I-Control Examples - tecan Infinite 200 PRO Instructions For Use Manual

5.14

i-control Examples

Example 1: Dual-Luciferase
Plate
Part of the plate
Well
Injection (1)
Wait (Timer)
Luminescence (1)
Injection (2)
Wait (Timer)
Luminescence (2)
Move Plate
STOP
2016-04
®
Assay (Promega Corp.)
For assay details please refer to www.promega.com.
Select an appropriate plate type. For luminescence measurements,
white microplates are recommended. For this example, a white 96-well
plate was selected.
(Not shown); can be optionally selected if only part of the plate is to be
processed.
Mandatory for measurements with 'injection'
Injector A injects 100 µl with speed 200 µl/sec., refill mode: standard
2 s wait time
Luminescence measurement with 10 s integration time, attenuation
'none'
Injector B injects 100 µl with speed 200 µl/sec., refill mode standard
2 s wait time
Luminescence measurement with 10 s integration time, attenuation
'none'
Plate is moved out after finishing all wells
SWITCH ON THE INSTRUMENT AT LEAST 15 MINUTES BEFORE
STARTING A LUMINESCENCE MEASUREMENT. SOME
COMPONENTS NEED TO WARM UP TO GUARANTEE STABLE
CONDITIONS FOR THE MEASUREMENT.
IFU for Infinite 200 PRO No. 30052730
5. Operating the Instrument
CAUTION
Rev. No. 1.6 129