Incubators - Hologic Panther System Operator's Manual

Table 17. Panther Mid Bay Descriptions (Continued)
Location Description
9. Sample Tip Eject
10. Sample Dispense Slot
11. Output Queue
12. Chiller Ramp Slots 1–6
13. Luminometer
14. Distributor
15. MTU Input Queue
16. AMP Incubator (Includes Fluorometer)*
17. High Temperature Incubator
18. Transition Incubator
*The Fluorometer is only included under certain system software configurations.

Incubators

The instrument contains three (3) incubators that are monitored and controlled by dual
internal thermistors. The incubators control the temperature of the MTUs during the assay
process.
The instrument contains the following incubators:
High Temperature (Anneal) Incubator—Binds target nucleic acid to capture
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oligonucleotides during Target Capture; Binds amplification primers to purified target
nucleic acid during TMA; Hybridizes AE-labeled probes to the amplicon during HPA
and destroys AE on unhybridized probes.
Transition Incubator—Binds target nucleic acid to magnetic microparticles; Cools
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the MTU before amplification; Cools the MTU after HPA and before entering the
luminometer.
Amplification Incubator (AMP)—Amplifies targets during TMA. Attached beneath the
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Amplification Incubator are the fluorometers used to detect fluorescent signals from
real-time assays.
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Panther System Operator's Manual
AW-17791-001 Rev. 002 (EN)
Overview
37