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Summary of Contents for tecan RESOLVEX M10 48
- Page 1 OPERATING MANUAL RESOLVEX™ M10 48 MULTI-CHANNEL SPE PROCESSOR Operating Manual for RESOLVEX M10 48 P/N 279-1014 Rev C Page 1/18...
- Page 2 Table of Contents Product Overview ____________________________________________________3 Safety ______________________________________________________________4 Intended Use _________________________________________________________4 Safety Considerations __________________________________________________4 Disposal _____________________________________________________________4 2.3.1 Disposal of Packing Material _____________________________________________4 2.3.2 Disposal of Operating Material ___________________________________________5 2.3.3 Disposal of the unit ____________________________________________________5 Applicable Symbols ____________________________________________________5 Warning Symbols _____________________________________________________6 Laboratory Practices ___________________________________________________6 Site Preparation _______________________________________________________7 Gas Requirements ____________________________________________________7...
- Page 3 Product Overview The RESOLVEX M10 48 for Positive Pressure Solid Phase Extraction (SPE) offers state-of- the-art operation for SPE of individual columns containing sorbent beds of a few milligrams in 1mL tube sizes to larger bed sizes in 3, 6, and 20 mL tubes. Positive pressure processing of SPE columns exhibits a number of advantages compared to traditional vacuum manifold processing.
- Page 4 Follow laboratory procedures for biohazardous waste disposal according to national and local regulations This chapter provides instructions for how to lawfully dispose of waste material accumulating in connection with the Tecan RESOLVEX M10 48 CAUTION OBSERVE ALL FEDERAL, STATE AND LOCAL ENVIRONMENTAL REGULATIONS 2.3.1...
- Page 5 INQUIRE ABOUT APPROPRIATE COLLECTING POINTS AND APPROVED METHODS OF DISPOSAL IN YOUR COUNTRY, STATE, OR REGION. 2.3.3 Disposal of the unit If you have any questions concerning the disposal of the unit, please contact your local Tecan customer support. Pollution degree 2 (IEC/EN 61010-1)
- Page 6 Care should be taken to avoid injury to hands when in the vicinity of equipment with closing mechanical parts. Biological Risk WARNING DEPENDING ON THE APPLICATIONS, PARTS OF THE TECAN RESOLVEX MAY HAVE BEEN IN CONTACT WITH BIO-HAZARDOUS MATERIAL. MAKE SURE TO TREAT THIS MATERIAL ACCORDING TO THE APPLICABLE SAFETY STANDARDS AND REGULATIONS.
- Page 7 Site Preparation The RESOLVEX M10 48 unit should be placed in a laboratory and operated where there is proper ventilation or from inside a chemical fume hood when volatile solvents are used. The RESOLVEX M10 48 requires a clean, oil-free gas source and must also be located near the installation site.
- Page 8 Unpacking the RESOLVEX M10 48 The total weight of the package is approximately 40 lbs./18 kg and good safety practices should be performed when handling, moving, and unpacking the shipping boxes. The RESOLVEX M10 48 is typically shipped with the following items: ...
- Page 9 M10 48 need to be returned or shipped back to Tecan SP, a Certificate of Decontamination form must be submitted prior to shipping the unit. Please contact Tecan SP Customer Service at (+1) 626-962-0010 (option 3) for more information on returning or shipping the unit to Tecan SP.
- Page 10 SPE Rack Configurations and Use The RESOLVEX M10 48 provides the means to batch process solid phase extraction columns ® in the 48-position format. The CEREX column racks interface to several collection tube racks in the 48-position format. Depending upon the volume of the eluent, the appropriate collection rack or collection tray can be selected from the ancillary products available with the RESOLVEX M10 48.
- Page 11 Column Compression and Decompression The RESOLVEX M10 48 is designed to accept up to 48 columns, installed in a column rack. Because of the unique manifold design, each column/well is pressurized to the same level even if columns are omitted. Populate the SPE column rack and corresponding collection rack with columns and collection tubes.
- Page 12 Manifold Operation Each manifold port is flow-restricted in order to maintain uniform manifold pressure in all positions, even if some positions are not filled by columns. In addition, there are valves located on top of the manifold that shut off individual rows of 12. With no column in place, there is approximately 0.75 SCFH (0.35 liter per minute) of flow through a port at 25 PSIG (1.7 bar).
- Page 13 Pressure Application The RESOLVEX M10 48 has a two-level gas delivery system used to pressurize the SPE columns. It is user-controlled and operated manually by adjusting the pressure and flow each time the unit is used. One part of the system is controlled by the rotameter located on the left side of the panel. By switching the “FLOW CONTROL”...
- Page 14 Preventive Maintenance The RESOLVEX M10 48 Pressure is a rugged unit constructed of anodized aluminum, stainless steel, and solvent resistant plastics. However, the following daily and periodic maintenances should be observed. The column seal must be inspected daily for proper function of the unit. Failure to observe for detachment from manifold (peeling) and holes or rips in the column seal, may result in improper unit functionality.
- Page 15 ® Cerex Technology ® Cerex is a patented manufacturing technology that enables creation of very high performance ® Solid Phase Extraction (SPE) columns. Cerex technology provides for the use of microparticulate sorbents in the SPE column, typically in the 10µm particle size range, as compared to the 40µm particle size range used by most commercial suppliers.
- Page 16 This high capture effect allows the use of much ® smaller bed sizes in Cerex columns compared to conventional sorbents. For example, a 35mg, ® or even a 20mg Cerex column, typically exhibits the same effective capacity as a 100mg, 40µm SPE product.
- Page 17 Milliliter Millimeter Microplate μl Microliter Nonferrous Metal NIST National Institute of Standards and Technology Nanometer Optical Density Operational Qualification RoHS Restriction of the Use of Certain Hazardous Substances Standard Operating Procedure Arbitrary Units TÜV Technischer Überwachungsverein (Technical Inspection Agency) Operating Manual for RESOLVEX M10 48 P/N 279-1014 Rev C Page 17/18...
- Page 18 Email helpdesk-ch@tecan.com Customer Helpdesk United Kingdom Customer Helpdesk Germany Phone +44 118 930 0300 Phone +49 1805 8322 633/ +49 1805 TECAN DE Fax +44 118 930 5671 Fax +49 7951 9417 92 Email helpdesk-uk@tecan.com Email helpdesk-de@tecan.com Tecan SP, Incorporated 14180 Live Oak Ave.
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