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Dual Kinetic Assay (DKA)
Aptima assays may also utilize the Dual Kinetic Assay (DKA) technology to enable
multiplex detection from a single sample. In DKA, differences in the kinetic profiles of
labeled probes allow for the differentiation of signal; kinetic profiles are derived from
measurements of photon output during the detection read time. When the
chemiluminescent detection reaction for the signal has very rapid kinetics, it is referred to
as a "flasher" kinetic type. When the chemiluminescent detection reaction for the signal is
relatively slower, it is referred to as a "glower" kinetic type.
Real-Time Assays
Real-time assay technology utilizes the target capture and the Transcription-Mediated
Amplification processes. Once the enzyme reagent is added, the progress of the
amplification is continually monitored with fluorescence detection. Several target nucleic
acids can be monitored and specifically identified, based on the wavelength of the
fluorescent molecule. The amplification reagent contains fluorescently labeled
oligonucleotides (torches) that have the two ends hybridized together (hairpin loop). On
one end is the fluorescent label and the other end has a quencher molecule. When the
two ends are in close proximity, the quencher molecule prevents the fluorescent light from
being released. During amplification, as the amplicon is being produced, the torch
hybridizes to the amplicon. When the torch is hybridized, the quencher is no longer next
to the fluorescent molecule, thereby allowing fluorescent light to be emitted. As the
amplification continues, the amount of bound torch reaches the detection threshold of the
fluorescence detector.
Internal Controls for Aptima Assays
Aptima assays may use an Internal Control to control for nucleic acid capture,
amplification, and detection, as well as operator or instrument error. Internal control is
added to each reaction. Internal Control signal in each reaction is discriminated from the
target signal.
Panther System Overview
Introduction
The Panther System is an integrated nucleic acid testing system that fully automates all
steps necessary to perform Hologic assays from sample processing through amplification,
detection, and data reduction.
Instrument Overview
The Panther System consists of the instrumentation and computer together in a single
unit. The instrument is operated via the touch-screen monitor that is connected to the
system. Communication with the instrument is controlled through a number of processing
boards known as the Communication and Organization Processor (COP). The COP is
®
Panther System
Operator's Manual
AW-17791-001 Rev. 002 (EN)
Overview
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