standoffs. Fasten the manifold with the two 1/4-20 screws. Be sure to tighten the screws using the 3/16 inch hex key. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 60/73... -
Page 61: Default Passwords
Once passwords have been changed, settings must be saved by pressing the save button on the settings form. Function Password LockPassword cerex EditMethodPassword change DeleteMethodPassword erase EditPressureProfilePassword change EditSolventsPassword change StopRunningProfilePassword stop AlterDryTimePassword Timeflies AlterWaitTimePassword timeflies 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 61/73... -
Page 62: Resolvex™ A200 24 Configuration
The Choose Columns operation can be used to override the columns that were initially selected at the beginning of the method if a specific solvent is not to be used on all the columns in the assay. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 62/73... -
Page 63: Resolvex™ A200 "Omics" Configuration
The User Control Features described in this section are available in Software Revision V 4.0 and higher. These features facilitate Good Laboratory Practices with regard to ensuring that methods are not changed in an uncontrolled manner. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 63/73... -
Page 64: Enable User Control
To enable User Control, open the system config there is a new tab. Click the User Control Check box. Note: There must be a Admin user for this to stay enabled 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 64/73... -
Page 65: Method Types
Favorites - Config Minimize Maximize Exit Edit Add New File Management Solvents Main Program Operate - toggle Profiles Analysis Lock Button -> Advanced Button Settings Setup ** Trap Log 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 65/73... -
Page 66: Add
Opens a windows to add new users. Limited is for the “User” role to limit which of the “Production” methods they are allowed to use. User Enabled allows for a user to be disabled without removing their information. 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 66/73... -
Page 67: Delete
Login button in the lower left click to login, change user, or log out. Right click or click hold on a touch screen to change the password. 16.2.2 Favorites Select Opens the Favorites window 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 67/73... -
Page 68: Favorites Window
Show All available methods Show Only methods selected in the Favorites Window 16.4 Program Mode 16.4.1 File Management Opens the File Management form and replaces the delete method button 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 68/73... -
Page 69: File Management Form
Delete: Deletes methods. Production methods are archived before delete to keep as records. Cross-References 17.1 References Ref. 1: RESOLVEX A200 INSTALLATION QUALIFICATION AND OPERATIONAL QUALIFICATION CHECKLIST, PART NO. 102-5460 Ref. 2: RESOLVEX A200 OPERATOR TRAINING CHECKLIST 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 69/73... -
Page 70: Abbreviations, Trademarks And Symbols
Chromatography Hertz International Electrotechnical Commission Inner Diameter Instructions for Use Installation Qualification In vitro diagnostics Kilogram Liter LC/MS Liquid Chromatography / Mass Spectrometry Light Emitting Diode Laboratory Information System 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 70/73... -
Page 71: Trademarks
The following product names and any registered and unregistered trademarks mentioned in this document are used for identification purposes only and remain the exclusive property of their respective owners: • Tecan and the Tecan Logo are trademarks of Tecan Group Ltd., Männedorf, Switzerland ® • Windows and Excel are registered trademarks of Microsoft Corporation, Redmond, WA, USA ®... -
Page 72: Tecan Customer Support
18.3 Tecan Customer Support If you have any questions or need technical support for your Tecan product, contact your local Tecan Customer Support organization. Go to http://www.tecan.com/ for contact information. Prior to contacting Tecan for product support, prepare the following information for the best possible technical support (see name plate): •... - Page 73 +49 1805 8322 633/ +49 1805 TECAN DE Fax +44 118 930 5671 Fax +49 7951 9417 92 Email helpdesk-uk@tecan.com Email helpdesk-de@tecan.com Tecan SP, Incorporated 14180 Live Oak Ave. Baldwin Park, CA 91706 253-5286-REV-L Resolvex A200 OPERATING MANUAL © Copyright Tecan SP Page 73/73...
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