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Agilent Technologies 1200 Series User Manual

Standard and preparative autosamplers
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Agilent 1200 Series
Standard and Preparative
Autosamplers
User Manual
Agilent 1200 Series Autosampler User Manual
Agilent Technologies

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  • Page 1 Agilent 1200 Series Standard and Preparative Autosamplers User Manual Agilent 1200 Series Autosampler User Manual Agilent Technologies...
  • Page 2 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis- closure of Software is subject to Agilent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 1200 Series Autosampler User Manual...

  • Page 3: Table Of Contents

    Installing the Sample Tray Transporting the Autosampler 4 Using the Autosampler Solvent Information Sample Trays Choice of Vials and Caps 5 Optimizing Performance Optimization for Lowest Carry-over Fast Injection Cycle and Low Delay Volume Precise Injection Volume Agilent 1200 Series Autosampler User Manual...
  • Page 4 Multi-Draw Kit G1313-6871 900 µl Injection Upgrade Kit G1363A for G1329A External Tray G1313-60004 9 Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables External Contact Cable CAN/LAN Cables Auxiliary Cable RS-232 Cables Agilent 1200 Series Autosampler User Manual...
  • Page 5 Contents 10 Appendix General Safety Information Lithium Batteries Information Radio Interference Sound Emission Agilent Technologies on Internet Agilent 1200 Series Autosampler User Manual...
  • Page 6 Contents Agilent 1200 Series Autosampler User Manual...

  • Page 7: Introduction To The Autosampler

    Agilent 1200 Series Autosampler User Manual Introduction to the Autosampler Introduction to the Autosampler Sampling Sequence Injection Sequence Sampling Unit Needle-Drive Analytical head / preparative head Injection-Valve Transport Assembly Early Maintenance Feedback (EMF) Electrical Connections Agilent 1200 Series Interfaces Agilent Technologies...

  • Page 8: Introduction To The Autosampler

    The Agilent 1100 Series autosamplers and Agilent 1200 Series autosamplers are designed for use with other modules of the Agilent 1200 Series LC system, with the HP 1050 Series, or with other LC systems if adequate remote control inputs and outputs are available.
  • Page 9 Different valves are available for the standard and preparative autosamplers. Control of the vial temperature in the thermostatted autosampler is achieved using an additional Agilent 1200 Series module; the ALS thermostat. Details of this module are given in the Agilent 1200 Series thermostatted autosampler Supplemental Manual.

  • Page 10: Sampling Sequence

    9 The gripper arm checks if the safety flap is in position. 10 The gripper arm replaces the vial, and returns to the home position. Simultaneously, the needle lowers into the seat. 11 The injection valve switches to the mainpass position. Agilent 1200 Series Autosampler User Manual...

  • Page 11: Injection Sequence

    When the sample sequence begins, the valve unit switches to the bypass position (Figure 3 on page 11). Solvent from the pump enters the valve unit at port 1, and flows directly to the column through port 6. Figure 3 Bypass Position Agilent 1200 Series Autosampler User Manual...
  • Page 12 The needle is lowered into the needle seat, and the injection valve switches back to the mainpass position, flushing the sample onto the column (Figure 5 on page 12). Figure 5 Mainpass Position (Sample Injection) Agilent 1200 Series Autosampler User Manual...

  • Page 13: Sampling Unit

    The replacement sampling unit excludes the injection valve and metering head assemblies. N O T E The sampling units for the standard and the preparative autosamplers are different. Figure 6 Autosampler Sampling Unit Agilent 1200 Series Autosampler User Manual...

  • Page 14: Needle-Drive

    15000 15000 Volume resolution 7 nl/motor step 60 nl/motor step 60 nl/motor step Maximum stroke 100 µl 900 µl 900 µl Pressure limit 600 bar 200 bar 400 bar Plunger material Sapphire Sapphire Sapphire Agilent 1200 Series Autosampler User Manual...

  • Page 15: Injection-Valve

    4 V, 1.2 A stepper motor 4 V, 1.2 A stepper motor Seal material Vespel™ (Tefzel™ PEEK PEEK available) Stator material Ceramic/PEEK PEEK None Number of ports Switching time < 150 ms < 150 ms < 150 ms Agilent 1200 Series Autosampler User Manual...

  • Page 16: Transport Assembly

    The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly. Agilent 1200 Series Autosampler User Manual...
  • Page 17 These positions are used by the processor to calculate the actual motor position. An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly. Agilent 1200 Series Autosampler User Manual...

  • Page 18: Early Maintenance Feedback (Emf)

    The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled. For details on EMF counters and how to use them, see “Early Maintenance Feedback (EMF)” on page 82. Agilent 1200 Series Autosampler User Manual...

  • Page 19: Electrical Connections

    The security lever at the power input socket prevents the module cover from being taken off when line power is still connected. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.
  • Page 20 Introduction to the Autosampler Electrical Connections Figure 8 Autosampler (plus Thermostat) Electrical Connections Agilent 1200 Series Autosampler User Manual...

  • Page 21: Agilent 1200 Series Interfaces

    Introduction to the Autosampler Agilent 1200 Series Interfaces Agilent 1200 Series Interfaces The Agilent 1200 Series modules provide the following interfaces: Table 3 Agilent 1200 Series Interfaces Interface Type Pumps Autosampler DA Detector VW Detector Thermostatted Vacuum MW Detector RI Detector...
  • Page 22 Introduction to the Autosampler Agilent 1200 Series Interfaces Agilent 1200 Series Autosampler User Manual...

  • Page 23: Site Requirements And Specifications

    Agilent 1200 Series Autosampler User Manual Site Requirements and Specifications Site Requirements Power Consideration Power Cords Bench Space Environment Physical Specifications Performance Specifications Agilent Technologies...

  • Page 24: Site Requirements

    WA R N I N G Shock hazard or damage of your instrumentation can result, if the devices are connected to a line voltage higher than specified. ➔ Connect your instrument to the specified line voltage. Agilent 1200 Series Autosampler User Manual...

  • Page 25: Power Cords

    Never operate your instrumentation from a power outlet that has no ground connection. ➔ Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.

  • Page 26: Bench Space

    Ensure the autosampler is installed in a level position. If a complete Agilent 1200 Series system is to be installed on the bench, make sure that the bench is designed to carry the weight of all the modules. For a...

  • Page 27: Physical Specifications

    WA R N I N G Using the autosampler at high environmental temperatures may cause the rear panel to become hot. ➔ Do not use the autosampler at environmental temperatures higher than 50 °C (122 °F) Agilent 1200 Series Autosampler User Manual...

  • Page 28: Performance Specifications

    Site Requirements and Specifications Performance Specifications Performance Specifications Table 5 Performance Specifications Agilent 1200 Series Autosampler (G1329A). Valid when standard 100 µl metering head installed. Type Specification Pressure Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5900 psi)
  • Page 29 Site Requirements and Specifications Performance Specifications Table 6 Performance Specifications Agilent 1200 Series standard autosampler (G1329A). Valid when standard 900 µl metering head installed. Type Specification Pressure Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi)
  • Page 30 Site Requirements and Specifications Performance Specifications Table 7 Performance Specifications Agilent 1200 Series standard autosampler SL (G1329B). Type Specification Pressure Operating range 0 – 60 MPa (0 – 600 bar, 0 – 8850 psi) GLP features Early maintenance feedback (EMF), electronic records of...
  • Page 31 Site Requirements and Specifications Performance Specifications Table 8 Performance Specifications Agilent 1200 Series Preparative Autosampler (G2260A) Type Specification Pressure Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5800psi) GLP features Early maintenance feedback (EMF), electronic records of...
  • Page 32 Site Requirements and Specifications Performance Specifications Agilent 1200 Series Autosampler User Manual...

  • Page 33: Installing The Autosampler

    Agilent 1200 Series Autosampler User Manual Installing the Autosampler Unpacking the Autosampler Damaged Packaging Delivery Checklist Optimizing the Stack Configuration Installing the Autosampler Installing the Thermostatted Autosampler Flow Connections Installing the Sample Tray Transporting the Autosampler Agilent Technologies...

  • Page 34: Unpacking The Autosampler

    Table 9 on page 35 and Table 10 on page 36 are listed the content of each accessory kit. Please report missing or damaged parts to your local Agilent Technologies sales and service office. Agilent 1200 Series Autosampler User Manual...
  • Page 35 G1329-40301 Air channel adapter G1329-43200 Cover insulation no PN Capillary 0.17 mm, 900 mm G1329-87300 Capillary heat exchanger 01090-87306 Note for Agilent 1200 Series Autosampler door upgrade no PN Reorder gives pack of 15 Agilent 1200 Series Autosampler User Manual...
  • Page 36 Air channel adapter G1329-43200 Tray for 15 x 6 ml vials (x2) G1313-44513 Union, loop extension 5022-2133 Seat extension capillary (500 µl) G1313-87307 Seat extension capillary (1500 µl) G1313-87308 Sampler - Column capillary G2260-87300 Agilent 1200 Series Autosampler User Manual...

  • Page 37: Optimizing The Stack Configuration

    39 and Figure 12 page 40 show the configuration recommended for a thermostatted autosampler. These configurations optimize the system flow path, ensuring minimum delay volume. Figure 9 Recommended Stack Configuration for an Autosampler (Front View) Agilent 1200 Series Autosampler User Manual...
  • Page 38 Installing the Autosampler Optimizing the Stack Configuration Figure 10 Recommended Stack Configuration for an Autosampler (Rear View) Agilent 1200 Series Autosampler User Manual...
  • Page 39 Installing the Autosampler Optimizing the Stack Configuration Figure 11 Recommended Stack Configuration for a thermostatted ALS (Front View) Agilent 1200 Series Autosampler User Manual...
  • Page 40 Installing the Autosampler Optimizing the Stack Configuration Figure 12 Recommended Stack Configuration for a thermostatted ALS (Rear View) Agilent 1200 Series Autosampler User Manual...

  • Page 41: Installing The Autosampler

    Do not bend the safety flap away from its position, or attempt to remove the safety cover (see Figure 13 on page 42). ➔ Do not attempt to insert or remove a vial from the gripper when the gripper is positioned below the needle. Agilent 1200 Series Autosampler User Manual...
  • Page 42 5 Ensure the power switch at the front of the Autosampler is OFF. 6 Connect the power cable to the power connector at the rear of the sampler. 7 Connect the CAN cable to the other Agilent 1200 Series modules. 8 If an Agilent Chemstation is the controller, connect either •...
  • Page 43 Installing the Autosampler • The LAN connector to the LAN interface 9 Connect the APG remote cable (optional) for non Agilent 1200 Series instruments. 10 Turn ON power by pushing the button at the lower left hand side of the sampler.

  • Page 44: Installing The Thermostatted Autosampler

    ➔ Make sure the power cords are unplugged before disconnecting or reconnecting the autosampler to ALS thermostat cable. Agilent 1200 Series Autosampler User Manual...
  • Page 45 3 Remove the adhesive tape which covers the front door. 4 Remove the front door and remove the transport protection foam. 5 Place the Autosampler on top of the Thermostat. Make sure that the Autosampler is correctly engaged in the Thermostat locks. Agilent 1200 Series Autosampler User Manual...
  • Page 46 Figure 17 on page 47. 10 Connect the power cables to the power connectors. 11 Connect the CAN cable to the other Agilent 1200 Series modules. 12 If an Agilent ChemStation is the controller, connect either • The GPIB cable to the detector •...
  • Page 47 Installing the Autosampler Installing the Thermostatted Autosampler Figure 17 Cable Connections Agilent 1200 Series Autosampler User Manual...

  • Page 48: Flow Connections

    4 Ensure that the waste tube is positioned inside the leak channel. Do not extend the waste capillary of the autosampler. The siphoning effect might empty the N O T E complete seat capillary introducing air into the system. Agilent 1200 Series Autosampler User Manual...
  • Page 49 Installing the Autosampler Flow Connections Figure 18 Hydraulic Connections Agilent 1200 Series Autosampler User Manual...

  • Page 50: Installing The Sample Tray

    4 Press the front of the sample tray down to secure the tray in the autosampler. If the thermostatted autosampler tray pops out of position the air channel adapter is not N O T E inserted correctly. Figure 19 Installing the Sample Tray Agilent 1200 Series Autosampler User Manual...

  • Page 51: Transporting The Autosampler

    Also, ensure the vial tray is secured in place with suitable packaging, otherwise the tray may become loose and damage internal components. Agilent 1200 Series Autosampler User Manual...
  • Page 52 Installing the Autosampler Transporting the Autosampler Agilent 1200 Series Autosampler User Manual...

  • Page 53: Using The Autosampler

    Agilent 1200 Series Autosampler User Manual Using the Autosampler Solvent Information Sample Trays Choice of Vials and Caps Agilent Technologies...

  • Page 54: Solvent Information

    • Halogenated solvents or mixtures which form radicals and/or acids, for example: → 2CHCl 2COCl + 2HCl This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol. Agilent 1200 Series Autosampler User Manual...
  • Page 55 For example, a 1% solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). • Mixtures of carbon tetrachloride with 2-propanol or THF. Agilent 1200 Series Autosampler User Manual...

  • Page 56: Sample Trays

    Halftray for 15 x 6 ml vials (for G2260A only G1313-44513 This tray is not recommended when using a thermostat Half-Tray Combinations Half-trays can be installed in any combination enabling both 2 ml-and 6 ml-vials to be used simultaneously. Agilent 1200 Series Autosampler User Manual...
  • Page 57 101 as follows: Left-hand 40-position tray: 1 - 40 Left-hand 15-position tray: 1–15 Right-hand 40-position tray: 101–140 Right-hand 15-position tray: 101–115 Figure 20 Numbering of Tray Positions Agilent 1200 Series Autosampler User Manual...

  • Page 58: Choice Of Vials And Caps

    Choice of Vials and Caps List of Compatible Vials and Caps For reliable operation vials used with the Agilent 1200 Series autosampler must not have tapered shoulders or caps that are wider than the body of the vial. The vials in...
  • Page 59 Clear PTFE/red rubber 5181-1217 Table 16 Snap Caps Description Septa 100/Pack Clear polypropylene Clear PTFE/red rubber 5182-0550 Blue polypropylene Clear PTFE/red rubber 5182-3458 Green polypropylene Clear PTFE/red rubber 5182-3457 Red polypropylene Clear PTFE/red rubber 5182-3459 Agilent 1200 Series Autosampler User Manual...
  • Page 60 100/Pack Blue polypropylene Clear PTFE/red rubber 5182-0717 Green polypropylene Clear PTFE/red rubber 5182-0718 Red polypropylene Clear PTFE/red rubber 5182-0719 Blue polypropylene Clear PTFE/silicone 5182-0720 Green polypropylene Clear PTFE/silicone 5182-0721 Red polypropylene Clear PTFE/silicone 5182-0722 Agilent 1200 Series Autosampler User Manual...

  • Page 61: Optimizing Performance

    Agilent 1200 Series Autosampler User Manual Optimizing Performance Optimization for Lowest Carry-over Using the Automated Needle Wash Using an Injector Program General Recommendation to Lowest Carry-over Fast Injection Cycle and Low Delay Volume Overlapped Injection Mode General Recommendations for Fast Injection Cycle Times...

  • Page 62: Optimization For Lowest Carry-Over

    When using small injection volumes or when injecting samples of low concentration immediately after samples of high concentration, carry-over may become noticeable. Using the automated needle wash enables the carry-over to be minimized and prevents also contamination of the needle seat. Agilent 1200 Series Autosampler User Manual...

  • Page 63: Using The Automated Needle Wash

    2 NEEDLE WASH vial 7 3 INJECT Line 1 draws 5 µl from the current sample vial. Line 2 moves the needle to vial 7. Line 3 injects the sample (valve switches to main pass). Agilent 1200 Series Autosampler User Manual...

  • Page 64: Using An Injector Program

    Inject Wait (equilibration time - see text above) Valve bypass Wait 0.2 min Valve mainpass Valve bypass Valve mainpass Overlapped injection together with additional injection valve switching is not possible. N O T E Agilent 1200 Series Autosampler User Manual...

  • Page 65: General Recommendation To Lowest Carry-Over

    If this is not known use 2 or 3 solvents of different polarity. Use several milliliters to clean the seat. • Clean the needle seat with a tissue and remove all liquid from it. • RESET the injector. Agilent 1200 Series Autosampler User Manual...

  • Page 66: Fast Injection Cycle And Low Delay Volume

    100 µl instead of 1 µl, increase the injection time by approximately 8 sec. In this case and if the viscosity of the sample allows it, the draw and eject speed of the injection system has to be increased. Agilent 1200 Series Autosampler User Manual...

  • Page 67: General Recommendations For Fast Injection Cycle Times

    • Increase of draw and eject speed for large injection volumes • Add at last run a blank, if overlapped injection is used To reduce the injection time, the detector balance has to be set to OFF. Agilent 1200 Series Autosampler User Manual...

  • Page 68: Precise Injection Volume

    When using injection volumes < 2 µl, these gas bubbles may affect the injection-volume precision. For best injection-volume precision with injection volumes < 2 µl, use of the Agilent 1200 Series degasser is recommended to ensure the mobile phase is adequately degassed. Also, using the automated needle wash (see “Optimization for Lowest...
  • Page 69 An “EJECT” statement in an injector program also uses the eject speed setting which is configured for the autosampler. A faster eject speed shortens the time required to run the injector program. When using viscous samples, a high eject speed should be avoided. Agilent 1200 Series Autosampler User Manual...

  • Page 70: Choice Of Rotor Seal

    1 and 14. This seal is also used for the G1329B module. Strong oxidizing acids such as concentrated nitric and sulfuric acids are not compatible N O T E with PEEK. Agilent 1200 Series Autosampler User Manual...

  • Page 71: Troubleshooting And Diagnostics

    Agilent 1200 Series Autosampler User Manual Troubleshooting and Diagnostics Agilent Lab Monitor & Diagnostic Software Overview of the Sampler’s Indicators and Test Functions Agilent Technologies...

  • Page 72: Agilent Lab Monitor & Diagnostic Software

    Agilent LC or all the Agilent GCs and LCs configured on the lab intranet. Agilent Lab Advisor provides diagnostic capabilities for all Agilent 1200 Series HPLC modules. This includes tests and calibrations procedures as well as the different injector steps to perform all the maintenance routines.

  • Page 73: Overview Of The Sampler's Indicators And Test Functions

    Step Commands The step functions provide the possibility to execute each step of the sampling sequence individually. The step functions are used primarily for troubleshooting, and for verification of correct autosampler operation after repair. Agilent 1200 Series Autosampler User Manual...
  • Page 74 Troubleshooting and Diagnostics Overview of the Sampler’s Indicators and Test Functions Agilent 1200 Series Autosampler User Manual...

  • Page 75: Maintenance

    Agilent 1200 Series Autosampler User Manual Maintenance Introduction into Maintenance and Repair Simple Repairs Exchanging Internal Parts Safety Flap, Flex Board Transport Assembly Parts Updating the Firmware Warnings and Cautions Using the ESD Strap Cleaning the module Overview of Main Repair Procedures...

  • Page 76: Introduction Into Maintenance And Repair

    Agilent-trained service personnel. There are no other field-replaceable parts in the transport assembly. If any other component is defective (flex board, spindles, plastic parts) the complete unit must be exchanged. Agilent 1200 Series Autosampler User Manual...

  • Page 77: Updating The Firmware

    Introduction into Maintenance and Repair Updating the Firmware The Agilent 1200 Series LC modules are fitted with FLASH EPROMS. These EPROMS enable you to update the instrument firmware from the ChemStation, PCMCIA card, or through the RS232 interface. The firmware update procedure is described in the on-line user information.

  • Page 78: Warnings And Cautions

    Electronic boards are sensitive to electronic discharge (ESD). In order to prevent damage, always use an ESD kit when handling electronic boards and components. Use the ESD kit according to recommendations given by the supplier. Agilent 1200 Series Autosampler User Manual...

  • Page 79: Cleaning The Module

    WA R N I N G Liquid in the module electronics can cause shock hazard and damage the module. ➔ Do not use an exessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any fittings. Agilent 1200 Series Autosampler User Manual...

  • Page 80: Overview Of Main Repair Procedures

    Maintenance Overview of Main Repair Procedures Overview of Main Repair Procedures Figure 21 Main Assemblies Agilent 1200 Series Autosampler User Manual...
  • Page 81 Power supply ASM board Transport assembly Metering seal, “Metering Seal and Plunger” on page 98 Needle, “Needle Assembly” on page 86 Rotor seal, “Rotor Seal” on page 94 Needle seat, “Needle-Seat Assembly” on page 89 Agilent 1200 Series Autosampler User Manual...

  • Page 82: Early Maintenance Feedback (Emf)

    This counter display the total number of switches of the injection valve since the last reset of the counter. Needle Movements Counter This counter displays the total number of movements of the needle into the seat since the last reset of the counter. Agilent 1200 Series Autosampler User Manual...

  • Page 83: Using The Emf Counters

    EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. Agilent 1200 Series Autosampler User Manual...

  • Page 84: Maintenance Functions

    Certain maintenance procedures require the needle arm, metering device, and gripper assembly to be moved to specific positions to enable easy access to components. The maintenance functions move these assemblies into the appropriate maintenance position. Agilent 1200 Series Autosampler User Manual...

  • Page 85: Simple Repairs

    Exchanging the When autosampler reproducibility 30 minutes “Metering Seal and Plunger” metering seal indicates seal wear page 98 Exchanging the gripper When the gripper arm is defective 10 minutes “Gripper Arm” on page 102 Agilent 1200 Series Autosampler User Manual...

  • Page 86: Needle Assembly

    To avoid personal injury, keep fingers away from the needle area during autosampler operation. ➔ Do not bend the safety flap away from its position, or attempt to insert or remove a vial from the gripper when the gripper is positioned below the needle. Agilent 1200 Series Autosampler User Manual...
  • Page 87 Remove the sample-loop fitting from the needle fitting. with the hole in the safety cover. Loosen the fixing screw, and lift out the needle. Insert the new needle. Align the needle in the seat, then tighten the screw firmly. Agilent 1200 Series Autosampler User Manual...
  • Page 88 Use Needle Up to lift the needle to a position approximately 2mm above the seat. Ensure the needle is aligned with the seat. Next Steps: On completion of this procedure: Install the front cover. Select “End” in the maintenance function “Change Needle”. Agilent 1200 Series Autosampler User Manual...

  • Page 89: Needle-Seat Assembly

    • Use the “Needle Up” command in the “Change Needle” function to lift the needle an addition 1 Disconnect the seat-capillary fitting from the injection Use a small flat-head screwdriver to ease out the needle valve (port 5). seat. Agilent 1200 Series Autosampler User Manual...
  • Page 90 Ensure the needle is aligned with the seat. If required, above the seat bend the needle slightly until the needle is aligned correctly. Next Steps: On completion of this procedure: Install the front cover. Select “End” in the maintenance function “Change Needle”. Agilent 1200 Series Autosampler User Manual...

  • Page 91: Stator Face

    Carefully handle the valve to prevent damage to the stator face Remove all capillary fittings from the injection-valve ports Loosen each fixing bolt two turns at a time. Remove the bolts from the head. Agilent 1200 Series Autosampler User Manual...
  • Page 92 Install stator head and stator face. Tighten the bolts Reconnect the pump capillaries to the valve ports (see alternately two turns at a time until the stator head is Figure 18 on page 49). secure. Agilent 1200 Series Autosampler User Manual...
  • Page 93 Maintenance Simple Repairs Slide the waste tube into the waste holder in the leak tray. On completion of this procedure: Install the front cover. Agilent 1200 Series Autosampler User Manual...

  • Page 94: Rotor Seal

    The stator face is held in place by the stator head. When you remove the stator head, the stator face can fall out of the valve. ➔ Carefully handle the valve to prevent damage to the stator face There is no stator face for G1329B. N O T E Agilent 1200 Series Autosampler User Manual...
  • Page 95 Remove all capillary fittings from the injection-valve ports. Loosen each fixing bolt two turns at a time. Remove the bolts from the head. Remove the stator head, stator face and stator ring. Remove the rotor seal and isolation seal. Agilent 1200 Series Autosampler User Manual...
  • Page 96 Place the stator face in place on the stator head. Install stator head and stator face. Tighten the bolts alternately two turns at a time until the stator head is secure. Agilent 1200 Series Autosampler User Manual...
  • Page 97 Reconnect the pump capillaries to the valve ports (see Slide the waste tube into the waste holder in the leak tray. Figure 18 on page 49). On completion of this procedure: Install the front cover. Agilent 1200 Series Autosampler User Manual...

  • Page 98: Metering Seal And Plunger

    • Remove the front cover. Remove the two capillaries from the metering-head Remove the two fixing bolts, and pull the head assembly assembly. away from the sampler. Notice that the closed side of the metering head faces upwards. Agilent 1200 Series Autosampler User Manual...
  • Page 99 Disassemble the metering head assembly. head assembly. Use a small screwdriver to carefully remove the seal. Install the new seal. Press the seal firmly into position. Clean the chamber with lint-free cloth. Ensure all particular matter is removed. Agilent 1200 Series Autosampler User Manual...
  • Page 100 Install the fixing bolts. Tighten the bolts securely. Install the metering head assembly in the autosampler. Ensure the large hole in the metering head is facing downwards. Agilent 1200 Series Autosampler User Manual...
  • Page 101 Maintenance Simple Repairs Reinstall the capillaries. Next Steps: On completion of this procedure: Install the front cover. Select “End” in the maintenance function “Change piston” . Agilent 1200 Series Autosampler User Manual...

  • Page 102: Gripper Arm

    • Remove the front cover. Identify the slit below the gripper motor and the gripper Rotate the arm approximately 2.5cm (1 inch) to the left arm release button. and insert the straightened paper clip into the slit. Agilent 1200 Series Autosampler User Manual...
  • Page 103 Replace the gripper arm by holding the paper clip in place, pushing the gripper arm into the holder and rotating the gripper arm to the left. Next Steps: On completion of this procedure: Install the front cover. Turn the power to the autosampler ON. Agilent 1200 Series Autosampler User Manual...

  • Page 104: Interface Board

    3 Loosen the screws. Slide out the interface board from the autosampler. 4 Install the interface board. Secure the screws. 5 Reconnect the cables to the board connectors Figure 22 Exchanging the Interface Board Agilent 1200 Series Autosampler User Manual...

  • Page 105: Parts And Materials For Maintenance

    Agilent 1200 Series Autosampler User Manual Parts and Materials for Maintenance Main Assemblies Analytical-Head Assembly Vial Trays Standard Autosampler Accessory Kit G1329-68725 Preparative Autosampler Accessory Kit G2260-68705 Maintenance Kit G1313-68730 for G1329A Maintenance Kit G1313-68719 for G1329B Multi-Draw Kit G1313-6871 900 µl Injection Upgrade Kit G1363A for G1329A...

  • Page 106: Main Assemblies

    Parts and Materials for Maintenance Main Assemblies Main Assemblies Figure 23 Autosampler Main Assemblies Agilent 1200 Series Autosampler User Manual...
  • Page 107 G2260-87300 Power supply assembly (part not shown) 0950-2528 Screw M4, 8 mm lg - power supply (part not shown) 0515-0910 BCD board (not shown) G1351-68701 Cable, autosampler to ALS thermostat (part not shown) G1330-81600 Agilent 1200 Series Autosampler User Manual...

  • Page 108: Analytical-Head Assembly

    Screw M4, 40 mm lg, for mounting of assembly 0515-0850 Adapter 01078-23202 Support seal assembly 5001-3739 Metering seal (pack of 2) 5063-6589 Head body 01078-27710 Screw M5, 60 mm lg, for mounting of assembly (not shown 0515-2118 here) Agilent 1200 Series Autosampler User Manual...
  • Page 109 Parts and Materials for Maintenance Analytical-Head Assembly Figure 24 Analytical-Head Assembly Agilent 1200 Series Autosampler User Manual...
  • Page 110 Screw M5, 60 mm lg, for mounting of assembly (not shown 0515-2118 here) This head is limited to 400 Bars. It can only be assembled on a sampling unit with the description “supports 900 µl at 400 Bar. Agilent 1200 Series Autosampler User Manual...

  • Page 111: Vial Trays

    Tray base for G1329A / G1329B / G2260A (includes items 4, 5). G1329-60000 Spring stud 0570-1574 Halftray for 40 × 2-ml vials (not shown) G1313-44512 Halftray for 15 × 6-ml vials (not shown) G1313-44513 Figure 25 Thermostatted Autosampler Vial Trays and Tray Base Agilent 1200 Series Autosampler User Manual...

  • Page 112: Standard Autosampler Accessory Kit G1329-68725

    Front door cooled autosampler no PN Air channel adapter G1329-43200 Cover insulation no PN Capillary 0.17 mm, 900 mm G1329-87300 Capillary heat exchanger 01090-87306 Note for Agilent 1200 Series Autosampler door upgrade no PN Agilent 1200 Series Autosampler User Manual...

  • Page 113: Preparative Autosampler Accessory Kit G2260-68705

    Tray for 15 x 6 ml vials (x2) G1313-44513 Union, loop extension 5022-2133 Seat extension capillary (500 µl) G1313-87307 Seat extension capillary (1500 µl) G1313-87308 Sampler - Column capillary G2260-87300 Pump - Sampler capillary G2260-87301 Agilent 1200 Series Autosampler User Manual...

  • Page 114: Maintenance Kit G1313-68730 For G1329A

    Maintenance Kit G1313-68730 for G1329A Maintenance Kit G1313-68730 for G1329A Table 26 Maintenance Kit for G1329A Item Description Part Number Rotor seal (Vespel) 0100-1853 Needle assembly (100 µl) G1313-87201 Needle-seat assembly 0.17 mm, 2.3 µl G1313-87101 Agilent 1200 Series Autosampler User Manual...

  • Page 115: Maintenance Kit G1313-68719 For G1329B

    Maintenance Kit for G1329A Item Description Part Number Rotor seal (PEEK) 0101-1416 Needle assembly (100 µl) G1313-87201 Needle-seat assembly 0.17 mm G1313-87101 Metering seal (pack of 2) 5063-6589 Finger caps (pack of 15) 5063-6506 Agilent 1200 Series Autosampler User Manual...

  • Page 116: Multi-Draw Kit G1313-6871

    Table 28 Multi-Draw Kit for G1329A and G1329B Item Description Part Number Seat capillary, 500 µl, 0.5 mm id G1313-87307 Seat capillary, 1500 µl, 0.9 mm id G1313-87308 Seat capillary, 5000 µl 0101-0301 Union 5022-6515 Agilent 1200 Series Autosampler User Manual...

  • Page 117: 900 Μl Injection Upgrade Kit G1363A For G1329A

    900 µl Injection Upgrade Kit G1363A for G1329A Table 29 900 µl Injection Upgrade Kit for G1329A only Item Description Part Number Analytical Head, 900 µl G1313-60007 Loop Extension, 900 µl G1313-87303 Union, loop extension 5022-2133 Needle, 900 µl G1313-87202 Agilent 1200 Series Autosampler User Manual...

  • Page 118: External Tray G1313-60004

    Parts and Materials for Maintenance External Tray G1313-60004 External Tray G1313-60004 Table 30 External Tray Item Description Part Number External tray G1313-60004 Disposal tube G1313-27302 Agilent 1200 Series Autosampler User Manual...

  • Page 119: Identifying Cables

    Agilent 1200 Series Autosampler User Manual Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables External Contact Cable CAN/LAN Cables Auxiliary Cable RS-232 Cables Agilent Technologies...

  • Page 120: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Type Description Part Number Analog cables 3390/2/3 integrators...
  • Page 121 Part Number CAN cables Agilent 1100/1200 module to module,0.5m lg 5181-1516 Agilent 1100/1200 module to module, 1m lg 5181-1519 External Agilent 1100/1200 Series interface board to general G1103-61611 contacts purpose GPIB cable Agilent 1100/1200 module to ChemStation, 1 m 10833A...

  • Page 122: Analog Cables

    Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent 1100/1200 Series modules. The other end depends on the instrument to which connection is being made. Agilent 1100/1200 to 3390/2/3 Integrators Connector 01040-60101...
  • Page 123 Pin Agilent Signal Name 1100/1200 Not connected Shield Analog - Center Analog + Agilent 1100/1200 to BNC Connector Connector8120-1840 Pin BNC Pin Agilent Signal Name 1100/1200 Shield Shield Analog - Center Center Analog + Agilent 1200 Series Autosampler User Manual...
  • Page 124 Identifying Cables Analog Cables Agilent 1100/1200 to General Purpose Connector01046-60105 Pin 3394/6 Pin Agilent Signal Name 1100/1200 Not connected Black Analog - Analog + Agilent 1200 Series Autosampler User Manual...

  • Page 125: Remote Cables

    Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent 1100/1200 Series modules. The other end depends on the instrument to be connected to.
  • Page 126 4 - Blue Shut down 5 - Pink Not connected 6 - Yellow Power on High 5,14 7 - Red Ready High 8 - Green Stop 9 - Black Start request 13, 15 Not connected Agilent 1200 Series Autosampler User Manual...
  • Page 127 13, 15 Not connected Agilent 1100/1200 to 3396 Series II / 3395A Integrators Use the cable and cut pin #5 on the integrator side. part number: 03394-60600 Otherwise the integrator prints START; not ready. Agilent 1200 Series Autosampler User Manual...
  • Page 128 Not connected 6 - Yellow 6 - Yellow Power on High 7 - Red 7 - Red Ready High 8 - Green 8 - Green Stop 9 - Black 9 - Black Start request Agilent 1200 Series Autosampler User Manual...
  • Page 129 3 - Gray Start 4 - Blue Shut down 5 - Pink Not connected 6 - Yellow Power on High 7 - Red Ready High 8 - Green Stop 9 - Black Start request Agilent 1200 Series Autosampler User Manual...

  • Page 130: Bcd Cables

    BCD Cables BCD Cables One end of these cables provides a 15-pin BCD connector to be connected to the Agilent 1200 Series modules. The other end depends on the instrument to be connected to Agilent 1200 to General Purpose Connector G1351-81600...
  • Page 131 Agilent 1200 to 3396 Integrators Connector03396-60560 Pin 3392/3 Pin Agilent Signal Name BCD Digit 1200 BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V Agilent 1200 Series Autosampler User Manual...

  • Page 132: External Contact Cable

    External Contact Cable One end of this cable provides a 15-pin plug to be connected to Agilent 1200 Series module’s interface board. The other end is for general purpose. Agilent 1200 Series Interface Board to general purposes Connector G1103-61611 Color...

  • Page 133: Can/Lan Cables

    Agilent 1200 module to control module G1323-81600 LAN Cables Description Part number Cross-over network cable (shielded, 3 m long), (for point to 5023-0203 point connection) Twisted pair network cable (shielded, 7 m long) (for hub 5023-0202 connections) Agilent 1200 Series Autosampler User Manual...

  • Page 134: Auxiliary Cable

    Agilent 1100 Series vacuum degasser. The other end is for general purpose. Agilent 1100 Series Degasser to general purposes Connector G1322-81600 Color Pin Agilent Signal Name 1100 White Ground Brown Pressure signal Green Yellow Grey DC + 5 V IN Pink Vent Agilent 1200 Series Autosampler User Manual...

  • Page 135: Rs-232 Cables

    This kit contains a 9-pin female to 9-pin female Null Modem 34398A (printer) cable and one adapter. Use the cable and adapter to connect Agilent Technologies instruments with 9-pin male RS-232 connectors to most PCs or printers. Agilent 1200 Series Autosampler User Manual...
  • Page 136 Identifying Cables RS-232 Cables Agilent 1200 Series Autosampler User Manual...

  • Page 137: Appendix

    Agilent 1200 Series Autosampler User Manual Appendix General Safety Information Lithium Batteries Information Radio Interference Sound Emission Agilent Technologies on Internet Agilent Technologies...

  • Page 138: General Safety Information

    The operator of this instrument is advised to use the equipment in a manner as specified in this manual. General This is a Safety Class I instrument (provided with terminal for protective earthing) and has been manufactured and tested according to international safety standards. Agilent 1200 Series Autosampler User Manual...
  • Page 139 Capacitors inside the instrument may still be charged, even though the instrument has been disconnected from its source of supply. Dangerous voltages, capable of causing serious personal injury, are present in this instrument. Use extreme caution when handling, testing and adjusting. Agilent 1200 Series Autosampler User Manual...
  • Page 140 When working with solvents please observe appropriate safety procedures (e.g. goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. Agilent 1200 Series Autosampler User Manual...
  • Page 141 C A U T I O N alerts you to situations that could cause loss of data, or damage of equipment. ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent 1200 Series Autosampler User Manual...

  • Page 142: Lithium Batteries Information

    Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten. ➔ Brukt batteri returneres appararleverandoren. Bij dit apparaat zijn batterijen geleverd. Wanneer deze leeg zijn, moet u ze niet weggooien N O T E maar inleveren als KCA. Agilent 1200 Series Autosampler User Manual...

  • Page 143: Radio Interference

    If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises. Agilent 1200 Series Autosampler User Manual...

  • Page 144: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 1200 Series Autosampler User Manual...

  • Page 145: Agilent Technologies On Internet

    For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select Products/Chemical Analysis It will provide also the latest firmware of the Agilent 1200 Series modules for download. Agilent 1200 Series Autosampler User Manual...
  • Page 146 2 µl electrical connections injection volumes descriptions of connecting APG remote injection sequence electrostatic discharge (ESD) connecting the ChemStation injection valve environment connecting the power installation connecting CAN error messages Agilent 1200 Series Autosampler User Manual...
  • Page 147 I parts and materials metering seal 900 µl 110, analytical-head assembly (optional safety information metering seal 900 microlitre) lithium batteries missing parts analytical-head assembly safety multi-draw option Agilent 1200 Series Autosampler User Manual...
  • Page 148 Z-axis stack configuration rear view standard autosampler accessory kit contents stator status indicator step functions stepper motor temperature theta-axis transport assembly transport mechanism transport unpacking the autosampler valve capillaries vial contents temperature Agilent 1200 Series Autosampler User Manual...
  • Page 149 In This Book This manual contains user information about the Agilent 1200 Series Standard and Preparative Autosamplers. The manual describes the following: • introduction to the autosampler, • site requirements and specifications • installing the autosampler, • using the autosampler, •...

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