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tecan NanoQuant Plate Quick Manual

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NanoQuant Plate™
Quick Guide

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  • Page 1 NanoQuant Plate™ Quick Guide...

  • Page 2: Table Of Contents

    Table of Contents General Information ..............3 Introduction ............... 3 Contents of the NanoQuant Plate Package....3 Computer Requirements ..........4 System Requirements ............5 Applications ..............6 Measurement Procedure............... 7 Software Installation Procedure ........7 Performing a Measurement ..........7 File menu .................

  • Page 3: General Information

    The plate has been designed to meet the requirements of research laboratories working with various types of low-volume samples including fluorophore-labeled nucleotides. Contents of the NanoQuant Plate Package The NanoQuant Plate package for Infinite reader series contains the following items: • NanoQuant Plate •...

  • Page 4: Computer Requirements

    .NET Framework. Windows If this version is not present, the install/upgrade Installer program will install it. 2002, 2003, 2007, 2010 (32-bit) – Starter edition NOT Microsoft Excel supported! Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4 2013-06...

  • Page 5: System Requirements

    Position 2: 280 nm (3 nm bandwidth) • Position 3: 340 nm (10 nm bandwidth) Note Only use the Infinite reader and the NanoQuant Plate at room temperature and under normal laboratory conditions. 2013-06 Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4...

  • Page 6: Applications

    2 μl is sufficient for accurate results. Absorbance of nucleic acid samples is measured at 260 nm. The optical path length of the NanoQuant Plate is 0.5 mm. To assess the purity of the nucleic acid, an additional measurement at 280 nm is performed to indicate proteins in the sample.

  • Page 7: Measurement Procedure

    Switch on and connect to Infinite instrument. Note i-control is delivered with the Infinite reader series. Performing a Measurement For applications using the NanoQuant Plate, a tab called Applications is implemented in i-control software, so that all measurements can be performed quickly and easily. Start i-control.
  • Page 8 10. When all settings are correct, click the Start Blanking button to initialize the blanking measurement. The plate transport moves out and the user is requested to insert the NanoQuant Plate with the respective blanking buffer. Quick Guide NanoQuant Plate No.30035094 Rev No.
  • Page 9 Figure 2: Correct orientation of NanoQuant Plate in the reader 11. The first measurement step is blanking with the required buffer. A separate window opens and the blanking procedure can be observed. 12. The blanking measurement is started and can be monitored in the measurement progress window.
  • Page 10 Click to start the measurement. • If no further measurement is to be performed, click No. An extra sheet appears in the Excel workbook summarizing the results of all previous measurements. Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4 2013-06...
  • Page 11 Figure 4: Automatic summary in Excel of all measured samples 18. When all measurements are completed, clean the NanoQuant Plate thoroughly and store it appropriately. 19. Once i-control has been closed, the Infinite reader can be switched off. Note Excel workbooks containing the measurement results are not saved automatically.
  • Page 12 If the correct fluorophores are selected, the samples can be applied into the NanoQuant Plate. Individual Blanking and Average Blanking The user may choose between two distinct blanking options: Average Blanking and Individual Blanking (set by default).
  • Page 13 Figure 7: Individual Blanking information in the Excel result sheet Note The CV value displayed in the Last Blanking box (see Figure 11) represents the variation coefficient of all wells used for blanking. 2013-06 Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4...
  • Page 14 Sample IDs may also be copied from Excel sheets and pasted into sample ID list. “Show Raw Data” To view all measurement results as raw values, the NanoQuant tab in the Results Presentation dialog box contains the Show Raw Data check box. Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4 2013-06...

  • Page 15: File Menu

    Move plate out Move plate in Show or hide the info pane Open the i-control help file. Sample The following sample types can be selected: • dsDNA • ssDNA • • Others 2013-06 Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4...
  • Page 16 The selected sample in Edit Sample must always have the ratio wavelength set at 280 nm, otherwise no marker can be selected for Dye 1 or Dye 2 and an error message appears. Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4 2013-06...

  • Page 17: Quality Control Of Nanoquant Plate

    Quality Control of NanoQuant Plate Average Blanking Out of Range (CV ≥ 10 %) If Average Blanking is out of range, the failed wells are highlighted in pink and an error message appears requesting the user to repeat blanking measurement.
  • Page 18 Figure 12: Color code for blanking procedure when re-selected: purple wells indicate formerly pink wells Move the plate out, repeat the cleaning procedure and apply fresh blanking samples into the plate and start blanking again. Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4 2013-06...

  • Page 19: Nanoquant Plate

    340 nm (10 nm bandwidth) reference Measurement time of whole plate Quantify Nucleic Acid Infinite 200 PRO: 1.15 minute for 16 samples Labeling Efficiency Infinite 200 PRO: 2 minutes for 16 samples 2013-06 Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4...
  • Page 20 Ratio 260/280 (50 μg/ml) ± 0.07 Precision @ 260 nm < 0.2 % Accuracy @ 260 nm < 0.5 % Figure 13: Technical drawing of the NanoQuant Plate (dimensions in mm) Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4 2013-06...

  • Page 21: Handling And Cleaning

    (wet) Kimwipe. The cleaning and maintenance procedures are important in order to prolong the NanoQuant Plate’s lifespan and to reduce the need for servicing. It is recommended to store the cleaned NanoQuant Plate in the aluminum storage box.

  • Page 22: Applying Samples

    Always use optimal tips for the multi channel pipette to ensure precise and consistent application of the samples. Figure 14: Applying samples onto the NanoQuant Plate If you need help applying the samples precisely into the wells with a multi channel pipette, use our pipetting aid: Place the pipetting aid with the indentation downward onto the quartz wells.

  • Page 23: Nanoquant Plate Disinfection

    OD values! NanoQuant Plate Disinfection All parts of the NanoQuant Plate that come in contact with patient samples, positive control samples or hazardous material must be treated as potentially infectious areas. Spray or apply 70% ethanol, which is commonly used for laboratory cleaning, over the whole plate.

  • Page 25: Calculations

    340 nm is used. Calculation of Nucleic Acid Concentration After the absorbance measurements of the nucleic acids have been performed in the NanoQuant Plate, i-control and Excel automatically calculate the nucleic acid concentration according to the Lambert-Beer law, including the reference values.
  • Page 26 – Abs [OD] blank A1 260 A1 310 A1 = Abs – Abs [OD] blank A2 260 A2 310 A2 = Abs – Abs [OD] blank B1 260 B1 310 B1 etc. Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4 2013-06...
  • Page 27 = (Abs – Abs ) – Abs [OD] 260 A2 340 A2 blank average = (Abs – Abs ) – Abs [OD] 260 B1 340 B1 blank average etc. 2013-06 Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4...
  • Page 28 310 nm for Infinite M200 PRO and Infinite M1000 (or 340 nm for Infinite F200 PRO and Infinite F500). The corrected absorbance values are used for the 260/280 ratio calculation. Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4 2013-06...

  • Page 29: About The Quick Guide

    Every effort has been made to avoid errors in text and diagrams; however, Tecan Austria GmbH assumes no responsibility for any errors that may appear in this publication. It is the policy of Tecan Austria GmbH to improve products as new techniques and components become available. Tecan Austria GmbH therefore reserves the right to change specifications at any time with appropriate verification, validation, and approvals.
  • Page 30 Microsoft Corporation, Redmond, WA, USA Copyright Information The contents of this document are the property of Tecan Austria GmbH and are not to be copied, reproduced, or transferred to another person or persons without prior written permission.
  • Page 31 Caution: Indicates a possibility of product damage or data loss if instructions are not followed. Warning: Carefully read and follow the instructions provided in this document before using the software or using the product. Symbols Manufactured by 2013-06 Quick Guide NanoQuant Plate No.30035094 Rev No. 1.4...

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