tecan Columbus Washer Instructions For Use Manual
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tecan Columbus Washer Instructions For Use Manual

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TECAN
Instructions for Use for
Columbus Washer
Document Part No. I 109 006
May 2003
Document Revision : 2.2
Firmware Level: 4.x

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  • Page 1 TECAN Instructions for Use for Columbus Washer Document Part No. I 109 006 May 2003 Document Revision : 2.2 Firmware Level: 4.x...
  • Page 2 Tel.: +1 919 361 5200 Tel.: +44 11 89 300 300 Fax: +1 781 306 0837 Fax: +1 919 361 5201 Fax: +44 11 89 305 671 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...
  • Page 3 FAX: 06246/72770 E-mail: office.austria@tecan.com Copyright Information The contents of this Instructions for Use (IFU) are the property of Tecan Austria Ges.m.b.H and are not to be copied, reproduced or transferred to another person or persons without our prior written permission.
  • Page 4 Indicates a possibility of instrument damage or data loss if instructions are not followed. WARNING INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT FOLLOWED. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 5: Table Of Contents

    Procedure to Wash Individually Selected Strips ........ 4-5 Instrument with Printer and Barcode Reader Options ....... 4-6 Fill Verification..................4-7 Drip Mode Option (Cell Wash) ............4-9 384 Well Manifold Option..............4-9 May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...
  • Page 6 (without Aerosol Aspiration and Fill Verification) ........7-4 384 Well Manifold ................7-4 Manifold for Instrument with Aerosol Aspiration .........7-5 Manifold for Instrument with Fill Verification ........7-5 7.1.3 Exchanging the Plate Support ............7-6 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...
  • Page 7 Duration Test ..................9-4 Function Test ..................9-4 Select Options..................9-4 Adjust Dispensing Pump..............9-4 Adj. Prime Pos................... 9-4 Adj. Plate Ref..................9-4 Index May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 9: Safety

    Never open Columbus while the instrument is plugged into a power source. Observe proper laboratory safety precautions, such as wearing protective clothing and using approved laboratory safety procedures. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 11: General

    Before the instrument is installed and switched on, it should be left to stand for at least three hours, so there is no possibility of condensation causing a short circuit. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 12: Instrument Specifications

    Main fuse 2 x 1.6 T Amp. (slow blow) Physical Outside dimensions Width : 28,5 cm, Depth : 39,5 cm, Height:: 22,0 cm Weight 8.2 kg Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...
  • Page 13 The instruments conform to the legal norms at date. For more information, see the Declaration of Conformity Certificate on the last page of this IFU. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 14: Instrument Description

    Remote Control All connected devices must be proved and listed with regulations EN 60950, UL 1950 or CSA C22.2 No. 950 for Data Processing Devices Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 15: Connection Diagram

    (optional) Printer (option) Marked Wash Rinse Waste Liquid 1 solution Bottle Computer for Remote Control grey Bottle Holders with liquid sensor green for each bottle May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 16: Instrument Options

    3.23 onwards. Factory installation required! Automatic detection for the type of manifold fitted: Manifold Detection For 96 Well plate: M8, M12, M16. For 384 Well plate: only M32. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...
  • Page 17 This option is used for washing the 384 well microplate in 384 Well Manifold rows and can only be performed with a 32 way Manifold. (starting at 4.x) May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 18: Liquid System Diagram

    Fig. 2-4 Columbus Washer Dispense Solenoid Manifold Valve Marked blue Marked red Dispensing Pump Marked yellow Marked Aspirating Pump Marked yellow Channel select Solenoid Valves Aerosol Pump (optional) Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 19: Instrument Software Features

    Procedure to show the parameters for a program Show Adjust Procedure to adjust the instrument parameters Service Selection of the Service submenu For service engineer only May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 20: Washing Sequence Modes

    Dilution washing consists of a sequence of aspiration and dispense steps. Fig. 2-5 Washing Positions Overflow Washing Bottom Washing Dilution Washing Dispense step Aspiration step 2-10 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 21: Aspirating Positions

    The Rinse modes are: Rinse: Soak Rinse: Day Rinse: Night Rinse: Auto See Chapter 7 for description of the Rinse modes and how they are used. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 2-11...

  • Page 23: Installation Procedure

    3 x 2.5 l bottles for the standard instrument Fig. 3-1 The packaging for Columbus Washer Accessory package Liquid Sensor System and operating manual (optional) Instrument Bottles May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 24: Unpacking Procedure

    The washer requires 2 x T 1.6 A / 250 V (slow blow) fuses. WARNING TO PREVENT THE RISK OF FIRE, THE MAINS FUSES SHOULD ONLY BE REPLACED WITH THE SAME TYPE AND RATING OF FUSE. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 25: Installation Procedure

    (maximum 1 meter). Fig. 3-2 Connecting the Solution Tubes Inlets for channels 1 - 4 Aerosol Tube Waste Tube (optional) yellow May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 26: Fitting And Connecting The Liquid Sensors (Optional)

    Liquid Sensor Fix the tube support in the bottle holder as shown below. Tube support Insert the tubes and connect the bottle holder to the instrument. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 27: Connecting The Barcode Reader (Optional)

    Ensure that the on / off switch in the back panel of the instrument is in the off position. Insert the mains power cable into the mains power socket on the rear of the instrument. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 29: Operating Instructions

    The instrument must be primed before it can be used. Please ensure that the dispensing and aspiration pumps are not run for longer than a few minutes without liquid or they will be damaged. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 30: Mode Selection Menu

    Adjust Menu other yes Procedure to adjust the instrument parameters Service other yes Selection of the Service submenu For service engineers only Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 31: Priming Procedure

    Instrument ready to wash microplate XX yyyyyyyy + other yes Caution Do not use the instrument to aspirate or dispense any acidic solutions as this could damage the instrument. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 32: Operating Instructions

    The operating procedure is dependent on the instrument options and the program settings. Note: The washing of the 384 Well plates can only be performed with a 32 way Manifold. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 33: Procedure To Wash Individually Selected Strips

    Waste bottle empty Waste Bottle OK? exit Procedure performed Remove the microplate from plate support, XX yyyyyyyy instrument ready to wash next microplate + other yes May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 34: Instrument With Printer And Barcode Reader Options

    + other yes Caution When the instrument is busy, do not touch the manifold. After the instrument has been used, the manifold may be infectious. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 35: Fill Verification

    This can occur when the well was not or not completely aspirated, due to a blocked aspirating needle / tube or the general aspiration being too weak. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...
  • Page 36 1:20 - 14.2 mS/cm Beta IgM: Wash buffer MUREX: 1:20 - 7.8 mS/cm Wash fluid (Glycine/Borate): Wash buffer BIOTEST ELPHA: 1:10 - 28.1 mS/cm (Nr. 3 + 4): Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 37: Drip Mode Option (Cell Wash)

    384 Well Manifold, then the Wash procedure should be programmed by using the individual program steps: Asp. and Disp. The process step "Wash" should not be used. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 38: Error Messages

    Press yes (exit) to remove the error message and the instrument returns to the standby mode. Check that the plate support is correctly inserted and that the plate support system is clear. 4-10 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 39: Instrument With Liquid Sensor (Optional)

    If the liquid bottle is empty before a program is started, the following message is displayed: Liq. Empty Ch: X Fill the liquid bottle and press yes to remove the message and continue with the program. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 4-11...

  • Page 40: Instrument Fitted With Printer (Optional)

    If the instrument detects that one or more needles are not dispensing solution or non-conductive liquid is being used, the following error messages are scrolled on the display: Inadequate Wash! Exit Check Disp. Path Exit 4-12 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...
  • Page 41 Use the cleaning needles to clean the aspirating needles (see chapter 7) or replace the pump. Contact the service engineer to correct the problem. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 4-13...

  • Page 42: End Of Operation

    Place the wash solution tube into a bottle containing distilled water. Use the Rinse: Day rinsing procedure to rinse the wash system. When the rinsing procedure is completed, switch the instrument off. 4-14 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 43: Programming Procedure

    Dispense a solution • Soak: Leave the solution in the wells for a time, with or without shaking. • End: End the programming procedure for this cycle. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 44: Procedure For Defining A New Program

    Dispense Dispense a solution Soak Leave the solution in the wells for a time with or without shaking End the programming procedure for this cycle. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 45: Process Step: Aspirate

    384 Well Manifold, then the Wash procedure should be programmed by using the individual program steps: Asp. and Disp. The process step "Wash" should not be used! May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 46: Process Step: Dispense

    • Linear Shaking Select the shake speed: 1, 2 or 3. Confirm with yes . Select the shake mode: low, normal or high. Confirm with yes . Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 47: Process Step: End

    If printer option fitted, select setting for Print Prot.? ASK exit yes protocol printing (YES, NO, ASK) Instrument ready to perform defined X:yyyyyyy + other yes washing procedure May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 48: Editing A Defined Program

    Run XX:yyyyyyy - + other The instrument is now ready to use the program, which has been edited. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 49: Show Program

    Select required process step to be shown XXXX + Exit using - and + keys or press yes to step through all steps and settings Show XX:yyyyyyy + other yes May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 50: Clear Program

    This option is not to be confused with the Clear ALL function in the Service menu. See chapter 9 for more details. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 51: Adjust Menu

    Adjustments can be made to the switch thresholds for the bottles containing the wash, rinse and waste liquids. Note: The Adj. Liq. Sens. Option is only usable with the Liquid Sensor System option. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...
  • Page 52 0 = values below limit exit yes Press exit to continue the adjustment of other sensors. Exit Adjust Exit Adjust menu other yes XX yyyyyyyy + other yes Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 53: Plate Type

    Use - and + keys to move the manifold so that the exit yes aspirating needles are in the correct position for the overflow washing procedure Select plate type to be defined Plate: + other yes May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 54: Flat Bottom Plates

    Use - and + keys to move the manifold so that the exit yes aspirating needles are in the correct position for the overflow washing procedure Plate: Select plate type to be defined + other yes Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 55: Movement Diagrams

    Aspirate Position (2) Flat Bottom Wells Only Instrument Front Aspirate Bottom Position Instrument Front Instrument Front Bottom Wash Position Instrument Front Instrument Front Overflow Wash Position Instrument Front Instrument Front May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 56: Adjust Liquid Sensors (Optional)

    Press exit to continue the adjustment of other sensors. Use the cursor keys to repeat this procedure for the grey (rinse solution) and red (waste) bottles. See overleaf for more details. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 57: Liquid Sensors 1 - 2

    When all the sensors have been set, press exit and the following message is displayed: Adj.Liq.Sens.: other yes Press other to proceed to the next option. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 59: Maintenance And Cleaning

    Lift the manifold arm and remove the two screws that hold the manifold onto the holder. Fig. 7-1 The Manifold Screws When the two screws have been removed, carefully pull the manifold away from the holder. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 60: Manifold Cleaning

    Fill Verification option. Manifolds with the Fill Verification option should only be cleaned in an ultrasonic bath. If additional cleaning is required, use a small brush. Do not use autoclaving. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 61: Exchanging The Manifolds

    If the Fill Verification option is fitted, connect the small ribbon cable onto the connector block on the left hand side of the circuit board in the manifold arm. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 62: Manifold For The Standard Instrument (Without Aerosol Aspiration And Fill Verification)

    Manifold Type Indicator 384 Well Manifold Fig. 7-4 Rear View of the 384 Well Manifold Dispensing Tube Connector blue Locating Pin Locating Pin Aspirating Tube Connectors Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 63: Manifold For Instrument With Aerosol Aspiration

    For a 8 or 12 manifold only use connector 2 When all the required tubing and ribbon cable have been connected, lower the manifold arm. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 64: Exchanging The Plate Support

    To reinstall the plate support follow the procedure listed below: Push the plate support so that it is fully inserted into the instrument. Lower the plate support. Lower the manifold arm. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 65: Replacing The Mains Fuses

    T 1,6 A / 250 V (slow blow) Replace the fuse compartment. Reconnect the power cord and switch the instrument on WARNING IF THE FUSE CONTINUES TO BLOW, PLEASE CALL FOR SERVICE. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 66: Cleaning Procedures

    If the manifold is not rinsed, the needles will become blocked. This will result in the manifold either needing expensive repairs or it could become completely ruined. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 67: Preventive Maintenance Plan

    When using the reader, use the Windows based QC-Col Software to check quality control test dispensing and aspirating accuracy. • Download the specific saved programs to the washer. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 68: Rinse Modes

    Manifold left in solution until Stop key pressed Soaking Stop Standby mode XX:yyyyyyy Washing procedure can be performed + other yes 7-10 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 69: Rinse: Day

    Manifold left in solution until Soaking Stop key pressed Stop Standby mode XX:yyyyyyy + other yes Washing Procedure can be performed May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 7-11...

  • Page 70: Rinse: Night

    The repeat time can be set in the Adjust menu between 1 and 24 hrs. 7-12 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 71: Instrument Disinfection

    Prime the liquid system with disinfectant. Perform the Rinse - Night procedure with disinfectant solution and stop the procedure after five hours. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 7-13...
  • Page 72 Name:........................Firm: ........................Address: ........................................................................Country:....................... Signature:......................7-14 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 73: Disposal Of Instrument

    Follow laboratory procedures for biohazardous waste disposal, according to country specific regulations. Caution Always disinfect the instrument before disposal. Pollution degree Contaminated Waste Method of Disposal May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 7-15...

  • Page 75: Trouble Shooting

    No or incorrect Dispensing needles blocked dispensing No or incorrect Aspirating needles blocked aspirating Manifold Arm Error The manifold arm does not move up and down May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 77: Service Menu

    FOR AUTHORIZED SERVICE ENGINEERS ONLY! Select Options For authorized service engineers only. Adj. Prime Pos. For authorized service engineers only. Adj. Plate Ref. For authorized service engineers only. May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...
  • Page 78 For service engineer only yes exit no Adj.Plate Ref. For service engineer only yes exit no Service End Exit Service menu yes other Standby Mode Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 79: Skip Messages

    You should Rinse Press show to display message show exit skip Press skip to omit message Skip Messages: Duration Test Service yes exit no other yes May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2...

  • Page 80: Duration Test

    NEVER USE THE Adj Dispens Pump OPTION! FOR AUTHORIZED SERVICE ENGINEERS ONLY! Adj. Prime Pos. For authorized service engineers only. Adj. Plate Ref. For authorized service engineers only. Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 May 2003...

  • Page 81: Index

    Cleaning............ 7-2 Exchanging ..........7-3 Removal............ 7-1 Wash ............5-3 Washing Modes ......... 2-10 Option 384 Well Manifold ........4-9 Drip Mode ..........4-9 May 2003 Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2 Index...

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