Beckman Coulter CytoFLEX Series Instructions For Use Manual page 278

Troubleshooting
Troubleshooting Table
Table 9.1 Troubleshooting (Continued)
Problem
The fluid status
information displays red
for Sheath and/or Waste
even though the sheath
fluid container is full and
the waste container is
empty.
The sample tube holder
cannot move up and
down automatically.
The sample flow rate is
unstable.
9-12
Probable Cause
• Instrument data connection
error.
• The sensor connection is not
working properly.
• The sensor does not work
properly.
• The sheath fluid/waste
harnesses have been
secured on the wrong
container.
The setting is incorrect.
• The sample probe is clogged.
• The sample contains
aggregates or clumps.
• There are air bubbles in the
flow cell.
• The sample peristaltic pump
tubing is aged.
• The sample peristaltic pump
tubing is not properly
connected.
Corrective Action
1. Ensure the sheath fluid/waste harnesses
are secured to the correct container.
2. Ensure that the USB data cable is
securely connected to the back of the
Cytometer and the back of the
Workstation. Refer to Figure
3. Restart the software.
4. Ensure the sheath fluid harness and/or
the waste harness are properly
connected.
5. Verify that the float of the sensor in the
sheath fluid container and/or waste
container moves freely.
6. Replace the sheath fluid harness and/or
the waste harness. Refer to
the Sheath Fluid Harness and/or Waste
Harness in CHAPTER 11, Replacement/
Adjustment Procedures.
7. If the problem persists,
1. Ensure that the sample injection mode in
the software is in Semi-Automatic
Injection mode. Refer to
Proper Sample Injection Mode
CHAPTER 3, Daily Startup.
2. If the problem persists,
1. Run Prime. Refer to
Priming the Flow Cell
in CHAPTER 11, Replacement/
Adjustment Procedures.
2. Run Daily Clean. Refer to
CHAPTER 10, Cleaning Procedures.
3. Clean the sample probe. Refer to
Cleaning the Sample Probe
CHAPTER 10, Cleaning Procedures.
4. Filter the sample using an appropriately
sized mesh aperture filter.
5. Ensure that the sample tubing is properly
connected. Refer to Replacing the
Sample Probe and/or the Sample
Peristaltic Pump Tubing
Replacement/Adjustment
6. Replace the sample probe and sample
peristaltic pump tubing. Refer to
Replacing the Sample Probe and/or the
Sample Peristaltic Pump Tubing
CHAPTER 11, Replacement/Adjustment
Procedures.
7. If the problem persists,
1.17.
Replacing
contact us.
Selecting the
in
contact us.
Daily Clean in
in
in CHAPTER 11,
Procedures.
in
contact us.
B49006AH