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tecan MagicPrep NGS Operating Manual

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Operating Manual
Operating Manual
MagicPrep™ NGS Instrument
M01534_v1.1 MagicPrep NGS Instrument Operating Manual
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Summary of Contents for tecan MagicPrep NGS

  • Page 1 Operating Manual Operating Manual MagicPrep™ NGS Instrument M01534_v1.1 MagicPrep NGS Instrument Operating Manual Page 1 of 39...
  • Page 2 Operating Manual Document Change History Title: Part Number: M01534 MagicPrep NGS Instrument Operating Manual Translated from: n.a. Version: Issue: Document History: 2022-04-12 Initial Release 2022-04-22 Update electronic environmental mark for China M01534_v1.1 MagicPrep NGS Instrument Operating Manual Page 2 of 39...

  • Page 3: Table Of Contents

    Areas of Use / Area of Application ___________________________________________5 Improper Use ___________________________________________________________5 Warranty _______________________________________________________________6 Trademarks ____________________________________________________________6 Reference Documents ____________________________________________________6 1.8.1 MagicPrep NGS Reagent Kit User Guides ____________________________________6 Compliance with Laws and Standards ________________________________________6 1.10 Document Conventions ___________________________________________________6 Safety ________________________________________________________________8 Safety Message Conventions ______________________________________________8 2.1.1...
  • Page 4 Operating Manual 6.5.2.1 Event Log ________________________________________________________28 6.5.2.2 Export Log files ____________________________________________________28 6.5.3 Errors ________________________________________________________________29 MagicPrep NGS Instrument Functional Test __________________________________34 6.6.1 Starting the run: ________________________________________________________34 6.6.2 Quantify the libraries: ____________________________________________________34 6.6.3 Specifications: _________________________________________________________34 Support ______________________________________________________________35 System Care __________________________________________________________36 Decontamination _______________________________________________________36...

  • Page 5: About This Manual

    The system is For Research Use Only, Not for use in diagnostic procedures. Areas of Use / Area of Application The MagicPrep NGS system can only be used for the fully automated preparation of next generation sequencing (NGS) libraries using the specific Tecan Genomics MagicPrep reagent kits.

  • Page 6: Warranty

    Warranty The MagicPrep NGS instrument must not be used with components that are not approved by Tecan The use of unapproved components may impair the safety concept of the MagicPrep NGS instrument. The use of unapproved components would invalidate any warranty of safety and compliance to national and international standards, as required for NRTL certification, by EU directives, etc.
  • Page 7 Operating Manual For safety conventions and symbols refer to chapter “Safety” [2] The illustrations may show component versions which are not relevant to Illustrations your MagicPrep NGS instrument. M01534_v1.1 MagicPrep NGS Instrument Operating Manual Page 7 of 39...

  • Page 8: Safety

    Operating Manual 2 Safety This chapter describes the safety concept of the MagicPrep NGS instrument, provides general rules of correct behavior, and warnings concerning health hazards associated with the use of the MagicPrep NGS instrument. Safety Message Conventions 2.1.1 Signal Words Tab.

  • Page 9: General Safety Consideration

    This manual must always be available to all persons performing the tasks described herein. • Legal regulations, such as local, state and federal laws concerning the use or application, as well as the handling, of dangerous materials in connection with the MagicPrep NGS must be strictly followed. •...

  • Page 10: Operating Company

    65 hrs. of the preparation being completed. User Qualification The laboratory personnel must be fully qualified and trained to operate the MagicPrep NGS instrument. The work described in this Operating Manual must only be performed by authorized personnel with the qualifications prescribed below.

  • Page 11: Operator

    Helping the operator to solve problems with the system Safety Elements When the front door of the MagicPrep NGS instrument is open, do not insert your hand(s) into the instrument. When opening the Magic Prep NGS front door and inserting or removing the reagent cartridge and/or sample deck, please take care not to insert your hand(s) into the instrument.

  • Page 12: Decontamination Declaration

    The owner of the instrument has full responsibility for the effective decontamination of all the equipment. Before returning the MagicPrep NGS instrument to to Tecan, the owner of the instrument must complete and sign the Decontamination Declaration form, confirming that the decontamination has been performed in accordance with good laboratory guidelines.

  • Page 13: Technical Data

    Operating Manual 3 Technical Data Type Label The Type plate is on the rear of the MagicPrep NGS instrument and contains the following information: Identification data Model REF: Ordering information and revision level SN: Serial number Date of manufacture (YYYMMDD) Technical data U.f:: Supply voltage (Volts), frequency (Hertz)

  • Page 14: Power Supply

    Data and Power Connections Touch Screen Manual interlock override Door MagicPrep NGS power input Ethernet interface USB Interface Environmental Conditions The MagicPrep NGS is intended for indoor laboratory operation and storage only. M01534_v1.1 MagicPrep NGS Instrument Operating Manual Page 14 of 39...

  • Page 15: Emission And Immunity

    Operating altitude Max. 2000 m above sea level The MagicPrep NGS instrument has been designed to be able to operate under the above environmental conditions. However, with MagicPrep NGS reagent kits, due to the presence of temperature sensitive enzymes and specific temperature dependent reactions during the NGS library preparation workflow, narrower operating conditions are required.

  • Page 16: Description Of Function

    PCR using a thermal cycler. The operator is not able to access the internal parts of the MagicPrep NGS at any time. Insertion and removal of the reagent cartridge and sample deck are the only manual interactions with the instrument.

  • Page 17: Sample Deck

    The reagent cartridges should be stored in a freezer at ≤ -20 °C until required for use. The MagicPrep NGS instrument reads the 2D bar code on the upper surface of the reagent cartridge, which determines the specific library preparation protocol to be employed.
  • Page 18 Wear a lab coat, disposable gloves and protective glasses where necessary. Changing gloves between handling samples is recommended to avoid cross-contamination between samples or reagents. M01534_v1.1 MagicPrep NGS Instrument Operating Manual Page 18 of 39...

  • Page 19: Installation

     The MagicPrep NGS instrument has been designed to be operator installable. The MagicPrep NGS instrument weighs 18.8 kg and should be lifted by two people  On receipt of the shipping package, cut the black or white plastic straps securing the MagicPrep NGS shipping package to the wooden palette.

  • Page 20: Installation

    The foam packaging supports are designed to protect the MagicPrep NGS instrument.  Using two people, lift the MagicPrep NGS box up, remove the lower shipping cardboard tray to one side and remove the protective foam supports from each end. ...
  • Page 21 Internal Condensation The MagicPrep NGS has an evaporation tray within the instrument to collect any moisture that condenses from the internal refrigeration unit, which then evaporates. Operation of the MagicPrep NGS under extreme high humidity conditions could...

  • Page 22: Operation

    • Revelo mRNA-Seq for MagicPrep NGS User Guide (Doc. ID: M01535) • Revelo DNA-Seq Enz for MagicPrep NGS User Guide (Doc. ID: M01536) • Revelo DNA-Seq Mech for MagicPrep NGS User Guide (Doc. ID: M01537) Loading the Sample Deck and Reagent Cartridge Load the samples and magnetic beads into row 1 of the PCR tubes in the tube carrier/sample deck as described in the Reagent Kit user guide.
  • Page 23 When the front door is open, there is no power to any of the moving parts within the MagicPrep NGS instrument, however, there are instrument components that could potentially pinch or catch on a hand when inserted into the instrument...
  • Page 24 Load the sample deck assembly into the lower slot within the MagicPrep NGS door opening. The door has a cutout area to ease the alignment of the sample deck with the relevant opening.

  • Page 25: Starting A Library Preparation Run

    Operating Manual Starting a library preparation run Once the sample deck and reagent cartridge have been inserted into the MagicPrep NGS instrument, the door closed and the “Start” button pressed, follow the on-screen instructions. For detailed instructions on the next steps, refer to the reagent kit user guide(s) as outlined in section...

  • Page 26: Library Retrieval

    Library Retrieval On completion of the library preparation workflow, the MagicPrep NGS instrument will store the library preparation samples. The maximum time allowed between competition of library preparation and retrieval is 65 hours at typical laboratory temperatures (20 to 24°C ± 2°C and atmospheric pressure (1013 hPa ±...
  • Page 27  Insert the USB drive into the USB port on the front of the MagicPrep NGS instrument The USB port is only intended for installing software upgrades or extracting log files from or onto a USB storage device. Connection of any other USB devices such as a mouse or keyboard to the system is not allowed and access is blocked.

  • Page 28: Instrument Log

    Insert a USB drive into the USB port on the front of the instrument.  The MagicPrep NGS instrument will download the available log files to the USB drive. A white circle gives an indication of the progress M01534_v1.1 MagicPrep NGS Instrument Operating Manual...

  • Page 29: Errors

    Tecan support and email them as an attachment to Tecan.  Note: the log files are encrypted and can only be read by Tecan. 6.5.3 Errors  The MagicPrep NGS instrument will display error messages on the screen if...
  • Page 30  Switch off the instrument  Switch on the instrument to initiate the self- test/diagnostic sequence.  Reload the MagicPrep NGS and try to repeat the library preparation. If the error still exists, please contact Tecan Customer Support. Sample Deck &...
  • Page 31  Switch on the instrument to initiate the self- An error has occurred during the barcode test/diagnostic sequence.  Reload the MagicPrep NGS and try to reading process. repeat the library preparation. If the error still exists, please contact Tecan Customer Support.
  • Page 32  Switch off the instrument  Switch on the instrument to initiate the self- test/diagnostic sequence.  Reload the MagicPrep NGS and try to repeat the library preparation. If the error still exists, please contact Tecan Customer Support. Instrument motor...
  • Page 33  Switch off the instrument  Switch on the instrument to initiate the self- test/diagnostic sequence.  Reload the MagicPrep NGS and try to repeat the library preparation. If the error still exists, please contact Tecan Customer Support. Instrument homing...

  • Page 34: Magicprep Ngs Instrument Functional Test

    MagicPrep NGS system.  When setting up the MagicPrep NGS system for the first time, it is useful to perform a functional test in addition to the hardware test to confirm performance and set a baseline for your system. ...

  • Page 35: Support

    Tecan Genomics support. Press Continue to return to the Settings screen For help with any of our products, please contact Tecan Genomics Technical Support at 650.590.3674 (direct) or 888.654.6544, option 2 (toll-free, U.S. only) or email techserv-gn@tecan.com.

  • Page 36: System Care

    Decontamination, according to standard laboratory regulations is required under circumstances listed in section - Decontamination Declaration’ Substance residues on the MagicPrep NGS can cause personal injury and affect the integrity of the library preparation workflow. Decontaminate the MagicPrep NGS instrument before any interaction.

  • Page 37: Packaging For Return To Tecan

    Take care that the cardboard cover fits inside the white cardboard tray.  Using two people, lift one end of the MagicPrep NGS box up and slide one of the protective foam supports onto one end. Repeat this procedure at the other end of the packaging.
  • Page 38 Tighten the straps such that the pallet and package are not able to move.  Attach the return shipping label to the outside of the package and arrange for the shipping agent to collect the systems. M01534_v1.1 MagicPrep NGS Instrument Operating Manual Page 38 of 39...

  • Page 39: Disposal

    Products requires the marking for the restriction of the use of hazardous substances in electronic and electrical products. In accordance with the requirements specified in SJ/T11364‑2014, all electronic and electrical Tecan products sold in the People’s Republic of China are labelled with a marking for the restriction of the use of hazardous substances.

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