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Overview
This section contains the following information:
Technology Overview
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Panther System Overview
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Panther System Software Overview
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Technology Overview
The Aptima assays are nucleic acid amplification tests that detect DNA or RNA from a
variety of approved specimen types and collection devices according to the appropriate
package insert.
Aptima assays may utilize the following proprietary technologies:
Target Capture—Isolation and purification of nucleic acid from the sample.
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Transcription-Mediated Amplification (TMA
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to produce multiple copies of RNA for easier detection.
Hybridization Protection Assay (HPA)—Detection of the amplified nucleic acid
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using light-emitting nucleic acid probes.
Dual Kinetic Assay (DKA)—Analysis of kinetic chemiluminescent data to
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differentiate the presence of two signals in a single tube.
Real-Time Assays—Assays that measure emission of fluorescence during the TMA
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process.
Internal Controls for Aptima Assays—May be used to control for nucleic acid
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capture, amplification, and detection, as well as operator or instrument error.
Target Capture
The Target Capture process isolates nucleic acid of the target virus and purifies nucleic
acid for amplification.
®
Panther System
Operator's Manual
AW-17791-001 Rev. 002 (EN)
®
)—Amplification of target nucleic acid
Overview
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