Loading Panther Assay Reagents - Hologic Panther System Operator's Manual

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Caution—Inspect Assay Reagents for foam, bubbles, and undissolved
particles before loading them on the Panther System. Do not use
reagents with foam, bubbles, or undissolved particles in the Panther
System.
4. Slide the bottles into the correct position in an appropriately sized Reagent Rack.
5. Verify that the barcode for each bottle is visible through the slot in the rack.

Loading Panther Assay Reagents

Caution—Do not use assay reagent kits on multiple Panther Systems.
Onboard stability tracking will be inaccurate and may lead to assay failure.
The system groups a set of assay reagents the first time that they are loaded. They
are then required to be run as a "matched kit" in all subsequent worklists. Bottles
belonging to a matched kit cannot be swapped with bottles in other sets of assay
reagents. Use the Assay Reagent Report to identify bottle IDs belonging to a
matched set (see Assay Reagent Report on page 166).
Caution—Spilling the contents of assay reagent bottles before loading may
lead to assay failure. The Panther System updates the remaining tests
available in an assay reagent kit following a spill before pipetting the reagent.
A loss of volume in the Amplification, Enzyme, Probe, and Selection Reagents
may impact the ability of the system to complete assay processing.
To load Panther Assay Reagents:
1. Open the Reagent Bay Door.
The Panther Assay Reagent Bay screen opens.
2. Lift the rack by the handle in the back, supporting the rack with the other
hand.
3. Before loading the rack into a Reagent Bay Lane, ensure that the onscreen
Reagent Bay Lane graphic is bordered in purple and the Reagent Bay Lane
LED is flashing green.
4. After the conditions in Step 3 have been met, gently slide the Reagent Rack
into the appropriate lane in the Reagent Bay until it reaches the back of the
bay.
The Reagent Barcode Reader will automatically scan the Reagent Rack
and assay reagent barcodes as the rack is loaded.
If the Reagent Rack was successfully loaded onto the system, the TCR
Carousel stops spinning and position 1 is rotated to the front of the
instrument. This process may be delayed if reagents are currently being
pipetted. Once the TCR Carousel stops the TCR Door unlocks.
®
Panther System
Operator's Manual
AW-17791-001 Rev. 002 (EN)
System Operation
110

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