Appendix A. Panther System Operation Checklist - Hologic Panther System Operator's Manual

Appendix A. Panther System Operation Checklist

Instrument & Laboratory Check
Check room humidity (20%–85%)
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Check room temperature (15°C–30°C)
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Perform external inspection
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Check for leaks
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Prepare Workspace
Clean work areas
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Change Gloves
Prepare Assay Reagents
Print Assay Reagents Report, if necessary
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Reconstitute reagents, if necessary
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If using previously reconstituted reagents, bring reagents to
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room temperature and gently invert all reagents
Warm Probe Reagent, if necessary, and invert
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Change Gloves
Complete Panther System Tasks
1. Load Tips
2. Load MTUs
Check for:
All 5 tiplets present
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All tube positions are free of debris
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Barcodes present, properly aligned, and undamaged
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Distributor foot is undamaged
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3. Load Universal Fluids
Replace depleted fluids
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Ensure all fittings are secure
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4. Empty Waste
Ensure all fittings are secure
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Change Gloves
5. Perform Maintenance
Complete all required maintenance tasks
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6. Prime
Ensure resources are available
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Panther System Operator's Manual
AW-17791-001 Rev. 002 (EN)
Appendix A. Panther System Operation Checklist
7. Load Assay Reagents
Gently invert reagents, remove caps, ensure no
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bubbles and/or precipitate is present
Ensure bottles are properly seated
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Verify all barcodes are visible
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Load onto the Panther System
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Ensure wTCR position matches assay reagent lane for
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corresponding reagents
8. Load Samples
Ensure sample default settings are accurate or test
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orders are available via LIS
Rack calibrators and/or controls for each assay reagent
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kit loaded, if necessary
Confirm correct sample collection and volume
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Place samples into racks
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Verify all barcodes are visible
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If required, ensure sample retainers are seated
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Load racks onto the Panther System
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Change Gloves
Pipetting & Assay Processing
Sample tube graphic will change from:
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Green—Sample loaded
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Yellow—Pipetting in-process
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Blue—Pipetting complete
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Feed & Monitor
Return, as needed, to load tips, MTUs, additional sample
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racks, and Assay Reagents
Change Gloves
Review/Manage Results
Send to LIS, if necessary
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Print Results Report, if necessary
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Unload Assay Reagents (optional)
Cap and appropriately store assay reagents.
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Log Off (optional)
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