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Adjusting the Threshold
By adjusting the threshold, the user can remove unnecessary signal noise to ensure that most of the
data collected consists of desired signal data. After the threshold settings have been configured for
a given channel, the acquisition of data from this channel will only be triggered by signals that
exceed the established threshold. Threshold settings have considerable bearing on whether the
appropriate events can be acquired.
1
Create a plot to view the channels where the threshold will occur. Generally, a bivariate plot
showing FSC and SSC is used.
NOTE
Threshold can be defined for any of the fluorescence channels.
2
Select
3
Select the
B49006AH
on the left side of the screen.
Threshold
tab in the Acq. Setting window.
Data Acquisition and Sample Analysis
Configuring Acquisition Settings
5
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Table of Contents
Troubleshooting
