Time Resolved Fluorescence - tecan Infinite 200 PRO Instructions For Use Manual

7.3.3

Time Resolved Fluorescence

Sensitivity
Measurement Parameters:
Plate Layout:
2016-04
For the Infinite 200 PRO with the option 'Fluorescence Top', the following tests
may be performed to prove the specifications:
•
Sensitivity
•
Precision/Repeatability
These test instructions are valid for the Infinite F200 PRO.
Perform the following measurement to determine the sensitivity:
Parameter
Reading Mode
Ex Wavelength
Em Wavelength
Number of flashes
Integration Time
Lag Time
Settle Time
Gain
Plate Type
Pipette 200 µl of 1 nM Europium solution or the blank solution (enhancement
solution) into the appropriate wells according to the plate layout:
<> 1 2
A
B
C
D
E
F
G
H
IFU for Infinite 200 PRO No. 30052730
Setting
Fluorescence Top
Infinite F200 PRO: 340 (35) nm
Infinite M200 PRO: 340 nm
Infinite F200 PRO: 612 (10) nm
Infinite M200 PRO: 617 nm
25
400
100
0
Optimal
GRE96fw
3 4 5
Rev. No. 1.6
7. Quality Control
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