Performance Characteristics; Performance Characteristics [Cytoflex] - Beckman Coulter CytoFLEX Series Instructions For Use Manual

System Overview

Performance Characteristics

Minimum
Workstation/
computer
requirements
[CytoFLEX LX]
Compensation
Performance Characteristics

Performance Characteristics [CytoFLEX]

Sensitivity
Fluorescence
resolution
Blue Side scatter
resolution
Violet Side scatter
resolution
Forward and side
scatter resolution
Carryover
Signal acquisition
speed
Carryover
Throughput [With
Plate Loader]
1. This performance characteristic is different if you have the Sample Injection Mode Control Kit installed on your CytoFLEX flow
cytometer. Refer to
1-28
Operating system
Processor
Memory
Storage
Port
USB
Full matrix compensation, manual and automatic.
Novel Compensation Library for storage of spillover values of dyes to easily
determine the correct compensation matrix with new gain settings.
MESF
rCV <3%
The CytoFLEX Flow Cytometer is capable of achieving <3% rCV only with
calibration reagents that have the capability to achieve this specification.
<300 nm
<200 nm
Scatter performance is optimized for resolving lymphocytes, monocytes, and
granulocytes as well as nanoparticles.
Single Tube Loader format
30,000 particles/second with 15 parameters
Performance [With Plate Loader]
Plate Loader format
10 second acquisition without mixing or backflush: <32 min.
1
10 second acquisition with 3 second mixing and 3 second backflush: <45 min.
APPENDIX C, Sample Injection Mode Control
Data Management
Windows® 7, 8, 10 Professional 64-bit
6th Generation Intel Core i7 (8MB cache 4.0 ghz)
8 GB RAM
256 GB
1 GB Ethernet port
5 USB 2.0 and above ports
Performance
FITC: <30 molecules of equivalent soluble
fluorochrome (MESF-FITC) from the 488-nm laser
PE: <10 molecules of equivalent soluble
fluorochrome (MESF-PE) from the 561-nm laser
≤1.0%
<0.5%
Kit.
B49006AH