Definition Of The Detection Limit; Fluorescein (Fluorescence Intensity) Top; Fluorescein (Fluorescence Intensity) Bottom; Europium (Time Resolved Fluorescence) - tecan Infinite 200 PRO Instructions For Use Manual

6.3.1

Definition of the Detection Limit

6.3.2

Fluorescein (Fluorescence Intensity) Top

6.3.3

Fluorescein (Fluorescence Intensity) Bottom

6.3.4

Europium (Time Resolved Fluorescence)

2016-04
The detection limit is the fluorophore concentration where the background-
subtracted signal equals 3 times the standard deviation of the background noise.
When selecting 1 flash per well, the plate carrier does not stop at the
measurement position. Using more flashes per well may improve the detection
limit, but the total measurement time will be longer.
Plate Type (number of wells)
Dispensed Volume [µl]
Flashes (Reads) per Well
Fluorescein Detection Limit [pM]
Uniformity at 25 nM Fluorescein
Reproducibility at 25 nM Fluorescein
Plate Type (number of wells)
Dispensed Volume [µl]
Flashes (Reads) per Well
Fluorescein Detection Limit [pM]
Uniformity at 25 nM Fluorescein
Reproducibility at 25 nM Fluorescein
Plate Type (number of wells)
Dispensed Volume [µl]
Flashes (Reads) per Well
Europium Detection Limit
(Infinite F200 PRO)
Europium Detection Limit
(Infinite M200 PRO)
IFU for Infinite 200 PRO No. 30052730
6. Instrument Features
96
200
25
< 20 pM
< 3 % CV
< 2 %
96
200
25
Standard: 100 pM
Enhanced:
Infinite M200 PRO: 45 pM
Infinite F200 PRO: 35 pM
< 3 % CV
< 2 %
96
200
25
< 150 fM
< 5 pM (Typical value)
Rev. No. 1.6 139