Preparing The Qc Sample; Required Materials; Preparation Process Cytoflex Daily Qc Fluorospheres; Preparation Process Cytoflex Ir Daily Qc Fluorospheres - Beckman Coulter CytoFLEX Series Instructions For Use Manual

Preparing the QC Sample

Required Materials

The following materials are required to complete the QC process:
• CytoFLEX Daily QC Fluorospheres
• CytoFLEX Sheath Fluid or another nonionic sheath fluid
• Sample tubes (12 x 75 mm).
• Vortexer
• CytoFLEX IR Daily QC Fluorospheres (for systems configured with an IR laser)

Preparation Process CytoFLEX Daily QC Fluorospheres

1
Take one sample tube and label it as the QC sample tube.
2
Add approximately 1 mL of deionized water to the sample tube.
3
Use the vortexer or shake vigorously to thoroughly mix the bottle of CytoFLEX Daily QC
Fluorospheres.
4
Add three drops of CytoFLEX Daily QC Fluorospheres to the sample tube.
5
Vortex the sample tube until a uniform mixture is achieved.
6
Place the sample tube in a dark location at 2-8 C until ready to load the tube into the
instrument for QC.
NOTE
Tubes containing diluted CytoFLEX Daily QC Fluorospheres should be stored and sealed in a dark
location at 2-8 C for up to 5 days.

Preparation Process CytoFLEX IR Daily QC Fluorospheres

1
Take one sample tube and label it as the QC/IR sample tube.
2
Add approximately 1 mL of deionized water to the sample tube.
B49006AH
Instrument Quality Control and Standardization
Preparing the QC Sample
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