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tecan RESOLVEX M10 96 Operating Manual

Multi-channel spe processor

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OPERATING MANUAL
RESOLVEX™ M10 96
and
RESOLVEX™ M10 96 XT
MULTI-CHANNEL SPE PROCESSOR
TM
Operating Manual for RESOLVEX
M10 96 & M10 96 XT
P/N 278-2036 Rev C
Page 1/19

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Summary of Contents for tecan RESOLVEX M10 96

  • Page 1 OPERATING MANUAL RESOLVEX™ M10 96 RESOLVEX™ M10 96 XT MULTI-CHANNEL SPE PROCESSOR Operating Manual for RESOLVEX M10 96 & M10 96 XT P/N 278-2036 Rev C Page 1/19...
  • Page 2: Table Of Contents

    Table of Contents Product Overview ____________________________________________________3 Safety ______________________________________________________________4 Intended Use _________________________________________________________4 Safety Considerations __________________________________________________4 Disposal _____________________________________________________________4 2.3.1 Disposal of Packing Material _____________________________________________4 2.3.2 Disposal of Operating Material ___________________________________________5 2.3.3 Disposal of the unit ____________________________________________________5 Applicable Symbols ____________________________________________________5 Warning Symbols _____________________________________________________6 Laboratory Practices ___________________________________________________6 Site Preparation _______________________________________________________7 Gas Requirements ____________________________________________________7...
  • Page 3: Product Overview

    All other users will need the standard M10 96 configuration For simplicity, the rest of this operating manual will refer to both product configurations as the RESOLVEX M10 96 Positive pressure processing of SPE columns exhibits a number of advantages compared to traditional vacuum manifold processing.
  • Page 4: Safety

    Follow laboratory procedures for biohazardous waste disposal according to national and local regulations This chapter provides instructions for how to lawfully dispose of waste material accumulating in connection with the Tecan RESOLVEX M10 48 CAUTION OBSERVE ALL FEDERAL, STATE AND LOCAL ENVIRONMENTAL REGULATIONS 2.3.1...
  • Page 5: Disposal Of Operating Material

    INQUIRE ABOUT APPROPRIATE COLLECTING POINTS AND APPROVED METHODS OF DISPOSAL IN YOUR COUNTRY, STATE, OR REGION. 2.3.3 Disposal of the unit If you have any questions concerning the disposal of the unit, please contact your local Tecan customer support. Pollution degree 2 (IEC/EN 61010-1)
  • Page 6: Warning Symbols

    Care should be taken to avoid injury to hands when in the vicinity of equipment with closing mechanical parts. Biological Risk WARNING DEPENDING ON THE APPLICATIONS, PARTS OF THE TECAN RESOLVEX MAY HAVE BEEN IN CONTACT WITH BIO-HAZARDOUS MATERIAL.  MAKE SURE TO TREAT THIS MATERIAL ACCORDING TO THE APPLICABLE SAFETY STANDARDS AND REGULATIONS.
  • Page 7: Site Preparation

    Site Preparation The RESOLVEX™ M10 96 unit should be placed in a laboratory and operated where there is proper ventilation or from inside a chemical fume hood when volatile solvents are used. The RESOLVEX™ M10 96 unit requires a clean, oil-free gas source and must also be located near the installation site.
  • Page 8: Installing The Resolvex™ M10 96

    The RESOLVEX™ M10 96 is typically shipped with the following items:  296-7501 RESOLVEX™ M10 96  278-2036 Operation Manual  279-0010 Installation Kit  278-0035 System 96 Column Seal, Removable Adhesive (6 month shelf life)  278-0021 96 Deep Well Collection Plate, 1.2mL x 96-Well ...
  • Page 9: Product Warranty

    (+1) 626-962-0010 (option 3). Should the RESOLVEX™ M10 96 need to be returned or shipped back to Tecan SP, a Certificate of Decontamination form must be submitted prior to shipping the unit. Please contact Tecan SP Customer Service at (+1) 626-962-0010 (option 3) for more information on returning or shipping the unit to Tecan SP.
  • Page 10: Tray Configurations

    Tray Configurations The RESOLVEX™ M10 96 provides the means to batch process solid phase extraction columns in the 96-position format. The RESOLVEX™ M10 96 will accommodate both the integrated and the modular SPE plates stacked onto a variety of collection plates. The modular SPE plate is a ®...
  • Page 11: Column Compression And Decompression

    The compression speed is pre-set and is not adjustable. The pressure applied to the compression system is pre-set and is not adjustable. Column Stacking Option for Positive Pressure Sample Processing Tecan SP’s column stacking for Positive Pressure sample processing (PN 253- 0009 and PN 253-0007) offers numerous options and techniques for sample preparation.
  • Page 12  The use of stacking plates is automatable on Tecan’s RESOLVEX A100 Stand-Alone Workstations and on industry-standard robotic liquid handlers. Operating Manual for RESOLVEX M10 96 & M10 96 XT P/N 278-2036 Rev C Page 12/19...
  • Page 13: Manifold Operation

    Manifold Operation Each manifold port is flow-restricted in order to maintain uniform manifold pressure in all positions, even if some positions are not filled by columns. With no column in place, there is approximately 0.75 SCFH (0.35 +/- .236 liter per minute) of flow through a port at 25 PSIG (1.7 bar). The principle of the restrictor manifold design is discussed in the following diagram.
  • Page 14: Pressure Application

    Pressure Application The RESOLVEX™ M10 96 has a two-level gas delivery system used to pressurize the SPE columns. It is user-controlled and operated manually by adjusting the pressure and flow each time the unit is used. One part of the system is controlled by the rotameter located on the left side of the unit panel. By switching the “FLOW CONTROL”...
  • Page 15: Daily Maintenance

    14.1 Daily Maintenance 1. Inspect the manifold column seal for wear and tear or detachment. Replace if necessary or clean using a lint-free cloth or wipe. 2. Wipe up all spills on the surface areas of the unit. Warning: Waste bin and surroundings may be contaminated with biological samples.
  • Page 16: Cerex ® Technology

    ® Cerex Technology ® Cerex is a patented manufacturing technology that enables creation of very high performance ® Solid Phase Extraction (SPE) columns. Cerex technology provides for the use of microparticulate sorbents in the SPE column, typically in the 10µm particle size range, as compared to the 40µm particle size range used by most commercial suppliers.
  • Page 17: Product Selection

    This high capture effect allows the use of much ® smaller bed sizes in Cerex columns compared to conventional sorbents. For example, a 35mg, ® or even a 20mg Cerex column, typically exhibits the same effective capacity as a 100mg, 40µm SPE product.
  • Page 18 Milliliter Millimeter Microplate μl Microliter Nonferrous Metal NIST National Institute of Standards and Technology Nanometer Optical Density Operational Qualification RoHS Restriction of the Use of Certain Hazardous Substances Standard Operating Procedure Arbitrary Units TÜV Technischer Überwachungsverein (Technical Inspection Agency) Operating Manual for RESOLVEX M10 96 &...
  • Page 19 Email helpdesk-ch@tecan.com Customer Helpdesk United Kingdom Customer Helpdesk Germany Phone +44 118 930 0300 Phone +49 1805 8322 633/ +49 1805 TECAN DE Fax +44 118 930 5671 Fax +49 7951 9417 92 Email helpdesk-uk@tecan.com Email helpdesk-de@tecan.com Tecan SP, Incorporated 14180 Live Oak Ave.

This manual is also suitable for:

Resolvex m10 96 xt