tecan Infinite 200 PRO Instructions For Use Manual page 175

Measurement Parameters:
Evaluation:
2016-04
Parameter
Reading Mode
Ex Wavelength
Em Wavelength
Excitation Time
Integration Time
Gain
Plate Type
Calculate the average and standard deviation for each Omnibead concentration.
Perform a blank reduction by subtracting the average signal of the blanks wells
from the average signal of each Omnibead concentration.
Plot the average blank-corrected values against the final Omnibead
concentrations in a XY scatter diagram. Add a linear trend line with intercept set
to 0 and solve the trend line equation (y = kx) using the 3-fold standard deviation
of the blank as y.
y
x =
k
y 3* stdev of the blank (wells N2-P6)
Extrapolate the detection limit [ng/ml] by using the 3-fold standard deviation of the
blank as y.
IFU for Infinite 200 PRO No. 30052730
7. Quality Control
Setting
AlphaScreen/AlphaLISA
680 (30) nm
570 (100) nm
1000 ms
500 ms
Calculated from Well: A1
GRE384fw
Rev. No. 1.6 175