Labware Material Compatibility With Solvents And Sample; Characteristics And Chemical Resistances Of Tube And Bottle - Beckman Coulter Avanti J Series Instructions For Use Manual

Tubes, Bottles, and Accessories
Labware Selection Criteria
Table 2.1 Characteristics and Chemical Resistances of Tube and Bottle Materials
thinwall transparent
polypropylene
thickwall translucent
polypropylene
Ultra-Clear transparent
polycarbonate transparent
polypropylene translucent/
transparent
polyethylene transparent/
translucent
cellulose transparent
propionate
stainless steel opaque
S = satisfactory resistance
a. Refer to Appendix A for information about specific solutions.
b. Polypropylene and cellulose propionate tubes with diameters of 5 to 13 mm may be sliced using the Centritube Slicer (part number 347960)
and appropriate adapter plate.
NOTE
This information has been consolidated from a number of sources and is provided only as a guide to
the selection of tube or bottle materials. Soak tests at 1 g (at 20°C) established the data for most of the
materials; reactions may vary under the stress of centrifugation, or with extended contact or temperature
variations. To prevent failure and loss of valuable sample, ALWAYS TEST SOLUTIONS UNDER OPERATING
CONDITIONS BEFORE USE.
WARNING
Do not use flammable substances in or near operating centrifuges.

Labware Material Compatibility with Solvents and Sample

The chemical compatibility of tube or bottle materials with the gradient-forming medium or other
chemicals in the solution is an important consideration. Although neutral sucrose and salt solutions
cause no problems, alkaline solutions cannot be used in Ultra-Clear tubes or in polycarbonate tubes
and bottles. Polycarbonate and Ultra-Clear tubes are incompatible with DMSO, sometimes used in
the preparation of sucrose gradients for sedimentation of denatured DNA.
2-2
yes yes no S U
no b yes S S
no
yes yes no S U
no no yes M U
no b yes S S
no
yes no yes S S
no b no S U
no
no no yes S U
M = marginal resistance
a
U M S U U
S M S M M
U S U U U
U M U U U
S M S M S
S S S S U
U U U M S
S S M S S
U = unsatisfactory resistance
U U U S
U M U S
U U U M
U U M M
M M M S
M M M S
S U M S
S M S M
PN JR-IM-10AG