Beckman Coulter CytoFLEX Series Instructions For Use Manual page 279

Table 9.1 Troubleshooting (Continued)
Problem
The sampling flow rate is
too fast.
Laser power is low.
Populations are drifting.
B49006AH
Probable Cause
• The threshold setting is too
low.
• The sample concentration is
too high.
• There are too many sample
fragments.
• The sheath fluid filter is
clogged.
• The sample flow rate
requires calibration.
Communication error
• Air bubbles are in the flow
cell.
• Air bubbles are in the
system.
• The sheath fluid harness float
is restricted.
Troubleshooting Table
Corrective Action
1. Use the manual threshold setting to
increase the threshold. Refer to
the Threshold in CHAPTER 5, Data
Acquisition and Sample
2. Calibrate the sample flow rate. Refer to
Calibrating the Sample Flow Rate
Calibrating the Sample Flow Rate [With
Plate Loader] in CHAPTER 11,
Replacement/Adjustment
3. Dilute the sample and adjust the
concentration to approximately 10
4. Filter the sample using an appropriately
sized mesh aperture filter.
5. Restain the sample.
6. Replace the Sheath Fluid Filter. Refer to
Replacing the Sheath Fluid Filter
CHAPTER 11, Replacement/Adjustment
Procedures.
7. If the problem persists,
1. Reinitialize. Refer to
Initializing the
Instrument in CHAPTER 3, Daily
2. Restart the Cytometer.
3. If the problem persists,
1. Run Prime. Refer to
Priming the Flow Cell
in CHAPTER 11, Replacement/
Adjustment Procedures.
2. Ensure that the sheath fluid harness and/
or waste harness is not kinked.
3. Ensure that the sheath fluid harness and/
or waste harness is securely connected.
4. Run Prime. Refer to
Priming the Flow Cell
in CHAPTER 11, Replacement/
Adjustment Procedures.
5. Verify that the float of the sensor in the
sheath fluid container moves freely.
6. Replace the sheath fluid harness. Refer
to Replacing the Sheath Fluid Harness
and/or Waste Harness in
Replacement/Adjustment
7. If the problem persists,
Troubleshooting 9
Adjusting
Analysis.
or
Procedures.
6
/mL.
in
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Startup.
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CHAPTER 11,
Procedures.
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9-13