Instrument Quality Control And Standardization; Overview - Beckman Coulter CytoFLEX Series Instructions For Use Manual

Overview

This chapter provides information on performing daily quality control (QC) on the CytoFLEX and
CytoFLEX LX flow cytometer and how to confirm that the instrument is working properly within
the specified parameters. Quality control allows you to determine whether your instrument can
provide adequate signal strength and precision.
This chapter also provides information on performing standardization. CytoFLEX Daily QC
Fluorospheres or any other reference material that is relevant for your application(s) may be used
as the standardization sample(s).
Ensure that the standardization sample has been run at optimized experiment settings to determine
the standardization sample threshold setting as well as median values for all relevant channels.
The QC process verifies important system functions. The system:
1. Verifies that the unit hardware configuration matches the default configuration specified in the
software. Refer to
CHAPTER 5, Data Acquisition and Sample
2. Measures the laser power of each individual laser and ensures that each laser meets the system
specifications.
3. Loads the QC sample and begins to acquire data.
4. Verifies that the actual laser delays match those set in the software and will adjust the delay
within certain parameters.
5. Notifies you if laser delay is >2 μs from the previous setting. The software automatically
changes the laser delay setting.
OR
Notifies you if laser delay is >5 μs from the previous setting. Manual laser delay adjustments are
required. Refer to
B49006AH
Instrument Quality Control and
Verifying, Selecting, Editing, and Creating Detector Configuration
Analysis.
Setting Laser Delay in CHAPTER 11, Replacement/Adjustment
CHAPTER 4
Standardization
in
Procedures.
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