tecan Infinite 200 PRO Instructions For Use Manual page 160

7. Quality Control
Precision
Measurement Parameters:
Plate Layout:
<> 1 2
A
B
C
D
E
F
G
H
Material/Reagents:
Calculation of Precision:
160
Perform the following measurement to determine the precision/reproducibility:
Parameter
Reading Mode
Ex Wavelength
Em Wavelength
Number of flashes
Integration Time
Settle Time
Gain
Plate Type
Part of the Plate
Kinetic
Interval Time
Pipette 200 µl of 1 nM Fluorescein or the blank solution (0.01 M NaOH) into the
appropriate wells according to the plate layout:
3 4 5
1 nM Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate black
200 µl Pipette + tips
stdev
=
Pr ecision ( CV %)
mean Average RFU value of well A1 over the 20 kinetic
wellA1
stdev Standard deviation of RFU values of Well A1 over the 20 cycles
wellA1
The result of the formula determines the Precision in % CV.
IFU for Infinite 200 PRO
Setting
Fluorescence Top
Infinite F200 PRO: 485 (20) nm
Infinite M200 PRO: 485 nm
Infinite F200 PRO: 535 (25) nm
Infinite M200 PRO: 535 nm
25
40
0
Optimal
GRE96fb
A1
20 Cycles
Minimal
6 7 8
* 100
wellA 1
mean
wellA 1
No. 30052730 Rev. No. 1.6
9 10 11
2016-04
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