tecan Infinite 200 PRO Instructions For Use Manual page 132

5. Operating the Instrument
Example 4: Measurement of Ca
Plate
Part of the plate
Well
Kinetic Cycle
Kinetic condition
Injection
Fluorescence
Intensity (1)
Fluorescence
intensity (2)
Move Plate
132
2+
sensitive probes – Indo-1
Select an appropriate plate type. For fluorescence measurements, black
microplates are recommended.
For this example, a black 96 well plate was selected.
(Not shown); can be optionally selected if only part of the plate should be
processed
Mandatory for measurements with 'injection'
Select the number of necessary cycles
This strip allows actions to be performed once in a kinetic run at a certain
cycle. The intended injection strip below it is only processed once at the
selected cycle.
Injector A injects 20 µl with speed 200 µl/sec., refill mode: not selectable;
injection is performed at cycle 5 (defined by kinetic condition strip)
Select the appropriate parameters for the first label: Excitation wavelength:
340 nm, Emission wavelength: 410 nm; number of flashes: 25; integration
time: 40; gain: manual
Select the appropriate parameters for the second label: Excitation
wavelength: 340 nm, Emission wavelength: 480 nm; number of flashes: 25;
integration time: 40; gain: manual
Plate is moved out after finishing all wells
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04