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5. Load up to four (4) Reagent Racks as necessary, following steps 2–4. Close
the Reagent Bay Door after all Reagent Racks have been loaded.
6. Open the TCR Door.
7. Load the corresponding Target Capture Reagent (TCR) and Target
Enhancer Reagent (TER), if necessary, into the TCR Carousel. If multiple
Reagent Racks were loaded, multiple TCR and TER bottles must be loaded
as appropriate.
To load TCR and TER:
a. Mix the TCR and TER bottles thoroughly by gentle inversion prior to
loading on the carousel. Avoid creating foam.
b. Visually inspect the reagents for foam, bubbles, and undissolved
particles.
c. Ensure the appropriate size TCR Adapter is loaded into the needed
TCR Carousel position.
d. Slide the TCR bottle into the TCR cup that matches the Reagent Bay
Lane number of the corresponding assay reagent kit. Press down firmly
on the TCR bottle to ensure that the bottle is fully inserted in the cup.
e. If needed, slide the TER bottle into the TER cup that matches the
Reagent Bay Lane number of the corresponding assay reagent kit.
Press down firmly on the TER bottle to ensure that the bottle is fully
inserted in the cup.
f. Verify that the barcodes of the TCR and TER bottles are visible through
the slots in the cups. If necessary, rotate the TCR Carousel to ensure
the proper placement of the bottles.
8. Load up to four (4) bottles of TCR and TER to correspond to the racks
loaded in the Reagent Bay, following step 7.
9. Close the TCR Door once all TCR and TER bottles have been loaded.
The TCR Carousel starts to rotate and the system scans all TCR Carousel
positions. TCR or TER pipetting will resume if a run was previously in
progress. The Supplemental Reagent Information window opens for the
operator to enter the reconstitution dates and TCR additive for each new kit
that was loaded, as necessary.
Figure 56. Supplemental Reagent Information Window (Example)
®
Panther System
Operator's Manual
AW-17791-001 Rev. 002 (EN)
System Operation
111
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