All Gates - Example; Circular Gating Logic - Example - Beckman Coulter CytoFLEX Series Instructions For Use Manual

Flow cytometers
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Data Acquisition and Sample Analysis
Configuring Acquisition Settings
3
Select the gates to display.
a. Select the heading area of the plot, select the parent population/gate to display in the plot
from the drop-down menu. The selected parent gate appears in the tab area of the plot.
NOTE
5-46
The CytExpert software will not list gates which would create circular gating logic.
Figure 5.2
shows all gates defined in the example experiment below. Note that the only gate
option in plot 1 of
Figure 5.3
Plot 1 cannot be gated on P1 because P1 is on that plot.
Plot 1 cannot be gated on P2 because P2 is gated on P1.
Plot 1 cannot be gated on the P2 OR P1 combo population because the gate logic contains
P1.
Figure 5.2 All Gates - Example Experiment
Figure 5.3 Circular Gating Logic - Example Experiment
is P2 for the following reasons:
B49006AH

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