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Compensation
Creating a Compensation Experiment
NOTE
Figure 6.4
unstained sample.
Figure 6.4 Positive and Negative Populations Without an Unstained Sample
1. Negative population
2. Positive population
5
Record
Select
6
Repeat steps
CAUTION
Risk of erroneous results. While the software automatically adjusts the
compensation calculation according to gain, excessive manual adjustment of the
fluorescence gain could lead to inaccurate results.
7
If necessary, adjust gain while acquiring data from single positive sample tubes. Refer to
Adjusting the Gain
Calculating Compensation Values
1
Check all acquired sample tubes and confirm that the gating is appropriate.
6-8
shows an example of selecting both the positive and negative populations without an
.
1-5
to acquire data from subsequent single positive sample tubes.
in
CHAPTER 5, Data Acquisition and Sample
Analysis.
B49006AH
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Troubleshooting
