Beckman Coulter CytoFLEX Series Instructions For Use Manual page 281

Table 9.1 Troubleshooting (Continued)
Problem
No data acquisition.
Data populations are
normal on one laser, but
too low on another laser.
B49006AH
Probable Cause
• The threshold setting is too
high.
• The gain setting is too low.
• Sheath fluid flow is
insufficient.
• Laser power is insufficient.
• The sample probe is clogged.
The laser delay setting is
incorrect.
Troubleshooting Table
Corrective Action
1. Decrease the threshold setting. Refer to
Adjusting the Threshold
Data Acquisition and Sample
2. Increase the gain setting. Refer to
Adjusting the Gain in CHAPTER 5, Data
Acquisition and Sample
3. Ensure that the sheath fluid harness and/
or waste harness is not kinked.
4. Ensure that the sheath fluid harness and/
or waste harness is securely connected.
5. Run Prime. Refer to
Priming the Flow Cell
in CHAPTER 11, Replacement/
Adjustment Procedures.
6. Reinitialize. Refer to
Initializing the
Instrument in CHAPTER 3, Daily
7. Restart the Cytometer.
8. Verify that your sample does not have
excessive debris. If it does:
a. Filter the sample using an
appropriately sized mesh aperture
filter.
b. Restain the sample.
9. Clean the sample probe. Refer to
Cleaning the Sample Probe
CHAPTER 10, Cleaning Procedures.
10. Replace the sample probe and the
sample peristaltic pump tubing. Refer to
Replacing the Sample Probe and/or the
Sample Peristaltic Pump Tubing
CHAPTER 11, Replacement/Adjustment
Procedures.
11. If the problem persists,
1. Ensure that the laser delay is set
correctly. Refer to Setting Laser Delay
CHAPTER 11, Replacement/Adjustment
Procedures.
2. If the problem persists,
Troubleshooting 9
in CHAPTER 5,
Analysis.
Analysis.
Startup.
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in
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9-15