tecan infinite 200 Instructions For Use Manual
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tecan infinite 200 Instructions For Use Manual

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TECAN
Instructions for Use for
®
infinite
200
Document Part No.: 30017581
2008-07
Document Revision No.: 1.4

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  • Page 1 TECAN Instructions for Use for ® infinite Document Part No.: 30017581 2008-07 Document Revision No.: 1.4...
  • Page 2 CAREFULLY READ AND FOLLOW THE INSTRUCTIONS PROVIDED IN THIS DOCUMENT BEFORE OPERATING THE INSTRUMENT. Notice Every effort has been made to avoid errors in text and diagrams; however, Tecan Austria GmbH assumes no responsibility for any errors, which may appear in this publication.
  • Page 3 ® ® • Tecan and the Tecan Logo are i-Control magellan infinite registered trademarks of Tecan Group Ltd., Männedorf, Switzerland ® ® • Windows and Excel are registered trademarks of Microsoft Corporation, Redmond, WA, USA Warnings, Cautions, and Notes...
  • Page 4 WARNING THIS SYMBOL INDICATES THE POSSIBLE PRESENCE OF BIOLOGICALLY HAZARDOUS MATERIAL. PROPER LABORATORY SAFETY PRECAUTIONS MUST BE OBSERVED. WARNING THIS SYMBOL INDICATES THE POSSIBLE PRESENCE OF FLAMMABLE MATERIALS AND A RISK OF FIRE. PROPER LABORATORY SAFETY PRECAUTIONS MUST BE OBSERVED. ATTENTION NEGATIVE ENVIRONMENTAL IMPACTS ASSOCIATED WITH THE TREATMENT OF WASTE.

  • Page 5: Table Of Contents

    Table of Contents 1. Safety .................... 9 Instrument Safety ..............9 2. General Description..............11 Instrument ................11 2.1.1 Intended Use .................11 2.1.2 Multifunctionality ..............12 2.1.3 Performance ................13 2.1.4 User Friendliness..............13 2.1.5 System Requirements ............14 Measurement Techniques.............15 2.2.1 Fluorescence .................15 2.2.2 Absorbance ................17 2.2.3 Luminescence................18 Injectors..................20...
  • Page 6 4.2.2 Filter Types ................56 4.2.3 Position of Polarization Filters..........57 Installing a Custom Filter ............58 4.3.1 Removing a Filter..............58 4.3.2 Mounting a Custom Filter ............58 Defining the Filters ..............60 5. Optical System ................65 ® Fluorescence Intensity System ― infinite M200....65 5.1.1 Light Source System Fluorescence Intensity ......67 5.1.2...
  • Page 7 6.3.1 Instrument Parameters ............96 6.3.2 FI Ratio Mode ................98 6.3.3 Measurement Accessories ............99 FP Measurements..............102 6.4.1 Fluorescence Polarization ...........102 6.4.2 Measurement Blank Range ..........102 6.4.3 G-Factor Settings..............103 6.4.4 Measurement with an Uncalibrated G-Factor ...... 103 6.4.5 Measurement with a Simultaneous G-Factor Calibration..104 6.4.6 Measurement with a Calibrated G-Factor ......
  • Page 8 Disinfection Certificate............151 Disposal................152 9.5.1 Disposal of Packing Material..........153 9.5.2 Disposal of Operating Material..........153 9.5.3 Disposal of the Instrument ...........154 10. Troubleshooting............... 155 Index....................159 Tecan Customer Support .............. 161 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07...

  • Page 9: Safety

    Caution STOP Tecan Austria GmbH have taken great care when creating the stored Plate Definition Files that are received with the instrument software. We take every precaution to ensure that the plate heights and well depths are correct according to the defined plate type. This parameter is used to determine the minimum distance between the top of the plate and the ceiling of the measurement chamber.
  • Page 10 1. Safety It is assumed that the instrument operators, because of their vocational experience, are familiar with the necessary safety precautions for handling chemicals and biohazardous substances. Adhere to the following laws and guidelines: National industrial protection law Accident prevention regulations Safety data sheets of the reagent manufacturers WARNING ®...

  • Page 11: General Description

    2. General Description 2. General Description 2.1 Instrument ® The Tecan is a multifunctional microplate reader with injector option. infinite ® provides high performance for the vast majority of today’s infinite microplate applications and research and is robotic compatible. 2.1.1 Intended Use ®...

  • Page 12: Multifunctionality

    2. General Description 2.1.2 Multifunctionality ® Depending on the type of wavelengths selection, the infinite 200 is available in two different versions: ® • M200 (monochromator-based instrument) infinite ® • F200 (filter-based instrument) infinite ® The following measurement techniques are supported by the infinite M200: •...

  • Page 13: Performance

    2. General Description 2.1.3 Performance ® has been designed to meet the requirements of a general- infinite purpose laboratory instrument. ® provides a range of parameters for optimizing the measurement infinite results according to: the assay type (cell-based or homogeneous), the microplate type, and the dispensed volumes per well and dispensing speeds.

  • Page 14: System Requirements

    Windows XP Professional (English), Minimum Service Pack 1 ® • Microsoft Excel 2000 (English) or above (for i-Control) ® Infinite 200 and i-control are also compatible with Windows Vista (32 Bit) and Excel 2007 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07...

  • Page 15: Measurement Techniques

    2. General Description 2.2 Measurement Techniques ® The following sections provide an introduction to the measurement infinite techniques when fully equipped. To keep this compact, a few simplifications have been made. For details see the references. 2.2.1 Fluorescence ® offers the basic fluorescence measurement technique and infinite some even more sophisticated variants: A.
  • Page 16 2. General Description B) Fluorescence Resonance Energy Transfer (FRET) Some microplate applications utilize a sophisticated dual labeling strategy. The FRET effect enables you to measure how many of two differently labeled compounds are in close proximity. This makes it suitable for binding studies. Basically, FRET is a fluorescence intensity measurement of one of the two fluorescent labels (acceptor).

  • Page 17: Absorbance

    2. General Description D) Fluorescence Polarization (FP) Fluorescence Polarization (FP) measures rotational mobility of a fluorescent labeled compound. FP is therefore particular suitable for binding studies, because the tumbling motion of small molecules may be dramatically slowed down after binding to a larger molecule. Fluorescence polarization measurements are based on the detection of the depolarization of fluorescence emission after excitation of a fluorescent molecule by polarized light.

  • Page 18: Luminescence

    2. General Description 2.2.3 Luminescence Caution STOP Switch on the instrument at least 15 minutes before starting a luminescence measurement. Some components need to warm up to guarantee stable conditions for the measurement. Glow Type Chemi- or Bioluminescence ® provides measurement of glow type chemi- or bioluminescence. infinite Glow type means that the luminescence assay glows much longer than a minute.
  • Page 19 2. General Description Flash Type Luminescence with Injectors Flash type luminescence is one of the measurement modes that can be performed with injectors. Note The plate detection sensor is only active if one of the injectors is in use (strips “injection” or “dispense”). Note During luminescence measurements it is important to close the lid covering the syringes and bottles of the reagent system to minimize...

  • Page 20: Injectors

    (XE-1000, Tecan Systems) located in a separate box, which feed one or two injector needles. The injector needles are designed to inject liquid in any SBS-conform microplate well types, in which the well-size is equal to or larger than an SBS standard 384-well plate.

  • Page 21: Measurement With Injectors

    2. General Description 2.3.1 Measurement with Injectors ® The injectors of the can be used with the following measurement infinite modes: Fluorescence Intensity top and bottom, Time Resolved Fluorescence, Absorbance, Flash and Glow Type Luminescence and Dual Color Luminescence. As the measurement position is not the same as the injector position, a short time delay (approx.

  • Page 22: Injector/Injector Carrier

    2. General Description 2.3.3 Injector/Injector Carrier The carrier, which includes the injector needles, can be easily removed from the instrument for priming or washing the system and for optimizing the injection speed. Figure 2-3: Injector carrier When using the injector during a measurement or for just dispensing a plate the injector carrier must be inserted correctly into the instrument.
  • Page 23 2. General Description Picture 2-1: Inserting the injector carrier into the injector port Caution The injector carrier must be in the service position STOP for washing und priming. Prime and Wash must not be performed when the injector is in the instrument! Caution If the injector carrier is not inserted correctly in the injector port, the STOP...
  • Page 24 2. General Description The dead volume of the injection system (injector needles, syringes, valves and tubing) is approximately 100 µl after ‘backflush’ for each syringe. The function of backflush is to return any unused reagent to the reservoir bottles. The injection speed can be adjusted via the software to allow for good mixing of reagents.

  • Page 25: Priming And Washing Of The Infinite

    2. General Description ® 2.3.4 Priming and Washing of the infinite Caution The injector carrier must be in the service position STOP for washing und priming. Prime and Wash must not be performed when the injector is in the instrument! The initial filling step of the injector system (priming) as well as the cleaning step of the injector system (washing) must take place outside of the instrument.
  • Page 26 2. General Description Caution A prime volume below 2 ml in an empty system may result in incomplete STOP filling of the system, and therefore may negatively affect assay performance. Service-Position Figure 2-5 ‘Service Position’ of the injectors. The injectors are removed from the carrier slot and inserted into the holder of the injector carrier system.
  • Page 27 2. General Description Priming Before the injection system can be used, an initial filling step (priming) is needed to remove all air and to completely fill the system with liquid. It is recommended to perform a washing step before priming. Priming can be performed by using the i-Control software or by using the hardware buttons on the injector box: Priming procedure (general):...
  • Page 28 2. General Description Priming Procedure (hardware button): Priming can also be performed without using the software. Priming parameters can be stored on the injector by clicking Save as Default on the Prime tab of the Injector Maintenance dialog box of the i-Control software (in the Settings menu, click Injectors...
  • Page 29 2. General Description Reagent Backflush Reagent backflush allows reagents in the tubing system to be pumped back into storage bottles. This action can be performed optionally prior to washing the injector system to minimize the dead volume. Reagent backflush procedure: Remove the injector carrier from the instrument and insert the injector carrier into the service position of the injector box.
  • Page 30 2. General Description Caution The injector carrier must be in the service position STOP for the action ‘backflush’. Do not perform backflush when the injector is in the instrument! Washing Before the instrument is switched off, it is recommended to perform a wash procedure to clean the injector system.
  • Page 31 2. General Description Washing (i-Control): Adjust the parameters on the Wash tab of the Injector Maintenance dialog box in the Settings menu Start the washing procedure by clicking the Start wash. Select one of the injectors Injector A or Injector B or Wash Injector A and B.
  • Page 32 2. General Description Washing (hardware buttons): Washing can also be performed without using the software. Washing parameters can be stored on the injector by clicking Save as Default on the Wash tab of the Injector Maintenance dialog box (in the Settings menu, click Injectors... and the Injector Maintenance dialog box appears) of the i-Control software.
  • Page 33 2. General Description Waste Tub When starting a measurement with the actions ‘injection’ or ‘dispense’, 5 µl of liquid are dispensed into a disposable container on the plate carrier before starting ‘injection’ or ‘dispense’. This initial dispense step makes sure that the injection/dispense conditions are equal for each well.
  • Page 34 2. General Description Waste tub: Click the ‘Empty Waste tub’ button and the plate carrier will move out automatically. Remove the waste tub and empty the contents. After the waste tub has been emptied place it back on the plate carrier. The i-Control software will alert you when the waste tub needs to be emptied again.

  • Page 35: Injector Cleaning And Maintenance

    2. General Description 2.3.5 Injector Cleaning and Maintenance The required maintenance may vary with your application. The following procedures are recommended for optimal performance and maximum life of the injector system. Daily Maintenance: If not otherwise stated by the manufacturer of the kit to be used, the following tasks must be performed at least daily: •...

  • Page 36: Injector Reagent Compatibility

    2. General Description 2.3.6 Injector Reagent Compatibility ® The injector system of the infinite F/M200 consist of the following materials: • Teflon (PTFE): Tubing, valve plug, seal • KelF: Valve body • SC05: Injector needles Please refer to the following list for reagent compatibility. The column ‘Rating’ indicates the compatibility with the reagents listed in the ‘Chemical’...
  • Page 37 2. General Description Caution The information in this table has been supplied to Tecan Austria by other reputable sources and is to be used ONLY as a guide in selecting STOP equipment for appropriate chemical compatibility. Before permanent installation, test the equipment with the chemicals and under the specific conditions of your application.

  • Page 38: Software

    For advanced data reduction and full regulatory compliance with CFR 2 part 11 ® guidelines, The Magellan software can be used to control the . (For infinite more information, contact your local Tecan representative). 2.4.1 i-Control and Injectors When using the injector, two modes are available: •...
  • Page 39 2. General Description Using the Dispense Strip: Plate Select an appropriate plate type Optional; Part of the plate Select the wells to be dispensed Dispense Set up the dispense parameters. If both injectors are selected, all wells are first dispensed with injector A and then with injector B.
  • Page 40 2. General Description Injection Mode The injection settings can be adjusted via the software: Injection Select Injector: Injector A or Injector B can be selected. It is not possible to select both injectors on one strip. If a measurement with two injectors is to be performed, two injector strips are necessary.
  • Page 41 2. General Description Using the Injection Strip: Plate Select an appropriate plate type. Part of the plate Optional; Select the wells to be dispensed Well The well strip is mandatory. Injection is only possible with a ‘well’ strip. This strip ensures that the following indented strips are performed well-wise.
  • Page 42 2. General Description Wait Strip A Wait time (delay or settle time) can be inserted into the procedure. Select a time in hh:mm:ss from 00:00:01 up to 23:59:59 Wait time Options If ‘Wait for injection’ is selected, the wait time includes the injection time.

  • Page 43: I-Control Examples

    2. General Description 2.4.2 i-Control Examples Example 1: Dual Luciferase Assay™ (Promega Corp.) For assay details please refer to www.promega.com. Plate Select an appropriate plate type. For luminescence measurements, white microplates are recommended. For this example, a white 96-well plate was selected.
  • Page 44 2. General Description ® Example 2: Enliten ATP Assay System Bioluminescence Detection Kit for ATP (Promega Corp.) For assay details please refer to www.promega.com. Plate Select an appropriate plate type. For luminescence measurements, white microplates are recommended. For this example, a white 96 well plate was selected. Part of the plate (Not shown);...
  • Page 45 2. General Description Example 3: Measurement of Ca sensitive probes – Fura-2 Plate Select an appropriate plate type. For fluorescence measurements, black microplates are recommended. For this example, a black 96 well plate was selected. Part of the plate (Not shown), can be optionally selected if only part of the plate should be processed Well Mandatory for measurements with ‘injection’...
  • Page 46 2. General Description Example 4: Measurement of Ca sensitive probes – Indo-1 Plate Select an appropriate plate type. For fluorescence measurements, black microplates are recommended. For this example, a black 96 well plate was selected. Part of the plate (Not shown); can be optionally selected if only part of the plate should be processed Well Mandatory for measurements with ‘injection’...

  • Page 47: Installation

    Caution The reader has been tested with the supplied USB cable. If another USB STOP cable is used, Tecan Austria cannot guarantee the correct performance of the instrument. 2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4...

  • Page 48: Unpacking Procedure

    3. Installation 3.1.1 Unpacking Procedure Visually inspect the container for damage before it is opened. Report any damage immediately. Select a location to place the instrument that is flat, level, vibration free, away from direct sunlight, and free from dust, solvents and acid vapors. Allow at least 10 cm distance between the back of the instrument and the wall or any other equipment.

  • Page 49: Removal Of The Transport Locks

    3. Installation 3.2 Removal of the Transport Locks Caution STOP Remove the transport lock before operating the instrument. The instrument is delivered with the plate carrier and filter carrier/cuvette carrier locked into place, so that they cannot be damaged. Before the instrument can be used, the transport locks must be removed using the following procedure: 1.
  • Page 50 3. Installation 4. Loosen the screw from other transport lock, which is only in the plate carrier compartment (right transport lock in picture above). 5. Pull the plate carrier out manually. 6. Remove the screw from the transport lock, which is only in the plate carrier compartment (right transport lock in picture above).

  • Page 51: Transport And Storage

    3. Installation 3.3 Transport and Storage 3.3.1 Transport ® must be shipped using the original packing and installed infinite transport locks. Before shipping the instrument, it must be thoroughly disinfected (see 9.3 Instrument Disinfection). 3.3.2 Storage Before storing the instrument the injectors must be rinsed using a wash procedure (see 2.3.4 Priming and Washing of the infinite®...

  • Page 52: Switching The Instrument On

    Caution The reader has been tested with the supplied USB cable. If another USB STOP cable is used, Tecan Austria cannot guarantee the correct performance of the instrument. Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07...
  • Page 53 Warranty Label USB Connection Injector Connection Caution STOP Only Tecan authorized service technicians are allowed to open the instrument. Removing or breaking the warranty seal voids the warranty. 2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4...

  • Page 55: Defining Filter Slides (Infinite ® F200 Only)

    The optical filters (bandpass style) in a filter slide are specially designed for fluorescence measurements. The spectral rejection and the bandwidth of the fluorescence filters are optimized for achieving excellent sensitivity. Contact TECAN for filters other than those supplied on the delivered filter slides. 4.1.2 Absorbance Filters Bandpass filters, which are commonly used in microplate readers for absorbance measurements, usually have a bandwidth of 10 nm.

  • Page 56: Filter Types

    4. Defining Filter Slides (infinite® F200 only) 4.2.2 Filter Types Caution There are two types of filters. It is important that light travels through STOP both types of filter in the correct direction. Before inserting a new filter carefully consider the filter and the direction of light through the filter slide.

  • Page 57: Position Of Polarization Filters

    4. Defining Filter Slides (infinite® F200 only) 4.2.3 Position of Polarization Filters Note ® Fluorescence polarization measurements on Infinite F200 require two identical excitation and emission filters placed together with the polarizers either on the positions 1 and 2 or 3 and 4. ®...

  • Page 58: Installing A Custom Filter

    4. Defining Filter Slides (infinite® F200 only) 4.3 Installing a Custom Filter When installing a new filter use the filter assembly tool included in the accessories case. For installing the polarizers use the soft tweezers (plastic). 4.3.1 Removing a Filter 1.
  • Page 59 4. Defining Filter Slides (infinite® F200 only) Caution The filters are precision optical components, which should be handled STOP by the edges and not scratched or stored face down in a drawer. Once the filters are installed in the slide, they are relatively well protected, but care should be exercised when handling or storing them.

  • Page 60: Defining The Filters

    4. Defining Filter Slides (infinite® F200 only) 4.4 Defining the Filters Caution Any changes to the filters in the filter slide are to be carried out by trained personnel! The instrument is able to recognize predefined filter slides and you should not attempt to change the filter values. However, if the filters in the filter slide have been changed (by a service STOP engineer) or if a new undefined customized filter slide is to be used, the...
  • Page 61 4. Defining Filter Slides (infinite® F200 only) Note No special characters (blank, ?, $, %, ., /, etc.) except '_' are allowed for the filter slide description. Caution The filter slide description is part of the G-Factor key value. STOP If manually entered, avoid using the same description for the different filter slides.
  • Page 62 4. Defining Filter Slides (infinite® F200 only) Note Fluorescence polarization mode on Position 1 requires the same filter settings on Position 2 and vice versa. Fluorescence polarization mode on Position 3 requires the same filter settings on Position 4 and vice versa.
  • Page 63 4. Defining Filter Slides (infinite® F200 only) Flash Counter: The flash counter monitors the number of flashes through a filter. The flash counter number provides the user only with additional information about the filter in use. The flash counter number is saved together with other information about the filter on the filter slide microchip.

  • Page 65: Optical System

    5. Optical System 5. Optical System ® 5.1 Fluorescence Intensity System ― infinite M200 The optical system of the fluorescence top and bottom system of the ® is sketched below. The system consists of the light source system infinite M200 (1) including the excitation double monochromator (2), the fluorescence top optics (3), the emission double monochromator (4) and the fluorescence detection (5).
  • Page 66 5. Optical System Figure 5-2: Optical System Fluorescence Bottom Figure 5-3: Detailed view of excitation and emission double monochromator unit Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07...

  • Page 67: Light Source System Fluorescence Intensity

    5. Optical System 5.1.1 Light Source System Fluorescence Intensity Fluorescence applications usually require a specific range of excitation wavelengths. Additionally, pulsed excitation light may be required (Time Resolved Fluorescence [TRF]). ® light source system is built from the following components: infinite M200 •...
  • Page 68 5. Optical System Description of how a Monochromator Works A monochromator is an optical instrument that enables any wavelength to be selected from a defined optical spectrum. Its method of operation can be compared to a tunable optical filter, which allows both the wavelength and bandwidth to be adjusted.

  • Page 69: Fluorescence Top/Bottom Optics

    5. Optical System 5.1.2 Fluorescence Top/Bottom Optics Flash light enters the optical system being focused by the condenser onto the entrance slit of the Excitation Monochromator. The wavelength of the excitation light is selected within the monochromator. After passing the monochromator, the excitation light is coupled into a fiber bundle guiding the light to the top or bottom measuring head.

  • Page 70: Fluorescence Intensity Detection

    5. Optical System 5.1.3 Fluorescence Intensity Detection The fluorescence detection system is used for both measuring modes: fluorescence from above (top) and below the microplate wells (bottom). The fluorescence light is focused onto the entrance slit of the Emission Monochromator. After passing the monochromator the light is focused onto the detector (PMT).

  • Page 71: Fluorescence Intensity System - Infinite

    5. Optical System ® 5.2 Fluorescence Intensity System ― infinite F200 The following parts constitute the fluorescence intensity system of the ® instrument: infinite F200 • Light Source (1) • Fluorescence Optics (2) • Fluorescence Detection System (3). The fluorescence top system is shown in Figure 5-4, the bottom system in Figure 5-5.
  • Page 72 5. Optical System ® Figure 5-5: Fluorescence intensity bottom system of the infinite F200 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07...

  • Page 73: Light Source System

    5. Optical System 5.2.1 Light Source System Flash light enters the optical system by being focused through an orifice containing the filter. This opening acts as a color specific light source. ® light source system is built from the following components: infinite F200 •...

  • Page 74: Fluorescence Optics Top

    5. Optical System 5.2.2 Fluorescence Optics Top Flash light enters the optical system by being focused through an orifice. This opening acts as a color specific light source. A semi-transparent mirror (beam splitter) reflects 50% of the light towards the microplate. The objective lens system focuses the light into the sample.

  • Page 75: Fluorescence Polarization System - Infinite ® F200

    5. Optical System 5.3 Fluorescence Polarization System ― ® infinite F200 For technical details please refer to chapter 5.2 Fluorescence Intensity System ― ® infinite F200. ® configured for Fluorescence Polarization (FP) measurements is infinite F200 delivered with a standard FP filter slide. The filter slide is equipped with filters and polarizers for excitation and emission, at 485 and 535 nm respectively, and can be applied for measuring, for example, fluorescein-based FP applications.

  • Page 76: Absorbance System - Infinite ® M200

    5. Optical System ® 5.4 Absorbance System ― infinite M200 For absorbance measurements, a similar optical path is used as for fluorescence excitation. For details of the light source (1) and the excitation monochromator (2), please refer to 5.1.1 Light Source System Fluorescence Intensity. A fiber bundle guides the light from the excitation monochromator to the absorbance microplate (MTP) optics (3), which focuses the light into the wells.

  • Page 77: Absorbance Optics Mtp

    (2µl) use Tecans’s NanoQuant Plate. With this device it is possible to measure 16 different samples within one measurement. For further information please contact your local Tecan distributor or visit: www.tecan.com 2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4...

  • Page 78: Cuvette Port

    5. Optical System ® 5.5 Cuvette Port (infinite M200) ® may be optionally equipped with a cuvette port for infinite M200 absorbance measurements. ® Figure 5-7: Optical System of the absorbance module of infinite M200 including the cuvette port ® For absorbance measurements with the cuvette port of infinite M200 a similar optical path is used as for fluorescence excitation.

  • Page 79: Absorbance Optics Cuvette

    ® available for infinite F200. With the infinite F200, cuvettes may be measured using a Tecan Cuvette Adapter placed on the plate transport. 5.5.1 Absorbance Optics Cuvette A fiber bundle guides the light from the excitation monochromator system to the absorbance cuvette optics.

  • Page 80: Cuvette Types

    5. Optical System 5.5.3 Cuvette types The cuvette port is compatible with the following cuvettes: Maximum Height Cuvette Type Width x Depth Filling Volume Example (including lid) Hellma 110 QS, Standard cuvettes 12.5 x 12.5 mm 55 mm 2 ml ∗...

  • Page 81: Inserting The Cuvette

    5. Optical System 5.5.4 Inserting the Cuvette The cuvette holder is attached securely to the cuvette carrier and moves the cuvette in and out. The cuvette carrier is an integral part of the instrument and cannot be removed. Measureme nt Window ®...

  • Page 82: I-Control And The Cuvette Port

    5. Optical System 5.5.5 i-Control and the Cuvette Port Cuvette Strip For performing cuvette measurements, a ‘Cuvette’ strip is necessary (see Figure 5-10). Figure 5-10: Cuvette strip For a few applications it might be necessary to combine a microplate measurement with a cuvette measurement. The i-Control software therefore allows the usage of one cuvette strip and one plate strip within one measurement script.

  • Page 83: I-Control Cuvette Examples

    5. Optical System Caution STOP Blanking data will be overwritten without a warning message when starting another blanking measurement. Blanking data will be deleted without a warning message when closing the i-Control software. 5.5.6 i-Control Cuvette Examples Example 1: Example of how to use the ‘Blanking’ measurement when measuring a DNA sample: 1) Prepare cuvette with sample buffer 2) Set up the DNA measurement in the i-Control software:...
  • Page 84 5. Optical System 4) The instrument is initialized and the cuvette holder moves out. The user is requested to insert the blank cuvette: Insert the blank cuvette and click OK to start the blank measurement. The ® measured blank data are displayed in an Excel spreadsheet.
  • Page 85 5. Optical System Example 2: Combination of microplate and cuvette measurement: For some applications it might be necessary to compare data measured on a microplate with cuvette data. The following example shows how to set up this measurement in general: Necessary for cuvette measurements.
  • Page 86 5. Optical System Example 3: Usage of ‘Move Cuvette OUT’ strip when measuring a combination of microplate and cuvette: Cuvette Necessary for cuvette measurement Absorbance strip Up to 4 absorbance fixed wavelength strips are allowed. Reference wavelength is only selectable when using one (cuvette) absorbance fixed wavelength strip.

  • Page 87: Absorbance System - Infinite ® F200

    5. Optical System ® 5.6 Absorbance System ― infinite F200 For absorbance measurements a similar optical path is used as for fluorescence excitation. The absorbance measurement module is located underneath the plate carrier. It measures the light being transmitted through the sample. Before measurement of the microplate, a reference measurement is performed with the plate carrier moved out of the light beam (see also 2.2.2 Absorbance).

  • Page 88: Light Source System

    (2µl) use Tecans’s NanoQuant Plate With this device it is possible to measure 16 different samples in one measurement. For further information please contact your local Tecan distributor or visit: www.tecan.com Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07...

  • Page 89: Luminescence System

    5. Optical System 5.7 Luminescence System ® Luminescence System consists of the following parts: infinite Luminescence Optics Detection Unit (Basic or Standard PMT) Figure 5-13: Optical System Luminescence The luminescence fiber bundle guides the light from the sample to the detection unit (PMT) passing a filter wheel.

  • Page 90: Luminescence Optics

    5. Optical System 5.7.1 Luminescence Optics ® In luminescence measurement mode, the uses fixed microplate infinite position and a moveable luminescence measurement head (see Figure 5-13: Optical System Luminescence). The plate thickness is defined by selecting the corresponding plate type in the software (see i-Control Instructions for Use). Fiber A glass fiber guides the light from the sample to the detection unit.
  • Page 91 5. Optical System Figure 5-15: Transmission spectrum of filter ‘Lumi Green’ Wavelength [nm] Figure 5-16: Transmission spectrum of filter ‘Blue 1’ Wavelength [nm] Figure 5-17: Transmission spectrum of filter ‘Green 1’ 2008-07 Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4...

  • Page 92: Luminescence Detection

    5. Optical System 5.7.2 Luminescence Detection Caution STOP Switch on the instrument at least 15 minutes before starting a luminescence measurement. Some components need to warm up to guarantee stable conditions for the measurement. ® luminescence detection system utilizes the single photon infinite counting measurement technique.

  • Page 93: Operating The Infinite ® 200

    6. Operating the infinite® 200 ® 6. Operating the infinite 6.1 Introduction ® is operated using a personal computer based software control. infinite i-Control or Magellan software may be used as the user interface. For details see the corresponding software Instructions for Use. This short introduction is for a general understanding of instrument parameters and operation.

  • Page 94: General Operating Features

    6. Operating the infinite® 200 6.2 General Operating Features ® has some general behavior and options, which are independent infinite from a particularly selected measurement technique. 6.2.1 Instrument Start Up Before the instrument is switched ON, check if the USB interface cable is connected.

  • Page 95: General Options

    6. Operating the infinite® 200 6.2.3 General Options The following options may be taken independently from the particular measurement technique. Note To keep temperature on a constant level and provide uniformity across the plate, the plate must be placed in “incubation position”. When the “heating”...

  • Page 96: Optimize Fluorescence Measurements

    255. Gain settings below 60 are possible and might be useful for special applications, but the performance of the PMT is not specified for voltage supply < 300 V. Tecan therefore does not take responsibility for measurement results of ®...
  • Page 97 6. Operating the infinite® 200 Where U is the voltage, Gain is the selected gain setting, 255 is the maximum possible gain and 1250 V is the maximum voltage supply of the PMT. Example: A gain of 100 corresponds to a voltage supply of 490 V: 1250 Flash Settings On the fly measurements with 1 flash (read) per well are possible for all plate...

  • Page 98: Fi Ratio Mode

    6. Operating the infinite® 200 6.3.2 FI Ratio Mode Ratio Mode Up to 4 labels may be measured well-wise. This measurement mode is called ‘ratio mode’. Be aware that no ‘ratio’ calculation is performed after this ® measurement. The Excel result sheet shows the raw data.

  • Page 99: Measurement Accessories

    6. Operating the infinite® 200 6.3.3 Measurement Accessories ® Recommended Filters (infinite F200 Only) Please ask your local Tecan dealer for a recommended filter set. Filters designed for a different type of instrument will not necessarily perform well with the ® infinite F200. Note...
  • Page 100 6. Operating the infinite® 200 Plate Definition File Catalog Number Manufacturer (*.pdfx) 650 101 650 161 Greiner Bio-One, GRE96ut 650 160 www.gbo.com/bioscience 650 180 650 185 651 101 651 161 Greiner Bio-One, GRE96vt www.gbo.com/bioscience 651 160 651 180 781 079 781 086 781 077 (Fluotrac 600) Greiner Bio-One,...
  • Page 101 6. Operating the infinite® 200 Plate Definition File Catalog Number Manufacturer (*.pdfx) 3680 (Non-Treated) 3700 (Treatment: High) Corning, COS384ft www.corning.com/lifesciences/ 3701 (TC-Treated) 3702 (Non-Treated) 3703 (Treatment: High) Corning, COS384fw 3704 (TC-Treated) www.corning.com/lifesciences/ 3705 (non-Treated) COS384fb_assay plate clear Corning, 3711 (Non-Treated) www.corning.com/lifesciences/ bottom 3712 (TC-Treated)

  • Page 102: Fp Measurements

    6. Operating the infinite® 200 6.4 FP Measurements 6.4.1 Fluorescence Polarization Fluorescence Polarization (FP, P) is defined by the following equation: − ⊥ ⊥ where I and I equal the emission intensity of the polarized light parallel and ⎟⎟ ⊥ perpendicular to the plane of excitation, respectively.

  • Page 103: G-Factor Settings

    6. Operating the infinite® 200 6.4.3 G-Factor Settings The given equation for calculation of fluorescence polarization assumes that the sensitivity of the detection system is equivalent for parallel and perpendicular polarized light. This is generally not the case and either the parallel or perpendicular intensity must be corrected by so called ‘G-Factor’.

  • Page 104: Measurement With A Simultaneous G-Factor Calibration

    6. Operating the infinite® 200 6.4.5 Measurement with a Simultaneous G-Factor Calibration When Calibrate is selected, the G-factor is determined for the current measurement parameters and used for the following FP measurement. In order to perform the G-Factor calibration, please define: •...

  • Page 105: Measurement With A Calibrated G-Factor

    6. Operating the infinite® 200 Caution STOP The filter slide description is part of the G-Factor key value. Avoid using the same filter slide description for different filter slides as this will affect the correct G-Factor recognition. 6.4.6 Measurement with a Calibrated G-Factor Note Once calibrated, the G-factor is shown and can be used immediately if it matches the Ex/Em wavelength pair and the filter slide description.

  • Page 106: Measurement With A Manual G-Factor

    6. Operating the infinite® 200 6.4.7 Measurement with a Manual G-Factor If the displayed G-Factor does not match the calibrated value (e.g. the G-Factor has been manually changed or loaded with a method), the corresponding value will be marked as ‘Manual G-Factor’. The calibrated G-Factor can be restored by clicking the ‘>>’...

  • Page 107: Calculation Of Fluorescence Polarization Parameters

    6. Operating the infinite® 200 6.4.8 Calculation of Fluorescence Polarization Parameters G-Factor: cross cross − − − Polarizati value reference Averaged relative fluorescen units reference Averaged relative fluorescen units buffer Blank Reduction: The mean value of the respective blank wells is subtracted from each value. ⎧...

  • Page 108: Optimize Absorbance Measurements

    6. Operating the infinite® 200 6.5 Optimize Absorbance Measurements 6.5.1 Measurement Parameters Flash Settings On the fly measurements with 1 flash (read) per well are possible for all plate types; however, measurement precision at low light levels depends on the reading time during which a fluorescence signal can be received.

  • Page 109: Multiple Reads Per Well

    6. Operating the infinite® 200 6.6 Multiple Reads Per Well The i-Control software allows multiple reads per well (MRW) to be performed in absorbance, fluorescence top and fluorescence bottom mode. The multiple reads per well functions can be activated on an absorbance or fluorescence intensity program strip by selecting the ‘Multiple reads per well’...

  • Page 110: Mrw Size

    6. Operating the infinite® 200 Pattern examples: Square: Square (filled): Circle: Circle (filled): X-line: Y-line: XY-line: 6.6.2 MRW Size The MRW size determines the number of points to be measured in a well. ® ® Depending on the microplate type and instrument ( infinite F200 infinite...
  • Page 111 6. Operating the infinite® 200 In addition, the plate type (e.g. material of bottom of the microplate) also influences the characteristics of the light beam. Therefore the theoretical border displayed in the software might not correspond to the actual border when measuring a real sample.

  • Page 112: Result Display In Ms Excel

    6. Operating the infinite® 200 ® 6.6.4 Result Display in MS Excel ® The MS Excel result sheet generated by the i-Control software displays a schematic graphical overview (‘Multiple Reads Per Well – Alignment’; see Figure 6-2) of the measurement points. A number is assigned to each measurement point.

  • Page 113: Optimize Luminescence Measurements

    6. Operating the infinite® 200 6.7 Optimize Luminescence Measurements Caution STOP Switch on the instrument at least 15 minutes before starting a luminescence measurement. Some components need to warm up to guarantee stable conditions for the measurement. 6.7.1 Integration Time At very low light levels, a PMT does not yield a continuous output current, which is necessary for a reliable analog to digital conversion.

  • Page 115: Instrument Features

    7. Instrument Features 7. Instrument Features 7.1 Introduction Note All specifications are subject to change without prior notification. The following types of measurement are provided with the fully equipped ® microplate reader: infinite Measurement Type Description Fluorescence Intensity Top/Bottom See 7.3 Fluorescence Intensity and Time Resolved Fluorescence (TRF) Fluorescence Time Resolved See 7.3 Fluorescence Intensity and...

  • Page 116: Instrument Specifications

    7. Instrument Features 7.2 Instrument Specifications The table below lists the technical specifications of the instrument: Parameters Characteristics Measurement Software controlled Interface Filter Handling External filter exchange ® infinite F200 Filter Handling Monochromator-based wavelengths change – no filters ® necessary infinite M200 Microplates to be...

  • Page 117: Fluorescence Intensity And Time Resolved Fluorescence (Trf)

    7. Instrument Features 7.3 Fluorescence Intensity and Time Resolved Fluorescence (TRF) Parameters Characteristics Wavelength Range - 230 - 850 nm ® infinite M200: selectable in 1 nm steps Wavelength Range - 230 - 850 nm ® infinite F200: Standard Filter: Not applicable –...

  • Page 118: Definition Of The Detection Limit

    7. Instrument Features 7.3.1 Definition of the Detection Limit The detection limit is the fluorophore concentration where the background- subtracted signal equals 3 times the standard deviation of the background noise. When selecting 1 flash per well, the plate carrier does not stop at the measurement position.

  • Page 119: Fluorescence Polarization (Fp- Infinite ® F200 Only)

    7. Instrument Features ® 7.4 Fluorescence Polarization (FP- infinite F200 only) Note The option ‘Fluorescence Polarization’ is only available for the ® ® F200. This module cannot be installed on a infinite infinite M200. Parameters Instrument type Characteristics Wavelength Range standard Excitation: 300 –...

  • Page 120: Absorbance

    7. Instrument Features 7.5 Absorbance Parameters Instrument Type Characteristics ® Wavelength Range 230 – 1000 nm infinite F200 ® 230 – 1000 nm infinite M200 no filter necessary, selection in 1 nm steps possible Measurement Range Both 0 – 3 OD The following specifications are valid for the wavelength range from ®...

  • Page 121: Glow Type Luminescence

    7. Instrument Features 7.6 Glow Type Luminescence Caution STOP Switch on the instrument at least 15 minutes before starting a luminescence measurement. Some components need to warm up to guarantee stable conditions for the measurement. Luminescence Detection uses the photon counting technique. Parameters Characteristics Wavelength Range...

  • Page 122: Flash Type Luminescence

    7. Instrument Features 7.7 Flash Type Luminescence Caution STOP Switch on the instrument at least 15 minutes before starting a luminescence measurement. Some components need to warm up to guarantee stable conditions for the measurement. Luminescence Detection uses the photon counting technique. Parameters Characteristics Wavelength Range...

  • Page 123: Dual Color Luminescence

    7. Instrument Features 7.8 Dual Color Luminescence (e.g. BRET Caution STOP Switch on the instrument at least 15 minutes before starting a luminescence measurement. Some components need to warm up to guarantee stable conditions for the measurement. Parameters Characteristics Built-in Wavelength: See 5.7 Luminescence System Integration Time: 1 - 20000 ms.

  • Page 124: Cuvette Measurements (Infinite ® M200 Only)

    7. Instrument Features ® 7.10 Cuvette Measurements (infinite M200 only) Note ® The option ‘Cuvette’ is only available for the infinite M200. This module ® cannot be installed on a infinite F200. The cuvette option allows performing absorbance measurement in fixed wavelength and scan mode.

  • Page 125: Injector

    7. Instrument Features 7.11 Injector Parameters Characteristics Accuracy < 10% for injection volume of 10 µl < 2% for injection volume of 100 µl < 0.7% for injection volume of 450 µl Precision < 10% for injection volume of 10 µl <...

  • Page 127: Quality Control

    8.1 Periodic Quality Control Tests Depending on usage and application, we recommend a periodic evaluation of the instrument at Tecan Austria. The tests described in the following sections do not replace a full evaluation by the manufacturer or authorized dealers. But the tests may be performed periodically by the user to check significant aspects of the instrument performance.

  • Page 128: Specifications - Passed/Failed Criteria

    8. Quality Control 8.2 Specifications - Passed/Failed Criteria Note All specifications are subject to change without prior notification. The following table gives an overview of the passed/failed criteria for the ® specification test of the infinite Specification Microplate Passed/Failed Criteria Fluorescence Top Sensitivity <...

  • Page 129: Specifications - Test Instructions

    8. Quality Control 8.3 Specifications - Test Instructions 8.3.1 Fluorescence Top ® For the with the option ‘Fluorescence Top’, the following tests can infinite be performed to prove the specifications: • Sensitivity • Uniformity • Precision ® These test instructions are valid for the infinite ®...
  • Page 130 8. Quality Control Plate Layout: Pipette 200 µl of 1 nm Fluorescein or the blank solution (0.01 M NaOH) into the appropriate wells according to the plate layout: <> Material/Reagents: 1 nm Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377) 0.01 M NaOH (=Blank) (NaOH pellets, Merck article no.
  • Page 131 8. Quality Control Uniformity Perform the following measurement to determine the Uniformity: Measurement Parameters: Parameter Setting Reading Mode Fluorescence Top ® Exc Wavelength : 485 (20 nm) infinite F200 ® : 485 nm infinite M200 ® Em Wavelength : 535 (25 nm) infinite F200 ®...
  • Page 132 8. Quality Control Precision Perform the following measurement to determine the precision/reproducibility: Measurement Parameters: Parameter Setting Reading Mode Fluorescence Top ® Exc Wavelength : 485 (20 nm) infinite F200 ® : 485 nm infinite M200 ® Em Wavelength : 535 (25 nm) infinite F200 ®...

  • Page 133: Fluorescence Bottom

    8. Quality Control 8.3.2 Fluorescence Bottom ® For the with the option ‘Fluorescence Bottom’ the following tests infinite may be performed to prove the specifications: • Sensitivity • Uniformity • Precision/Repeatability ® These test instructions are valid for the infinite ®...
  • Page 134 8. Quality Control Material/Reagents: 25 nm Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377) 0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045) 1 Greiner 96-well plate µClear, black with transparent bottom 200 µl Pipette + tips Calculation of Detection Limit (Sensitivity): See 8.3.1 Fluorescence Top: Sensitivity.
  • Page 135 8. Quality Control Precision Perform the following measurement to determine the precision/reproducibility: Measurement Parameters: Parameter Setting Reading Mode Fluorescence Bottom ® Exc Wavelength : 485 (20 nm) infinite F200 ® : 485 nm infinite M200 ® Em Wavelength : 535 (25 nm) infinite F200 ®...

  • Page 136: Time Resolved Fluorescence

    8. Quality Control 8.3.3 Time Resolved Fluorescence ® For the with the option ‘Fluorescence Top’ the following tests may infinite be performed to prove the specifications: • Sensitivity • Precision/Repeatability ® These test instructions are valid for the infinite ® •...
  • Page 137 8. Quality Control Material/Reagents: 1 nm Europium (B119-100, HVD Live Sciences) Enhancement Solution (=Blank) (1244-105, HVD Live Sciences) 1 Greiner 96-well plate white 200 µl Pipette + tips Calculation of Detection Limit (Sensitivity): See 8.3.1 Fluorescence Top: Sensitivity. Precision Perform the following measurement to determine the precision/reproducibility: Measurement Parameters: Parameter Setting...

  • Page 138: Fluorescence Polarization (Infinite ® F200 Only)

    8. Quality Control ® 8.3.4 Fluorescence Polarization (infinite F200 only) ® For the infinite F200 with the option ‘Fluorescence Polarization’ the following tests may be performed to prove the specifications: • Precision/Repeatability ® These test instructions are valid for the infinite F200: •...
  • Page 139 8. Quality Control Plate Layout: <> Filling volume: 200 µl/well Material/Reagents: 1 nm Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377) 0.01 M NaOH (=Blank) (NaOH pellets, Merck Article No. 6495 or Sigma S8045) 1 Greiner 96-well plate, black. flat bottom 200 µl Pipette + tips Calculation of Precision: The precision is calculated from the wells filled with fluorescein.

  • Page 140: Glow Luminescence

    8. Quality Control 8.3.5 Glow Luminescence ® For the with the option ‘Luminescence the following tests may be infinite performed to prove the specifications: • Sensitivity Sensitivity Perform the following measurement to determine the Sensitivity: Measurement Parameters: Parameter Setting Reading Mode Luminescence Integration Time 1000 ms...

  • Page 141: Absorbance Accuracy

    8. Quality Control Calculation of the Sensitivity (Detection Limit): ⋅ − Stdev DetectionL imit fmol well 0002 − − mean mean 2*10 Concentration of ATP standard [M] Stdev Standard deviation of Blank mean Average of wells filled with ATP standard mean Average of Blank wells 0.0002...

  • Page 142: Absorbance Wavelength Accuracy

    8. Quality Control 8.3.7 Absorbance Wavelength Accuracy The wavelength accuracy defines the deviation of the set measurement wavelengths from the nominal wavelength. This test is only valid for the ® infinite M200 Measurement Parameters: Parameter Setting Reading Mode Absorbance Scan Measurement Wavelength from/to 300 –...

  • Page 143: Absorbance Baseline Flatness

    8. Quality Control ® 8.3.8 Absorbance Baseline Flatness (infinite M200) Perform the following measurement to determine the baseline-flatness: Measurement Parameters: Parameter Setting Reading Mode Absorbance Measurement Wavelength from/to 300 - 700 nm Number of flashes Settle Time Plate Type GRE96ft Part of the Plate Plate Layout: No plate is necessary for measurement –...

  • Page 144: Absorbance Baseline Flatness

    8. Quality Control ® 8.3.9 Absorbance Baseline Flatness (infinite F200) Perform the following measurements to determine the baseline-flatness with the available filter: Measurement Parameters: Parameter Setting Reading Mode Absorbance Measurement Wavelength 340 nm 405 nm 492 nm 590 nm 620 nm 700 nm Number of flashes Settle Time...

  • Page 145: M200 Only)

    8. Quality Control ® 8.3.10 Absorbance Cuvette (infinite M200 only) ® For the with the option ‘Cuvette’ the following test may be infinite M200 performed to prove the accuracy specification: Accuracy Perform the following measurement to determine the absorbance accuracy: Measurement Parameters: Parameter Setting...

  • Page 147: Cleaning And Maintenance

    9. Cleaning and Maintenance 9. Cleaning and Maintenance 9.1 Introduction Caution Ensure that the microplate is removed from the instrument before it is STOP prepared for shipment. If a microplate is left in the instrument, fluorescent solutions may spill onto the optical parts and damage the instrument.

  • Page 148: Liquid Spills

    9. Cleaning and Maintenance 9.2 Liquid Spills Switch OFF the instrument. Wipe up the spill immediately with absorbent material. Dispose of contaminated material appropriately. Clean the instrument surfaces with a mild detergent. For biohazardous spills clean with B30 (Orochemie, Max-Planck-Str. 27, D-70806 Kornwestheim).

  • Page 149: Instrument Disinfection

    9. Cleaning and Maintenance 9.3 Instrument Disinfection All parts of the instrument that come into contact with the patient samples, positive control samples or hazardous material must be treated as potentially infectious areas. WARNING THE DISINFECTION PROCEDURE SHOULD BE PERFORMED ACCORDING TO NATIONAL, REGIONAL, AND LOCAL REGULATIONS.

  • Page 150: Disinfection Procedure

    9. Cleaning and Maintenance 9.3.2 Disinfection Procedure WARNING THE DISINFECTION PROCEDURE SHOULD BE PERFORMED IN A WELL-VENTILATED ROOM BY AUTHORIZED TRAINED PERSONNEL WEARING DISPOSABLE POWDER-FREE GLOVES, PROTECTIVE GLASSES AND PROTECTIVE CLOTHING. If the laboratory has no specific disinfection procedure, the following procedure should be used to disinfect the outside surfaces of the instrument: 1.

  • Page 151: Disinfection Certificate

    9. Cleaning and Maintenance 9.4 Disinfection Certificate A disinfection certificate label MUST be completed and attached to the top of the package (visible from the outside of the shipping container!) in which the instrument is returned, before shipping it to the service center for service or repair.

  • Page 152: Disposal

    9. Cleaning and Maintenance 9.5 Disposal Follow laboratory procedures for bio-hazardous waste disposal, according to national and local regulations. This gives instructions on how to lawfully dispose of waste material accumulating in connection with the instrument. Caution STOP Observe all federal, state and local environmental regulations. ATTENTION DIRECTIVE 2002/96/EC ON WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT (WEEE)

  • Page 153: Disposal Of Packing Material

    9. Cleaning and Maintenance 9.5.1 Disposal of Packing Material According to Directive 94/62/EC on packaging and packaging waste, the manufacturer is responsible for the disposal of packing material. Returning Packing Material If you do not intend to keep the packing material for future use, e.g. for transport and storage purposes, return the packaging of the product, spare parts and options via the field service engineer to the manufacturer.

  • Page 154: Disposal Of The Instrument

    9. Cleaning and Maintenance 9.5.3 Disposal of the Instrument Please contact your local Tecan service representative before disposing of the instrument. Caution STOP Always disinfect the instrument before disposal. Pollution degree 2 (IEC/EN 61010-1) Method of disposal Contaminated waste WARNING...

  • Page 155: Troubleshooting

    Error Text Description Command is not valid Unspecific error in the Instrument - Computer communication protocol. Please report this error to your local Tecan customer support office. Parameter out of range Unspecific error in the Instrument - Computer communication protocol. Please report this error to your local Tecan customer support office.
  • Page 156 Tecan customer support office. "MTP is in Out-Position", Unspecific error in the Instrument - Computer communication protocol. Please report this error to your local Tecan customer support office. [val] ... not set at (Ratiolabel Unspecific error in the Instrument - Computer communication [n]) protocol.
  • Page 157 Wavelength:[lambda] Gain:[g], local Tecan customer support office. Counts: [cts] Steploss Error Actuator failure. Please report this error to your local Tecan customer support office. Sync Scan: Number of EX- Unspecific error in the Instrument - Computer communication Steps does not match EM- protocol.

  • Page 159: Index

    Index Index Numbers refer to pages. Packing Material ........155 Absorbance ..........19, 122 Emission Filter..........76 Absorbance Filter......... 90 Excitation............75 Absorbance Optics........90 Excitation Spot Size ........71 Baseline Flatness........145 Baseline Flatness Filter......146 Filter Switch Time.......... 100 Absorbance Detection ........
  • Page 160 Index Returning ........... 155 Installation............49 Instrument Features........117 PMT Properties..........98 Instrument Power On........96 Polarization............ 109 Power Requirements ........53 Instrument Start Up.......... 96 Integration Time..........115 Precision Intensities............109 Bottom Fluorescence......... 137 Fluorescence Polarization ......140 Kinetic Measurements ........97 Time resolved fluorescence.......

  • Page 161: Tecan Customer Support

    100022 Beijing China Tecan Deutschland GmbH Theodor-Storm-Strasse 17 T +49 1805 8322 633 or D-74564 Crailsheim T +49 1805 TECAN DE Germany F +49 7951 9417 92 helpdesk-de@tecan.com Tecan France S.A. 26 avenue Tony Garnier T +33 820 88 77 36...
  • Page 162 Durham, NC 27703 F +1 919 361 5201 Technical support for Toll Free in the US: Tecan instruments T +1 800 TECAN US or T +1 800 832 2687 helpdesk-us@tecan.com Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4 2008-07...

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