tecan Infinite 200 PRO Instructions For Use Manual page 166

7. Quality Control
Material/Reagents:
Calculation of Detection Limit (Sensitivity):
Precision
Measurement Parameters:
Plate Layout:
Calculation of Precision:
166
1 nM Europium (B119-100, HVD Live Sciences)
Enhancement Solution (=Blank) (1244-105, HVD Live Sciences)
1 Greiner 96-well plate white
200 µl Pipette + tips
See 7.3.1 Fluorescence Top: Sensitivity.
Perform the following measurement to determine the precision/reproducibility:
Parameter
Reading Mode
Ex Wavelength
Em Wavelength
Number of flashes
Integration Time
Lag Time
Time between Move and Flash
Gain
Plate Type
Part of the plate
Kinetic
Interval Time
See 7.3.3 Time Resolved Fluorescence Precision.
See 7.3.1 Fluorescence Top: Precision.
IFU for Infinite 200 PRO
Setting
Fluorescence Top
Infinite F200 PRO: 340 (35) nm
Infinite M200 PRO: 340 nm
Infinite F200 PRO: 612 (10) nm
Infinite M200 PRO: 617 nm
25
400
100
0
Optimal
GRE96fw
A1
20 Cycles
Minimal
No. 30052730 Rev. No. 1.6 2016-04