Beckman Coulter Cell Lab Quanta SC Manuals

Beckman Coulter Cell Lab Quanta SC Manuals

Manuals and User Guides for Beckman Coulter Cell Lab Quanta SC. We have 1 Beckman Coulter Cell Lab Quanta SC manual available for free PDF download: Instructions For Use Manual

Beckman Coulter Cell Lab Quanta SC Instructions For Use Manual

Beckman Coulter Cell Lab Quanta SC Instructions For Use Manual (202 pages)

Brand: Beckman Coulter | Category: Laboratory Equipment | Size: 13 MB

Table of Contents

Revision Status

3
- Table of Contents
  5
Contents

6
- Illustrations
  11
- Tables
  13
Introduction

15
- Options
  15
- Graphic Conventions
  16
- Text Conventions
  16
- Reagents
  17
- Safety Symbols
  17
1 Use and Function

19
- System Overview
  19
- Intended Use
  19
- Quanta Sc System Overview
  20
- Quanta SC System
  20
- Schematic Overview of Quanta
  20
  - Bottle
    20
  - System Components
    21
  - Principles of Operation
    22
- Triangular Flow Cell
  22
  - Warning Labels and Precautions
    23
    
    Mercury Arc Lamp
    23
- Mercury Arc Lamp Warning
  23
  - Laser
    24
- Laser Warning Labels
  25
  - Laser Interlocks
    26
  - Handling Precautions
    27
  - Options
    27
    
    Hardware Options
    27
- Optical Filter Configuration -- Laser
  27
  - Flow Cell Configurations
    28
  - Jumper Positions for Different Size Particles ΜM
    29
  - Light Source Configuration
    29
  - Printer
    29
  - Reagents
    29
  - List of Approved Reagents
    29
  - Controls and Indicators
    30
- Vacuum Regulator: Controls and 1.13 Jumper Positions: Controls and
  30
  - Waste Bottle and Vacuum Bottle
    31
- Reservoirs: Controls and 1.15 Waste System and
  31
- Workstation PC: Controls and 5.1 Desktop
  32
  - Material Safety Data Sheets (Msds)
    33
2 Installation

35
- Delivery Inspection
  35
- Special Requirements
  35
  - Space and Accessibility
    35
  - Installation Category
    36
  - System Dimensions and Accessibility
    36
  - Electrical Input
    37
  - Ambient Temperature and Humidity
    37
  - Heat Dissipation
    37
  - Drainage
    38
- Installation Procedures
  38
  - Interunit Connections
    38
  - Lifting and Carrying
    38
3 Operation Principles

39
- Overview
  39
- Sample Flow
  39
- Software
  39
4 Specifications

41
- Performance Specifications
  41
  - Absolute Count
    41
  - Fluorescence
    41
  - Sizing
    41
  - Optics
    41
  - Absolute Count Performance Specifications
    41
  - Fluorescence Performance Specifications
    41
  - Sizing Performance
    41
  - Optics Performance
    41
  - Parameters
    42
  - Calculated Parameters
    42
    
    FC and FSD Relevance
    42
    
    Mathematical Definitions
    42
    
    Numeric Range of FSD and FC
    42
    
    FSD and FC Calibration
    43
    
    Possible FSD and FC Calculation Errors
    43
  - Software
    43
  - Fluidics
    43
  - Fluidics Specifications
    43
  - Installation Requirements
    44
- Cups
  44
- Flow Cell
  44
- Cups Approved for Use
  44
5 Getting Started

45
- Powering up the System
  45
  - Turn on the Instrument and the Computer
    45
- Introduction to the Quanta Sc Software
  45
  - Overview
    45
  - Microsoft Windows Basics
    46
    
    Desktop
    46
    
    Windows Password
    46
  - Launching the Software
    47
    
    To Launch from the Desktop
    47
    
    To Launch from the Start Menu
    47
  - Main Screen
    48
    
    Main Menu
    48
  - Menu Options
    48
    
    File Menu
    49
    
    Instrument Menu
    49
    
    Analysis Menu
    50
    
    Gain Menu
    50
    
    Volume Menu
    50
    
    Compensation Screen
    51
    
    Regions Screen
    51
    
    Current Settings Screen
    52
    
    Protocols Screen
    52
- Current Instrument Settings
  52
  - File Information Screen
    53
  - Security Menu
    53
  - Help Menu
    54
  - Closing a Window or Closing the Software
    54
    
    Closing a Window
    54
    
    Closing the Software
    54
  - Powering down the System
    55
    
    Turn Instrument and Workstation off
    55
  - Placing a Cup on the Instrument
    56
6 Daily Routine

59
- Perform Startup
  59
- Run Qc (Quality Control) Samples
  62
- Perform Shutdown
  62
7 Software

67
- Understanding the Main Screen
  67
- Main Screen: Defined
  67
- Main Menu
  68
  - Parameter Information
    69
- Main Screen: Parameter
  69
  - Calibrate FSD or FC
    70
- Main Screen: Calibrate
  70
  - Region Statistics
    71
  - Working with the File Menu
    71
    
    Auto Save Option
    72
    
    Saving Listmode (.LMD) Files
    72
    
    Playing Back Files
    73
    
    Defining Playback Options
    74
    
    Exporting Data to Excel
    75
    
    Generating a Microsoft Excel Report
    75
    
    Printing
    75
    
    Exiting (Closing) the Application
    77
  - Working with the Instrument Menu
    77
    
    Startup
    78
    
    Shutdown
    79
    
    Defining the Power Setting
    80
    
    Running the Cleaning Cycle
    82
  - Flushing
    83
  - Fill Cup
    84
  - Resetting Fluid Count
    84
  - Setting up the Instrument
    85
    
    Understanding the Instrument Setup Screen
    86
- Instrument Setup Screen
  86
- Import Setting Screen
  91
  - Understanding the Auto Optical Alignment Screen
    92
- Auto Optical Alignment Screen
  92
- Working with the Gain Menu
  93
  - Show Gain Settings
    93
  - Defining Tracking Settings
    94
    
    Understanding the Tracking Settings Screen
    95
- Tracking Settings Screen
  95
  - Tracking Start
    96
  - Reset Tracking
    97
- Working with the Volume Menu
  97
  - Calibrating the Volume
    97
    
    Calibration Beads
    98
    
    Understanding the Volume Calibration Screen
    101
  - Displaying Channels
    101
- Volume Calibration Screen
  101
- Working with the Analysis Menu
  102
  - Parameter Ratio Analysis
    102
  - Data Flag Settings
    103
- Working with the Regions Menu
  105
  - Managing Regions
    105
    
    Understanding the Manage Regions Screen
    108
- Manage Regions Screen
  108
- Single Parameter Region Definition Screen
  109
- Defined
  109
- Region Definition Screen for And/Or Regions
  110
- Defined
  110
  - Showing Region Statistics
    111
    
    Understanding the Region Statistics Screen
    112
- Region Statistics Screen
  112
- Working with the Compensation Menu
  113
  - Defining Compensation Settings
    113
    
    Understanding the Compensation Settings Screen
    116
- Compensation Settings Screen
  116
- Defined
  117
- Working with the Manage Protocols Screen
  118
  - Understanding the Protocol Management Screen
    119
  - Loading Protocols
    120
  - Creating New Protocols
    121
  - Saving Current Settings to a Protocol
    123
  - Deleting Protocols
    124
  - Save Description Changes (Editing a Protocol)
    126
  - Create/Delete Protocol Groups
    127
- Assign a User to a Protocol Group
  
  128
  - Remove a User Assigned to a Protocol Group
    129
  - 7.10 Working with the Current Settings Screen
    130
    
    Defining Stop Sample Criteria
    130
    
    Defining the Concentration (Use, # of Seconds, and Start Time)
    131
    
    Enabling/Disabling Checkboxes
    131
    
    Defining Auto Save Options
    132
    
    Creating/Modifying Text in Excel Report
    134
    
    Customizing an Excel Report
    134
    
    Pre-Defined Excel Templates
    134
    
    Defining Filter Configurations
    136
    
    Understanding the Current Instrument Settings Screen
    137
  - 7.11 Working with the File Information Screen
    139
    
    Entering Sample Information
    140
    
    Understanding the File Information Screen
    141
  - File Information
    141
    
    7.12 Working with the Security Menu
    142
    
    Change Password
    142
    
    Manage Users
    143
    
    Understanding the Security Menu Screen
    144
    
    Modify Database Path
    145
    
    Password Options
    145
    
    7.13 Working with the Help Menu
    146
    
    Launching Help
    146
    
    Viewing Software Information
    146
8 Quality Control

147
- Overview
  147
- Qc Materials
  147
- Daily Qc
  147
- Qc Method
  150
  - Prepare QC Material
    150
    
    Flow-Check Fluorospheres
    150
- Automatically Aligning the Optics
  150
  - Arc Lamp Alignment Beads
    150
9 Sample Analysis

155
- Before Running Samples
  155
- Preparing Samples
  155
- Running Samples
  156
- After Running Samples
  158
- Setting Initial Gain, Voltage and Discriminator Values
  159
- Working with Regions
  159
10 Cleaning/Replacement

161
- 10.1 Instrument Cleaning and Handling Requirements
  161
- 10.2 Instrument Quick Disconnect
  161
- 10.3 Opening/Closing the Cover
  162
- 10.4 Emptying the Waste Bottle
  164
- 10.5 Changing a Filter
  165
- 10.6 Changing an Arc Lamp Excitation Filter
  172
- 10.7 Replacing the Fuse
  175
- 10.8 Adjusting Fuse Voltage
  177
11 Troubleshooting

181
- 11.1 System Connections
  181
- 11.2 Monitor Vacuum Readings
  183
- 11.3 Error Messages
  184
- 11.4 Troubleshooting Guide
  185
- Troubleshooting Guide
  186
Glossary

189
Beckman Coulter, Inc. Customer End User License Agreement

199

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