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Instructions for Use for HYDROFLEX PLUS Document Part No. 30199906 2022-01 Document Revision No. Firmware Level: 4.22...
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Tecan Customer Support If you have any questions or need technical support for your Tecan product, contact your local Tecan Customer Support organization. Go to http://www.tecan.com/ for contact information. Prior to contacting Tecan for product support, prepare the following information for the best possible technical support (see name plate): •...
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Tecan Austria GmbH assumes no responsibility for any errors that may appear in this publication. It is the policy of Tecan Austria GmbH to improve products as new techniques and components become available. Tecan Austria GmbH therefore reserves the right to change specifications at any time with appropriate validation, verification, and approvals.
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• [Product Name] Tecan® and the Tecan Logo are registered trademarks of Tecan Group Ltd., Männedorf, Switzerland • DNA Expert is a registered trademark of Techcomp Ltd., Hong Kong, China •...
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Catalogue number Serial number Unique Device Identification The UDI symbol identifies the data carrier on the label. WEEE symbol China ROHS symbol TÜV SÜD MARK MAXIMUM FILLING LEVEL WARNING! HEAVY WHEN FULL WASTE 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
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• COLLECT WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT SEPARATELY. WARNING RISK OF FIRE AND EXPLOSION! ETHANOL IS FLAMMABLE AND WHEN IMPROPERLY HANDLED CAN LEAD TO EXPLOSIONS. PROPER LABORATORY SAFETY PRECAUTIONS MUST BE OBSERVED. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
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Second Serial number Träge (Slow Blow Fuse) Torr – millimeter of mercury (mmHg) torr TYPE Name and type of instrument Universal Serial Bus Volt Volt ampere WEEE Waste electrical and electronic equipment 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
HYDROFLEX PLUS Intended Use ..................16 2.2.2 Principle of Operation ......................16 2.2.3 User Profile .......................... 17 2.2.4 HYDROFLEX PLUS Configurations and Options ..............17 Instrument Specifications ........................18 Instrument Description ........................20 2.4.1 Instrument Dimensions ......................21 2.4.2 Rear Panel Connections ...................... 22 2.4.3...
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8.9.2 Disposal of Packing Material ....................74 8.9.3 Disposal of Operating Material ..................... 75 8.9.4 Disposal of the HYDROFLEX PLUS ..................75 Trouble Shooting and Error Messages ..................77 Errors ..............................77 Error Messages ........................... 77 9.2.1 Standard Instrument: Error Messages ................. 77 9.2.2...
3. Observe all Warning and Caution notices in the IFU (see Warnings, Cautions and Notes on page 6 for a description of the notices used in this document). 4. Never open the HYDROFLEX PLUS while the instrument is plugged into a power source.
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VOLUME PER WELL, AS WELL AS INSUFFICIENT WASHING OF THE WELLS AND MAY SERIOUSLY AFFECT ASSAY PERFORMANCE. FOR DETAILS ON HOW TO ADJUST THE HYDROFLEX PLUS TO THE TYPE OF MICROPLATE OR STRIP-PLATE USED, SEE CHAPTER 6 SETTINGS MENU IN THIS MANUAL.
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IF FOAMING CONTINUES TO BE A PROBLEM, WE RECOMMEND SWITCHING TO A LARGER WASTE BOTTLE (NOT PROVIDED WITH THE HYDROFLEX PLUS) AND ADDITIONALLY INCREASE THE CONCENTRATION OF ANTI-FOAMING AGENT IN THE WASTE BOTTLE. TO HELP FACILITATE BREAKDOWN OF THE FOAM IN THE...
2. General General Introduction The HYDROFLEX PLUS is an instrument for strip-wise processing of microplates in the 96-well format. The instrument is designed for professional use only and should only be operated by trained personnel. CAUTION THE WASH RESULTS OBTAINED WITH THE HYDROFLEX PLUS ARE...
2.2.2 Principle of Operation The HYDROFLEX PLUS can be used to wash plates for a variety of test routines, mainly in ELISA applications. The HYDROFLEX PLUS is a microplate strip washer for automated washing, aspiration, dispensing, and shaking of 96-well microplates.
2. General volumes. The HYDROFLEX PLUS is running both the aspiration pump and the dispense pump in parallel. During a soak-step the HYDROFLEX PLUS supports linear shaking of the plate carrier. 2.2.3 User Profile Professional User - Administrator Level The administrator is a person who has suitable technical training and corresponding skills and experiences.
2. General CAUTION UNAUTHORIZED MODIFICATION OF THE HYDROFLEX PLUS, ANY OF ITS OPTIONS AND/OR COMPONENTS AS WELL AS ON ANY CORRESPONDING SOFTWARE OR SPARE PARTS WILL RESULT IN A LOSS OF WARRANTY AND A POTENTIAL LOSS IN INSTRUMENT PERFORMANCE. CAUTION...
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-20°C to 60°C (-4°F to 140°F) Relative Humidity Operation 20 % to 80 % non-condensing Storage 10 % to 80 % non-condensing Others Overvoltage category Pollution degree Method of disposal Electronic waste (infectious waste) 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
2. General Instrument Description The diagram below shows the main components of the instrument: Manifold Arm power Power ON Manifold Keyboard and Display Unit Guide Bar Prime Tray Plate Support TECAN hydro FLEX HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
2. General 2.4.1 Instrument Dimensions Standard Instrument – Dimensions 203 mm / 7,99" 290 mm / 11.42" 362,6 mm / 14.28" 275,2 mm / 10.83" 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
Declaration of Conformity on the last page of this document. All connected devices must be approved and listed as per IEC 60950-1 Information Technology Equipment – Safety or equivalent local standards. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
Marked Aspirating Pump Channel select Solenoid Valves HYDROFLEX PLUS Option The HYDROFLEX PLUS can be delivered with the following option: Liquid Level Detection The level of liquid is checked in the liquid bottle(s) (LLD) and in the waste bottle. An acoustic signal alerts the user when the bottles are empty or full.
Fig. 2.1 Waste and Liquid Bottles with LLD Sensors See chapter 3.3.1 Connection Diagram for further details. Microplate Requirements Only 96-well format microplates (round, v-shaped, and flat bottom - including strip plates) can be used with the HYDROFLEX PLUS. PARAMETERS CHARACTERISTICS Max. overall plate height 14.35 mm ±...
Installation When installing, moving, or connecting the instrument, follow the instructions in this publication. Tecan does not accept the responsibility for injury suffered by anyone attempting these operations without following the instructions in this publication, nor for damage incurred to the instrument.
8. Save packing materials for further transportation purposes. The HYDROFLEX PLUS must be shipped in the original packaging. 3.2.1 Unpacking and Inspection Checklist The standard HYDROFLEX PLUS is shipped in one carton, which contains: • Instrument Carton 1: • Accessory package which contains:...
Ensure that the on/off switch in the rear panel of the instrument is in the off position, then connect the instrument to the power supply: insert the main power cable into the main power socket on the rear of the instrument. 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
Sensors Connector Waste Tube Maximum filling level as indicated on bottle Liquid 1 Liquid 2 Liquid 3 Liquid 4 Waste Computer for Remote Control using the HydroControl software Fig. 3.2 Connection Diagram HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
TECAN AUSTRIA HYDROFLEX HYDROFLEX PLUS is the name of the instrument, which can be defined via the HydroControl software. If a Rinse procedure was aborted before the instrument was switched off, the...
4.2.1 Instrument Firmware Menus The instrument has the following menu options: Program Start, Define/Edit, Show, Clear Settings Edit Plates, Options, Bubble Sensor Procedures Prime, Rinse, Empty Prime Tray HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
BECOME CONTAMINATED (SEE 5.2.2 DEFINE/EDIT A PROGRAM (DEFINE/EDIT MENU)). WARNING WHEN THE INSTRUMENT IS BUSY, DO NOT TOUCH THE MANIFOLD! AFTER THE INSTRUMENT HAS BEEN USED, THE MANIFOLD AND PRIME TRAY MAY BE INFECTIOUS! 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
Z-Position Bottom Figure 4.2 Wash Position Diagrams MOVE OVER. MOVE CUST. Movement of Movement of manifold manifold Overflow Position Custom Position Bottom Position Bottom Position Figure 4.3 Movement Diagrams for Z-positions with MOVE HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
VOLUME PER WELL, AS WELL AS INSUFFICIENT WASHING OF THE WELLS AND MAY SERIOUSLY AFFECT ASSAY PERFORMANCE. FOR DETAILS ON HOW TO ADJUST THE HYDROFLEX PLUS TO THE TYPE OF MICROPLATE OR STRIP-PLATE USED, SEE CHAPTER 6 SETTINGS MENU IN THIS MANUAL.
To minimize cell detachment in drip mode, it is recommended to use a customized plate definition file with elevated z-position Bottom of approx. 7000 µm. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
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PROGRAM END? EXIT OK When the final aspiration step appears, press NO to finish FINAL ASP defining the program. Figure 4.6 Example of a typical wash program for working with adherent cells 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
6. Switch the instrument off. If the manifold is to be soaked overnight, do not switch the instrument off! HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
Clear The Program menu has the following structure: PROGRAM < > START DEFINE EDIT SHOW CLEAR < > EXIT OK < > EXIT OK < > EXIT OK < > EXIT OK 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
INIT STOP The current wash step will be displayed. Press stop to abort the program. WASH STOP PROGRAM When the program is finished, the < > instrument returns to the program menu. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
Add a final aspiration step if necessary. FINAL ASP? The parameters for the final aspirate step can be selected. NO YES START Once the program has been defined, the Start menu appears again. < > EXIT OK 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
IF NOT ALL WELLS IN A STRIP ARE FILLED WITH LIQUID. TO PREVENT CROSS CONTAMINATION THE HEAD SPEED SHOULD BE LOWERED. Aspiration Position Diagrams For further details, see also 4.5 Washing Positions. Position Overflow Position Custom Position Bottom Figure 5.1 Aspiration Position Diagrams HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
Z-Position Bottom Figure 5.2 Z-Position Diagrams MOVE OVER. MOVE CUST. Movement of Movement of manifold manifold Overflow Position Custom Position Bottom Position Bottom Position Figure 5.3 Movement Diagrams for Z-positions with MOVE 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
Press OK to define another wash step or press – or + to select the next program step, Program End or a new cycle. For further details, see also 4.5 Washing Positions. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
– or + to select the next program step, Program End or a new cycle. 5.2.7 Process Step: User Prompt Select and confirm User Prompt strip on CX PX USER PR. required position in current program. EXIT OK 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
If no final aspirate step was used, “CX CYCLEEND” appears. Press OK to view the cycle number. END OK Press END to exit the Show menu. Press - to return to the previous step. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
If the program is locked it cannot be cleared and the following message is displayed: PROGRAM IS LOCKED The program can only be unlocked via the HydroControl software, by users with the appropriate rights. 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
Press OK to save the defined position; Press EXIT to leave the menu without saving the position. The instrument moves to the home position and the firmware returns to the Edit Plates menu. EDIT PLATES EXIT OK 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
EXIT OK 6.1.3 Bubble Sensor BUBBLE SENSOR Press OK to continue. EXIT OK Press – or + to select between BUBBLE OFF OFF, LOW, MEDIUM and HIGH. EXIT OK Press OK to confirm. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
> EXIT OK 6.2.1 Rinse For details, see chapter 8.1 Rinsing. 6.2.2 Prime For details, see chapter 8.2 Priming. 6.2.3 Empty Prime Tray Press OK and the prime tray will be aspirated. 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
For details on how to adjust the HYDROFLEX PLUS to the type of microplate or strip-plate used, see chapter 6 Settings Menu of this manual.
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HYDROFLEX PLUS as described in this manual in 2.4.2 Rear Panel Connections. 3. Prime channel 1 of the HYDROFLEX PLUS for 10 seconds as described in 8.2 Priming. 4. Weigh empty and dry Greiner96ft specified above on lab-balance and record TARE.
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2. Fail: dispense accuracy is outside of the above-mentioned range. Troubleshooting the QC Procedure 1. If the HYDROFLEX PLUS has failed the above tests, perform thorough cleaning step using RINSE NIGHT procedure (see Rinse Night on page 59). 2. Disinfect manifold according to procedure described in chapter 8.8 Instrument Disinfection of this manual.
When Rinse Night is selected the Time cannot be set. Rinse Night must be performed with distilled water only. The Rinse menu has the following structure: RINSE < > RINSE DAY RINSE NIGHT < > < > 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
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The manifold remains immersed in the prime tray until End is pressed. Press END to finish the rinse procedure and the firmware returns to the PROGRAM Program menu. < > HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
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The manifold STOP END remains immersed in the prime tray until End is pressed. Press END to finish the rinse procedure and the firmware returns to the Program menu. PROGRAM < > 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
8. Maintenance and Cleaning Priming Priming is performed to fill the liquid system of the HYDROFLEX PLUS with liquid and to remove all air from the tubes. A priming step must also be performed when switching between buffers. If different wash buffers are used in a wash program, the instrument performs a prime step automatically before switching to the next liquid.
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> Select required channel CHANNEL 1 (1 - 4, depending on instrument configuration) EXIT OK Select OK and the prime procedure starts PROGRAM Instrument is ready to perform wash procedures < > 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
8.5 Preventive Maintenance Plan. CAUTION IF THE MANIFOLD IS NOT RINSED, THE NEEDLES WILL BECOME BLOCKED. IF THIS OCCURS, THE MANIFOLD WILL NEED EXPENSIVE REPAIRS OR WILL HAVE TO BE REPLACED. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
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WARNING WHEN HANDLING WASTE BOTTLES, IT IS ADVISABLE TO ADHERE TO APPLICABLE SAFETY PRECAUTIONS, (INCLUDING THE WEARING OF POWDER-FREE GLOVES, SAFETY GLASSES AND PROTECTIVE CLOTHING) TO AVOID POTENTIAL INFECTIOUS DISEASE CONTAMINATION. 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
MATERIAL. IF THE SPILL OCCURS IN THE INSTRUMENT, A SERVICE TECHNICIAN IS REQUIRED. Spilling of liquid or foam may occur when the HYDROFLEX PLUS is operated improperly such as: 1. Used microplate not matching installed manifold. 2. Positions of the strips in the strip-plate do not match the positions defined in the program used for processing.
2. Prime the instrument without liquid to empty the entire liquid system. 3. Check the filter(s) in the liquid bottle(s) for particles and rinse liquid filter(s) with distilled water. 4. Clean the plate carrier guide bar with 70% ethanol. 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
PRECAUTIONS, (INCLUDING THE WEARING OF POWDER-FREE GLOVES, SAFETY GLASSES AND PROTECTIVE CLOTHING) TO AVOID POTENTIAL INFECTIOUS DISEASE CONTAMINATION. WARNING PRIME THE INSTRUMENT WITHOUT LIQUID TO EMPTY ALL LIQUID FROM THE SYSTEM, BEFORE REMOVING THE MANIFOLD. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
The instrument can be used with the following types of manifolds: • Standard 8-way and • Standard 16-way The manifolds for the HYDROFLEX PLUS have the same basic components: Dispensing Tubes Locating Pin Locating Pin Aspirating Tubes Fig. 8.1 The Rear of the Manifold Note Always wear powder free gloves when handling the manifold.
2. Lift the manifold arm and remove the two screws that attach the manifold to the instrument using the Allen key provided. 3. Carefully pull the tubing off the connectors on the rear of the manifold and remove the manifold. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
4. Fit the dispensing tubes (marked blue) onto the top connector on the rear of the manifold. 5. Fit the aspirating tubes (marked red) onto the two bottom connectors on the rear of the manifold (marked with red labels). 3 screws 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
To reinstall the plate carrier, follow the procedure listed below: 1. Push the plate carrier so that it is fully inserted into the instrument. 2. Lower the plate carrier. 3. Lower the manifold arm. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
Decon neutracon (Decon Laboratories Limited) • Microcide SQ (Global Biotechnologies) • Ethanol WARNING RISK OF FIRE AND EXPLOSION! ETHANOL IS FLAMMABLE AND WHEN IMPROPERLY HANDLED CAN LEAD TO EXPLOSIONS. PROPER LABORATORY SAFETY PRECAUTIONS MUST BE OBSERVED. 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
10. Wash your hands with a mild detergent and then disinfect them. 11. Complete a disinfection certificate and attach it to the outside of the box so that it is clearly visible. (See below for an example of the disinfection certificate). HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
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Contact person ............................Company: ..............................Function: ..............................Phone/Fax: ..............................E-mail: ................................. Date of decontamination: ..........................Method of decontamination applied: ......................................................Date: ................................Signature: ..............................2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
8. Maintenance and Cleaning Disposal of Instrument 8.9.1 Introduction This chapter gives instructions on how to lawfully dispose of waste material accumulating in connection with the HYDROFLEX PLUS. CAUTION OBSERVE ALL FEDERAL, STATE AND LOCAL ENVIRONMENTAL REGULATIONS. ATTENTION DIRECTIVE 2012/19/EU ON WASTE ELECTRICAL AND ELECTRONIC...
INQUIRE ABOUT APPROPRIATE COLLECTING POINTS AND APPROVED METHODS OF DISPOSAL IN YOUR COUNTRY, STATE OR REGION. 8.9.4 Disposal of the HYDROFLEX PLUS Please contact your local Tecan service representative before disposing of the instrument. CAUTION ALWAYS DISINFECT THE INSTRUMENT BEFORE DISPOSAL. Pollution degree...
INIT ERROR Press OK to remove the error message and the instrument returns to the standby mode. Check that the plate carrier is correctly inserted and that the plate carrier system is clear. 2022-01 HYDROFLEX PLUS No. 30199906 Rev. No. 1.1...
If the waste bottle becomes full during the washing procedure, the instrument will beep; however, no error message is displayed during or after the instrument has completed the washing procedure. CAUTION THE INSTRUMENT DOES NOT STOP A PROCEDURE WHEN THE WASTE BOTTLE BECOMES FULL. HYDROFLEX PLUS No. 30199906 Rev. No. 1.1 2022-01...
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IF FOAMING CONTINUES TO BE A PROBLEM, WE RECOMMEND SWITCHING TO A LARGER WASTE BOTTLE (NOT PROVIDED WITH THE HYDROFLEX PLUS) AND ADDITIONALLY INCREASE THE CONCENTRATION OF ANTI-FOAMING AGENT IN THE WASTE BOTTLE. TO HELP FACILITATE BREAKDOWN OF THE FOAM IN THE...
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