tecan Infinite 200 PRO Instructions For Use Manual page 163

Material/Reagents:
Calculation of Detection Limit (Sensitivity):
Uniformity
Measurement Parameters:
Plate Layout:
<> 1 2
A
B
C
D
E
F
G
H
Material/Reagents:
Calculation of Uniformity:
2016-04
1 nM Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate µClear, black with transparent bottom
200 µl Pipette + tips
See 7.3.1 Fluorescence Top: Sensitivity.
Perform the following measurement to determine the Uniformity:
Parameter
Reading Mode
Ex Wavelength
Em Wavelength
Number of flashes
Integration Time
Settle Time
Gain
Plate Type
3 4 5
Filling volume: 200 µl
25 nM Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate µClear, black with transparent bottom
200 µl Pipette + tips
See 7.3.1 Fluorescence Top: Uniformity
IFU for Infinite 200 PRO No. 30052730
Setting
Fluorescence Bottom
Infinite F200 PRO: 485 (20) nm
Infinite M200 PRO: 485 nm
Infinite F200 PRO: 535 (25) nm
Infinite M200 PRO: 535 nm
25
40
0
Optimal
GRE96fb
6 7 8
Rev. No. 1.6
7. Quality Control
9 10 11 12
163