Page 1 ® ® ™ COULTER EPICS Flow Cytometer ® ® ™ COULTER EPICS XL-MCL Flow Cytometer Service Manual Coulter Epics XL • MCL PN 4237029F (October 2002) Beckman Coulter, Inc. Fullerton, CA 92835...
Page 2: Warnings And Precautions
IMPORTANT - Can cause misleading results. Beckman Coulter, Inc. urges its customers to comply with all national health and safety standards such as the use of barrier protection. This may include, but it is not limited to, protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or any other automated laboratory analyzer.
Page 3: Revision Status
REVISION STATUS Initial Issue, 7/93 Software Version 1.0. Revision B, 6/94 Software Version 1.5. Added fluidic schematics, electronic block diagrams, and procedures, and updated parts for MCL. Pages changed or added: i, iii to vii, 1-1, 2-6 to 2-19, 4-14 to 4-35, 5-1, 5,7, 5-8, 8-1 to 8-6, 8-11 to 8-16. Revision C, 10/94 Software Version 1.5.
Page 4 REVISION STATUS PN 4237029F...
Page 5: Table Of Contents
CONTENTS WARNINGS AND PRECAUTIONS REVISION STATUS, iii CONTENTS, v INTRODUCTION, 1.1-1 MANUAL DESCRIPTION, 1.1-1 Scope, 1.1-1 Notification of Updates, 1.1-1 Intended Audience, 1.1-1 Organization, 1.1-2 Numbering Format, 1.1-3 Special Headings, 1.1-3 WARNING, 1.1-4 CAUTION, 1.1-4 IMPORTANT, 1.1-4 ATTENTION, 1.1-4 Note, 1.1-4 Conventions, 1.1-4 SAFETY PRECAUTIONS, 1.2-1...
Page 6 CONTENTS Data Acquisition Card Cage, 2.2-4 Amplifier (Amp)/Signal Conditioner Card, 2.2-6 Trans Data Acquisition Card, 2.2-8 System Interface Card, 2.2-9 Cyto Transputer Card, 2.2-10 MCL Interface Card, 2.2-11 Optical Transprocessor Extended Memory Cards, 2.2-12 Opto Transprocessor EXMEM Card, 2.2-12 Opto Transprocessor EXMEM II Card, 2.2-12 Transputers, 2.2-12 Fiber Optic Interface...
Page 7 CONTENTS PREINSTALLATION CHECKS, 3.2-1 Pre-Site Inspection Compliance, 3.2-1 Education Center Training, 3.2-1 Condition of Cartons Received, 3.2-1 Optional Computer Peripherals, 3.2-1 Supplies, 3.2-1 INITIAL INSTALLATION, 3.3-1 Tools/Supplies Needed, 3.3-1 Unpacking, 3.3-1 Power Supply Module, 3.3-1 Cytometer, 3.3-1 Power Supply Installation, 3.3-2 Cytometer Installation, 3.3-3 Remove Covers to Provide Needed...
Page 8 CONTENTS Power Cords, 3.5-5 Connect Tubings, 3.5-5 Prepare the Waste Container for Tubing Connection, 3.5-5 Tubing Connections, 3.5-6 SOFTWARE INSTALLATION, 3.6-1 Tools/Supplies Needed, 3.6-1 SYSTEM II Software Installation, 3.6-1 OPERATIONAL CHECKS, 3.7-1 Tools/Supplies Needed, 3.7-1 Preliminary Operational Checks, 3.7-1 Purge Air Bubbles from the Sheath Filter, 3.7-1 Initial System Verification, 3.7-3...
Page 9 CONTENTS ™ 3.10 FlowCentre II MULTIMEDIA WORKSTATION UPGRADE, 3.10-1 Purpose, 3.10-1 Tools/Supplies Needed, 3.10-1 Preparation, 3.10-1 Opto Transprocessor EXMEM or EXMEM II Card Retrieval and Installation, 3.10-1 Mouse Connection, 3.10-1 Keyboard Connection, 3.10-2 Monitor Connection (New or Existing), 3.10-2 Cable Connections, 3.10-3 Fiber Optic Interface Cables, 3.10-3...
Page 10 CONTENTS ® 3.15 LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 8.0, 3.15-1 Purpose, 3.15-1 Tools/Supplies Needed, 3.15-1 Preparation, 3.15-1 Hardware Installation, 3.15-1 CD-ROM Usage in DOS, 3.15-1 Software Installation, Client Setup, 3.15-1 Software Installation, Server Setup, 3.15-5 Software and Hardware Validation, 3.15-5 ®...
Page 11 CONTENTS Procedures, 4.1-2 Tools and Supplies, 4.1-3 Instrument Performance Verification, 4.1-3 Power Down / Power Up Using SYSTEM II™ Software, 4.1-3 Power Down Using SYSTEM II Software, 4.1-3 Stage 1: Power Off the Cytometer (system using SYSTEM II software), 4.1-3 Stage 2: Power Off the Workstation (system using SYSTEM II software), 4.1-4 Stage 3: System Power Down (system using SYSTEM II...
Page 12 CONTENTS Procedure 1, 4.3-5 How to Remove and Reinstall the Cytometer Top Cover, 4.3-5 Tools/Supplies Needed, 4.3-5 Removing the Cytometer Top Cover, 4.3-5 Cytometer Interlock Switch, 4.3-6 Reinstalling the Cytometer Top Cover, 4.3-6 Procedure 2, 4.3-7 How to Remove and Reinstall the Cytometer Right-Side Cover, 4.3-7 Tools/Supplies Needed, 4.3-7...
Page 13 CONTENTS Procedure 9, 4.3-23 How to Open and Close the MCL Door, 4.3-23 Tools/Supplies Needed, 4.3-23 Opening the MCL Door, 4.3-23 MCL Interlock Switch, 4.3-23 Closing the MCL Door, 4.3-24 Procedure 10, 4.3-25 How to Unlatch and Relatch the MCL Covers, 4.3-25 Tools/Supplies Needed, 4.3-25...
Page 14 CONTENTS Procedure 17, 4.3-46 How to Remove and Reinstall the Lower Pneumatics Drawer, 4.3-46 Tools/Supplies Needed, 4.3-46 Removing the Lower Pneumatics Drawer on a Cytometer with the MCL Option Installed, 4.3-46 Removing the Lower Pneumatics Drawer on a Cytometer without the MCL Option, 4.3-49 Reinstalling the Lower Pneumatics Drawer, 4.3-50...
Page 15 CONTENTS OPTICAL ALIGNMENT, 4.6-1 Purpose, 4.6-1 Tools/Supplies Needed, 4.6-1 Preparation, 4.6-1 Laser Beam Steering, 4.6-1 Forward Scatter (FS) Mask Alignment, 4.6-2 How to Proceed, 4.6-4 SAMPLE AND SHEATH SENSOR CALIBRATION, 4.7-1 Purpose, 4.7-1 Tools/Supplies Needed, 4.7-1 Preparation, 4.7-1 Preliminary Voltage Checks, 4.7-1 Calibration, 4.7-2 Operational...
Page 16 CONTENTS Removal, 4.11-1 Front Segmenting Pad, 4.11-1 Middle Segmenting Pad, 4.11-2 Rear Segmenting Pad, 4.11-2 Clean the Shaft, 4.11-3 Installation, 4.11-3 Rear Segmenting Pad, 4.11-3 Middle Segmenting Pad, 4.11-3 Front Segmenting Pad, 4.11-4 Complete the Installation, 4.11-4 Reinstall Covers, 4.11-4 Verification, 4.11-4 4.12 MCL OPTION FIELD ADJUSTMENT, 4.12-1...
Page 17 CONTENTS If Calibrating Without Replacing the Amp/Signal Conditioner Card, 4.15-2 Calibration, 4.15-2 Verify Acceptable Histograms, 4.15-4 Reinstall Cover, 4.15-6 System Verification, 4.15-6 4.16 PREPARING INSTRUMENT FOR SHIPMENT, 4.16-1 Purpose, 4.16-1 Tools/Supplies Needed, 4.16-1 Procedure, 4.16-1 4.17 INSTRUMENT SHUTDOWN, 4.17-1 Purpose, 4.17-1 Tools/Supplies Needed, 4.17-1 Procedure, 4.17-1...
Page 18 CONTENTS MCL Power Supply, 4.20-6 Preparation, 4.20-6 Voltage Verification, 4.20-6 Voltage Adjustment, 4.20-7 4.21 RE-IMAGING FlowCentre™ COMPUTERS, 4.21-1 Purpose, 4.21-1 Tools/Supplies Needed, 4.21-1 Preparation, 4.21-1 Re-imaging a FlowCentre II Tower Computer, 4.21-1 Re-imaging a FlowCentre Desktop Computer without a Cache Controller Card, 4.21-1 Re-imaging a FlowCentre Desktop Computer with a Cache Controller Card, 4.21-2...
Page 19 CONTENTS PREVENTATIVE MAINTENANCE INSPECTION (PMI), 5.2-1 Purpose, 5.2-1 Tools/Supplies Needed, 5.2-1 Procedure, 5.2-1 System Verification, 5.2-2 SCHEMATICS, 6.1-1 ENGINEERING SCHEMATICS, 6.1-1 TROUBLESHOOTING, 7.1-1 LEVEL SENSE INDICATORS AND ERROR MESSAGES, 7.1-1 Level Sense Indicators, 7.1-1 Cleanse Low, 7.1-1 Sheath Low and Waste Full, 7.1-1 Error Messages, 7.1-1...
Page 20 CONTENTS DMA Acquisition - Not Used, 7.2-4 Front Panel Test, 7.2-4 Purpose, 7.2-4 Circuit Cards Checked in this Test, 7.2-5 Response if Test Fails, 7.2-5 General Information, 7.2-5 Grand Canyon Adjust, 7.2-6 Purpose, 7.2-6 Circuit Cards Checked in this Test, 7.2-6 Response if Test Fails, 7.2-6 Grand Canyon...
Page 21 CONTENTS MCL Manual Control, 7.2-12 Purpose, 7.2-12 Sensor Status, 7.2-12 Controls, 7.2-12 MCL Mix Test, 7.2-12 Purpose, 7.2-12 MCL Pneumatic Status, 7.2-12 Purpose, 7.2-12 Sensor Status, 7.2-12 Response if Test Fails, 7.2-12 MCL POWER UP Status, 7.2-13 Purpose, 7.2-13 Response if Test Fails, 7.2-13 MCL Probe Align, 7.2-13...
Page 22 CONTENTS PMT Voltage Control, 7.2-21 Purpose, 7.2-21 Circuit Cards Checked in this Test, 7.2-21 Response if Test Fails, 7.2-21 Pneumatic Sensor Test, 7.2-21 Purpose, 7.2-21 Circuit Cards Checked in this Test, 7.2-21 Response if Test Fails, 7.2-22 Pulse RAMP Test, 7.2-22 Purpose, 7.2-22 Components Checked in this Test, 7.2-22...
Page 23 CONTENTS VME Data Bus Test, 7.2-26 Purpose, 7.2-26 Circuit Cards Checked in this Test, 7.2-27 Response if Test Fails, 7.2-27 Waste Chamber Full Test, 7.2-27 Purpose, 7.2-27 Components Checked in this Test, 7.2-27 Response if Test Fails, 7.2-27 XY Display - Not Used, 7.2-27 PARTS LISTS, 8.1-1...
Page 24 CONTENTS Cyto Transputer Card, A.2-7 Circuit Card Location in the Cytometer, A.2-7 Component Locations, A.2-7 Jumpers, A.2-7 Switches, A.2-7 Fiber Optic Interface Card, A.2-8 Circuit Card Location in the Cytometer, A.2-8 Component Locations, A.2-8 Jumpers, A.2-8 Switches, A.2-8 Front Panel LED and Switch Input Card, A.2-9 Circuit Card Location in the Cytometer, A.2-9...
Page 25 CONTENTS Switches, A.2-16 Power Module Control II Card (EMC Version), A.2-17 Circuit Card Location in the Power Supply Module, A.2-17 Component Locations, A.2-17 Jumpers, A.2-17 Switches, A.2-17 Sensor Card, A.2-18 Circuit Card Location in the Cytometer, A.2-18 Component Locations, A.2-18 Jumpers, A.2-18 Switches, A.2-18 Solenoid Power Distribution...
Page 26 CONTENTS CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS, A.5-1 Overview, A.5-1 Main Cytometer Components, A.5-1 Locating a Component, A.5-1 Cytometer Anchor Illustration, A.5-2 Components Accessed with the Cytometer Top Cover Removed, A.5-5 Data Acquisition Card Cage, A.5-7 Data Acquisition Card Cage, Component Locations, A.5-7 Optical Collection Area, A.5-13...
Page 27 CONTENTS DEFAULT Settings for the System BIOS, B.1-2 Standard CMOS Setup, B.1-2 Advanced CMOS Setup, B.1-2 Advanced Chipset Setup, B.1-3 Power Management Setup, B.1-3 PCI / Plug and Play Setup, B.1-4 Peripheral Setup, B.1-5 WINDOWS 98 Configuration from Device Manager, B.1-6 Control Panel Setup, Device Manager, Computer, B.1-6 CONFIG.SYS...
Page 28 CONTENTS ® Sybase SQL ANYWHERE™ MINIMUM REQUIREMENTS, B.5-1 Client Requirements, B.5-1 Database Server Requirements, B.5-1 Network Requirements, B.5-1 FIELD WORKSHEETS, C.1-1 WORKSHEETS, C.1-1 Field Engineer Worksheet, C.1-1 Network Configuration Worksheet, C.1-2 EXAMPLES OF SETUP FILES, D.1-1 CLIENT FLOW CYTOMETER, D.1-1 Config.sys, D.1-1 Autoexec.bat, D.1-1 Startnet.bat, D.1-1...
Page 29 CONTENTS Hand-Held Bar-Code Scanner Setup Parameters, F .3-1 Default Configurations, F .3-1 General Parameters, F .3-2 Serial Communication Parameters, F .3-2 Symbologies, F .3-2 Wand Emulation Parameters, F .3-3 Keyboard Wedge Parameters, F .3-3 Memory Module Set Up, F .3-3 Supplemental Programming Symbols, F .3-3 F .4...
Page 30 CONTENTS ILLUSTRATIONS 1.2-1 Laser Warning Labels - Cytometer, Top and Side, 1.2-4 1.2-2 Laser Warning Labels - Cytometer, Sensing Compartment Cover, 1.2-4 1.2-3 Laser Warning Labels - Cytometer, Optical Area, Front View, 1.2-5 1.2-4 Laser Warning Labels - Cytometer, Optical Area, Side View, 1.2-5 1.2-5 Laser Certification Label - Cytometer,...
Page 31: Locations
CONTENTS 3.3-15 Remove the Plastic Shipping Inserts from the Segmenting Valve, 3.3-13 3.3-16 Sheath Filter Location and Associated Components, 3.3-15 3.4-1 Typical Workstation Setup, 3.4-1 3.4-2 Electronic Cable Connections - Mouse and Keyboard, 3.4-2 3.4-3 Connecting the 17-inch Flat Panel TFT Monitor Cables, 3.4-3 3.5-1 Instrument...
Page 32: Open
CONTENTS 4.3-29 Left-Side Cover Removal or Installation (Cytometer without MCL Option), 4.3-36 4.3-30 Use a Long Screwdriver to Brace the Upper Cover Open, 4.3-40 4.3-31 Filter Shield Screw Locations, 4.3-40 4.3-32 Data Acquisition Card Cage Locked in its Vertical Position, 4.3-44 4.3-33 Removing the MCL from the Cytometer Frame, 4.3-48 4.3-34 Removing the Lower Pneumatics Drawer...
Page 33: View
CONTENTS 8.2-14 Upper Pneumatics Drawer (See Table 8.2-14), 8.2-26 8.2-15 VL22 - Exploded View of Upper Valve (See Table 8.2-15), 8.2-28 8.2-16 Bracket and Fittings - Upper Pneumatics Drawer (See Table 8.2-16), 8.2-29 8.2-17 VL21 - Exploded View of Lower Valve (See Table 8.2-17), 8.2-30 8.2-18 Quick Disconnects Below Upper Pneumatics Drawer (See Table 8.2-18), 8.2-31...
Page 34 CONTENTS 8.2-52 System Pressure Adjustment Knob and Related Components (See Table 8.2-52), 8.2-86 8.2-53 Vacuum and Air Filters with Related Components (See Table 8.2-53), 8.2-87 8.2-54 System Vacuum Gauge and Related Components (See Table 8.2-54), 8.2-88 8.2-55 Power Supply Module - Right Side with Cover Removed (See Table 8.2-55), 8.2-89 8.2-56 Waste Container (See Table...
Page 35 CONTENTS A.5-7 Inside View of the Cytometer with the Filter Shield Removed (See Table A.5-7), A.5-24 A.5-8 View of an Open Reagent Drawer (See Table A.5-8), A.5-27 A.5-9 Rear Panel Components, Exterior View (See Table A.5-9), A.5-29 A.5-10 XL-MCL Manual Sample Station Components (See Table A.5-10), A.5-32 A.5-11 XL Manual Sample Station Components (See Table A.5-11), A.5-36...
Page 36 CONTENTS TABLES 2.2-1 Analyzer Backplane’ s Function, 2.2-6 3.1-1 Required Operating Clearance, 3.1-1 3.1-2 Power Requirements, 3.1-2 3.1-3 Environmental Requirements, 3.1-3 3.7-1 Oscilloscope Settings, 3.7-3 3.8-1 MCL CPU Card - S1 Settings, 3.8-2 3.11-1 Bar-Code Printer DIP Switch Settings, 3.11-2 3.12-1 Default Configuration - Bar-Code Hand-Held Scanner, 3.12-1 3.13-1 CMOS...
Page 37 CONTENTS 8.2-15 VL22 - Exploded View of Upper Valve (See Figure 8.2-15), 8.2-28 8.2-16 Bracket and Fittings - Upper Pneumatics Drawer (See Figure 8.2-16), 8.2-29 8.2-17 VL21 - Exploded View of Lower Valve (See Figure 8.2-17), 8.2-30 8.2-18 Quick Disconnects Below Upper Pneumatics Drawer (See Figure 8.2-18), 8.2-31 8.2-19 Optical Collection Area, Rear View (See Figure 8.2-19), 8.2-33...
Page 38: View
CONTENTS 8.2-52 System Pressure Adjustment Knob and Related Components (See Figure 8.2-52), 8.2-86 8.2-53 Vacuum and Air Filters with Related Components (See Figure 8.2-53), 8.2-87 8.2-54 System Vacuum Gauge and Related Components (See Figure 8.2-54), 8.2-88 8.2-55 Power Supply Module - Right Side with Cover Removed (See Figure 8.2-55), 8.2-89 8.2-56 Waste Container (See Figure...
Page 39 CONTENTS F .1-2 Code-Related Specifications, F .1-2 F .2-1 MCL Bar-Code Scanner - Default Configuration, F .2-2 F .3-1 Hand-Held Bar-Code Scanner - Default Configuration, F .3-1 F .3-2 Hand-Held Bar-Code Scanner - General Parameters, F .3-2 F .3-3 Hand-Held Bar-Code Scanner - Symbologies, F .3-2 F .3-4 Hand-Held Bar-Code Scanner - Keyboard Wedge...
Page 40 CONTENTS PN 4237029F...
Page 41: Contents
CONTENTS INTRODUCTION, 1.1-1 MANUAL DESCRIPTION, 1.1-1 Scope, 1.1-1 Notification of Updates, 1.1-1 Intended Audience, 1.1-1 Organization, 1.1-2 Numbering Format, 1.1-3 Special Headings, 1.1-3 WARNING, 1.1-4 CAUTION, 1.1-4 IMPORTANT, 1.1-4 ATTENTION, 1.1-4 Note, 1.1-4 Conventions, 1.1-4 SAFETY PRECAUTIONS, 1.2-1 Special, 1.2-1 Electronic, 1.2-1 Biological, 1.2-2 Laser, 1.2-2...
Page 42 CONTENTS 1-ii PN 4237029F...
Page 43: Introduction
Intended Audience To use this manual effectively, you need the following: Beckman Coulter authorized service training on the XL flow cytometer and the XL-MCL flow cytometer. PN 4237029F...
Page 44: Organization
Chapter 2, INSTRUMENT DESCRIPTION - An introduction to the instrument a description of: how it functions, its major components, and Beckman Coulter networking. Chapter 3, INSTALLATION PROCEDURES - PART A: A list of preinstallation requirements and all necessary instrument installation procedures. PART B: Installation procedures for all available upgrades and options.
Page 45: Numbering Format
INTRODUCTION MANUAL DESCRIPTION Chapter 7, TROUBLESHOOTING - Level-sense indicator information and an in-depth error message table. Chapter 8, PARTS LISTS - The master parts list followed by the illustrated parts. Appendix A, QUICK REFERENCE INFORMATION - Quick reference information including: tolerances and limits, circuit board layouts with applicable jumper and switch settings, protocol parameters, Prefinal Service Software tests, a list of MCL option commands, Cytometer component locations and functions, Power Supply component locations and functions, as well as a description of the AMI ATLAS PCI motherboard.
Page 46: Warning
INTRODUCTION MANUAL DESCRIPTION WARNING A WARNING indicates a situation or procedure that, if ignored, can cause serious personal injury. The word WARNING is in bold-faced text in the printed manual and is red in the electronic manual. CAUTION A CAUTION indicates a situation or procedure that, if ignored, can cause damage to the instrument.
Page 47: Safety Precautions
WARNING Risk of personal or operator injury. Covers and interlocks are installed on Beckman Coulter instruments to prevent injury from operating components. If you must remove covers or disable interlocks to service an instrument: 1.
Page 48: Biological
INTRODUCTION SAFETY PRECAUTIONS CAUTION Risk of damage to electronic components. Electrostatic discharge (ESD) can damage disk drives, add-in circuit cards, and other electronic components. If there is a possibility of ESD damage with a procedure, then do that procedure at an ESD workstation, or wear an antistatic wrist strap attached to a metal part of the chassis connected to an earth ground.
Page 49: Radiation Hazards
Risk of radiation exposure. To reduce the risk of exposure to radiation, do not use controls or adjustments or perform any procedures other than those specified in this manual. In its design and manufacture, Beckman Coulter has complied with requirements governing the use/application of lasers according to regulatory documents issued by the U.S.
Page 50 LASER RADIATION LASER RADIATION WHEN OPEN AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT AVOID EYE OR SKIN EXPOSURE TO DIRECT OR SCATTERED RADIATION CYTOMETER READY LASER SAMPLE FLOW MED HIGH Coulter Epics XL / MCL 7029085F 1.2-4 PN 4237029F...
Page 51 INTRODUCTION SAFETY PRECAUTIONS Figure 1.2-3 Laser Warning Labels - Cytometer, Optical Area, Front View CYTOMETER READY LASER SAMPLE FLOW MED HIGH Coulter Epics XL • MCL AVOID EXPOSURE VISIBLE AND / OR INVISIBLE LASER RADIATION IS EMITTED FROM THIS APERTURE. VISIBLE AND / OR AVOID EXPOSURE INVISIBLE LASER WHEN OPEN...
Page 52 INTRODUCTION SAFETY PRECAUTIONS Figure 1.2-5 Laser Certification Label - Cytometer, Back THIS LASER PRODUCT CONFORMS TO THE PROVISIONS OF 21 CFR, SUBCHAPTER J, SECTIONS 1040.10 AND 1040.11. Manufactured: DATE OF COULTER CORPORATION Hialeah Fl, 2413944 If MCL option installed, label is here also.
Page 53: Troubleshooting
Cytometer could injure or contaminate you. Beckman Coulter recommends that you wear barrier protection, such as appropriate safety glasses, a lab coat, and gloves throughout the performance of service, maintenance, and troubleshooting procedures to avoid contact with cleaners and residual fluids in the Cytometer.
Page 54 INTRODUCTION SAFETY PRECAUTIONS 1.2-8 PN 4237029F...
Page 55 CONTENTS INSTRUMENT DESCRIPTION, 2.1-1 SYSTEM OVERVIEW, 2.1-1 Function, 2.1-1 Sheath Flow, 2.1-1 Sample Flow, 2.1-1 Segmenting Valve/Positions Flow, 2.1-1 Components, 2.1-1 Cytometer (All Signal Processing Electronics), 2.1-2 Power Supply Module, 2.1-2 Computer Workstation, 2.1-3 MCL Option - Theory of Operation, 2.1-3 CYTOMETER, 2.2-1 Optics, 2.2-2 Laser, 2.2-2...
Page 56: Mesh
CONTENTS Topologies, 2.5-2 Mesh, 2.5-2 Star, 2.5-2 Linear Bus, 2.5-3 Ring, 2.5-4 Bridges, Repeaters, Routers, 2.5-4 Specifications, 2.5-5 Dedicated vs. Non-Dedicated Server, 2.5-5 LANtastic™ Network Operating System, 2.5-5 ILLUSTRATIONS 2.1-1 XL Cytometer with MCL Option, Workstation, and Power Supply Module, 2.1-1 2.1-2 Power Supplies in Power Supply Module, 2.1-2...
Page 57: Instrument Description
INSTRUMENT DESCRIPTION SYSTEM OVERVIEW Function The instrument is a flow cytometer analyzer that reports three- and four-color surface marker analysis and one-color DNA analysis. Sheath Flow Set to 4.0 psi (controlled by mechanical regulator). Sample Flow User selectable to the following approximations when calibrated (controlled electronically): LOW = 3.72 psi MED = 3.92 psi HIGH = 4.12 psi.
Page 58: Cytometer (All Signal Processing Electronics)
INSTRUMENT DESCRIPTION SYSTEM OVERVIEW Cytometer (All Signal Processing Electronics) The instrument’ s Cytometer contains the: Fluids and pneumatic components Laser, flow cell, and optics to analyze cells. Additionally, the XL-MCL flow cytometers contain the MCL option (Figure 2.1-1). Power Supply Module The instrument’...
Page 59: Computer Workstation
INSTRUMENT DESCRIPTION SYSTEM OVERVIEW Computer Workstation The Computer Workstation is composed of (Figure 2.1-3): An INTEL Pentium processor (including the Opto Transprocessor EXMEM II card), A 3.5 in. floppy diskette drive, A CD-ROM drive, A mouse (including a mouse pad), A keyboard (small or large), and A color monitor.
Page 60 INSTRUMENT DESCRIPTION SYSTEM OVERVIEW ® ® Upon power up, the COULTER EPICS XL/XL-MCL SYSTEM Software VER 2.1 (hereafter referred to as SYSTEM II software) detects the presence of the MCL Interface card to enable the operational code needed to support the XL-MCL flow cytometer. Commands are then sent out through the parallel interface cable to the MCL Controller card that is mounted to the XL-MCL flow cytometer’...
Page 61 INSTRUMENT DESCRIPTION SYSTEM OVERVIEW If no label is read, a NO READ appears on the histogram report alerting the user that no label was present or that it was not read correctly. Next the test tube lifter solenoid (LV) fires to raise the test tube.
Page 62 INSTRUMENT DESCRIPTION SYSTEM OVERVIEW 2.1-6 PN 4237029F...
Page 63: Cytometer
INSTRUMENT DESCRIPTION CYTOMETER CYTOMETER The Cytometer contains all the necessary optics, fluidics, and electronics required to analyze samples; the laser head, optical housing assembly, flow cell, sheath container, and cleaning agent container. All the pinch valves, fixed regulators, plumbing, ductwork, and the Data Acquisition card cage are housed in the Cytometer to control sample input, sense, amplify, discriminate, and digitize signals.
Page 64: Optics
INSTRUMENT DESCRIPTION CYTOMETER Optics The instrument’ s optics system requires no customer alignment. The system produces DNA quality Coefficient of Variation (CV) and detects <1000 molecules of fluorescein. Minimal alignment is performed by Field Engineers using a special alignment tool. Access is gained by lifting the front panel and securing it in place.
Page 65: Pinhole Assembly
INSTRUMENT DESCRIPTION CYTOMETER The flow cell (Figure 2.2-3) sensing area consists of a 250-µ square quartz channel with an integral lens mounted with a vertical (upward) flow path. A stream of sheath fluid, pressurized at a constant 4 psi, enters the channel at the lower end and flows upward (Figure 2.2-3).
Page 66: Forward Scatter (Fs) Detector
INSTRUMENT DESCRIPTION CYTOMETER Forward Scatter (FS) Detector The forward scatter detector (also referred to as the FS detector or FS sensor) is a solid-state diode and is equipped with a slide-into-place ND 1 filter. Laser Intersection The Cytometer uses an air-cooled 488 nm Argon laser (set to 20 mW) to excite the sample in the core stream in the flow cell area.
Page 67 INSTRUMENT DESCRIPTION CYTOMETER Located in the Cytometer's upper half, the Analyzer backplane uses a modified version of the VME standard. The card connectors are two-keyed, 96-pin, DIN connectors (to ensure that the cards are not installed backwards) and provide an airtight seal for improved reliability. All connectors on the Analyzer backplane are keyed and are of a different size.
Page 68: Amplifier (Amp)/Signal Conditioner Card
INSTRUMENT DESCRIPTION CYTOMETER The Analyzer backplane is a multi-layered card with each layer (plane) having a specific function as shown in Table 2.2-1. Table 2.2-1 Analyzer Backplane’s Function Plane Function 1 (bottom) Chassis GND, 24 V GND, 24 Vdc / solenoid signals Signal (VME, low level dc and control) / +5 V copper Digital GND, -15 Vdc Signals (digital, analog, custom bus, digital GND)
Page 69 INSTRUMENT DESCRIPTION CYTOMETER Figure 2.2-6 Amplifier Analog Bus Interface Data Bus 2 Diode sensor optical gain 90° LS Data Bus 1/TRIGGER Diode sensor ADC Bus AUX Bus PMT 4 PMT 3 PMT 2 PMT 1 Option amplified for Stretched Pulse Amplified Pulse for System Trigger Analog Bus functions are software selectable Stretched Pulse for ADC conversion...
Page 70: Trans Data Acquisition Card
INSTRUMENT DESCRIPTION CYTOMETER Trans Data Acquisition Card The Trans Data Acquisition card provides event detection through a single-trigger discriminator driven by any one of the Amp/Signal Conditioner cards. The trigger level is set by a DAC. When an event is detected, a state machine on the Trans Data Acquisition card sequences the peak sense and hold circuits on the Amp/Signal Conditioner cards.
Page 71: System Interface Card
INSTRUMENT DESCRIPTION CYTOMETER System Interface Card The System Interface card provides the system interface required to control all pneumatics, fluidics, and laser systems using both digital and analog signal control (Figure 2.2-8). Figure 2.2-8 System Interface Card Block Diagram FAN AND LASER BLOWER CONTROL PNEUMATIC CONTROL...
Page 72: Cyto Transputer Card
INSTRUMENT DESCRIPTION CYTOMETER Cyto Transputer Card The Cytometer (Cyto) Transputer card provides the instrument with a dedicated control and data processing system composed of two programmed 400 series INMOS transputers. The card receives signals from the Cytometer push-button switches and from the PC Transprocessor.
Page 73: Mcl Interface Card
INSTRUMENT DESCRIPTION CYTOMETER MCL Interface Card Note: The MCL Interface card is present only when the MCL option is installed. In an XL-MCL flow cytometer, the MCL Interface card provides parallel interface to the MCL CPU card (Figure 2.2-10). The MCL Interface card is located in the Data Acquisition card cage and the MCL CPU card is mounted to the MCL frame.
Page 74: Optical Transprocessor Extended Memory Cards
INSTRUMENT DESCRIPTION CYTOMETER Optical Transprocessor Extended Memory Cards The Optical (Opto) Transprocessor Extended Memory (EXMEM or EXEM) card (located in the Workstation tower computer) provides the communication interface from the Cytometer to the Computer Workstation. Two versions of this circuit card exist - the original circuit card referred to as the Opto Transprocessor EXMEM card and the new circuit card referred to as the Opto Transprocessor EXMEM II card.
Page 75: Fiber Optic Interface Card
INSTRUMENT DESCRIPTION CYTOMETER Structurally, a transputer is a monolithic device containing an integer processor, fast memory, and multiple serial communications links allowing point-to-point connections with additional transputers. Communication between links runs simultaneously to maximize the performance of processor computation. Information passes bidirectionally on two wires between one pair of transputers in a computing network.
Page 76 INSTRUMENT DESCRIPTION CYTOMETER 2.2-14 PN 4237029F...
Page 77: Power Supply Module
INSTRUMENT DESCRIPTION POWER SUPPLY MODULE POWER SUPPLY MODULE Description The Power Supply module supplies pressure, vacuum, laser power, and all dc voltages required to the Cytometer (Figures 2.3-1 Figure 2.3-2). The power provided by the Power Supply module is distributed via the interface harness. The Power Supply module can be installed under the Cytometer or next to it on a bench.
Page 78 INSTRUMENT DESCRIPTION POWER SUPPLY MODULE Figure 2.3-2 Power Supply Module - Power Module Control Card Flow Diagram POWER LASER REMOTE CONTROL MODULE TO LASER CONTROL POWER CONTROL INPUT POWER CABLE SUPPLY FROM CYTOMETER LASER INTERLOCK CONTROL LINE AC TO LASER LASER POWER POWER SUPPLY...
Page 79 INSTRUMENT DESCRIPTION POWER SUPPLY MODULE Figure 2.3-4 Power Supply Module - Rear CIRCUIT BREAKERS POWER CABLES: LOGIC, ANALOG,AND MCL OPTION LASER UMBILICAL CABLE POWER MODULE CONTROL CARD WASTE LEVEL AUX POWER ON TUBING CONNECTOR SYSTEM PRESSURE QUICK ON/OFF WASTE LEVEL DISCONNECTS VACUUM POWER ON/OFF...
Page 80: Linear Power Supply
INSTRUMENT DESCRIPTION POWER SUPPLY MODULE Linear Power Supply The Linear Power supply consists of five separate power supplies (Figure 2.3-6): XL flow cytometers - +5 Vdc ± 15 Vdc +24 Vdc XL-MCL flow cytometers - +5 and ± 12 Vdc +24 Vdc.
Page 81: Workstation
WORKSTATION The Workstation analyzes and reports the status of the Cytometer. The Workstation computer contains a Beckman Coulter-manufactured Opto Transprocessor EXMEM card or Opto Transprocessor EXMEM II card. The Fiber Optics Interface card attached to the rear panel of the Cytometer converts the digital data received from the Cyto Transputer card to optical data...
Page 82: Options
INSTRUMENT DESCRIPTION WORKSTATION Options Options currently available: MCL option Media storage: Iomega™ ZIP™ drive CD-RW drive 4-Color PMT Multimedia Workstation Serial/Parallel Adapter Interface card Artisoft LANtastic Client/Server Software V8.0 ® ™ Sybase SQL Anywhere PC database server software EXPO32™ ADC software EXPO32™...
Page 83: Networking
Examples of bounded media are: microwave, infrared, and laser links. In order to connect the media, the Network Interface card (NIC) that is chosen must support the medium type. The Beckman Coulter network supports T.P ., coaxial (thin), and AUI (thick).
Page 84: Topologies
INSTRUMENT DESCRIPTION NETWORKING Topologies To connect the media, a layout of the connections must be planned. The layout is referred to as the physical network topology. There are four commonly used topologies: Mesh Star Linear Bus, and Ring. Mesh A mesh topology has point-to-point connections (Figure 2.5-1).
Page 85: Linear Bus
INSTRUMENT DESCRIPTION NETWORKING Figure 2.5-2 Star Topology DEVICE 7029058D Linear Bus In a bus topology, or linear bus, all the nodes on a network attach directly and the bus terminates at both ends (Figure 2.5-3). Data flows in both directions from the sending computer.
Page 86: Ring
INSTRUMENT DESCRIPTION NETWORKING Ring A ring topology is as the name implies, a closed loop, point-to-point connection (Figure 2.5-4). Repeaters are used between network nodes to duplicate the signal and minimize signal loss. Fiber optics use the ring topology as well as IBM’ s Token Ring. Figure 2.5-4 Ring Topology 7029060D Bridges, Repeaters, Routers...
Page 87: Specifications
The Beckman Coulter network, when assembled, provides the user with a complete network to transfer data for archival purposes. Beckman Coulter recommends that the server be set up as a dedicated file server.
Page 88 INSTRUMENT DESCRIPTION NETWORKING 2.5-6 PN 4237029F...
Page 89: Front
CONTENTS INSTALLATION PROCEDURES, 3.1-1 PART A: INSTRUMENT INSTALLATION PRE-SITE INSPECTION, 3.1-1 Space and Accessibility, 3.1-1 Power Requirements, 3.1-2 Optional Computer Peripherals, 3.1-3 Environment, 3.1-3 Unloading and Moving the Instrument, 3.1-3 Installation Assistance, 3.1-4 Inspection Report, 3.1-4 PREINSTALLATION CHECKS, 3.2-1 Pre-Site Inspection Compliance, 3.2-1 Education Center Training, 3.2-1...
Page 90 CONTENTS Monitor Connections: 22-inch CRT Monitor, 3.4-3 Connecting the Monitor Cables, 3.4-3 Printer Option, 3.4-3 CABLE/TUBING CONNECTIONS, 3.5-1 Connect Cables, 3.5-1 Laser Umbilical Cable, 3.5-1 Logic Cable, 3.5-3 Analog Cable, 3.5-3 Power Module Control Cable, 3.5-3 Make Appropriate MCL Connections, 3.5-3 Fiber Optic Interface Cables, 3.5-4 CYT12...
Page 91 CONTENTS Alignment, 3.8-3 Operational Verification, 3.8-3 Reinstall Covers, 3.8-3 System Verification, 3.8-4 4-COLOR PMT UPGRADE, 3.9-1 Purpose, 3.9-1 Tools/Supplies Needed, 3.9-1 Preparation, 3.9-1 Hardware Installation, 3.9-1 Amp/Signal Conditioner Card Calibration, 3.9-3 PMT Operational Alignment, 3.9-3 Reinstall Covers, 3.9-3 System Verification, 3.9-3 ™...
Page 92 CONTENTS 3.13 SERIAL/PARALLEL ADAPTER INTERFACE CARD OPTION, 3.13-1 Purpose, 3.13-1 Tools/Supplies Needed, 3.13-1 Removal, 3.13-1 Installation, 3.13-1 Verification, 3.13-2 3.14 NETWORK SETUP USING MICROSOFT WINDOWS 95 OR 98, 3.14-1 Purpose, 3.14-1 Tools/Supplies Needed, 3.14-1 Software Installation, 3.14-1 Cable Connections, 3.14-2 Set Up Hard Drives and Printers, 3.14-2 Define the Computer Name and...
Page 93: Assembly
CONTENTS 3.19 EXPO32™ SOFTWARE OPTIONS, 3.19-1 Purpose, 3.19-1 Tools/Supplies Needed, 3.19-1 Preparation, 3.19-1 For EXPO32 ADC Installation Option, 3.19-1 For Printer Installation, 3.19-1 Software Installation, 3.19-1 Verification, 3.19-1 ILLUSTRATIONS 3.3-1 Location of Foam Shipping Blocks Used to Protect the Compressor Assembly, 3.3-3 3.3-2 Location of Small Shipping Screws Used to Secure the Side...
Page 94 CONTENTS TABLES 3.1-1 Required Operating Clearance, 3.1-1 3.1-2 Power Requirements, 3.1-2 3.1-3 Environmental Requirements, 3.1-3 3.7-1 Oscilloscope Settings, 3.7-3 3.8-1 MCL CPU Card - S1 Settings, 3.8-2 3.11-1 Bar-Code Printer DIP Switch Settings, 3.11-2 3.12-1 Default Configuration - Bar-Code Hand-Held Scanner, 3.12-1 3.13-1 CMOS Settings, 3.13-2...
Page 95: Installation Procedures
INSTALLATION PROCEDURES PART A: INSTRUMENT INSTALLATION PRE-SITE INSPECTION Prior to installing an XL or XL-MCL flow cytometer, a pre-site inspection is required to verify the following conditions: Space and Accessibility Power Requirements Environment Unloading and Moving the Instrument Installation Assistance Inspection Report Space and Accessibility A bench or table must be available for installation.
Page 96: Power Requirements
INSTALLATION PROCEDURES PRE-SITE INSPECTION Power Requirements The XL or XL-MCL Cytometer and Power Supply module are contained in two separate enclosures with two separate power cables - one for system power and the other for the laser. Each power cord is intended to be plugged into a different 20 ampere supply from the other. The electrical ratings of the XL or XL-MCL system are: 100 Vac system: 48 to 62 Hz, 16.00 amps, 1600 watts 120 Vac system: 48 to 62 Hz, 8.00 amps, 1840 watts...
Page 97: Optional Computer Peripherals
Ambient temperature 18° - 29°C (64° - 85°F) Stability/rate of change Temperature fluctuations within the ambient temperature range can affect performance. For optimal performance, Beckman Coulter recommends the room temperature not fluctuate more than 2.8°C (5°F) per hour. Noise 65 dba...
Page 98: Installation Assistance
INSTALLATION PROCEDURES PRE-SITE INSPECTION Installation Assistance Inform the customer that during the installation you will need an able-bodied assistant to help you lift and position the Cytometer on the bench or table top. Ask them to make sure someone is available to help as needed. Inspection Report Review the findings with your contact person.
Page 99: Preinstallation Checks
PREINSTALLATION CHECKS PREINSTALLATION CHECKS PREINSTALLATION CHECKS Before installing the instrument, verify the following conditions: Pre-Site Inspection Compliance Education Center Training Condition of Cartons Received Optional Computer Peripherals Supplies Pre-Site Inspection Compliance Prior to installing the XL or XL-MCL flow cytometer, verify the site complies with the space, accessibility, power, and environmental requirements detailed in the pre-site inspection.
Page 100 PREINSTALLATION CHECKS PREINSTALLATION CHECKS 3.2-2 PN 4237029F...
Page 101: Initial Installation
WARNING Risk of personal injury. Installation of an XL flow cytometer by anyone who has not successfully completed a Beckman Coulter authorized training course is prohibited. Avoid personal injury by obtaining the necessary training to complete these installation procedures. Tools/Supplies Needed...
Page 102: Power Supply Installation
INSTALLATION PROCEDURES INITIAL INSTALLATION Inspect the Cytometer for any physical damage caused in shipping. Report any damage on the Lotus Notes database, WW: Cytometry Install. Correct as needed. WARNING Risk of personal injury. Do not attempt to unload the Cytometer without assistance. The Cytometer is heavy and can be difficult to lift and maneuver into position.
Page 103: Cytometer Installation
INSTALLATION PROCEDURES INITIAL INSTALLATION Figure 3.3-1 Location of Foam Shipping Blocks Used to Protect the Compressor Assembly Inspect for any loose or damaged parts. Report any loose or damaged parts/assemblies on the on the Lotus Notes database, WW: Cytometry Install. Correct as needed.
Page 104 INSTALLATION PROCEDURES INITIAL INSTALLATION Figure 3.3-2 Location of Small Shipping Screws Used to Secure the Side Covers Remove shipping screws Left side Right side 7029103F Remove the right-side cover. Manually pull the right-side cover up and off the instrument. Set the cover aside. Use instrument specific instructions to gain required access: If this is an XL cytometer without the MCL option, go to step 5.
Page 105 INSTALLATION PROCEDURES INITIAL INSTALLATION Remove the two screws securing the MCL probe housing (Figure 3.3-3) then carefully pull the probe housing cover off the Cytometer and set it aside. Figure 3.3-3 Removing the MCL Probe Housing Cover Remove screws 7029238F Close the MCL door (upper cover) and unlatch the MCL lower cover by grasping the base and gently pulling the base away from the Cytometer (Figure...
Page 106 INSTALLATION PROCEDURES INITIAL INSTALLATION 11. Remove the center front cover (filter cover) and set it aside. See Figure 3.3-5. Figure 3.3-5 Remove the Center Front Cover (Filter Cover) 7029230F 12. Pull open the reagent drawer. 13. Remove the manual sample station cover assembly: Carefully move the unlatched MCL cover away from the Cytometer frame.
Page 107 INSTALLATION PROCEDURES INITIAL INSTALLATION 14. Manually pull the left-side cover up and off the Cytometer frame. Set the cover aside. CAUTION Risk of damage to electrical components on the front panel display circuit card. Do not use a T-handled hex key to hold the upper cover open. If the metal in the T-handle comes in contact with the electrical components on the circuit card, the components may short out and the card becomes defective.
Page 108: Remove The Optical Bench Shipping Tie-Down In The Front
INSTALLATION PROCEDURES INITIAL INSTALLATION Figure 3.3-8 Filter Shield Screw Locations Remove screws (4) Filter shield 7029116F Remove the Optical Bench Shipping Tie-Down in the Front Remove the optical bench shipping tie-down (screw and hex nut) located at the front right corner of the optical bench: Slide the reagent drawer open as needed.
Page 109: Remove The Optical Bench Shipping Tie-Down In The Back (Behind The Laser)
INSTALLATION PROCEDURES INITIAL INSTALLATION Figure 3.3-9 Remove the Optical Bench Shipping Tie-Down in the Front Chapman ratchet 3/8 inch Phillis-head bit Spacer 3/8 inch ratchet 7029107F Remove the spacer (Figure 3.3-9). Place the shipping tie-down (screw, hex nut, and spacer) in the box designated for storing shipping parts.
Page 110 INSTALLATION PROCEDURES INITIAL INSTALLATION Figure 3.3-10 MCL CPU Card Cable Locations Figure 3.3-11 MCL CPU Card Screw Locations MCL CPU card MCL CPU card Disconnect Remove cable screws 7029100F 7029093F Set up your static-dissipative field kit (ESD kit). Disconnect the five cable connectors attached to the card (Figure 3.3-10).
Page 111 INSTALLATION PROCEDURES INITIAL INSTALLATION Before beginning this task, make sure you have a long Phillips-head screwdriver ATTENTION: (or a Phillips-head screwdriver with an extension), a 3/8-inch wrench, and a piece of electrical tape. If either the hex nut or spacer falls to the Cytometer floor, it must be retrieved. Use a 3/8-inch wrench and a long Phillips-head screwdriver to remove the shipping tie-down (screw and hex nut).
Page 112: Prepare Components Inside The Lower Pneumatics Drawer For Operation
INSTALLATION PROCEDURES INITIAL INSTALLATION Prepare Components inside the Lower Pneumatics Drawer for Operation On the left side of the Cytometer, locate the two rows of pinch-valves inside the lower pneumatics drawer. Use a hemostat to remove the red shipping clip from each pinch valve. Make sure you remove all 14 shipping clips! Count the removed shipping clips to confirm that all 14 red clips were removed.
Page 113: Prepare The Segmenting Valve For Operation
INSTALLATION PROCEDURES INITIAL INSTALLATION Remove the shipping brace (Figure 3.3-14). Place the brace and screws in the box designated for storing shipping parts. Inspect for any lose cards or cables. Correct as needed. Disassemble the static-dissipative field kit. Prepare the Segmenting Valve for Operation Locate the segmenting valve (behind the manual sample station removed earlier).
Page 114: Prepare Components Inside The Upper Pneumatic Drawer For Operation
INSTALLATION PROCEDURES INITIAL INSTALLATION Disconnect the air cylinder from the middle segmenting pad and carefully slide the pad off the shaft. Disconnect the air cylinder from the rear segmenting pad and carefully slide the pad off the shaft. Rinse each segmenting pad with distilled water then reinstall the pad on the shaft: Rinse the rear segmenting pad with distilled water and reinstall the pad on the shaft.
Page 115: Fill The Reagent Containers
INSTALLATION PROCEDURES INITIAL INSTALLATION Fill the Reagent Containers IMPORTANT Risk of misleading results if you contaminate either reagent. Be careful not to contaminate the sheath fluid or cleaning reagent. Do not let your fingers, paper towels, or other objects touch the inside of the container or the inside of the cap.
Page 116: Fill The Cleaning Agent Container
INSTALLATION PROCEDURES INITIAL INSTALLATION Fill the Cleaning Agent Container Unscrew the cap on the cleaning agent container (smaller container on your left). Lay the cap upside down on the container to avoid contamination. Insert a funnel to help avoid spills. Carefully pour two 500 mL bottles of cleaning agent into the container.
Page 117: Workstation Installation
INSTALLATION PROCEDURES WORKSTATION INSTALLATION WORKSTATION INSTALLATION “Workstation” or “Computer Workstation” is commonly used to refer to the FlowCentre Multimedia Workstation. This system consists of a tower computer, monitor, keyboard, mouse, and any optional equipment such as a Printer. The tower computer, monitor, and Printer each have their own ac power cord and power on/off switch.
Page 118: Mouse Connection
INSTALLATION PROCEDURES WORKSTATION INSTALLATION Mouse Connection Connect the mouse cable to the mouse port on the rear of the tower computer (Figure 3.4-2). Figure 3.4-2 Electronic Cable Connections - Mouse and Keyboard Mu litSync LCD1700 M+ C A UTIO N AV IS AC HTUN G AD VARS EL...
Page 119: Monitor Connections: 22-Inch Crt Monitor
INSTALLATION PROCEDURES WORKSTATION INSTALLATION Attach the monitor plug to the appropriate jack on the rear of the tower computer (Figure 3.4-3). If this is the only monitor, attach the plug to the right jack on the Dual Monitor Video card. If this is the second monitor, attach the plug to the left jack on the Dual Monitor Video card.
Page 120 INSTALLATION PROCEDURES WORKSTATION INSTALLATION 3.4-4 PN 4237029F...
Page 121: Cable/Tubing Connections
INSTALLATION PROCEDURES CABLE/TUBING CONNECTIONS CABLE/TUBING CONNECTIONS Connect Cables ATTENTION: Refer to Figure 3.5-1 as needed. Figure 3.5-1 Instrument Connections Cytometer Workstation FIBER OPTIC REC FIBER OPTIC TRANS Mu litSync LCD1700 M+ C A UTIO N AV IS AC HTUN G AD VARS EL WA RNI NG MO DE L...
Page 122 INSTALLATION PROCEDURES CABLE/TUBING CONNECTIONS Route the two large connectors on the end of the laser umbilical cable through the opening in the rear of the Power Supply (Figure 3.5-1). Note: Remove the ac power plug for easier access to the sockets. Connect the laser umbilical cable to the Laser Power Supply inside the Power Supply module.
Page 123: Logic Cable
INSTALLATION PROCEDURES CABLE/TUBING CONNECTIONS Use the hex nut to secure the ground wire from the laser umbilical cable to one of the screws securing the small cover to the rear cover. Reconnect the ac power plug. Reinstall the laser power supply key then rotate the key clockwise to the — position. (The power supply will not be activated until ac is applied later.) Logic Cable Locate the logic cable.
Page 124: Fiber Optic Interface Cables
INSTALLATION PROCEDURES CABLE/TUBING CONNECTIONS Fiber Optic Interface Cables Locate the orange fiber optic interface cable. This cable is actually two cables joined together so it has two terminal posts (with a locking nut) at each end. One cable is labeled RX and the other is labeled TX. The terminal posts on one end of the cable will be inserted into jacks on the Cytometer and the terminal posts on the other end of the cable will be inserted into jacks on the Opto Transprocessor EXMEM II card in the tower computer.
Page 125: Cyt12 Cable
INSTALLATION PROCEDURES CABLE/TUBING CONNECTIONS CYT12 Cable Locate the orange fiber optic interface cable with a single terminal post (with a locking nut) at each end. Remove the red plastic cap protecting each terminal post. Place these caps with the other parts used for shipping.
Page 126: Tubing Connections
INSTALLATION PROCEDURES CABLE/TUBING CONNECTIONS Tubing Connections Locate the bundle of shielded pneumatic/hydraulic tubings (Figure 3.5-4). Figure 3.5-4 Bundle of Shielded Pneumatic/Hydraulic Tubings For waste container Connect to Connect to Power Supply Cytometer rear panel module rear panel 7029099F There are three groups of tubings: The float liquid level sensor and cap are for the waste container.
Page 127 INSTALLATION PROCEDURES CABLE/TUBING CONNECTIONS Figure 3.5-5 Tubing Connections Cytometer TRANS FLOW CELL REC. WASTE PRESSURE VACUUM WASTE WASTE PRESSURE VACUUM Power Supply module AUX POWER ON SYSTEM PRESSURE ON/OFF WASTE LEVEL VACUUM WASTE WASTE LASER POWER SYSTEM POWER MAX 1800 WATTS MAX 1500 WATTS WASTE VENT WASTE VENT...
Page 128 INSTALLATION PROCEDURES CABLE/TUBING CONNECTIONS Because there are two orange connectors in this tubing bundle, you must use the ATTENTION: tubing labels to ensure proper connection. Connect the orange female quick-connect on the tubing labeled WASTE to the orange male quick-connect labeled on the rear of the Cytometer.
Page 129: Software Installation
SOFTWARE INSTALLATION SOFTWARE INSTALLATION Tools/Supplies Needed B COULTER EPICS XL / XL-MCL SYSTEM II software, Version 3.0, PN 6706441 SYSTEM II Software Installation Ensure the Workstation and two Power Supply module ac power line cords are connected to appropriate wall outlets.
Page 130 INSTALLATION PROCEDURES SOFTWARE INSTALLATION 3.6-2 PN 4237029F...
Page 131: Operational Checks
INSTALLATION PROCEDURES OPERATIONAL CHECKS OPERATIONAL CHECKS Tools/Supplies Needed B One, empty, 12 x 75-mm test tube, PN 2523749 B Flow-Check fluorospheres, PN 6605359 B Oscilloscope B Digital Voltmeter (DVM) Preliminary Operational Checks Verify all cooling fans are operating. Refer to Heading A.5 for component locations in the Cytometer and refer to...
Page 132 INSTALLATION PROCEDURES OPERATIONAL CHECKS Press and hold the purge (vent) button (Figure 3.7-1) until the bubbles in the vent tubing are gone (Figure 3.7-2). Figure 3.7-1 Purge (Vent) Button Location Purge (vent) button 7029117F Figure 3.7-2 Purging Bubbles from the Sheath Filter Press the PRIME button three more times.
Page 133: Initial System Verification
INSTALLATION PROCEDURES OPERATIONAL CHECKS Initial System Verification Power Supply Verification Go to Heading 4.20, POWER SUPPLY VOLTAGE VERIFICATION AND ADJUSTMENT verify all power supply voltages (including MCL) and make needed adjustments. Trans Data Acquisition Card Offset Verification Go to Heading 4.14, TRANS DATA ACQUISITION CARD REPLACEMENT AND/OR CALIBRATION and complete the procedure as written.
Page 134: Reinstall Covers And Panels
INSTALLATION PROCEDURES OPERATIONAL CHECKS 10. Use instrument specific instructions to proceed: If this is an XL cytometer without the MCL option, go to Heading 4.6, OPTICAL ALIGNMENT and complete the procedure as written to obtain the best CVs. If this is an XL cytometer with the MCL option, go to step to verify the MCL is functioning properly.
Page 135: Right-Side Cover
INSTALLATION PROCEDURES OPERATIONAL CHECKS Loosely install the two flat-head, Phillips screws on the right. Tighten all four screws to secure the cover assembly to the Cytometer frame. Close the reagent drawer. Remove the tool bracing the upper cover open and gently lower the cover. Reinstall the center front cover (covering the filters).
Page 136: Clean Up
INSTALLATION PROCEDURES OPERATIONAL CHECKS Clean Up CAUTION Risk of damage to the instrument. The instrument will be damaged if it is operated without removing all 16 red shipping clips (14 in the lower pneumatic drawer and 2 in the upper pneumatic drawer) and the two plastic shipping insert from the segmenting valve.
Page 137: Part B: Upgrades And Options Installation
INSTALLATION PROCEDURES MCL OPTION UPGRADE PART B: UPGRADES AND OPTIONS INSTALLATION MCL OPTION UPGRADE Purpose Use these procedures to install an MCL option. After completing these procedures, the XL-MCL flow cytometer’ s model code must be changed from 2714 to 2988. Tools/Supplies Needed B MCL Upgrade kit in black, PN 6912669 B MCL Upgrade kit in grey, PN 6915506...
Page 138: Power Supply Module Upgrade
INSTALLATION PROCEDURES MCL OPTION UPGRADE Carefully dress the new tubing harness to the existing tubing so that it does not get pinched when the lower pneumatics drawer is reinstalled. Unpack the MCL main frame assembly and inspect it for damaged or missing parts. Set S1 on the MCL CPU card as shown on Table 3.8-1.
Page 139: Power Supply Voltage Verification And Adjustment
INSTALLATION PROCEDURES MCL OPTION UPGRADE Install the MCL power supply and secure it with four screws. Connect the ac interconnect cable (internal in the Power Supply module) to the MCL power supply. Remove the MCL loop plug from the rear of the Power Supply module and connect the MCL external power cable.
Page 140: System Verification
INSTALLATION PROCEDURES MCL OPTION UPGRADE Reinstall the Cytometer top cover. Refer to Heading 4.3 if you need detailed instructions. System Verification Perform an SVP as directed under Heading 5.1, SYSTEM VERIFICATION PROCEDURE (SVP). With the permanent marker, write the XL-MCL flow cytometer's serial number in the blank box on the new serial name plate and install the name plate on the back of the Cytometer.
Page 141: 4-Color Pmt Upgrade
INSTALLATION PROCEDURES 4-COLOR PMT UPGRADE 4-COLOR PMT UPGRADE Purpose Use this procedure to upgrade an instrument from three colors to four colors. Tools/Supplies Needed B 4-Color PMT Upgrade kit, PN 6912932 B Flow-Check fluorospheres, PN 6605359 B One, empty, 12 x 75-mL test tube, PN 2523749 Preparation Check the contents of the 4-Color PMT Upgrade kit against the packing list to ensure that all of the parts are present.
Page 142 INSTALLATION PROCEDURES 4-COLOR PMT UPGRADE Figure 3.9-1 PMT Filter Locations Band Pass Band Pass Band Pass Band Pass Dichroic Dichroic Dichroic Laser Blocker Dichroic 7029217F Remove the blank cover from the optical-housing block between PMT 2 and PMT 3. Remove the tape that is covering the PMT 3 filter slot. Install the new PMT holder between PMT 2 and PMT 3 and secure it with screws.
Page 143: Amp/Signal Conditioner Card Calibration
INSTALLATION PROCEDURES 4-COLOR PMT UPGRADE 12. Insert the filters into their correct position (Figure 3.9-1). 13. Install the filter lock-down clips and secure the filter assembly to the optical-housing assembly using screws. 14. Attach the new four-color front cover to the optical-housing assembly using screws. 15.
Page 144 INSTALLATION PROCEDURES 4-COLOR PMT UPGRADE 3.9-4 PN 4237029F...
Page 145: Flowcentre ™ Ii Multimedia Workstation Upgrade
Note: PS/2 scroll point mouse pointing device, PN 2016876, and a Windows 98 compatible keyboard, PN 2016881, are included with this tower computer. B Beckman Coulter Service Tool Kit, PN 5415102 Preparation Unpack the FlowCentre II tower computer, keyboard, and mouse.
Page 146: Keyboard Connection
INSTALLATION PROCEDURES ™ FlowCentre II MULTIMEDIA WORKSTATION UPGRADE Figure 3.10-1 Electronic Cable Connections - Mouse and Keyboard Mu litSync LCD1700 M+ C A UTIO N AV IS AC HTUN G AD VARS EL WA RNI NG MO DE L LC D170 0M + SE RI AL NO.
Page 147: Cable Connections
INSTALLATION PROCEDURES ™ FlowCentre II MULTIMEDIA WORKSTATION UPGRADE Cable Connections Fiber Optic Interface Cables ATTENTION: The orange fiber optic interface cable is actually two cables joined together so it has two terminal posts (with a locking nut) at each end. One terminal post is labeled the other terminal post is labeled The terminal posts on one end of the cable are already inserted into jacks on the Cytometer and the terminal posts (labeled...
Page 148: Cyt12 Cable
INSTALLATION PROCEDURES ™ FlowCentre II MULTIMEDIA WORKSTATION UPGRADE Figure 3.10-3 Opto Transprocessor EXMEM and EXMEM II Card Edge Locations Opto Transprocessor EXMEM ll card edge Opto Transprocessor EXMEM card edge CYT12 7029225F Insert the terminal post labeled into the inner (middle) jack labeled (Figure 3.10-3) and tighten the locking nut.
Page 149: Expo32™ Software Option Installation
INSTALLATION PROCEDURES ™ FlowCentre II MULTIMEDIA WORKSTATION UPGRADE Reboot the computer system by simultaneously pressing the Ý, Þ, and á keys. When the Microsoft Windows 98 Startup Menu appears, select 8. Previous version of MS-DOS which should select SYSTEM II software as the operating system. Verify the computer boots to the XL SYSTEM II software.
Page 150 INSTALLATION PROCEDURES ™ FlowCentre II MULTIMEDIA WORKSTATION UPGRADE 3.10-6 PN 4237029F...
Page 151: 3.11 Bar-Code Printer Option
Printer to the CPU. If you encounter any problems, please refer to the OEM-supplied manual for additional help. This options allows the XL-MCL flow cytometer to print CODE 128 Bar-code labels through the System II software. Beckman Coulter has chosen Zebra Technologies Corporation  STRIPE S-500 Bar Code Printer for the XL-MCL flow cytometer.
Page 152 INSTALLATION PROCEDURES BAR-CODE PRINTER OPTION Figure 3.11-1 Bar-Code Printer - DIP Switches SERIAL INTERFACE CABLE POWER ON/OFF SWITCH AC POWER LINE CORD 7029051D Table 3.11-1 Bar-Code Printer DIP Switch Settings Switch Setting Position Function Right Not used Right Sets BAUD rate to 9600 Right Not used Left...
Page 153: Media Calibration
INSTALLATION PROCEDURES BAR-CODE PRINTER OPTION Figure 3.11-2 Bar-Code Printer - Media Threading and Adjustment Components RIBBON RIBBON SUPPLY SUPPLY SPOOL RIBBON RELEASE BUTTON HEAD OPEN MEDIA LEVER SUPPLY OPEN PRINT HEAD ASSEMBLY MEDIA SUPPLY LABELS GUIDE PLATEN PLATEN MEDIA GUIDE ROD DRAG ROLLER BACKING...
Page 154: Xl-Mcl Flow Cytometer Connection
The label tear-off position adjustment procedure has to be performed only at installation as long as the customer uses bar-code labels and ribbons purchased from Beckman Coulter. If the customer purchases bar-code labels and ribbons from someone other than Beckman Coulter, the following procedure must be performed.
Page 155: Printer Thermal Head Adjustment
Note: Adjustment settings are automatically saved in the Printer when the power is turned off (O). Ribbon Replacement Beckman Coulter recommends that the customer order the same type of label roll (media supply) previously used. The bar-code label media replacement kit contains labels and enough ribbon to print the labels.
Page 156 INSTALLATION PROCEDURES BAR-CODE PRINTER OPTION Push in the ribbon release button (Figure 3.11-2) allowing the bars on the used ribbon holder to be unlocked. Remove the used ribbon from the ribbon supply spool (Figure 3.11-2) and discard the used ribbon. With a cotton swab and alcohol, clean the printer head (Figure 3.11-2).
Page 157: 3.12 Bar-Code Hand-Held Scanner Option
INSTALLATION PROCEDURES BAR-CODE HAND-HELD SCANNER OPTION 3.12 BAR-CODE HAND-HELD SCANNER OPTION Purpose The purpose of this procedure is to guide you in successfully installing and connecting the bar-code hand-held scanner. The hand-held scanner allows XL-MCL flow cytometers to bar-code scan patient samples. The hand-held scanner is pre-programmed to Beckman Coulter’...
Page 158: Verification
INSTALLATION PROCEDURES BAR-CODE HAND-HELD SCANNER OPTION Verification Turn on the power to the Cytometer. After the Computer Workstation boots up into the SYSTEM II software, point the hand-held scanner at a specific area of the XL-MCL flow cytometer and pull the trigger. Ensure that a laser beam image is displayed at the same area of the XL-MCL flow cytometer.
Page 159: 3.13 Serial/Parallel Adapter Interface Card Option
INSTALLATION PROCEDURES SERIAL/PARALLEL ADAPTER INTERFACE CARD OPTION 3.13 SERIAL/PARALLEL ADAPTER INTERFACE CARD OPTION Purpose The purpose of this procedure is to successfully guide you in installing the Serial/Parallel Adapter Interface card. Tools/Supplies Needed B Serial/Parallel Adapter Interface card, PN 2016678 Removal Note: Because of OEM-vendor changes, differences in Computer Workstations and customer-purchased peripheral upgrades, additional installation information may be...
Page 160: Verification
INSTALLATION PROCEDURES SERIAL/PARALLEL ADAPTER INTERFACE CARD OPTION Plug the ac power line cords into appropriate wall outlets and turn the Computer Workstation on. Check to see if any system card conflict messages display on the monitor: If no system card conflict messages display on the monitor go to the next step (6). If system card conflict messages display on the monitor: Repeat steps through 5.
Page 161: Network Setup Using Microsoft Windows 95 Or
INSTALLATION PROCEDURES NETWORK SETUP USING MICROSOFT WINDOWS 95 OR 98 3.14 NETWORK SETUP USING MICROSOFT WINDOWS 95 OR 98 ATTENTION: This procedure is used to connect two or more computers together in Windows 95 or 98 and is designed for customers who are using only EXPO 32 software and do not intend to use the MS-DOS 6.22 and/or MS-DOS mode with their Workstations.
Page 162: Cable Connections
INSTALLATION PROCEDURES NETWORK SETUP USING MICROSOFT WINDOWS 95 OR 98 12. Verify the is present. TCP/IP Protocol If present, go to step 14. If not present, go to step to add the protocol. 13. At the Configuration tab, select the button tt Protocol button tt...
Page 163: Define The Computer Name And Workgroup
INSTALLATION PROCEDURES NETWORK SETUP USING MICROSOFT WINDOWS 95 OR 98 Verify both options on this screen are checked. Repeat this for each Workstation on the network. Define the Computer Name and Workgroup At the Network window, select the Identification tab. Define the Computer Name and Workgroup.
Page 164 INSTALLATION PROCEDURES NETWORK SETUP USING MICROSOFT WINDOWS 95 OR 98 3.14-4 PN 4237029F...
Page 165: Lantastic ® Client/Server Software Option, Version
INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 8.0 ® 3.15 LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 8.0 Purpose This procedure provides instructions for installing LANtastic Client/Server software, version 8.0. These instructions install the current version of LANtastic Client/Server software for either a single-user or a 2 to 10-user license. Tools/Supplies Needed ®...
Page 166 INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 8.0 Select to save the changes. File Save Select File Save As and rename the file from autoexec.bat to autoexec.XXX where the XXX is your initials. This is being done to have a backup of this file in case a problem occurs later.
Page 167 INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 8.0 11. Follow the instructions displayed on the screen to install the Client version of the software. Use the following information to answer the software installation questions when they appear. When the software requests a selection, select the defaults. Do not share drive and printers.
Page 168 INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 8.0 18. Make sure the following lines appear below the REM’d out lines with a blank line between. PATH_C:\SQLANY50\DOS;%PATH% SET_SQLPATH=C:\SQLANY50\DOS SET_SQLANY50=C:\SQLANY50 SET_SQLSTART=C:\SQLANY50\DOS\DBCLIENT_ – x_ netDG_ / q_ / d_ XL2.DB SET_SQLCONNECT=DBA,SQL,, 19. Type any missing lines below the REM’d out lines. Make sure there is a blank line between the lines.
Page 169: Software Installation, Server Setup
INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 8.0 Software Installation, Server Setup ATTENTION: During this installation, no DOS version of the software is installed. Because you set the client computers to not share drivers and printers, you will not be able to see the Client computer from the Server.
Page 170 INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 8.0 Select a Listmode file by changing directories and select W: drive (this is the Server). Again, you may have to re-build the directory. Select any Listmode file or files and then batch play. Ensure that the output option is set to save Listmode and that the patient report and database is enabled.
Page 171: Lantastic ® Client/Server Software Option, Version
INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 7.0 ® 3.16 LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 7.0 Purpose These procedures allow you to reinstall LANtastic Client/Server software V7.0. Tools/Supplies Needed ATTENTION: Needed software must be obtained from the customer. If it is not available, the current version (Version 8.0) must be ordered and installed.
Page 172: Lantastic Client/Server Version For Windows™ 95
INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 7.0 11. From the Windows 95 desktop, select Start Settings Control Panel System Device Manager. 12. From the box, select Device Manager Ports (COM & LPT) 13. Remove each communications port labeled Modemshare (COM3-COM9) 14.
Page 173: Sybase Client/Server Sql Software Option
INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 7.0 11. Change to the c:\lantasti directory, type edit startnet.bat 12. After the line redir..@startnet.cfg, enter the server computer name (name entered in step under heading LANtastic Client/Server Version for Windows™ 95 Installation).
Page 174 INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 7.0 3.16-4 PN 4237029F...
Page 175: Purpose
INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 6.0 ® 3.17 LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 6.0 Purpose These procedures allow you to reinstall LANtastic Client/Server software V6.0. Tools/Supplies Needed ATTENTION: Needed software must be obtained from the customer. If it is not available, the current version (Version 8.0) must be ordered and installed.
Page 176: Config.sys And Startnet.bat File Editing
INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 6.0 10. Ensure all network information is correct before continuing. Press Û. Note: This is the last time you can go back and change information without having to reload the LANtastic Client/Server software. 11.
Page 177 INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 6.0 Press Û and note that the line has changed colors and type: NET USE <drive>:\\<server name>\DRIVE LETTER, and press Û. EXAMPLE: NET USE F: \\NETWORK\D-DRIVE This command tells the Computer Workstation to connect to the file server network’ s D-drive as the Computer Workstation’...
Page 178 INSTALLATION PROCEDURES ® LANtastic CLIENT/SERVER SOFTWARE OPTION, VERSION 6.0 3.17-4 PN 4237029F...
Page 179: Sybase Client/Server Sql Software Option
INSTALLATION PROCEDURES ® Sybase CLIENT/SERVER SQL SOFTWARE OPTION ® 3.18 Sybase CLIENT/SERVER SQL SOFTWARE OPTION Purpose This procedure provides instructions for installing Sybase Client/Server SQL software for use in conjunction with LANtastic Client/Server software. Install LANtastic software before preforming this procedure. Tools/Supplies Needed ®...
Page 180: Software Installation, Server Setup
INSTALLATION PROCEDURES ® Sybase CLIENT/SERVER SQL SOFTWARE OPTION 15. Type editßconfig.sys 16. Type REM in front of the CD-ROM drive statement line. 17. Save the changes. 18. Type editßautoexec.batßfile 19. Remove the REM statement you placed in the CD\XL and XL2 lines. 20.
Page 181: Software And Hardware Validation
INSTALLATION PROCEDURES ® Sybase CLIENT/SERVER SQL SOFTWARE OPTION 11. Close Windows Explorer. 12. Double click on the Sybase icon to start the database. 13. Ensure the database starts and is in the Ready state. 14. Re-boot the Client computer and ensure no errors appear when booting up. Software and Hardware Validation Boot the Server computer into Windows.
Page 182 INSTALLATION PROCEDURES ® Sybase CLIENT/SERVER SQL SOFTWARE OPTION 3.18-4 PN 4237029F...
Page 183: 3.19 Expo32™ Software Options
INSTALLATION PROCEDURES EXPO32™ SOFTWARE OPTIONS 3.19 EXPO32™ SOFTWARE OPTIONS Purpose EXPO32 is a Windows based software package that contains enhancements that make acquisition and data management easier. This software is distributed via CD-ROM and requires Windows 98 Second Edition. The appropriate service ROMLOCK is also required. Tools/Supplies Needed B EXPO32™...
Page 184 INSTALLATION PROCEDURES EXPO32™ SOFTWARE OPTIONS At the Workstation, verify the message Startup in process appears on the status line (lower display area). Note: During system startup, a sequence of messages (Startup in process → Verification → Run Initialization → Awaiting Sample appear as the instrument goes through various checks.
Page 185: Operation
CONTENTS SERVICE AND REPAIR PROCEDURES, 4.1-1 GUIDELINES FOR SERVICING THE INSTRUMENT, 4.1-1 General Guidelines, 4.1-1 Safety Precautions, 4.1-1 Instrument Access, 4.1-1 Laser Safety, 4.1-1 Electronic Precautions, 4.1-1 Procedures, 4.1-2 Tools and Supplies, 4.1-3 Instrument Performance Verification, 4.1-3 Power Down / Power Up Using SYSTEM II™ Software, 4.1-3 Power Down Using SYSTEM II Software, 4.1-3...
Page 186 CONTENTS COVER REMOVAL AND REINSTALLATION, 4.3-1 Purpose, 4.3-1 Overview, 4.3-1 Locating a Procedure, 4.3-1 Exterior Cytometer Covers, 4.3-2 Access Inside the Cytometer, 4.3-3 Power Supply Module Covers, 4.3-4 Procedure 1, 4.3-5 How to Remove and Reinstall the Cytometer Top Cover, 4.3-5 Tools/Supplies Needed, 4.3-5 Removing the Cytometer Top...
Page 187 CONTENTS Procedure 8, 4.3-20 How to Remove and Reinstall the Manual Sample Station, Cytometer without MCL Option, 4.3-20 Tools/Supplies Needed, 4.3-20 Removing the Manual Sample Station on a Cytometer without the MCL Option, 4.3-20 Reinstalling the Manual Sample Station on a Cytometer without the MCL Option, 4.3-22 Procedure 9, 4.3-23...
Page 188: Laser
CONTENTS Procedure 15, 4.3-38 How to Remove and Reinstall the Filter Shield, 4.3-38 Tools/Supplies Needed, 4.3-38 Removing the Filter Shield, 4.3-38 Reinstalling the Filter Shield, 4.3-41 Procedure 16, 4.3-43 How to Remove and Reinstall the Data Acquisition Card Cage, 4.3-43 Tools/Supplies Needed, 4.3-43 Removing the Data Acquisition Card Cage from the Cytometer Center...
Page 189 CONTENTS FLOW CELL AND BEAMSHAPER REPLACEMENT AND/OR ALIGNMENT, 4.5-1 Purpose, 4.5-1 Tools/Supplies Needed, 4.5-1 Preparation, 4.5-1 Flow Cell Replacement, 4.5-2 Beamshaper Assembly Removal, Replacement, and Alignment, 4.5-3 Install Alignment Tools, 4.5-4 Alignment, 4.5-4 Reinstall Covers, 4.5-6 System Verification, 4.5-6 OPTICAL ALIGNMENT, 4.6-1 Purpose, 4.6-1 Tools/Supplies...
Page 190 CONTENTS Verification, 4.10-1 Reinstall Covers, 4.10-1 System Verification, 4.10-1 4.11 SEGMENTING VALVE CLEANING, 4.11-1 Purpose, 4.11-1 Tools/Supplies Needed, 4.11-1 Preparation, 4.11-1 Removal, 4.11-1 Front Segmenting Pad, 4.11-1 Middle Segmenting Pad, 4.11-2 Rear Segmenting Pad, 4.11-2 Clean the Shaft, 4.11-3 Installation, 4.11-3 Rear Segmenting Pad, 4.11-3 Middle Segmenting...
Page 191 CONTENTS Load the Customer’ s Preferred Operating System, 4.14-4 System Verification, 4.14-4 4.15 AMP/SIGNAL CONDITIONER CARD REPLACEMENT AND/OR CALIBRATION, 4.15-1 Purpose, 4.15-1 Tools/Supplies Needed, 4.15-1 If Replacing the Amp/Signal Conditioner Card, 4.15-1 If Calibrating Without Replacing the Amp/Signal Conditioner Card, 4.15-2 Calibration, 4.15-2 Verify Acceptable Histograms, 4.15-4...
Page 192 CONTENTS ±15 Vdc Power Supply, 4.20-4 Preparation, 4.20-4 +15 Vdc Voltage Verification, 4.20-5 -15 Vdc Voltage Verification, 4.20-5 Voltage Adjustment, 4.20-5 MCL Power Supply, 4.20-6 Preparation, 4.20-6 Voltage Verification, 4.20-6 Voltage Adjustment, 4.20-7 4.21 RE-IMAGING FlowCentre™ COMPUTERS, 4.21-1 Purpose, 4.21-1 Tools/Supplies Needed, 4.21-1 Preparation, 4.21-1...
Page 193: Cover
CONTENTS 4.3-14 Cytometer Upper Rear Cover Screw Locations, 4.3-13 4.3-15 Cytometer Lower Rear Cover Screw Locations, 4.3-15 4.3-16 Manual Sample Station Screw Locations, 4.3-18 4.3-17 Manual Sample Station Screw Locations, 4.3-21 4.3-18 Opening the MCL Door, 4.3-23 4.3-19 Closing the MCL Door, 4.3-24 4.3-20 Unlatching the MCL Covers, 4.3-25...
Page 194 CONTENTS 4.20-2 J66 and Pin Locations for +24 Vdc Voltage Verification, 4.20-3 4.23-1 Bar-Code Decoder Card with Component Locations, 4.23-2 TABLES 4.6-1 Oscilloscope Settings, 4.6-1 4.8-1 Carryover Protocol, 4.8-1 4.15-1 LIN/LOG SWITCH POINT 1 TEST Protocol, 4.15-5 4.18-1 Indicator Troubleshooting, 4.18-1 4.20-1 MCL CPU Card, 4.20-6 4.20-2 MCL Power Supply Voltage...
Page 195: Service And Repair Procedures
SERVICE AND REPAIR PROCEDURES GUIDELINES FOR SERVICING THE INSTRUMENT General Guidelines Safety Precautions Review and heed the general safety warnings and cautions listed under Heading 1.2, SAFETY PRECAUTIONS. Make sure all covers are reinstalled to ensure proper operation of interlocks when you leave the account.
Page 196: Procedures
SERVICE AND REPAIR PROCEDURES GUIDELINES FOR SERVICING THE INSTRUMENT WARNING Risk of personal injury or damage to electronic components. While performing service or maintenance on the instrument, rings and other metal jewelry could contact exposed electronic components, causing personal injury from electric shock, or become caught in the instrument, damaging the components.
Page 197: Tools And Supplies
GUIDELINES FOR SERVICING THE INSTRUMENT Tools and Supplies You can do most procedures using the standard Beckman Coulter Service Tool Kit and test equipment (oscilloscope, DVM, and an external digital pressure gauge). Any special tools, supplies, or equipment required are identified under the Tools/Supplies Needed heading at the beginning of the procedure.
Page 198: Stage 2: Power Off The Workstation
SERVICE AND REPAIR PROCEDURES GUIDELINES FOR SERVICING THE INSTRUMENT Stage 2: Power Off the Workstation (system using SYSTEM II software) At the Workstation computer, press and hold the power ON/OFF switch until the computer shuts off. WARNING Risk of personal injury. When servicing components located inside the Power Supply module, a Stage 3: System Power Down must always be performed before removing any cover.
Page 199: Restoring Power To The Cytometer Only
SERVICE AND REPAIR PROCEDURES GUIDELINES FOR SERVICING THE INSTRUMENT At the Cytometer, verify the LASER ON indicator lights green. Note: If the Cytometer turns on and then turns off immediately, check the alarm. (At the Menu bar, select Applications Utilities .
Page 200: Power Down / Power Up Using Expo32™ Adc Software
SERVICE AND REPAIR PROCEDURES GUIDELINES FOR SERVICING THE INSTRUMENT At the Cytometer, verify the CYTOMETER READY indicator lights green. Note: The Cytometer can take up to 30 minutes to warm up before the READY indicator turns green. Power Down / Power Up Using EXPO32™ ADC Software ATTENTION: If the preferred operating system for the instrument you are servicing is SYSTEM II, see the...
Page 201: Stage 3: System Power Down (System Using Expo32 Adc Software)
SERVICE AND REPAIR PROCEDURES GUIDELINES FOR SERVICING THE INSTRUMENT WARNING Risk of personal injury. When servicing components located inside the Power Supply module, a Stage 3: System Power Down must always be performed before removing any cover. Stage 3: System Power Down (system using EXPO32 ADC software) Unplug both ac power line cords from the wall outlet.
Page 202: Restoring Power To The Cytometer Only (System Using Expo32 Adc Software)
SERVICE AND REPAIR PROCEDURES GUIDELINES FOR SERVICING THE INSTRUMENT Restoring Power to the Cytometer Only (system using EXPO32 ADC software) At the Windows Desktop, double click on the EXPO32ADC XL 4 Color icon ( EXPO32ADC icon if this is a 3 color system). XL 3 Color When the box appears, select...
Page 203: Prefinal Service Software Installation And Operation
SERVICE AND REPAIR PROCEDURES PREFINAL SERVICE SOFTWARE INSTALLATION AND OPERATION PREFINAL SERVICE SOFTWARE INSTALLATION AND OPERATION Purpose Use this procedure to install and operate the Prefinal Service software. This software is not intended to be a Pass/Fail for system performance. The SVP must be run after using the Prefinal Service software.
Page 204: If Using Expo32™ Adc Software
SERVICE AND REPAIR PROCEDURES PREFINAL SERVICE SOFTWARE INSTALLATION AND OPERATION If Using EXPO32™ ADC Software Make sure the Workstation computer is turned on. If the Workstation computer is off, power on the Workstation computer and Cytometer, refer to Stage 2: Restoring Power to the Workstation (system using EXPO32 ADC software) under Heading...
Page 205: Operation
SERVICE AND REPAIR PROCEDURES PREFINAL SERVICE SOFTWARE INSTALLATION AND OPERATION Operation At the Prefinal Test menu, use æ and ç to scroll through the menu to the desired option or test. Press Û to access the desired option or test. Table A.4-1 briefly describes the tests available from the Prefinal Test menu.
Page 206: If Expo32 Adc Is The Customer's Preferred Operating System
SERVICE AND REPAIR PROCEDURES PREFINAL SERVICE SOFTWARE INSTALLATION AND OPERATION At the Cytometer, verify the CYTOMETER READY indicator lights green. Perform an SVP as directed under Heading 5.1, SYSTEM VERIFICATION PROCEDURE (SVP) If EXPO32 ADC is the Customer’s Preferred Operating System If you have not already done so, remove the Prefinal Software diskette from the drive.
Page 207: Cover Removal And Reinstallation
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION COVER REMOVAL AND REINSTALLATION Purpose Cover removal and reinstallation is divided into physical sections depending on the area of the instrument you need to access. To expedite finding the appropriate procedure, the procedures in this section are organized into Exterior Cytometer Covers,...
Page 208: Covers
Cytometer cover. WARNING Risk of personal or operator injury. Covers and interlocks are installed on Beckman Coulter instruments to prevent injury from operating components. If you must remove covers or disable interlocks to service an instrument: r Be alert and use extreme care when working around exposed components to avoid personal injury.
Page 209: Access Inside The Cytometer
Cytometer. WARNING Risk of personal or operator injury. Covers and interlocks are installed on Beckman Coulter instruments to prevent injury from operating components. If you must remove covers or disable interlocks to service an instrument: r Be alert and use extreme care when working around exposed components to avoid personal injury.
Page 210: Power Supply Module Covers
WARNING Risk of personal or operator injury. Covers and interlocks are installed on Beckman Coulter instruments to prevent injury from operating components. If you must remove covers or disable interlocks to service an instrument: r Be alert and use extreme care when working around exposed components to avoid personal injury.
Page 211: Procedure 1
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 1 How to Remove and Reinstall the Cytometer Top Cover The Cytometer interlock switch and Data Acquisition card cage are accessible when the Cytometer top cover is removed. The rear section of the optical collection area is also accessible when the Cytometer top cover is removed and the Data Acquisition card cage is removed from the center cavity.
Page 212: Cytometer Interlock
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Cytometer Interlock Switch The Cytometer interlock switch (Figure 4.3-5) is a safety interlock to ensure the Cytometer top cover is in place when the Argon laser is on. If the laser is on and the top cover is removed, this interlock turns off the power to the Argon laser head.
Page 213: Procedure 2
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 2 How to Remove and Reinstall the Cytometer Right-Side Cover Removing the Cytometer right-side cover provides access to the upper pneumatics drawer and the water trap filter, VL33, manifold, and cooling coil mounted on the back of the rear panel.
Page 214: Reinstalling The Cytometer Right-Side
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Reinstalling the Cytometer Right-Side Cover Position the right-side cover on the Cytometer frame. Push the cover down on the Cytometer frame (Figure 4.3-6, item 2). Make sure the upper and lower fasteners on the inside of the cover are properly positioned inside the Cytometer frame.
Page 215: Procedure 3
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 3 How to Remove and Reinstall the Center Front Cover (Filter Cover) Removing the center front cover (as referred to as the filter cover) allows access to the filter shield, the individual light filters, and the ND1 filter positioning knob. See Figure A.5-6 the components that are accessible with the center front cover removed and Table A.5-6...
Page 216: Tools/Supplies
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 4 How to Properly Open and Close the Cytometer Reagent Drawer Opening the reagent drawer allows access to reagent containers, sheath liquid filter, and the purge mechanism. See Figure A.5-8 for the components that are accessible with the reagent drawer open and Table A.5-8 for a description of component functions.
Page 217 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Figure 4.3-11 Opening the Reagent Drawer Grasp the reagent drawer and pull the drawer forward until it stops (Figure 4.3-11). The reagent drawer sits inside self-locking tracks and will stop when it locks. Note: If you need to pull the reagent drawer out further, push and hold the locking tabs...
Page 218: Closing The Reagent
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Closing the Reagent Drawer Figure 4.3-12 Closing the Reagent Drawer Slide the reagent drawer back inside the Cytometer (Figure 4.3-12). Make sure the reagent tubing does not become crimped as you push the drawer closed.
Page 219: Procedure 5
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 5 How to Remove and Reinstall the Cytometer Upper Rear Cover The upper rear cover is only removed when the Argon laser head is replaced. Otherwise, components inside the Cytometer cavity are accessed with the top cover removed and the Data Acquisition card cage lifted and locked in its vertical position and/or with the right-side cover removed.
Page 220: Reinstalling The Cytometer Upper Rear
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Reinstalling the Cytometer Upper Rear Cover Position the upper rear cover on the Cytometer frame. Make sure the labels are on the exterior. Loosely reinstall the 14 Phillips-head screws to secure the cover to the Cytometer frame. Figure 4.3-14 for screw locations.
Page 221: Procedure 6
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 6 How to Remove and Reinstall the Cytometer Lower Rear Cover The Cytometer lower rear cover rarely needs to be removed. When access to this lower rear area is required, it is generally easier to gain that access by removing either the left-side or right-side cover.
Page 222: Reinstalling The Cytometer Lower Rear
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Gently pull the cover away from the Cytometer frame. Any tubings or cables that restrict access must be removed. Make sure you are certain where to reconnect each tubing or cable you disconnect. If later, the reconnection location may be unclear, write the reconnection location on a piece of paper and wrap the label around the disconnected tubing or cable to ensure proper reconnection.
Page 223: Procedure 7
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 7 How to Remove and Reinstall the Manual Sample Station, Cytometer with MCL Option The manual sample station must be removed to access the segmenting valve. To access the lower pneumatics drawer, the manual sample station must be removed to access one of the Phillips-head screws that secure the MCL to the Cytometer main frame.
Page 224 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Remove the manual sample station cover assembly: Locate and remove the two pan-head screws that secure the left side of the manual sample station to the Cytometer frame (Figure 4.3-16). You may need to move the MCL further away from the Cytometer frame.
Page 225: Reinstalling The Manual Sample Station On A Cytometer With The Mcl Option Installed
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Reinstalling the Manual Sample Station on a Cytometer with the MCL Option Installed If you removed the sample and waste connect panel, reinstall the panel using the two Phillips-head screws removed earlier. Reinstall the manual sample station cover assembly: Position the manual sample station cover assembly back on the Cytometer frame.
Page 226: Procedure 8
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 8 How to Remove and Reinstall the Manual Sample Station, Cytometer without MCL Option The manual sample station must be removed to access the segmenting valve. See Figure A.5-11 for manual sample station components and Table A.5-11 for a description of component functions.
Page 227 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Figure 4.3-17 Manual Sample Station Screw Locations Remove screws Remove screws 7029244F Pull the manual sample station cover assembly off the Cytometer frame and lay the assembly on top of the reagent containers. Make sure the flat ribbon cable is positioned so that it does not become damaged.
Page 228 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Reinstalling the Manual Sample Station on a Cytometer without the MCL Option If you removed the sample and waste connect panel, reinstall the panel using the two Phillips-head screws removed earlier. Reinstall the manual sample station cover assembly: Position the manual sample station cover assembly back on the Cytometer frame.
Page 229: Procedure 9
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 9 How to Open and Close the MCL Door The MCL door (upper cover) is opened routinely to either place a carousel on the carousel hub (also referred to as the indexing hub) or remove a carousel. The MCL door must be closed during operation.
Page 230 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION WARNING Risk of personal injury. Be very careful when operating the instrument when the MCL interlock switch is defeated, as you may be exposed to moving components. After servicing the instrument, make sure the MCL door is properly closed to reactivate the safety interlock switch if it was bypassed while servicing the instrument.
Page 231 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 10 How to Unlatch and Relatch the MCL Covers The MCL must be unlatched, but not removed, before removing the left-side cover or manual sample station. See Figure A.5-13 for MCL carousel components and Table A.5-13 for a description of component functions.
Page 232 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Relatching the MCL Covers Note: The MCL base may be relatched with the door opened or closed. If the MCL door is open, the rear latch catch and keeper can be viewed through the opening for the gas cylinder. Align the latch catches on the MCL lower cover with the latch keepers attached to left-side of the Cytometer frame (Figure...
Page 233 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 11 How to Remove and Reinstall MCL Covers For most procedures, the MCL can simply be unlatched from the Cytometer frame. The MCL covers must be removed to access the MCL carousel base assembly or the lower pneumatics drawer.
Page 234 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Push down on the air cylinder and move it out of the way of the cover (Figure 4.3-22, item 5). Grasp the lower base of the MCL. Place your right hand on the front of the base near the Cytometer and your left hand at the rear corner.
Page 235 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Reinstalling the MCL Covers Position the MCL covers over the MCL carousel base assembly. Connect P17 to J17 (Figure 4.3-23, item 7). Firmly push the lower base back on latch attached to the Cytometer frame (Figure 4.3-23, item 6).
Page 236 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 12 How to Remove and Reinstall the MCL Probe Housing The MCL probe housing cover must be removed before the left-side cover or manual sample station can be removed. See Figure A.5-13 for MCL carousel components (including the MCL probe housing) and Table A.5-13...
Page 237 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Removing the MCL Probe Housing Cover Push the button to unlock the MCL door (Figure 4.3-25, item 1). The door pops open as the air cylinder attached to the door extends. Figure 4.3-25 Opening the MCL Door Push to Interlock open cover...
Page 238 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Remove the two Phillips-head screws that secure the MCL probe housing cover to the left-side cover (Figure 4.3-26). WARNING Risk of personal injury. Removing the MCL probe housing cover exposes you to the bar-code reader laser beam.
Page 239 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Reinstalling the MCL Probe Housing Cover If the MCL door is blocking the area, push the button to unlock the MCL door then push the door up to ensure it is fully open. Refer to Figure 4.3-25 as needed.
Page 240 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 13 How to Remove and Reinstall the Cytometer Left-Side Cover (Cytometer with MCL Option Installed) Removing the Cytometer left-side cover provides access to the MCL components attached to the MCL main frame. Removing the MCL main frame provides access to the lower pneumatics drawer.
Page 241 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Remove the MCL probe housing (Figure 4.3-28, item 2). Push the button to unlock the MCL door. The door pops open as the air cylinder attached to the door extends. Push the door up to ensure it is fully open. Remove the two Phillips-head screws securing the housing to the Cytometer frame.
Page 242 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 14 How to Remove and Reinstall the Cytometer Left-Side Cover (Cytometer without MCL Option) Removing the Cytometer left-side cover provides access to the lower pneumatics drawer. See Figure A.5-15 for lower pneumatics drawer components and Table A.5-15 for a description of component functions.
Page 243 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Reinstalling the Cytometer Left-Side Cover (Cytometer without MCL Option) Position the left-side cover on the Cytometer frame. Push the cover down on the Cytometer frame (Figure 4.3-29, item 2). Make sure the upper and lower fasteners on the inside of the cover are properly positioned inside the Cytometer frame.
Page 244 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 15 How to Remove and Reinstall the Filter Shield The filter shield covers the sensing area that houses the flow cell, beam-shaping hardware, FS detector, and side scatter diode. The filter shield must be removed to replace these components or when aligning the optics or laser.
Page 245 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Remove the manual sample station cover assembly. Pull the reagent drawer out further. The reagent drawer sits inside self-locking tracks. Push and hold the locking tabs under the tracks and pull the drawer forward.
Page 246 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION 11. Lift the front panel display cover and place a long screwdriver (with a plastic handle) in the upper frame channel to brace the cover open (Figure 4.3-30). Figure 4.3-30 Use a Long Screwdriver to Brace the Upper Cover Open Cytometer upper cover (open)
Page 247 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION 13. Set the filter shield aside in a safe place where it will not be damaged. Reinstalling the Filter Shield Position the filter shield over the sensing area and secure it to the Cytometer frame using the four Phillips-head screws removed earlier (Figure 4.3-31).
Page 248 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION 13. Reinstall the MCL probe housing using the two screws removed earlier. Open the MCL door then push the door up to ensure it is fully open. Make sure the back edge of the MCL probe housing cover is aligned to slide under the edge of the left-side cover then slide the beveled edge of the housing cover under the notched opening at the top of the push-button panel for the MCL door.
Page 249 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 16 How to Remove and Reinstall the Data Acquisition Card Cage The Data Acquisition card cage is accessible when the Cytometer top cover is removed. However, most procedures require the card cage be lifted out of the Cytometer and locked in its vertical position.
Page 250 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Figure 4.3-32 Data Acquisition Card Cage Locked in its Vertical Position Data Acquisition card cage (locked in vertical position) 7029255F Note: Once the card cage is locked in the vertical position, power may be restored to the Cytometer as needed.
Page 251 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Reinstalling the Data Acquisition Card Cage Back in the Cytometer Center Cavity Power off the Cytometer, if applicable. Refer to Stage 1: Power Off the Cytometer (system using SYSTEM II software) under Heading 4.1, as needed.
Page 252: How To Remove And Reinstall The Lower Pneumatics Drawer
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 17 How to Remove and Reinstall the Lower Pneumatics Drawer The lower pneumatics drawer contains the waste chamber, the Solenoid Power Distribution card as well as most of the solenoids and pinch valves used in the Cytometer. The lower pneumatics drawer is located in the left-side the Cytometer underneath the optical bench and can be easily accessed on an XL flow cytometer that does not have the MCL option installed.
Page 253 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Firmly pull the lower base away from the Cytometer frame (Figure 4.3-23, item 6). Disconnect P17 from J17 (Figure 4.3-23, item 7). m. Set the MCL covers aside in a safe place where it will not be damaged. Slide the reagent drawer open.
Page 254 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Figure 4.3-33 Removing the MCL from the Cytometer Frame Remove screws Remove screws Long Phillips-head screwdriver 7029236F Locate the lower pneumatics drawer (Figure 4.3-34). Figure 4.3-34 Removing the Lower Pneumatics Drawer (Cytometer with the MCL Option Installed) Captive thumbscrews 7029242F...
Page 255: Removing The Lower Pneumatics Drawer On A Cytometer Without The Mcl Option
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Removing the Lower Pneumatics Drawer on a Cytometer without the MCL Option Remove the Cytometer top cover. At the rear of the Cytometer, remove the four Phillips-head screws securing the top cover to the Cytometer (Figure 4.3-29, item 1).
Page 256: Reinstalling The Lower Pneumatics Drawer
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Reinstalling the Lower Pneumatics Drawer Push the lower pneumatics drawer back inside the Cytometer. Tighten the captive thumbscrews to reattach the lower pneumatics drawer to the Cytometer frame. Use instrument specific instructions to proceed: If this is an XL cytometer with the MCL option, go to step 4.
Page 257 SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION 17. Reinstall the carousel hub on the MCL base and secure it with the Phillips-head screw removed earlier Figure 4.3-22, item 3). 18. Place the carousel back on the carousel hub, if desired. 19.
Page 258: Procedure 18
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 18 How to Remove and Reinstall the Upper Pneumatics Drawer EMC Shield The upper pneumatics drawer contains many components including the Sensor card, the sheath pressure regulator, sample pressure regulator, vacuum regulator, sensors, and pinch valves needed for providing and maintaining proper sheath flow and sample flow.
Page 259: Reinstalling The Upper Pneumatics Drawer Emc Shield
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Remove the four Phillips-head screws that secure the EMC shield to the upper pneumatics drawer cabinet. There are two screws securing the top and two screws securing the bottom. See Figure 4.3-36, item 3. Pull the EMC shield off the drawer.
Page 260: Procedure 19
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 19 How to Remove and Reinstall the Power Supply Module Three-Side Cover The Power Supply module provides and monitors the main electronic and pneumatic power (vacuum and pressure) to the Cytometer. Figure A.6-3 for the main components located in the left side and Table A.6-3...
Page 261: Reinstalling The Power Supply Module's Three-Sided Cover
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Figure 4.3-37 Interlock Switch Location, Power Supply Module Interlock switch 7029253F WARNING Risk of personal injury. Be very careful when operating the instrument when the safety interlock switch in the Power Supply module is defeated, as you may be exposed to electric shock. After servicing the instrument, make sure the three-sided cover is properly reinstalled to reactivate the safety interlock switch if it was bypassed while servicing the instrument.
Page 262: Procedure 20
SERVICE AND REPAIR PROCEDURES COVER REMOVAL AND REINSTALLATION Procedure 20 How to Open and Close the Power Supply Module Front Door The Power Supply module provides and monitors the main electronic and pneumatic power (vacuum and pressure) to the Cytometer. See Figure A.6-2 for the components located behind the front door and...
Page 263: Argon Laser Head Or Power Supply Replacement
Laser Current Error or Laser Current Warning message Laser Power Error or Laser Power Warning message Tools/Supplied Needed B Standard Beckman Coulter tool kit, PN 5415102 B 5/32 in., T-handle, Allen wrench B Argon air-cooled laser head, PN 7000358 B Argon air-cooled laser switching power supply, as applicable...
Page 264: Installation
SERVICE AND REPAIR PROCEDURES ARGON LASER HEAD OR POWER SUPPLY REPLACEMENT Remove the four Phillips-head mounting screws securing the Argon laser power supply to the Power Supply module. Remove the defective Argon laser power supply from the Power Supply module. Installation Position the replacement Argon laser power supply in the Power Supply module.
Page 265: Installation
SERVICE AND REPAIR PROCEDURES ARGON LASER HEAD OR POWER SUPPLY REPLACEMENT Installation Carefully route the replacement laser head (and attached laser umbilical cord) through the top of the Cytometer and position the laser head on the optical bench. Secure the laser head to the optical bench using four countersunk Allen-head screws. Secure the laser beam shield using two Phillips-head screws.
Page 266 SERVICE AND REPAIR PROCEDURES ARGON LASER HEAD OR POWER SUPPLY REPLACEMENT 4.4-4 PN 4237029F...
Page 267: Flow Cell And Beamshaper Replacement And/Or Alignment
SERVICE AND REPAIR PROCEDURES FLOW CELL AND BEAMSHAPER REPLACEMENT AND/OR ALIGNMENT FLOW CELL AND BEAMSHAPER REPLACEMENT AND/OR ALIGNMENT Purpose Use this procedure after replacing the: Flow cell Beamshaper Laser Lens holders. This procedure is performed with the unit on and all covers removed. If replacing the laser, first follow the procedures as directed under Heading 4.4, ARGON LASER HEAD OR POWER SUPPLY...
Page 268: Flow Cell Replacement
SERVICE AND REPAIR PROCEDURES FLOW CELL AND BEAMSHAPER REPLACEMENT AND/OR ALIGNMENT Figure 4.5-1 Flow Cell and Beamshaper Replacement Components OPTICAL HOUSING FOOT BEAMSHAPER OPTICAL ASSEMBLY HOUSING LASER BRACKET FS DETECTOR FLOW CELL 7029082D Flow Cell Replacement Remove the tubing attached to the flow cell. Label the tubing for correct reattachment later.
Page 269: Beamshaper Assembly Removal, Replacement, And Alignment
SERVICE AND REPAIR PROCEDURES FLOW CELL AND BEAMSHAPER REPLACEMENT AND/OR ALIGNMENT WARNING The laser beam can cause eye damage if the laser beam’s path is viewed directly on the same viewing plane. Avoid direct exposure to beam. Do not view directly or with optical instruments except for special service instruments as directed in this service manual.
Page 270: Install Alignment Tools
SERVICE AND REPAIR PROCEDURES FLOW CELL AND BEAMSHAPER REPLACEMENT AND/OR ALIGNMENT Install Alignment Tools Install the 10-mm stage clamp to the 10-mm stage and secure it by tightening the 1/8 in. Allen setscrew (Figure 4.5-3). Figure 4.5-3 Beamshaper Components BEAMSHAPER CLAMP SETSCREW BEAMSHAPER...
Page 271 SERVICE AND REPAIR PROCEDURES FLOW CELL AND BEAMSHAPER REPLACEMENT AND/OR ALIGNMENT Figure 4.5-4 Flow Cell and Beamshaper Alignment 10-mm LENS BENDING TOOL ADJUSTMENT 80-mm LENS HOLDER 10-mm LENS 10-mm LENS HOLDER 7029074D Carefully turn the bending tool to get the correct laser image to appear on the Mylar flexible film (Figure 4.5-5).
Page 272: Reinstall Covers
SERVICE AND REPAIR PROCEDURES FLOW CELL AND BEAMSHAPER REPLACEMENT AND/OR ALIGNMENT Reinstall Covers Reinstall all covers removed during this procedure. Refer to Heading 4.3, COVER REMOVAL AND REINSTALLATION if you need detailed instructions System Verification Perform an SVP as directed under Heading 5.1, SYSTEM VERIFICATION PROCEDURE (SVP).
Page 273: Optical Alignment
SERVICE AND REPAIR PROCEDURES OPTICAL ALIGNMENT OPTICAL ALIGNMENT Purpose Perform this procedure whenever a Preventative Maintenance Inspection (PMI) procedure is performed or any of the following are replaced: The laser The flow cell The FS detector. Tools/Supplies Needed B One, empty, 12 x 75-mm test tube, PN 2523749 B Flow-Check fluorospheres, PN 6605359 B 7/64 in., T-handle, Allen wrench B 7/64 in., L-handle, Allen wrench...
Page 274: Forward Scatter (Fs) Mask Alignment
SERVICE AND REPAIR PROCEDURES OPTICAL ALIGNMENT Using the focus adjustment knob (Figure 4.6-1), adjust the beam-shaping assembly until the narrowest pulse width for FS is observed. Figure 4.6-1 Beam-Shaping Stage Components FLUORESCENCE LENS ADJUSTMENT FLOW CELL FOCUS ADJUSTMENT KNOB BEAM UP/DOWN ADJUSTMENT Loosen these screws to slide...
Page 275 SERVICE AND REPAIR PROCEDURES OPTICAL ALIGNMENT Remove the FALS Hybrid Detector card mounted to the FS detector assembly (Figure 4.6-2). Figure 4.6-2 Forward Scatter (FS) Movable Mask Adjustment FS DETECTOR ASSEMBLY FALS HYBRID DETECTOR CARD FS MOVABLE MASK ADJUSTMENT ARM Loosen screws to adjust mask.
Page 276: How To Proceed
SERVICE AND REPAIR PROCEDURES OPTICAL ALIGNMENT Figure 4.6-3 Correct/Incorrect Scattered Light Positioning CORRECT INCORRECT ALIGNMENT ALIGNMENT ADJUSTMENT WHITE PAPER Tighten screws when mask aligned. 7029081D 10. When the mask is properly aligned, tighten the screws (Figure 4.6-3). How to Proceed If you did this procedure as part of the Cytometer installation, go back to Heading 3.7 and complete the procedures under the...
Page 277: Sample And Sheath Sensor Calibration
SERVICE AND REPAIR PROCEDURES SAMPLE AND SHEATH SENSOR CALIBRATION SAMPLE AND SHEATH SENSOR CALIBRATION Purpose Perform this procedure if erratic data rates exist or if you replace any of the following: System Interface card Sensor card Sample regulator (electronic regulator) Sheath regulator.
Page 278: Calibration
SERVICE AND REPAIR PROCEDURES SAMPLE AND SHEATH SENSOR CALIBRATION At the System Interface card: Connect the positive DVM lead to TP3 and, as needed, adjust the SAMPLE ZERO potentiometer until the DVM reads 0.000 ±0.001 Vdc. For test point and potentiometer locations, refer to Figure A.2-19 as needed.
Page 279 SERVICE AND REPAIR PROCEDURES SAMPLE AND SHEATH SENSOR CALIBRATION Disconnect one of the PMT ± 15 Vdc power connectors and connect the four-pin power connector from the pneumatic calibration box in its place. To locate these connectors, refer to Figure A.2-14 as needed.
Page 280: Operational Verification
SERVICE AND REPAIR PROCEDURES SAMPLE AND SHEATH SENSOR CALIBRATION 32. In the upper pneumatics drawer, adjust the vacuum regulator (RG3) to 7.00 inches. To locate the vacuum regulator, refer to Figure A.5-5 as needed. 33. Remove the external vacuum gauge. 34.
Page 281: Reinstall Assemblies And Covers
SERVICE AND REPAIR PROCEDURES SAMPLE AND SHEATH SENSOR CALIBRATION Run five test tubes containing 1.0 mL of Flow-Check fluorospheres. Fill five test tubes to the 1.0 mL mark with Flow-Check fluorospheres. Place a test tube into the manual sample station. Run the sample.
Page 282 SERVICE AND REPAIR PROCEDURES SAMPLE AND SHEATH SENSOR CALIBRATION 4.7-6 PN 4237029F...
Page 283: Carryover Test
SERVICE AND REPAIR PROCEDURES CARRYOVER TEST CARRYOVER TEST Purpose This test verifies sample carryover in the sample tubing and flow cell area. Perform this test if the customer complains of sample carryover. If an XL-MCL flow cytometer is being checked, the carryover test must be performed using the manual sample station then repeated using the MCL sample station.
Page 284: Procedure
SERVICE AND REPAIR PROCEDURES CARRYOVER TEST Procedure Using the Manual Sample Station Select the CARRYOVER protocol from the protocol screen. Insert the test tube labeled BEADS in the manual sample station. Run the CARRYOVER protocol. At the end of the run, record the time it took to reach 10,000 events and save the printout.
Page 285 SERVICE AND REPAIR PROCEDURES CARRYOVER TEST 10. Wait five minutes. 11. After the five minute wait, place another ISO test tube in the MCL carousel. 12. Rerun the CARRYOVER protocol with a manual stop at the time recorded in step 4. 13.
Page 286 SERVICE AND REPAIR PROCEDURES CARRYOVER TEST 4.8-4 PN 4237029F...
Page 287: Peek Tubing Replacement
Beckman Coulter recommends that you wear barrier protection, such as appropriate safety glasses, a lab coat and latex gloves throughout the performance of service, maintenance and troubleshooting procedures to avoid contact with biohazardous material in the instrument.
Page 288 SERVICE AND REPAIR PROCEDURES PEEK TUBING REPLACEMENT Figure 4.9-1 PEEK Tubing Connections PEEK TUBING PEEK TUBING TO MANUAL PEEK TUBING TO FLOW CELL TO MCL PICKUP PROBE FRONT SEGMENTING PAD REAR SEGMENTING PAD 7029063D Figure 4.9-2 PEEK Tubing with Ferrule and Ferrule Nut Ferrule Ferrule Nut PEEK tubing...
Page 289: Installation
SERVICE AND REPAIR PROCEDURES PEEK TUBING REPLACEMENT Slide the segmenting valve forward on the shaft until the black ferrule nuts are accessible. Note: Two ferrule nuts are present. If the MCL option is installed, blue PEEK tubing extends from each ferrule nut. The ferrule nut to your left is associated with the manual sample probe;...
Page 290 SERVICE AND REPAIR PROCEDURES PEEK TUBING REPLACEMENT Using the shorter piece of blue PEEK tubing: Thread a ferrule nut (knurled end first) followed by a ferrule (tapered end first) onto one end of the tubing. See Figure 4.9-2 for correct orientation of components. ATTENTION: Install the new PEEK tubing with the ferrule pushed back (Figure...
Page 291: Reinstall Covers
SERVICE AND REPAIR PROCEDURES PEEK TUBING REPLACEMENT 15. Press the PRIME button and observe the segmenting valve. Make sure the segmenting valve pads are rotating smoothly and there is no leakage at the segmenting valve, flow cell, or sample probe(s). Reinstall Covers Reinstall the sample and waste connect panel using the two Phillips-head screws removed earlier.
Page 292 SERVICE AND REPAIR PROCEDURES PEEK TUBING REPLACEMENT 4.9-6 PN 4237029F...
Page 293: 4.10 Sheath Pressure/Sample Station Vacuum Calibration
SERVICE AND REPAIR PROCEDURES SHEATH PRESSURE/SAMPLE STATION VACUUM CALIBRATION 4.10 SHEATH PRESSURE/SAMPLE STATION VACUUM CALIBRATION Purpose This procedure provides instructions for field calibration of the sheath pressure regulator and the sample station vacuum regulator. Follow this procedure if either regulator is erratic or replaced.
Page 294 SERVICE AND REPAIR PROCEDURES SHEATH PRESSURE/SAMPLE STATION VACUUM CALIBRATION 4.10-2 PN 4237029F...
Page 295: 4.11 Segmenting Valve Cleaning
Beckman Coulter recommends that you wear barrier protection, such as appropriate safety glasses, a lab coat and latex gloves throughout the performance of service, maintenance and troubleshooting procedures to avoid contact with biohazardous material in the instrument.
Page 296: Middle Segmenting Pad
SERVICE AND REPAIR PROCEDURES SEGMENTING VALVE CLEANING To facilitate installation, label the positions where the tubing is connected to the front segmenting pad (Figure 4.11-2). Figure 4.11-2 Segmenting Valve Pad Connections FITTING FITTING ORANGE STRIPE TO MCL TO MANUAL TUBING (WASTE) FITTING TO PICKUP PROBE PICKUP PROBE...
Page 297: Clean The Shaft
SERVICE AND REPAIR PROCEDURES SEGMENTING VALVE CLEANING If the MCL option is installed, unscrew the second black ferrule nut and remove the nut (with attached PEEK tubing and ferrule) from the rear pad. Make sure the ferrule is not lost. If the MCL option is not installed, unscrew and remove the black knurled plug and set it aside.
Page 298: Front Segmenting Pad
SERVICE AND REPAIR PROCEDURES SEGMENTING VALVE CLEANING Front Segmenting Pad Remove the front segmenting pad from the bleach and rinse it with distilled water. Connect the tubing to the front segmenting pad (Figure 4.11-2): Orange-striped Y-tubing White-striped Y-tubing Orange-striped (WASTE) tubing. Install the front pad on the shaft.
Page 299: 4.12 Mcl Option Field Adjustment
After field replacement Upon detecting erratic MCL option operation. Tools/Supplies Needed B COULTER EPICS XL/XL-MCL Prefinal Software diskette, PN 7231244 B Five, empty, 12 x 75-mm test tubes, PN 2523749 Preparation Remove all covers required to access the MCL main frame assembly. Refer to Heading 4.3, COVER REMOVAL AND REINSTALLATION...
Page 300: Carousel Tube-Position Sensor Adjustment
SERVICE AND REPAIR PROCEDURES MCL OPTION FIELD ADJUSTMENT Carousel Tube-Position Sensor Adjustment Insert a carousel with five test tubes installed in the first five carousel positions. Press È, then type CH. The carousel rotates to the home position. Press È, then type CI. The carousel moves in towards the MCL option probe. Press È, then type CR.
Page 301: Finger Adjustment
SERVICE AND REPAIR PROCEDURES MCL OPTION FIELD ADJUSTMENT Figure 4.12-2 Carousel Tube-Position Screw Adjustment CAROUSEL LIFTER FOOT (CENTERED) ADJUST 7029065D Press È, then type LD. The lifter lowers. 10. Press È, then type CO. The carousel moves out. 11. Press È, then type CL. The carousel moves back to the load position. 12.
Page 302: Mcl Sample Probe Adjustment
SERVICE AND REPAIR PROCEDURES MCL OPTION FIELD ADJUSTMENT MCL Sample Probe Adjustment Insert a carousel with one test tube in the first carousel position. Press È, then type CH. The carousel rotates to the home position. Press È, then type CI. The carousel moves in towards the MCL option probe. Press È, then type CR.
Page 303: 4.13 Vortexer Foot Assembly Replacement
SERVICE AND REPAIR PROCEDURES VORTEXER FOOT ASSEMBLY REPLACEMENT 4.13 VORTEXER FOOT ASSEMBLY REPLACEMENT Purpose Perform this procedure to replace the vortex foot If the bearing is binding. To determine if the vortex foot and bearing need to be replaced, rotate the foot clockwise/counter clockwise and ensure that it spins freely without resistance.
Page 304: Installation
SERVICE AND REPAIR PROCEDURES VORTEXER FOOT ASSEMBLY REPLACEMENT Figure 4.13-1 Vortex Foot Assembly, Location and Components Vortex shaft Vortex cover 7029256F Remove the four screws securing the vortex cover (Figure 4.13-1). Remove the two screws securing the vortex shaft to the eccentric slide mechanism. Remove the defective vortexer foot assembly.
Page 305: Reinstall Covers
SERVICE AND REPAIR PROCEDURES VORTEXER FOOT ASSEMBLY REPLACEMENT Reinstall Covers Reinstall all covers removed during this procedure. Refer to Heading 4.3, COVER REMOVAL AND REINSTALLATION if you need detailed instructions. Verification Power on the Cytometer. Refer to Restoring Power to the Cytometer Only (system using SYSTEM II software) under Heading 4.1, as needed.
Page 306 SERVICE AND REPAIR PROCEDURES VORTEXER FOOT ASSEMBLY REPLACEMENT 4.13-4 PN 4237029F...
Page 307: 4.14 Trans Data Acquisition Card Replacement And/Or Calibration
Acquisition card offset when the offset of an Amp/Signal Conditioner card is out of tolerance. Calibration of a Trans Data Acquisition card may also be referred to as offset adjustment. Tools/Supplies Needed B COULTER EPICS XL/XL-MCL Prefinal Software diskette, PN 7231244 B XL card extender, PN 6705582 B Hardcopy of Figure A.2-21, Trans Data Acquisition Card - Component...
Page 308: If Calibrating Without Replacing The Trans Data Acquisition Card
SERVICE AND REPAIR PROCEDURES TRANS DATA ACQUISITION CARD REPLACEMENT AND/OR CALIBRATION If Calibrating Without Replacing the Trans Data Acquisition Card Remove the Trans Data Acquisition card from the Data Acquisition card cage. Remove the jumper from E1 to E2 and install it on E2 to E3. For jumper locations, refer Figure A.2-21 as needed.
Page 309 SERVICE AND REPAIR PROCEDURES TRANS DATA ACQUISITION CARD REPLACEMENT AND/OR CALIBRATION If the reading is out of tolerance, the calibration must be repeated as follows: Press È to exit ADC ZERO ADJUST Press ß to power off the Cytometer. Verify the Cytometer power is off. (Pneumatics should be off and the front panel display should be dark.) Remove the Trans Data Acquisition card from the Data Acquisition card cage.
Page 310: Reinstall Cover
SERVICE AND REPAIR PROCEDURES TRANS DATA ACQUISITION CARD REPLACEMENT AND/OR CALIBRATION For each parameter, verify the offset voltage reading is displayed in green. Select the desired parameter using the cursor keys, æ or ç. All the offset voltages must be displayed in green. If you are testing a 3-color system, the PMT4 parameter fails because there is no circuit card in the card cage.
Page 311: 4.15 Amp/Signal Conditioner Card Replacement And/Or Calibration
Cytometer. Calibration of an Amp/Signal Conditioner card may also be referred to as offset adjustment. Tools/Supplies Needed B COULTER EPICS XL/XL-MCL Prefinal Software diskette, PN 7231244 B One, empty, 12 x 75-mm test tube, PN 2523749 B Isoflow sheath fluid, PN 8547008...
Page 312: If Calibrating Without Replacing The Amp/Signal Conditioner Card
SERVICE AND REPAIR PROCEDURES AMP/SIGNAL CONDITIONER CARD REPLACEMENT AND/OR CALIBRATION On the replacement Amp/Signal Conditioner card: Verify the jumper settings using Figure A.2-1 as a reference. Remove the jumper from E4 to E5. Save the jumper. For jumper locations, refer to Figure A.2-1 as needed.
Page 313 SERVICE AND REPAIR PROCEDURES AMP/SIGNAL CONDITIONER CARD REPLACEMENT AND/OR CALIBRATION If the reading is out of tolerance: Press È to exit ADC ZERO ADJUST Press ß to power off the Cytometer. Verify the Cytometer power is off. (Pneumatics should be off and the front panel display should be dark.) Replace the circuit card as instructed under Heading If Replacing the...
Page 314: Verify Acceptable Histograms
SERVICE AND REPAIR PROCEDURES AMP/SIGNAL CONDITIONER CARD REPLACEMENT AND/OR CALIBRATION Go to the If Calibrating Without Replacing the Amp/Signal Conditioner Card heading and perform the calibration as written. Note: If you have already readjusted the circuit card and the offset reading is still out of tolerance, the Amp/Signal Conditioner card is defective.
Page 315 SERVICE AND REPAIR PROCEDURES AMP/SIGNAL CONDITIONER CARD REPLACEMENT AND/OR CALIBRATION Create a protocol called LIN/LOG SWITCH POINT 1 TEST according to Table 4.15-1 and the setting information below this table. Table 4.15-1 LIN/LOG SWITCH POINT 1 TEST Protocol Histogram Parameter Signals Gain Gating...
Page 316: Reinstall Cover
SERVICE AND REPAIR PROCEDURES AMP/SIGNAL CONDITIONER CARD REPLACEMENT AND/OR CALIBRATION If all histograms are free of gaps or spikes at Channel 28, go to the Reinstall Cover heading that follows. If one or more histograms exhibit gaps or spikes at Channel 28: Power off the Cytometer.
Page 317: 4.16 Preparing Instrument For Shipment
Tools/Supplies Needed B Bleach (household, unscented) B One, empty, 12 x 75-mm test tube, PN 2523749 B COULTER EPICS XL/XL-MCL Prefinal Software diskette, PN 7231244 B 1 gal. deionized or distilled water (found in most laboratories) Procedure Blood can clog lines. If fluids are not cleaned from the lines after use, clogging can ATTENTION: occur.
Page 318 SERVICE AND REPAIR PROCEDURES PREPARING INSTRUMENT FOR SHIPMENT 18. Power down the entire system. (This includes unplugging both ac power line cords from the wall outlet.) If SYSTEM II is the preferred operating system, under Heading 4.1, complete the three stages detailed under the Power Down Using SYSTEM II Software heading.
Page 319: 4.17 Instrument Shutdown
SERVICE AND REPAIR PROCEDURES INSTRUMENT SHUTDOWN 4.17 INSTRUMENT SHUTDOWN Purpose Ensure that you go through this procedure once with the customer before leaving the site. Tools/Supplies Needed B One, empty, 12 x 75-mm test tube, PN 2523749 B IsoFlow sheath fluid, PN 8547008 Procedure With the Cytometer in the Run mode and the Computer Workstation in the Acquisition Halted mode, remove the sample tube from the sample station.
Page 320 SERVICE AND REPAIR PROCEDURES INSTRUMENT SHUTDOWN 4.17-2 PN 4237029F...
Page 321: 4.18 Three-Wire Circuit Analyzer Test
SERVICE AND REPAIR PROCEDURES THREE-WIRE CIRCUIT ANALYZER TEST 4.18 THREE-WIRE CIRCUIT ANALYZER TEST Purpose This test allows you to determine if the standard 115 Vac receptacle (NEMA 5-15R or NEMA 5-20R) has electrical wiring faults. The 3-wire circuit analyzer is not however, a comprehensive instrument.
Page 322: Reverse/Shorted Ground/Neutral Test
SERVICE AND REPAIR PROCEDURES THREE-WIRE CIRCUIT ANALYZER TEST Reverse/Shorted Ground/Neutral Test ATTENTION: If the receptacle passes the THREE-WIRE CIRCUIT ANALYZER TEST, you must perform the reverse/shorted ground/neutral test as described in the following procedure. Tools/Supplies Needed B 3-wire circuit analyzer, PN 2906883 Procedure Place the 3-wire circuit analyzer’...
Page 323: 4.19 Power Supply Replacement
These power supplies are located in the right side compartment of the Power Supply module. Tools/Supplies Needed B Beckman Coulter Service Tool Kit, PN 5415102 B +5 Vdc Power Supply, PN 7000356 B ± 15 Vdc Power Supply, PN 7000355 B +24 Vdc Power Supply, PN 7000357 B MCL Power Supply assembly (+5 Vdc, ±...
Page 324: Removal
SERVICE AND REPAIR PROCEDURES POWER SUPPLY REPLACEMENT Removal Locate the power supply that needs replaced. For power supply locations, refer to Figure A.6-4, as needed. Remove the defective power supply. Replacement Install the replacement power supply. Plug the Power Supply module’ s ac power line cords into the appropriate wall outlets. Wait 15 minutes to allow the Power Supply module to warm up.
Page 325: 4.20 Power Supply Voltage Verification And Adjustment
SERVICE AND REPAIR PROCEDURES POWER SUPPLY VOLTAGE VERIFICATION AND ADJUSTMENT 4.20 POWER SUPPLY VOLTAGE VERIFICATION AND ADJUSTMENT Purpose Perform this procedure to verify (and adjust if needed) voltages at the following power supplies: +5 Vdc ± 15 Vdc +24 Vdc MCL +24 Vdc MCL +5 Vdc and ±...
Page 326: Voltage Verification
SERVICE AND REPAIR PROCEDURES POWER SUPPLY VOLTAGE VERIFICATION AND ADJUSTMENT Figure 4.20-1 Use a Long Screwdriver to Brace the Upper Cover Open Cytometer upper cover (open) Long screwdriver (with plastic handle) Channel (upper right) 7029129F Voltage Verification Locate J66 attached to the lower edge of the front of the Analyzer backplane. For the location of J66, refer to Figure A.2-2.
Page 327: Voltage Adjustment
SERVICE AND REPAIR PROCEDURES POWER SUPPLY VOLTAGE VERIFICATION AND ADJUSTMENT Figure 4.20-2 J66 and Pin Locations for +24 Vdc Voltage Verification 24 Vdc 13 or 14 10 or 9 7029216F Verify the DVM voltage reading matches the voltage reading recorded on the sticker attached to the side of the Data Acquisition card cage.
Page 328: Vdc Power Supply
SERVICE AND REPAIR PROCEDURES POWER SUPPLY VOLTAGE VERIFICATION AND ADJUSTMENT +5 Vdc Power Supply Preparation Remove the Cytometer top cover. Refer to Heading 4.3, COVER REMOVAL AND REINSTALLATION if you need detailed instructions. Lift the Data Acquisition card cage and lock it in its vertical position. Refer to Heading 4.3 if you need detailed instructions.
Page 329: +15 Vdc Voltage Verification
SERVICE AND REPAIR PROCEDURES POWER SUPPLY VOLTAGE VERIFICATION AND ADJUSTMENT +15 Vdc Voltage Verification At the Analyzer backplane, connect the negative DVM test lead to TP5 (AGND) and the positive DVM test lead to TP4 (orange). For test point and/or ground locations, refer to Figure A.2-3.
Page 330: Mcl Power Supply
SERVICE AND REPAIR PROCEDURES POWER SUPPLY VOLTAGE VERIFICATION AND ADJUSTMENT MCL Power Supply Preparation Remove the left-side cover. Refer to Heading 4.3, COVER REMOVAL AND REINSTALLATION if you need detailed instructions. Note: The MCL CPU card is the large card towards the rear of the Cytometer. Voltage Verification Table 4.20-1, locate the voltage you wish to check.
Page 331: Voltage Adjustment
SERVICE AND REPAIR PROCEDURES POWER SUPPLY VOLTAGE VERIFICATION AND ADJUSTMENT Voltage Adjustment ATTENTION: The MCL voltages are measured at the MCL CPU card, but adjustments are made on the corresponding MCL power supply in the right-side compartment of the Power Supply module.
Page 332 SERVICE AND REPAIR PROCEDURES POWER SUPPLY VOLTAGE VERIFICATION AND ADJUSTMENT 4.20-8 PN 4237029F...
Page 333: 4.21 Re-Imaging Flowcentre™ Computers
DOS and a 2 GB (Drive D) partition for Windows. You will not be able to use the full 6.4 GB of the drive because it would require that Beckman Coulter purchase a new license for using Windows 95 version in our computers. This is not going to be done.
Page 334: Re-Imaging A Flowcentre Desktop Computer With A Cache Controller Card
DOS and a 2 GB (Drive D) partition for Windows. You will not be able to use the full 6.4 GB of the drive because it would require that Beckman Coulter purchase a new license for using Windows 95 version in our computers. This is not going to be done.
Page 335 Re-load any Windows based software the customer purchased from Beckman Coulter. Re-boot into DOS and re-load any of the customers files needed.
Page 336 SERVICE AND REPAIR PROCEDURES RE-IMAGING FlowCentre™ COMPUTERS 4.21-4 PN 4237029F...
Page 337: 4.22 Universal Cd-Rom Driver Setup
SERVICE AND REPAIR PROCEDURES UNIVERSAL CD-ROM DRIVER SETUP 4.22 UNIVERSAL CD-ROM DRIVER SETUP Purpose This procedure provides the instructions for installing a driver that works with all types of CD-ROM drives. This driver can be setup on any computer using Windows 95 or higher. Tools/Supplies Needed B Unused 3.5 in.
Page 338 SERVICE AND REPAIR PROCEDURES UNIVERSAL CD-ROM DRIVER SETUP At the C:\> prompt (DOS prompt), type editßconfig.sys Add this line: DEVICE=C:\OAKCDROM.SYS/D:MSCD000 Save and close the file. At the DOS prompt (C:\), type editßautoexec.bat. In the autoexec.bat file: Make sure the line C:\DOS\MSCDEX.EXE/D:MSCD000 is displayed. If not, add the line, then save and close the file.
Page 339: 4.23 Bar-Code Scanner Eeprom Custom Programming
XL-MCL without check digits or wishing to change the recommended character lengths for bar-code labels must sign a letter from Beckman Coulter stating that the laboratory will take full responsibility for misreads, or mislabeling. Contact Technical Support for proper documentation to record this agreement.
Page 340: Computer Setup
SERVICE AND REPAIR PROCEDURES BAR-CODE SCANNER EEPROM CUSTOM PROGRAMMING This information is important later in this procedure. Figure 4.23-1 Bar-Code Decoder Card with Component Locations Ber-Code U13 (EEPROM) Decorder card port 7029171F Connect the female end of the bar-code communications cable to the AUX port on the Bar-Code Decoder card (Figure 4.23-1).
Page 341: Custom Programming Sequence
SERVICE AND REPAIR PROCEDURES BAR-CODE SCANNER EEPROM CUSTOM PROGRAMMING At the dialog box: Connect To Locate Connect using: Click on the down arrow to open the drop-down selection. Select COM 1 At the Port Settings tab inside the COM1 Properties dialog box: Select the communication protocol required for the EEPROM version (original or ALL CODES) installed on the Bar-Code Decoder card.
Page 342: If You Want To Store This Custom Configuration (Optional)
SERVICE AND REPAIR PROCEDURES BAR-CODE SCANNER EEPROM CUSTOM PROGRAMMING Locate Table F .2-1, MCL Bar-Code Scanner - Default Configuration. Use this information as a reference. Code 128 settings must reflect the Default Configuration. While monitoring the information to the right of the CODE TYPES ----> prompt, press ß...
Page 343: Reinstall Covers
SERVICE AND REPAIR PROCEDURES BAR-CODE SCANNER EEPROM CUSTOM PROGRAMMING When the message Do you want to save session? appears, select No. The Windows desktop reappears. Reinstall Covers Turn off the Cytometer. If SYSTEM II™ is your customer’ s preferred software, at the C:\XL> prompt, type XLOFF and press Û...
Page 344 SERVICE AND REPAIR PROCEDURES BAR-CODE SCANNER EEPROM CUSTOM PROGRAMMING 4.23-6 PN 4237029F...
Page 345 CONTENTS MAINTENANCE PROCEDURES, 5.1-1 SYSTEM VERIFICATION PROCEDURE (SVP), 5.1-1 Purpose, 5.1-1 Required Service Form, 5.1-1 Tools/Supplies Needed, 5.1-1 Preparation, 5.1-1 Static Tests, 5.1-1 Power Up Tests, 5.1-2 Trans Data Acquisition Card Offset Verification, 5.1-2 Acquisition, CV Analysis and Carryover Check, 5.1-2 Using the Manual Sample Station, 5.1-2 Using the MCL Sample...
Page 346 CONTENTS 5-ii PN 4237029F...
Page 347: Maintenance Procedures
MAINTENANCE PROCEDURES SYSTEM VERIFICATION PROCEDURE (SVP) Purpose Doing the System Verification Procedure (SVP) at the end of a service call ensures the instrument is working correctly before you leave. Fill in a copy of the SVP form and leave it with the customer.
Page 348: Power Up Tests
MAINTENANCE PROCEDURES SYSTEM VERIFICATION PROCEDURE (SVP) Inspect the segmenting valve for leakage and corrosion. Clean and repair as needed. Inspect the flow cell area for leakage and saline deposit build-up. Clean and repair as needed. Inspect the air/water filter separators (water traps). Clean and repair as needed: One in the Cytometer One in the Power Supply module.
Page 349 MAINTENANCE PROCEDURES SYSTEM VERIFICATION PROCEDURE (SVP) Remove the test tube from the manual sample station. Discard the test tube. Repeat steps through until all the test tubes labeled BEADS are processed. At the end of run 10, record the time it took to reach 5,000 events. 10.
Page 350: Using The Mcl Sample Station
MAINTENANCE PROCEDURES SYSTEM VERIFICATION PROCEDURE (SVP) Using the MCL Sample Station At the Workstation, select the Acquire Fluorospheres protocol. Dispense approximately 1 mL of Flow-Check fluorospheres into 10 test tubes and label each tube BEADS Dispense approximately 1 mL of sheath fluid into two test tubes and label each tube Place the test tubes labeled BEADS in the MCL carousel.
Page 351: Interlock Checks
MAINTENANCE PROCEDURES SYSTEM VERIFICATION PROCEDURE (SVP) Interlock Checks If the MCL option is installed, check the MCL door interlock: Place an empty test tube in the MCL carousel but leave the MCL door open. Attempt to run the Acquire Fluorospheres protocol again. If MCL operation halts immediately and an MCL Door Open Error or MCL Door Open Warning message appears on the Workstation screen, proper operation of the MCL interlock is confirmed.
Page 352: Workstation Computer Test
MAINTENANCE PROCEDURES SYSTEM VERIFICATION PROCEDURE (SVP) Workstation Computer Test Go to the DOS directory. Run SCANDISK C: and verify disk space available is adequate enough for the operator. On-Site Close Out Have the customer run one or more samples to verify operation. Ensure that all the customer’...
Page 353: Preventative Maintenance Inspection (Pmi)
MAINTENANCE PROCEDURES PREVENTATIVE MAINTENANCE INSPECTION (PMI) PREVENTATIVE MAINTENANCE INSPECTION (PMI) Purpose Use this procedure to perform a PMI on the instrument. Tools/Supplies Needed B XL PMI kit, PN 6913241 Procedure Check the contents of the XL PMI kit against the packing list to ensure that all the parts are present.
Page 354: System Verification
MAINTENANCE PROCEDURES PREVENTATIVE MAINTENANCE INSPECTION (PMI) 10. At the MS-DOS prompt: If running under MS-DOS 5.0, type CHKDSK/F and press Û. If running under MS-DOS 6.0 or 6.22, type SCANDISK and press Û. After this is completed, type DEFRAG and press Û. 11.
Page 355 COULTER CYTOMETRY SYSTEMS VERIFICATION FORM Institution Name Date FSR # Model / Serial # Run # PROBLEM / SYMPTOM CORRECTIVE ACTION SMOs PERFORMED On all types of maintenance completed, VERIFY: HPCV Precision for FS & FL Linear Parameters on Flourospheres * HPCV Stability on Flourospheres * Peak or Mean Channel Stability for All Linear Parameters* That All Customer Concerns Have Been Addressed (Verified by customer signature.)
Page 356 Checked Box Indicates Function Performed PROCEDURE / VERIFICATION PERFORMANCE - ON ALL CALL COMPLETIONS COMPUTER / WORKSTATION HPCV Precision on FS & FL Linear Parameters CMOS Setup Verified HPCV Stability on FS & FL Linear Parameters Verified Config.sys / Autoexec.bat Peak or Mean Channel Stability Checked for Viruses Carryover...
Page 357 CONTENTS SCHEMATICS, 6.1-1 ENGINEERING SCHEMATICS, 6.1-1 PN 4237029F...
Page 358 CONTENTS 6-ii PN 4237029F...
Page 359: Schematics
SCHEMATICS ENGINEERING SCHEMATICS The following is a list of the engineering schematics you need for troubleshooting an XL or XL-MCL flow cytometer. Name Amplifier / Signal Conditioner card 6320739 Analyzer Backplane 6320633 CYT12 Receiver EMC 6322948 Cyto Transputer card 6320738 FALS Hybrid Detector card 6321049 Fiber Optic Interface card...
Page 360 SCHEMATICS ENGINEERING SCHEMATICS Electronic (.pdf) files of these schematics are available on a separate CD-ROM in the Service ® Resource Kit (SRK) and in a Lotus Notes database. The schematics in the SRK are the latest revisions available at the time the SRK is released. For copies of schematics released between revisions of the SRK, check the Lotus Notes database.
Page 361 CONTENTS TROUBLESHOOTING, 7.1-1 LEVEL SENSE INDICATORS AND ERROR MESSAGES, 7.1-1 Level Sense Indicators, 7.1-1 Cleanse Low, 7.1-1 Sheath Low and Waste Full, 7.1-1 Error Messages, 7.1-1 About the Error Messages Table, 7.1-2 PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL, 7.2-1 Purpose, 7.2-1 Tools/Supplies Needed, 7.2-1...
Page 362 CONTENTS Grand Canyon Test, 7.2-6 Purpose, 7.2-6 Circuit Cards Checked in this Test, 7.2-6 Response if Test Fails, 7.2-6 Histogram Test, 7.2-7 Purpose, 7.2-7 Initialize System, 7.2-7 Purpose, 7.2-7 Circuit Cards Checked in this Test, 7.2-7 Response if Test Fails, 7.2-7 Laser Control, 7.2-8 Purpose, 7.2-8...
Page 363 CONTENTS MCL POWER UP Status, 7.2-13 Purpose, 7.2-13 Response if Test Fails, 7.2-13 MCL Probe Align, 7.2-13 Purpose, 7.2-13 MCL ROM Test, 7.2-13 Purpose, 7.2-13 Response if Test Fails, 7.2-13 MCL Scan Reliability, 7.2-13 Purpose, 7.2-13 MCL Stepper Noise Test, 7.2-13 Purpose, 7.2-13 Response if Test Fails, 7.2-13...
Page 364 CONTENTS Pulse RAMP Test, 7.2-22 Purpose, 7.2-22 Components Checked in this Test, 7.2-22 Response if Test Fails, 7.2-22 Test, 7.2-22 Purpose, 7.2-22 Circuit Card Checked in this Test, 7.2-22 Response if Test Fails, 7.2-22 Beads, 7.2-22 Purpose, 7.2-22 Sample Leak Test, 7.2-23 Purpose, 7.2-23 Components Checked in this...
Page 365 CONTENTS XY Display - Not Used, 7.2-27 TABLES 7.1-1 Level Sense Indicators - Sheath Low and Waste Full, 7.1-1 7.1-2 Error Messages, 7.1-2 PN 4237029F...
Page 366 CONTENTS 7-vi PN 4237029F...
Page 367: Troubleshooting
TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES Level Sense Indicators Cleanse Low When the CLEANSE LOW indicator is red, fill the cleanse container. You cannot start a cleanse cycle until this is done. Sheath Low and Waste Full Table 7.1-1 documents how to read the SHEATH LOW and WASTE FULL level-sense indicators.
Page 368: About The Error Messages Table
TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES About the Error Messages Table The table of error messages (Table 7.1-2) lists the error messages in alphabetical order, with their cause and what to do about them. An additional error message table is printed automatically when you install SYSTEM II software.
Page 369 TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES Table 7.1-2 Error Messages (Continued) Message Cause/Symptom Action Equation(s) exceed defined Software failure. At Computer Workstation, turn instrument limitations off, then back on. ERROR: 2 parameter histograms Two-parameter histogram selected as Select one-parameter histogram. not allowed control or test in Immuno-4 data analysis.
Page 370 TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES Table 7.1-2 Error Messages (Continued) Message Cause/Symptom Action ERROR! -- Unable to read non-XL Attempted to create protocols using Use valid protocol. protocols! non-XL/XL-MCL flow cytometer software. >>>> Error loading cytometer Software unable to program Reset SYSTEM II software program.
Page 371 TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES Table 7.1-2 Error Messages (Continued) Message Cause/Symptom Action Host command invalid Unknown command. At Computer Workstation, turn instrument off, then back on. Illegal Cytometer state Cytometer error. Insufficient memory available System needs more memory. Insufficient memory for listmode System needs more memory to replay replay...
Page 372 TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES Table 7.1-2 Error Messages (Continued) Message Cause/Symptom Action MCL Error MCL option disconnected. r Reset circuit breakers (see Special Procedures and Troubleshooting manual). r Check blue, flat-ribbon cable. r Check MCL CPU card. MCL Probe Up/Down Error Probe did not go down or stuck in Press AUTO.
Page 373 TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES Table 7.1-2 Error Messages (Continued) Message Cause/Symptom Action No valid histogram files were r Specified wrong path Specify correct directories on Utilities found Configuration screen and/or from File r No directory files. menu, select Rebuild. No valid listmode files were found No valid protocol files were found Parameters are not present...
Page 374 TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES Table 7.1-2 Error Messages (Continued) Message Cause/Symptom Action Selected signal must be a Selected signal that is not a parameter First select signal as parameter. parameter to numerator/denominator for RATIO parameter. Selection, edit or erase of ratio not Attempted selecting, editing, or None.
Page 375 TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES Table 7.1-2 Error Messages (Continued) Message Cause/Symptom Action System vacuum error r Vacuum low Verify: System vacuum warning r VAC TRAP loose or full 1. VAC TRAP (on front of Power Supply module) secure with <0.5 in. fluid. If it r Bad vacuum hose.
Page 376 TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES Table 7.1-2 Error Messages (Continued) Message Cause/Symptom Action r You pressed È or Ê during Unable to find the panel info Select panel again and/or specify correct panel selection directories on Utilities Configuration screen.
Page 377 TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES Table 7.1-2 Error Messages (Continued) Message Cause/Symptom Action Waste backpressure err Air filter or its vent tubing clogged Bypass clogged air filter (front of Power Supply module) or vent tubing (above filter) by disconnecting connector underneath FILTER.
Page 378 TROUBLESHOOTING LEVEL SENSE INDICATORS AND ERROR MESSAGES 7.1-12 PN 4237029F...
Page 379: Prefinal Service Software - A Troubleshooting Tool
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Purpose Prefinal Service software can provide a valuable information for locating a problem. this software must be installed and operated using the guidelines provided under Heading 4.2, PREFINAL SERVICE SOFTWARE INSTALLATION AND OPERATION.
Page 380: Circuit Card Checked In This Test
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Circuit Card Checked in this Test Trans Data Acquisition card Response if Test Fails Jumper E2 to E3 may not be properly installed. Trans Data Acquisition card may be defective. Amp Gain Control Purpose This selection controls the Amp/Signal Conditioner gain for all the signals.
Page 381: Attenuator Control
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Attenuator Control Purpose This selection controls the Amp/Signal Conditioner card attenuator for all signals. At the Workstation keyboard, select the desired parameter (PMT1, PMT2, PMT3, PMT4, AUX, FS, or SS). This selection does not provide a PASS or FAIL message. Use the æ or ç key to select the parameter.
Page 382: Canyon Jumper Test
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Canyon Jumper Test Purpose This test ensures that the RAMP jumper (E4 to E5) is installed on each Amp/Signal Conditioner card. If the jumper is properly installed on each card, PASS is displayed. If a jumper is not properly installed on a Amp/Signal Conditioner card, the screen freezes with the name of the parameter displayed in red.
Page 383: Circuit Cards Checked In This Test
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Circuit Cards Checked in this Test Top Panel Display 2 card Front Panel LED and Switch Input 2 card (in the sample station of an XL-MCL Cytometer) Front Panel LED and Switch Input card (in the sample station of an XL without the MCL option) Interconnecting cable from the Analyzer backplane to the Front Panel LED and Switch Input 2 card or Front Panel LED and Switch Input card, as applicable...
Page 384: Grand Canyon Adjust
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Grand Canyon Adjust ATTENTION: The Grand Canyon Adjust must be performed anytime the Trans Data Acquisition card or an Amp/Signal Conditioner card is replaced. Always run the ADC Zero Adjust before doing this adjustment. Before starting is adjustment, remove jumper E4 to E5 from any Amp/Signal Conditioner card being tested.
Page 385: Histogram Test
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Histogram Test Purpose This test checks the histograms generated by the ADC on the Trans Data Acquisition card. It checks for gaps between channels of a normal distribution. To select the parameter to test, press è or é, as applicable. To select the number of bits used to generate the histogram, press æ...
Page 386: Laser Control
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Laser Control Purpose This selection controls the laser. The laser can be turned ON and OFF through this screen. The power and current from the laser are monitored and displayed on the screen. Options At the Workstation keyboard, select the desired option using the æ...
Page 387: Laser Warm Up
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Laser Warm Up Purpose This selection shows the warm-up characteristics of the laser. It plots the laser current versus time on the Workstation screen. Lock Up Test - Not Used MCL Bar Code Head Test WARNING The laser beam can cause eye damage if viewed either directly or indirectly from reflective surfaces (such as a mirror or shiny metal surface).
Page 388: Mcl Burn In
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL MCL Burn In Purpose This selection cycles the MCL through a carousel continuously. A carousel full of sample tubes with bar-code labels is used to perform this test. All errors are reported on the Workstation screen.
Page 389: Mcl Carousel Status
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL MCL Carousel Status Purpose This selection ensures that the MCL Carousel Tube Position and Home sensors are working properly. Test Sequence The carousel is stepped until the Home sensor goes inactive then active. If this does not take place within a full revolution, an error is reported.
Page 390: Mcl Manual Control
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL MCL Manual Control Purpose This selection allow manual control over the MCL movements. The MCL sensor status is also reported. Sensor Status A dash (—) indicates the sensor is inactive. An asterisk (*) indicates the sensor is activated. Controls To select what moves, press the æ...
Page 391: Mcl Power Up Status
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL MCL POWER UP Status Purpose This selection causes the MCL to do a system reset which causes it to go through its normal power up reset diagnostics. This is the same command the Cytometer software uses to power up the MCL.
Page 392: Mcl Terminal
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL MCL Terminal Purpose This selection allow <ESC> codes to be sent to the MCL through the Cytometer parallel port. The MCL can then be controlled manually using its most primitive commands. Commands Perform a system reset before running any of these commands.
Page 393 TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Commands Keystrokes to Initiate ÈDL Door Commands DOOR_LOCK_CMD Note: Perform a system reset before ÈDO DOOR_WAS_OPENED_CMD running any of these commands. ÈDU DOOR_UNLOCK_CMD MCL_RESET_CMD (ÈMR) MCL_VER_LEVEL_CMD (ÈMV) Any attempt to run a command without first resetting the system will be ignored.
Page 394 TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Commands Keystrokes to Initiate ÈMS Status Commands MCL_STATUS_CMD Note: Any status command may be ÈCS CARO_STATUS_CMD performed without first performing a ÈDS DOOR_STATUS_CMD system reset. Use these commands ÈHS HEAD_STATUS_CMD with caution! ÈLS RLV_STATUS_CMD ÈPS...
Page 395: Memory Test
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Memory Test Purpose This test ensures that the external memory in all the transputers is working properly. An XL or XL-MCL instrument may have either three or four transputers. These transputers are located on the Opto Transprocessor EXMEM or Opto Transprocessor EXMEM II in the Workstation computer and on the Cyto Transputer card in the Cytometer.
Page 396: Response If Test Fails
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Cyto Transputer card contains two transputers, designated T3 and T4. Transputer T3 is located in socket U52. Its memory is made up of eight 1 MB x 1 Bit DRAMs (U31, U37, U43, U47, U50, U55, U58, and U59). Transputer T4 is located in socket U54.
Page 397: Response If Test Fails
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL OPTO DMA Test Purpose This test ensures that the Opto Transprocessor EXMEM card or Opto Transprocessor EXMEM II card can perform a Direct Memory Access (DMA) operation to the Workstation computer. The test transfers 256 bytes in each direction and verifies the data. it also ensures that DMA channel 0 (jumpers X7 and X8) is selected.
Page 398: Response If Test Fails
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL OPTO Link Test Purpose This test ensures that the Transputer Link path from the Opto Transprocessor EXMEM card or Opto Transprocessor EXMEM II card in the Workstation computer through the optical cable and into the last (third or fourth) transputer located on the Cyto Transputer card in the Data Acquisition card cage in the Cytometer is working properly.
Page 399: Response If Test Fails
TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL PMT Voltage Control Purpose This selection controls the high voltage supplies for PMT1, PMT2, PMT3, and PMT4. A separate Bertan power supply drives each Amp/Signal Conditioner card. Note: PMT4 and the Bertan power supply for PMT4 are on 4-color systems only. At the Workstation keyboard, select the desired PMT using the æ...
Page 400 TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Response if Test Fails System Interface card may be defective. Sensor may be defective. Pulse RAMP Test Purpose This test ensures the bar graphs on the front panel display are working properly. These signals originate from the Trans Data Acquisition card.
Page 401 TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Sample Leak Test Purpose This test ensures that the sample station seals on the manual sample station and the MCL sample station are not leaking. To perform this test, a sample tube must be inserted in the manual sample station and a fully loaded carousel must be loaded in the MCL.
Page 402 TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL T805 Test Purpose This test ensures that the T805 chip is in the proper socket on the Opto Transprocessor EXMEM card (non-EMC version used in XL and XL-MCL instruments with the serial number Z09062 and below) or Opto Transprocessor EXMEM II card (EMC version used in all XL and XL-MCL instruments with the serial number Z09063 and above), as applicable.
Page 403 TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Valve Control Purpose This selection controls the pneumatic system. It allows manual control over each individual valve in the system. The sheath and sample pressures are also monitored and displayed here. The Sheath Sample Differential can also be adjusted here. If an emergency shutdown is required, pressing the ß...
Page 404 TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Valve Sequence Purpose This selection allow the system to be drained and cleaned out for shipment. DRAIN VACUUM CHAMBER Drains the waste chamber if full. SHUTDOWN AND CLEANUP Performs the shutdown and cleanup procedure on the Cytometer to ready it for shipment. VME Addr Bus Test Purpose This test ensures that the Cyto Transputer card can communicate with each card in the Data...
Page 405 TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL Circuit Cards Checked in this Test Cyto Transputer card System Interface card Trans Data Acquisition card Amp/Signal Conditioner card for PMT1, PMT2, PMT3, PMT4, AUX, FS, and SS Response if Test Fails VME Data Bus may be open or shorted.
Page 406 TROUBLESHOOTING PREFINAL SERVICE SOFTWARE - A TROUBLESHOOTING TOOL 7.2-28 PN 4237029F...
Page 407 CONTENTS PARTS LISTS, 8.1-1 MASTER PARTS LISTS, 8.1-1 ILLUSTRATED PARTS, 8.2-1 Overview, 8.2-1 Locating a Part Number, 8.2-1 ILLUSTRATIONS 8.2-1 XL Cytometer with MCL Option, Illustrated Components, 8.2-2 8.2-2 XL-MCL Cytometer Covers, Doors, and Latches (See Table 8.2-2), 8.2-4 8.2-3 MCL Covers and Associated Hardware (See Table 8.2-3), 8.2-6 8.2-4...
Page 408 CONTENTS 8.2-37 Lower Pneumatics Drawer (See Table 8.2-37), 8.2-62 8.2-38 Pinch Valve and Associated Components, Lower Pneumatics Drawer (See Table 8.2-38), 8.2-64 8.2-39 Solenoid Manifold, Lower Pneumatics Drawer (See Table 8.2-39), 8.2-66 8.2-40 QD10 and QD11 (Coupling and Fittings), Lower Pneumatics Drawer (See Table 8.2-40), 8.2-67 8.2-41 Quick-Disconnect Fittings and Brackets, Lower Pneumatics Drawer...
Page 409 CONTENTS 8.1-16 Master Parts List - V, 8.1-32 8.1-17 Master Parts List - W, 8.1-33 8.2-1 Illustrations Not Referenced from Figure 8.2-1, 8.2-3 8.2-2 XL-MCL Cytometer Covers, Doors, and Latches (See Figure 8.2-2), 8.2-5 8.2-3 MCL Covers and Associated Hardware (See Figure 8.2-3), 8.2-7 8.2-4 XL Cytometer Covers, Doors, and Latches (See Figure...
Page 410 CONTENTS 8.2-41 Quick-Disconnect Fittings and Brackets, Lower Pneumatics Drawer (See Figure 8.2-41), 8.2-69 8.2-42 Cytometer Rear Panel, Lower (See Figure 8.2-42), 8.2-71 8.2-43 Fan Assembly, Cytometer Rear Panel (See Figure 8.2-43), 8.2-73 8.2-44 Cytometer Right Side Compartment (See Figure 8.2-44), 8.2-75 8.2-45 Power Supply Module - Left Side with Cover Removed (See Figure 8.2-45), 8.2-77...
Page 411 PARTS LISTS MASTER PARTS LISTS ATTENTION: Part numbers issued by the Oracle system are six-digit numbers. The components in the master parts list are listed alphabetically by their common names or categories. Table 8.1-1 lists the common names and categories used, further defines the categories, and references the table in this section where those components appear.
Page 412 8.1-14 Tank Sheath, cleanse, waste (also referred to as container or bottle) 8.1-15 Target 8.1-15 Tie wrap 8.1-15 Tool Individual hand tools, Beckman Coulter service tool kit, Service Resource Kit 8.1-15 Transducer 8.1-15 Tray 8.1-15 Tubing PEEK, polyurethane, silicone 8.1-15...
Page 413: Back
PARTS LISTS MASTER PARTS LISTS Table 8.1-2 Master Parts List - A and B Description Part Number Figure Item Adaptor, cleaning tube 6859297 Arm. probe, for manual sample station 1020873 8.2-33 Assembly, front door, Power Supply module, black 6856160 8.2-5 Assembly, front door, Power Supply module, grey 6807047 8.2-5...
Page 414 PARTS LISTS MASTER PARTS LISTS Table 8.1-3 Master Parts List - C (Continued) Description Part Number Figure Item Cable, +24 Vdc, EMC 6028689 Cable, +5 Vdc 6028305 Cable, +5 Vdc, EMC 6028687 ± 6028306 Cable, 15 Vdc ± 6028688 Cable, 15 Vdc, EMC Cable, analog/logic power (non EMI) 6028307...
Page 415 PARTS LISTS MASTER PARTS LISTS Table 8.1-3 Master Parts List - C (Continued) Description Part Number Figure Item 6028431 Cable, MCL, dc, CYTO 6028699 Cable, MCL, dc, CYTO, EMC Cable, scanner interface 6028432 Cable, sensor 6028290 Cable, sensor/reg control (FL rib) 6028289 Cable, sensor/reg control, shielded, EMC 6028702...
Page 416 PARTS LISTS MASTER PARTS LISTS Table 8.1-3 Master Parts List - C (Continued) Description Part Number Figure Item Choke, plastic, brown, 0.025 in. orifice 6213015 8.2-59 Note: Designated as CK 4, 5, 6, 7, 8, 9, 10, and 11 on page 1 of the XL System Pneumatic / Hydraulic Layout, PN 6320886.
Page 417 PARTS LISTS MASTER PARTS LISTS Table 8.1-3 Master Parts List - C (Continued) Description Part Number Figure Item Computer, Pentium III processor, 550 MHz minimum, referred to as FlowCentre II 2016874 8.2-6 tower computer, also includes PN 2016876 (mouse) and PN 2016881 (keyboard) Computer, Pentium processor, 166 MHz minimum, 16 MB RAM, minimum 1.2 GB 2016802(R) HDD, minimum 2 MB video, Atlas PCI III, replacement for PN 2016669, also...
Page 418 PARTS LISTS MASTER PARTS LISTS Table 8.1-3 Master Parts List - C (Continued) Description Part Number Figure Item Cover, left-side, for XL-MCL Cytometer, black 6858522 8.2-2 Cover, left-side, for XL-MCL Cytometer, grey 6807076 8.2-2 Cover, Power Supply module three-surface (left-side, top, right-side), black 6856369 8.2-5 Cover, Power Supply module three-surface (left-side, top, right-side), grey...
Page 419 PARTS LISTS MASTER PARTS LISTS Table 8.1-4 Master Parts List - D Description Part Number Figure Item Data entry, keyboard, long 2016392 Data entry, keyboard, short 2016592 Data entry, membrane, overlay and switch, for XL-MCL sample station 1021734 8.2-30 Data entry, overlay and membrane switch sample cup XL 1016815 8.2-32 Note: Before installation, remove paper backing.
Page 420 PARTS LISTS MASTER PARTS LISTS Table 8.1-4 Master Parts List - D (Continued) Description Part Number Figure Item Door, front panel display, black 6856490 8.2-2 Note: Attach door using two 3-in. #5 swag hinges, PN 1021176. 8.2-4 Attach each hinge using four sets of self-lock screws (#6-32 x 0.37-in. length, 8.2-12 pan-head), PN 2839039, and #6 flat washers, PN 2827147.
Page 421 PARTS LISTS MASTER PARTS LISTS Table 8.1-5 Master Parts List - F (Continued) Description Part Number Figure Item Filter, gas, hydrophobic, 0.2 micron, disposable plastic 6232561 8.2-47 8.2-53 Filter, light, used in filter slot 1, 525 band pass (525 BP) 3814134 8.2-20 Filter, light, used in filter slot 2, 575 band pass (575 BP)
Page 422 PARTS LISTS MASTER PARTS LISTS Table 8.1-5 Master Parts List - F (Continued) Description Part Number Figure Item Fitting, hose barb union, 0.062 i.d. to 10-32 threaded 6232086 8.2-15 8.2-17 8.2-33 8.2-35 8.2-36 8.2-39 8.2-44 Fitting, hose barb union, 0125 i.d. to 10-32 threaded 6232085 8.2-17 8.2-44...
Page 423 PARTS LISTS MASTER PARTS LISTS Table 8.1-5 Master Parts List - F (Continued) Description Part Number Figure Item Fitting, insert, white male quick-connect, internal connector to 0.082 i.d. hose barb 6232581 8.2-18 8.2-40 8.2-41 Fitting, Luer, 0.125 i.d. 6232527 Fitting, miniature adapter, 10-32 tap in 0.125 MPT plug, nickel-plated brass 6232683 8.2-35 Fitting, miniature tee branch with 10-32 ports...
Page 424 PARTS LISTS MASTER PARTS LISTS Table 8.1-5 Master Parts List - F (Continued) Description Part Number Figure Item Fitting, quick-connect, panel mount, blue female body with nut, automatic shut-off, 6232309 8.2-42 0.125 flow x 0.250 o.d. (couples with blue insert, PN 6232306, also with automatic 8.2-57 shut-off) Note: If installing the quick-connect on the Cytometer rear panel, insert a #50...
Page 425 PARTS LISTS MASTER PARTS LISTS Table 8.1-5 Master Parts List - F (Continued) Description Part Number Figure Item Flow cell 6858386 8.2-23 Flow cell, complete 6859156 Foot, rubber 6858988 8.2-2 Note: Use setscrew, PN 2810028, to attach foot to the Cytometer frame. 8.2-4 Frame, Power Supply module 6856206...
Page 426 PARTS LISTS MASTER PARTS LISTS Table 8.1-5 Master Parts List - F (Continued) Description Part Number Figure Item FRU, MCL carousel-home sensor cable assembly 7000435 FRU, MCL head sensor cable assembly 7000438 8.2-9 FRU, MCL lift motor (up/down) sensor cable assembly 7000437 FRU, MCL lifter assembly 7000443...
Page 427 PARTS LISTS MASTER PARTS LISTS Table 8.1-5 Master Parts List - F (Continued) Description Part Number Figure Item FRU, sample station, manual, for XL-MCL Cytometer with black covers 7000354 8.2-2 8.2-30 Front view 8.2-31 Rear view FRU, segmenting valve assembly 7000370 8.2-34 FRU, segmenting valve front pad...
Page 428 PARTS LISTS MASTER PARTS LISTS Table 8.1-6 Master Parts List - G (Continued) Description Part Number Figure Item Gauge, 0 to 30 in. Hg, for monitoring system vacuum, 1.5-inch diameter, panel 6232183 8.2-47 mount (includes panel mounting hardware) 8.2-54 Note: Apply a thin line of pipe sealant, PN 1601056, across gauge threads before attaching the fitting.
Page 429 Kit, re-image, for the FlowCentre II tower computer, PN 2016874 7270464 Kit, Sample/MCL Intro Line 6912941 8.2-34 Kit, sheath filter replacement 6912942 8.2-26 Kit, software replacement, COULTER EPICS XL/XL-MCL SYSTEM II Version 3.0 6915303 Knob, pinch valve 1018728 8.2-27 Knob, snout 1021682 8.2-23 8.1-19...
Page 430 PARTS LISTS MASTER PARTS LISTS Table 8.1-9 Master Parts List - L Description Part Number Figure Item Label (J69) 2427902 8.2-44 Label, 488 BK / Slot 5 2429706 8.2-20 Label, 488 DL / Slot 4 2429704 8.2-20 Label, 525 BP / Slot 1 2429708 8.2-20 Label, 550 DL / Slot 6...
Page 431 PARTS LISTS MASTER PARTS LISTS Table 8.1-10 Master Parts List - M Description Part Number Figure Item Magnet, disk, rare earth, 0.187 o.d. x 0.063 thickness 2523394 8.2-32 Note: Before installation, remove the sample station door and lay the door near the edge of a table with the handle positioned over the edge.
Page 432 PARTS LISTS MASTER PARTS LISTS Table 8.1-10 Master Parts List - M (Continued) Description Part Number Figure Item Mouse pointing device, for use with 166 MHz Pentium computer PN 2016802 and 2016161 133 MHz Pentium computer PN 2016669 Mouse pointing device, PS/2 compatible, for FlowCentre computer PN 2016753 2016665 8.2-7 Mouse pointing device, PS/2 scroll point, for FlowCentre II tower computer...
Page 433 PARTS LISTS MASTER PARTS LISTS Table 8.1-11 Master Parts List - N and O (Continued) Description Part Number Figure Item O-ring, ethylene propylene seal, 0.187 i.d. x 0.050 wall 2523062 8.2-15 8.2-17 8.2-27 8.2-33 8.2-35 8.2-36 8.2-39 8.2-44 O-ring, silicone seal, 0.364 i.d. x 0.070 width 2512031 8.2-29 8.2-37...
Page 434 PARTS LISTS MASTER PARTS LISTS Table 8.1-12 Master Parts List - P (Continued) Description Part Number Figure Item PCB, MCL CPU 6705712 8.2-8 PCB, MCL Interface 6705700 8.2-13 PCB, Motor Filter, EMC 6706409 8.2-8 PCB, Network Interface 2016621 PCB, Sensor 6705217 8.2-14 Note: Place a hole spacer (0.140 i.d.
Page 435 PARTS LISTS MASTER PARTS LISTS Table 8.1-12 Master Parts List - P (Continued) Description Part Number Figure Item PCB, PMT Distribution and Laser Fan Control 6705199 8.2-19 Note: Refers to the two cooling fans mounted on the Cytometer frame. These fans 8.2-24 cool the Argon laser.
Page 436 PARTS LISTS MASTER PARTS LISTS Table 8.1-12 Master Parts List - P (Continued) Description Part Number Figure Item Probe, sample, used in manual sample station 6858174 8.2-33 Promise, Ultra 66 IDE PCI Controller with cable, for FlowCentre tower computer 2016960 PN 2016753 Push-button, for opening MCL upper base door, black 6858700...
Page 437 PARTS LISTS MASTER PARTS LISTS Table 8.1-14 Master Parts List - S (Continued) Description Part Number Figure Item Sample station, long screw, top 2806117 Screw, machine (#2-56 x 0.19 in. length, pan-head) 2802006 8.2-3 Screw, machine (#4-40 x 0.25 in. length, HSC-head) 2804083 8.2-8 Screw, machine (#4-40 x 0.38 in.
Page 438 PARTS LISTS MASTER PARTS LISTS Table 8.1-14 Master Parts List - S (Continued) Description Part Number Figure Item Screw, machine, knurled (#4-40 x 0.75 in. length, hex socket head) 2851975 8.2-8 8.2-23 Screw, self-lock (#10-32 x 0.75 in. length, pan-head) 2839051 8.2-24 Screw, self-lock (#4-40 x 0.25 in.
Page 439 Socket joint, quick-disconnect with spring loaded sleeve, for 0.253-inch diameter 2523798 8.2-35 ball stud, stainless steel Software, COULTER EPICS XL/XL-MCL Prefinal, on 3.5 in. floppy diskette 7231244 Software, COULTER EPICS XL/XL-MCL SYSTEM II, Version 3.0 6706441 Software, EXPO32™ ADC Cytometer, on CD-ROM, also includes appropriate...
Page 440 PARTS LISTS MASTER PARTS LISTS Table 8.1-14 Master Parts List - S (Continued) Description Part Number Figure Item Spacer, internally and externally threaded (#6-32 x 0.250 DP to #6-32 x 0.375, 2851866 8.2-24 aluminum alloy) Spacer, tapped, 6-32 x 1.625 in. length x 0.250 in. hex, aluminum 2851363 8.2-37 Spring, extension, 0.180 o.d.
Page 441 8.2-61 Tie wrap, screw mount, #6 sizer, 7.4 in. long, 0.19 in wide 6011006 8.2-24 8.2-43 8.2-58 8.2-61 Tool kit, Beckman Coulter service 5415102 Tool, card extender 6705582 Tool, circuit analyzer, 3-wire 2906883 Tool, ESD kit, static-dissipative field kit 5415097...
Page 442 PARTS LISTS MASTER PARTS LISTS Table 8.1-15 Master Parts List - T (Continued) Description Part Number Figure Item Tubing, clear, polyurethane, 0.076 i.d. x 0.144 o.d., referred to as sheath tubing 3213215 Tubing, clear, polyurethane, 0.082 i.d. x 0.144 o.d. 3202036 Tubing, PEEK, flow cell intro line, 3.75 in.
Page 443 PARTS LISTS MASTER PARTS LISTS Table 8.1-16 Master Parts List - V (Continued) Description Part Number Figure Item Valve, pull-apart pinch, double-action, white, standard 6855763 8.2-15 8.2-17 8.2-27 8.2-37 8.2-38 Valve, single solenoid, pilot-actuated two-position five-port spool valve, 24 Vdc, 6232376 8.2-39 operating pressure 20 to 100 psi...
Page 444 PARTS LISTS MASTER PARTS LISTS Table 8.1-17 Master Parts List - W (Continued) Description Part Number Figure Item Washer, flat, #6 (0.156 i.d. x 0.375 o.d. x 0.031 in. thickness) 2827056 8.2-3 Washer, flat, #6 (0.156 i.d. x 0.375 o.d. x 0.046 thickness) 2827134 8.2-19 8.2-24...
Page 445 PARTS LISTS ILLUSTRATED PARTS ILLUSTRATED PARTS ATTENTION: Part numbers issued by the Oracle system are six-digit numbers. Overview To expedite finding a part number, the illustrations in this section are organized in functional groups. Figure 8.2-1 is the anchor illustration from which you can quickly access a specific illustration.
Page 446 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-1 XL Cytometer with MCL Option, Illustrated Components Figure Reference A Workstation r FlowCentre II, Figure 8.2-6 r FlowCentre, Figure 8.2-7 At the Cytometer . . . B MCL option, Figure 8.2-8 Vertical plate components, Figure 8.2-9 Coulter EPICS XL MCL...
Page 447 PARTS LISTS ILLUSTRATED PARTS Table 8.2-1 Illustrations Not Referenced from Figure 8.2-1 Title Figure Number XL-MCL Cytometer Covers, Doors, and Latches Figure 8.2-2 MCL Covers and Associated Hardware Figure 8.2-3 XL Cytometer Covers, Doors, and Latches Figure 8.2-4 Power Supply Cover, Door, Latches, and Casters Figure 8.2-5 Check Valves and Chokes with Related Components Figure 8.2-59...
Page 448 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-2 XL-MCL Cytometer Covers, Doors, and Latches (See Table 8.2-2) Rear view Coulter Epics XL • MCL 7029010F 8.2-4 PN 4237029F...
Page 449 PARTS LISTS ILLUSTRATED PARTS Table 8.2-2 XL-MCL Cytometer Covers, Doors, and Latches (See Figure 8.2-2) Item Part Number Description 6807080 Cover, top, grey 6858129 Cover, top, black Note: Attach using four sets of self-lock screws (#6-32 x 0.37-in. length, pan-head), PN 2839039, and #6 flat washers, PN 2827147. 6807079 Cover, right-side, grey 6856494...
Page 450 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-3 MCL Covers and Associated Hardware (See Table 8.2-3) 6 7 8 7029166F 8.2-6 PN 4237029F...
Page 451 PARTS LISTS ILLUSTRATED PARTS Table 8.2-3 MCL Covers and Associated Hardware (See Figure 8.2-3) Item Part Number Description 1021735 Tray, MCL drip 6807078 MCL upper base, grey 6858519 MCL upper base, black 1021737 MCL upper window Note: Before installing the window in the upper cover, remove the paper covering from the bottom side only (leave the paper on the top side), apply a thin line of epoxy, PN 1615132, to the rim of the upper cover, install the window, allow the epoxy to set, then remove the paper covering...
Page 452 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-4 XL Cytometer Covers, Doors, and Latches (See Table 8.2-4) Rear view Coulter Epics XL 7029173F 8.2-8 PN 4237029F...
Page 453 PARTS LISTS ILLUSTRATED PARTS Table 8.2-4 XL Cytometer Covers, Doors, and Latches (See Figure 8.2-4) Item Part Number Description 6807080 Cover, top, grey 6858129 Cover, top, black Note: Attach using four sets of self-lock screws (#6-32 x 0.37-in. length, pan-head), PN 2839039, and #6 flat washers, PN 2827147. 6807079 Cover, right-side, grey 6856494...
Page 454 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-5 Power Supply Cover, Door, Latches, and Casters (See Table 8.2-5) FILTER WATER TRAP TRAP SYSTEM POPWER FILTER MCL POPWER PRESS PRESS 7029131F 8.2-10 PN 4237029F...
Page 455 PARTS LISTS ILLUSTRATED PARTS Table 8.2-5 Power Supply Cover, Door, Latches, and Casters (See Figure 8.2-5) Item Part Number Description 2806017 Screw, machine (#6-32 x 0.38 in. length, pan-head) Note: Apply one drop of adhesive sealant, PN 1601065, to screw threads before insertion.
Page 456 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-6 FlowCentre II Workstation (See Table 8.2-6) 7029111F Rear view 8.2-12 PN 4237029F...
Page 457 PARTS LISTS ILLUSTRATED PARTS Table 8.2-6 FlowCentre II Workstation (See Figure 8.2-6) Item Part Number Description 2016972 Drive, diskette, 3.5 in., 1.44 MB, white 175742 Drive, CD-ROM, 8X minimum, internal IDE 2016874 Computer, Pentium III processor, 550 MHz minimum, referred to as FlowCentre II Tower Computer, also includes PN 2016876 (mouse) and PN 2016881 (keyboard) 2016938...
Page 458 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-7 FlowCentre Workstation (See Table 8.2-7) Rear view 7029221F 8.2-14 PN 4237029F...
Page 459 PARTS LISTS ILLUSTRATED PARTS Table 8.2-7 FlowCentre Workstation (See Figure 8.2-7) Item Part Number Description 2016753 Computer, Atlas PCI III Pentium 200 processor, referred to as FlowCentre Computer, also includes PN 2016665 (mouse) and PN 2016758 (keyboard) 2016962 Drive, diskette, 3.5 in., 1.44 MB, black 2016959 Drive, CD-ROM, 8X minimum, internal IDE 2016938...
Page 460 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-8 MCL Option Assembly (See Table 8.2-8) Figure Reference A Vertical plate components, Figure 8.2-9 Behind MCL Vertical Plate 7029163F 8.2-16 PN 4237029F...
Page 461 PARTS LISTS ILLUSTRATED PARTS Table 8.2-8 MCL Option Assembly (See Figure 8.2-8) Item Part Number Description 7000433 FRU, MCL option assembly 2016561 Card, Bar-Code Decoder 7000042 FRU, visible laser diode scanner (bar-code reader) Note: Attach the scanner head to the MCL vertical plate using four machine screws (#6-32 x 0.25 in.
Page 462 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-9 MCL Vertical Plate Components (See Table 8.2-9) 7029164F 8.2-18 PN 4237029F...
Page 463 PARTS LISTS ILLUSTRATED PARTS Table 8.2-9 MCL Vertical Plate Components (See Figure 8.2-9) Item Part Number Description 2515109 Spring, extension, 0.250 o.d. x 1.38 in. coiled length, 0.026 in. wire diameter, rated at 1.42 lbs/in. 7000439 FRU, probe sensor cable assembly 1021708 Sensor flag, MCL probe 1021652...
Page 464 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-10 Lifter Air Cylinder and Attachments (See Table 8.2-10) 7318083A Table 8.2-10 Lifter Air Cylinder and Attachments (See Figure 8.2-10) Item Part Number Description 6232591 Cylinder, lifter air, double acting, 250 psi, 0.56 bore, stud mount each end Note: If present, remove and discard the large nut on each end before installing.
Page 465 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-11 Probe Up/Down Air Cylinder and Attachments (See Table 8.2-11) 7318084A Table 8.2-11 Probe Up/Down Air Cylinder and Attachments (See Figure 8.2-11) Item Part Number Description 6232637 Choke, pneumatic, 0.0102 diameter, 0.062 barb and 10-32 threaded connections, brass Note: Choke must be oriented as shown when installation is complete.
Page 466 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-12 Front Display Panel (See Table 8.2-12) 5 (Rear view) 5 (Front view) 7029119F 8.2-22 PN 4237029F...
Page 467 PARTS LISTS ILLUSTRATED PARTS Table 8.2-12 Front Display Panel (See Figure 8.2-12) Item Part Number Description 1021769 Bracket, front panel 2839039 Screw, self-lock (#6-32 x 0.37 in. length, pan-head) 2827147 Washer, flat, #6 (0.156 i.d. x 0.312 o.d. x 0.036 in. thickness) 2827081 Washer, flat, #8 (0.172 i.d.
Page 468 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-13 Data Acquisition Card Cage (See Table 8.2-13) 7029005F 8.2-24 PN 4237029F...
Page 469 PARTS LISTS ILLUSTRATED PARTS Table 8.2-13 Data Acquisition Card Cage (See Figure 8.2-13) Item Part Number Description 6705318 Card, Cyto Transputer (also referred to as Cytometer Transputer card) 6705700 Card, MCL Interface 6705340 Card, System Interface 6705314 Card, Trans Data Acquisition 6705321 Card, Amp/Signal Conditioner Spare slot (used as a test slot in manufacturing)
Page 470 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-14 Upper Pneumatics Drawer (See Table 8.2-14) Figure Reference A VL22 (upper valve), Figure 8.2-15 B Bracket and fittings, Figure 8.2-16 C VL21 (lower valve), Figure 8.2-17 D Quick Disconnects (QD13 and QD14), Figure 8.2-18 ZERO RANGE 7029090F...
Page 471 PARTS LISTS ILLUSTRATED PARTS Table 8.2-14 Upper Pneumatics Drawer (See Figure 8.2-14) Item Part Number Description 7000375 FRU, upper pneumatics drawer assembly 7000192 FRU, regulator, electronic pressure (electronic transducer), for regulating sample pressure Note: Attach to posts using one #25 flat washer (0.265 i.d. x 0.484 o.d. x 0.027 in.
Page 472 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-15 VL22 - Exploded View of Upper Valve (See Table 8.2-15) 7029121F Table 8.2-15 VL22 - Exploded View of Upper Valve (See Figure 8.2-15) Item Part Number Description 6232086 Fitting, hose barb union, 0.062 i.d. to 10-32 threaded 2523062 O-ring, ethylene propylene seal, 0.187 i.d.
Page 473 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-16 Bracket and Fittings - Upper Pneumatics Drawer (See Table 8.2-16) 3 (4x) 2 (4x) 7029153F Table 8.2-16 Bracket and Fittings - Upper Pneumatics Drawer (See Figure 8.2-16) Item Part Number Description 6855212 Bracket, two pinch valve 1005697 Fitting, hose-barb union, 0.062 i.d.
Page 474 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-17 VL21 - Exploded View of Lower Valve (See Table 8.2-17) 7029120F Table 8.2-17 VL21 - Exploded View of Lower Valve (See Figure 8.2-17) Item Part Number Description 6232085 Fitting, hose barb union, 0125 i.d. to 10-32 threaded 2523062 O-ring, ethylene propylene seal, 0.187 i.d.
Page 475 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-18 Quick Disconnects Below Upper Pneumatics Drawer (See Table 8.2-18) QD14 QD13 empty empty 7 (Black, 6x) 6 (White, 1x) 4 (White, 1x) 5 (Black, 6x) 7029161F Table 8.2-18 Quick Disconnects Below Upper Pneumatics Drawer (See Figure 8.2-18) Item...
Page 476 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-19 Optical Collection Area, Rear View (See Table 8.2-19) Figure Reference A Front view (includes filter shield and filters), Figure 8.2-20 Front view with filter shield removed (includes flow cell and FALS detector), Figure 8.2-21 B Argon Laser (includes Power Supply and mounting hardware),...
Page 477 PARTS LISTS ILLUSTRATED PARTS Table 8.2-19 Optical Collection Area, Rear View (See Figure 8.2-19) Item Part Number Description 2815003 Screw, thumb, knurled nickel-plated brass (6-32 x 0.375 in. length) 7000352 FRU, side scatter diode 2839039 Screw, self-lock (#6-32 x 0.37 in. length, pan-head) 2827134 Washer, flat, #6 (0.156 i.d.
Page 478 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-20 Optical Collection Area, Front View (See Table 8.2-20) LASER RADIATION VISIBLE AND/OR INVISIBLE AVOID EXPOSURE TO BEAM LASER RADIATION WHEN OPEN CLASS 3B LASER PRODUCT AVOID EYE OR SKIN EXPOSURE TO DIRECT OR SCATTERED RADIATION Front view 7029127F 8.2-34...
Page 479 PARTS LISTS ILLUSTRATED PARTS Table 8.2-20 Optical Collection Area, Front View (See Figure 8.2-20) Item Part Number Description 2430348 Label, Class 3B Laser Product 2427785 Label, laser danger 2827147 Washer, flat, #6 (0.156 i.d. x 0.312 o.d. x 0.036 in. thickness) 2839039 Screw, self-lock (#6-32 x 0.37 in.
Page 480 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-21 Optical Collection Area with Filter Shield Removed (See Table 8.2-21) Figure Reference A Beamshaper assembly, Figure 8.2-22 B Fluorescence pickup lens and adjustment hardware, Figure 8.2-23 C Flow cell and associated hardware, Figure 8.2-23 Front view with filter shield removed 7029007F...
Page 481 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-22 Beam-Shaping Stage Components (See Table 8.2-22) Table 8.2-22 Beam-Shaping Stage Components (See Figure 8.2-22) Item Part Number Description 7000450 FRU, beamshaper 2 6859219 Lens holder, 10 mm 6859220 Lens holder, 80 mm 7000451 FRU, focus knob III 7000449 FRU, focus knob2 8.2-37...
Page 482 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-23 Flow Cell and Fluorescence Pickup Lens with Associated Hardware (See Table 8.2-23) 7029126F 8.2-38 PN 4237029F...
Page 483 PARTS LISTS ILLUSTRATED PARTS Table 8.2-23 Flow Cell and Fluorescence Pickup Lens with Associated Hardware (See Figure 8.2-23) Item Part Number Description 2851975 Screw, machine, knurled (#4-40 x 0.75 in. length, hex socket head) 1021703 Stage, snout support 1021682 Knob, snout 2807026 Screw, setscrew (#4-40 x 0.125 in length, hex-head) 2851982...
Page 484 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-24 Argon Laser, Power Supply, and Mounting Hardware (See Table 8.2-24) Rear view 7318047A 8.2-40 PN 4237029F...
Page 485 PARTS LISTS ILLUSTRATED PARTS Table 8.2-24 Argon Laser, Power Supply, and Mounting Hardware (See Figure 8.2-24) Item Part Number Description 6856941 Plenum, laser duct out 2840037 Fastener, ball stud 1022396 Screw, special laser mounting 2821010 Nut, self-lock (#6-32 x 0.250 AF x 0.109 in. thickness) 2827147 Washer, flat, #6 (0.156 i.d.
Page 486 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-25 Reagent Drawer (See Table 8.2-25) Figure Reference A Sheath liquid filter, Figure 8.2-26 B Sheath liquid filter purge (vent) connections, Figure 8.2-27 C Sheath container, Figure 8.2-28 D Cleaning agent container, Figure 8.2-29 Epics XL Epics XL MCL Blank 7029222F...
Page 487 PARTS LISTS ILLUSTRATED PARTS Table 8.2-25 Reagent Drawer (See Figure 8.2-25) Item Part Number Description 2523451 O-ring, silicone, used as rubber band, 2.300 i.d. x 0.103 width 7000677 FRU, reagent drawer with slides assembly 6807089 Panel, reagent drawer front, for XL flow cytometer, grey 6807088 Panel, reagent drawer front, for XL-MCL flow cytometer, grey 6855934...
Page 488 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-26 Sheath Liquid Filter (See Table 8.2-26) Top view Bottom view 7029109F 8.2-44 PN 4237029F...
Page 489 PARTS LISTS ILLUSTRATED PARTS Table 8.2-26 Sheath Liquid Filter (See Figure 8.2-26) Item Part Number Description 6912942 Assembly, sheath filter 6232472 Quick-connect, male, external elbow, 0.250 o.d., white acetal delrin 6232522 Quick-connect, male, external elbow, 0.375 o.d., white acetal delrin 8.2-45 PN 4237029F...
Page 490 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-27 Sheath Liquid Filter Purge (Vent) Connections (See Table 8.2-27) (Comes with item 7) 7029142F 8.2-46 PN 4237029F...
Page 491 PARTS LISTS ILLUSTRATED PARTS Table 8.2-27 Sheath Liquid Filter Purge (Vent) Connections (See Figure 8.2-27) Item Part Number Description 2807026 Screw, setscrew (#4-40 x 0.125 in length, hex-head) 1018728 Knob, pinch valve 2822033 Nut, hex (47-32 UNS x 0.562 AF x 0.078 in. thickness) Washer, I -tooth, #47, 0.47 i.d.
Page 492 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-28 Sheath Container (See Table 8.2-28) 7318036A Table 8.2-28 Sheath Container (See Figure 8.2-28) Item Part Number Description 7000378 FRU, sheath tank with sensor assembly 6232472 Quick-connect, male, external elbow, 0.250 o.d., white acetal delrin 6232470 Quick-connect, female, internal panel mount, 0.125 i.d., coupling pair latch, barb, white acetal delrin...
Page 493 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-29 Cleaning Agent Container (See Table 8.2-29) 7318035A Table 8.2-29 Cleaning Agent Container (See Figure 8.2-29) Item Part Number Description 7000379 FRU, cleanse tank with sensor assembly 6232472 Quick-connect, male, external elbow, 0.250 o.d., white acetal delrin 6232470 Quick-connect, female, internal panel mount, 0.125 i.d., coupling pair latch, barb, white acetal delrin...
Page 494 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-30 Sample Station (Manual) for XL-MCL Cytometer, Front View (See Table 8.2-30) 7029170F Note: See Figure 8.2-31 for the rear view of the manual sample station on an XL-MCL flow cytometer. 8.2-50 PN 4237029F...
Page 495 PARTS LISTS ILLUSTRATED PARTS Table 8.2-30 Sample Station (Manual) for XL-MCL Cytometer, Front View (See Figure 8.2-30) Item Part Number Description 7000678 FRU, manual sample station, for XL-MCL Cytometer with grey covers 7000354 FRU, manual sample station, for XL-MCL Cytometer with black covers 6807081 Sample station, for an XL-MCL with grey covers 6858518...
Page 496 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-31 Sample Station (Manual) for XL-MCL Cytometer, Rear View (See Table 8.2-31) 7029168F Note: See Figure 8.2-30 for the front view of the manual sample station on an XL-MCL flow cytometer. 8.2-52 PN 4237029F...
Page 497 PARTS LISTS ILLUSTRATED PARTS Table 8.2-31 Sample Station (Manual) for XL-MCL Cytometer, Rear View (See Figure 8.2-31) Item Part Number Description 7000678 FRU, manual sample station, for XL-MCL Cytometer with grey covers 7000354 FRU, manual sample station, for XL-MCL Cytometer with black covers 2851859 Latch, concealed pull-up catch and keeper, 1.9 x 1.1 x 0.3 in., black nylon Note: Only the latch catch is shown in...
Page 498 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-32 Sample Station for XL Cytometer (See Table 8.2-32) Rear view 7029165F 8.2-54 PN 4237029F...
Page 499 PARTS LISTS ILLUSTRATED PARTS Table 8.2-32 Sample Station for XL Cytometer (See Figure 8.2-32) Item Part Number Description 7000679 FRU, sample station assembly, for XL Cytometer with grey covers FRU, sample station assembly, for XL Cytometer with black covers 7000360 Note: Before attaching sample station to the Cytometer, make sure the flow cell cover and filter shield are attached to the Cytometer.
Page 500 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-33 Sample Station for XL-MCL and XL Cytometers - Mechanical Assembly (See Table 8.2-33) J115 7029162F 8.2-56 PN 4237029F...
Page 501 PARTS LISTS ILLUSTRATED PARTS Table 8.2-33 Sample Station for XL-MCL and XL Cytometers - Mechanical Assembly (See Figure 8.2-33) Item Part Number Description 1020914 Shaft, 0.375 square 1020873 Arm. probe 2827147 Washer, flat, #6 (0.156 i.d. x 0.312 o.d. x 0.036 in. thickness) 2826035 Washer, split-lock, #6 (0.14 i.d.
Page 502 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-34 Segmenting Valve Assembly (See Table 8.2-34) Figure Reference A Air cylinder and related hardware for segmenting valve middle pad, Figure 8.2-26 B Air cylinder and related To manual hardware for segmenting sample probe valve front and rear pads, Figure 8.2-27 To MCL sample probe...
Page 503 PARTS LISTS ILLUSTRATED PARTS Table 8.2-34 Segmenting Valve Assembly (See Figure 8.2-34) Item Part Number Description 7000370 FRU, segmenting valve assembly 7000195 FRU, segmenting valve rear pad 7000191 FRU, segmenting valve middle pad 7000196 FRU, segmenting valve front pad 6912941 Kit, Sample/MCL Intro Line 1021636 Tubing, PEEK, manual sample intro line, 14 in.
Page 504 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-35 Air Cylinder and Related Hardware for Segmenting Valve Middle Pad (See Table 8.2-35) 7029158F Table 8.2-35 Air Cylinder and Related Hardware for Segmenting Valve Middle Pad (See Figure 8.2-35) Item Part Number Description 6216002 Fitting, miniature, 10-32 threaded, 10-32 tap, adjustable, brass 2523062 O-ring, ethylene propylene seal, 0.187 i.d.
Page 505 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-36 Air Cylinder and Related Hardware for Front and Rear Segmenting Valve Pads (See Table 8.2-36) 7029159F 7029159F Table 8.2-36 Air Cylinder and Related Hardware for Front and Rear Segmenting Valve Pads (See Figure 8.2-36) Item Part Number Description...
Page 506 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-37 Lower Pneumatics Drawer (See Table 8.2-37) Figure Reference A Pinch valve and associated components, Figure 8.2-38 B Solenoid manifold, Figure 8.2-39 C QD10 and QD11 (coupling and fittings), Figure 8.2-40 D Quick-disconnect fittings and brackets, Figure 8.2-41 7029102F 8.2-62...
Page 507 PARTS LISTS ILLUSTRATED PARTS Table 8.2-37 Lower Pneumatics Drawer (See Figure 8.2-37) Item Part Number Description 7000374 FRU, lower pneumatics drawer assembly 6232492 Valve, electro-pneumatic, solenoid and pilot actuator combination valve, 24 Vdc / 30 psi, 4.5 lb pinch force, see Figure 8.2-38 for an exploded view that includes related components...
Page 508 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-38 Pinch Valve and Associated Components, Lower Pneumatics Drawer (See Table 8.2-38) VL0 and VL7 VL13 7029201F 8.2-64 PN 4237029F...
Page 509 PARTS LISTS ILLUSTRATED PARTS Table 8.2-38 Pinch Valve and Associated Components, Lower Pneumatics Drawer (See Figure 8.2-38) Item Part Number Description 6232814 Fitting, T-connector, adjustable, 3/32 i.d. hose barb to 3/32 i.d. hose barb to 10-32 threaded, brass miniature 6232813 Fitting, elbow, adjustable, 3/32 i.d.
Page 510 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-39 Solenoid Manifold, Lower Pneumatics Drawer (See Table 8.2-39) 7029203F Table 8.2-39 Solenoid Manifold, Lower Pneumatics Drawer (See Figure 8.2-39) Item Part Number Description 6232587 Assembly, solenoid valve, pressure relief valves and manifold 6216129 Fitting, T-connector, hose barb, 0.187 i.d. to 0.187 i.d. to threaded, brass Note: Apply a thin line of pipe sealant, PN 1601056, across threads prior to insertion.
Page 511 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-40 QD10 and QD11 (Coupling and Fittings), Lower Pneumatics Drawer (See Table 8.2-40) QD10 QD11 empty empty 6 (White, 6x) 5 (Black, 2x) 3 (White, 3x) 4 (Black, 5x) 7029202F Table 8.2-40 QD10 and QD11 (Coupling and Fittings), Lower Pneumatics Drawer (See Figure 8.2-40) Item...
Page 512 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-41 Quick-Disconnect Fittings and Brackets, Lower Pneumatics Drawer (See Table 8.2-41) 4 (16x) QD42 QD41 QD40 QD39 QD38 QD45 QD44 QD43 QD49 QD48 QD47 QD46 QD54 QD53 QD52 QD51 QD50 QD58 QD57 QD56 QD55 QD63 QD62 QD61 QD60 QD59...
Page 513 PARTS LISTS ILLUSTRATED PARTS Table 8.2-41 Quick-Disconnect Fittings and Brackets, Lower Pneumatics Drawer (See Figure 8.2-41) Item Part Number Description 2839039 Screw, self-lock (#6-32 x 0.37 in. length, pan-head) 6011019 Mount, wire tie, 0.51 in. length x 0.33 in. width, for use with #6 screw 6232799 Fitting, insert, white female quick-connect, internal connector to 0.094 i.d.
Page 514 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-42 Cytometer Rear Panel, Lower (See Table 8.2-42) 8.2-70 PN 4237029F...
Page 515 PARTS LISTS ILLUSTRATED PARTS Table 8.2-42 Cytometer Rear Panel, Lower (See Figure 8.2-42) Item Part Number Description 2603025 Fan, box, 106 CFM, 24 Vdc (4.68 square x 1.5 thickness) Note: To ensure proper cable length, verify fan orientation before attaching the fan to the Cytometer frame.
Page 516 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-43 Fan Assembly, Cytometer Rear Panel (See Table 8.2-43) Air flow into unit Fan to shock mount Shock mount to Cytometer frame 7318048A 8.2-72 PN 4237029F...
Page 517 PARTS LISTS ILLUSTRATED PARTS Table 8.2-43 Fan Assembly, Cytometer Rear Panel (See Figure 8.2-43) Item Part Number Description 2821010 Nut, self-lock (#6-32 x 0.250 AF x 0.109 in. thickness) 2827147 Washer, flat, #6 (0.156 i.d. x 0.312 o.d. x 0.036 in. thickness) 2603053 Fan, shock mount, for 4-inch fan, black 2603025...
Page 518 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-44 Cytometer Right Side Compartment (See Table 8.2-44) 7318051A 8.2-74 PN 4237029F...
Page 519 PARTS LISTS ILLUSTRATED PARTS Table 8.2-44 Cytometer Right Side Compartment (See Figure 8.2-44) Item Part Number Description 6232086 Fitting, hose barb union, 0.062 i.d. to 10-32 threaded 2523062 O-ring, ethylene propylene seal, 0.187 i.d. x 0.050 wall 6232085 Fitting, hose barb union, 0.125 i.d. to 10-32 threaded 1018616 Manifold rear panel 2104365...
Page 520 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-45 Power Supply Module - Left Side with Cover Removed (See Table 8.2-45) P-73 SN13 CB-1 7029155F 8.2-76 PN 4237029F...
Page 521 PARTS LISTS ILLUSTRATED PARTS Table 8.2-45 Power Supply Module - Left Side with Cover Removed (See Figure 8.2-45) Item Part Number Description 7000431 Power supply, Argon air-cooled laser, switching, for 100 Vac system 7000721 Power supply, Argon air-cooled laser, switching, for 115Vac system 7000432 Power supply, Argon air-cooled laser, switching, for 220 or 240 Vac system 7000371...
Page 522 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-46 VL32, VL30, and VL31 - Exploded View (See Table 8.2-46) 7318046A 8.2-78 PN 4237029F...
Page 523 PARTS LISTS ILLUSTRATED PARTS Table 8.2-46 VL32, VL30, and VL31 - Exploded View (See Figure 8.2-46) Item Part Number Description 6216128 Fitting, elbow, hose barb, 0.187 i.d. to 1/8 MPT, brass Note: Apply a thin line of pipe sealant, PN 1601056, across threads prior to insertion.
Page 524 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-47 Power Supply Module - Front Panel Components (See Table 8.2-47) FILTER WATER TRAP TRAP SYSTEM POWER FILTER MCL POWER PRESS PRESS 7029134F 8.2-80 PN 4237029F...
Page 525 PARTS LISTS ILLUSTRATED PARTS Table 8.2-47 Power Supply Module - Front Panel Components (See Figure 8.2-47) Item Part Number Description 2603058 Fan, box, 90 CFM, 24 Vdc, 4.69 square x 1.0 in. thickness 6232724 Trap, vacuum, with miniature polycarbonate bowl, see Figure 8.2-48 for an exploded view that includes attachments...
Page 526 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-48 Vacuum Trap and Related Components (See Table 8.2-48) 7029135F Table 8.2-48 Vacuum Trap and Related Components (See Figure 8.2-48) Item Part Number Description 6216128 Fitting, hose barb, elbow, 0.187 i.d. to 1/8 MNPT, brass Note: Apply a thin line of pipe sealant, PN 1601056, across threads prior to insertion.
Page 527 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-49 Water Trap (Air/Water Filter Separator) and Related Components (See Table 8.2-49) 7029136F Table 8.2-49 Water Trap (Air/Water Filter Separator) and Related Components (See Figure 8.2-49) Item Part Number Description 6216128 Fitting, hose barb, elbow, 0.187 i.d. to 1/8 MNPT, brass Note: Apply a thin line of pipe sealant, PN 1601056, across threads prior to insertion.
Page 528 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-50 Front Panel including Voltage Supply Monitor Card and Hardware (See Table 8.2-50) 7029137F Table 8.2-50 Front Panel including Voltage Supply Monitor Card and Hardware (See Figure 8.2-50) Item Part Number Description 2821010 Nut, self-lock (#6-32 x 0.250 AF x 0.109 in. thickness) 2827147 Washer, flat, #6 (0.156 i.d.
Page 529 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-51 System Pressure Gauge and Related Components (See Table 8.2-51) 7029138F Table 8.2-51 System Pressure Gauge and Related Components (See Figure 8.2-51) Item Part Number Description 6232214 Fitting, hose barb, elbow, 0.187 i.d. to 1/8 FPT, nickel-plated brass Note: Fitting must be oriented as shown after tightening.
Page 530 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-52 System Pressure Adjustment Knob and Related Components (See Table 8.2-52) 7029139F Table 8.2-52 System Pressure Adjustment Knob and Related Components (See Figure 8.2-52) Item Part Number Description 6232501 Muffler, noise reducing pneumatic, 0.125 MNPT, 0.812 diameter, 2.125 length 6216127 Fitting, T-connector, hose barb, 0.187 i.d.
Page 531 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-53 Vacuum and Air Filters with Related Components (See Table 8.2-53) 7029140F Table 8.2-53 Vacuum and Air Filters with Related Components (See Figure 8.2-53) Item Part Number Description 6232702 Quick-connect, male, elbow, 0.250 o.d., white acetal delrin 6232703 Quick-connect, female, 0.125 flow, 0.375 MNPT, white acetal delrin, single connect...
Page 532 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-54 System Vacuum Gauge and Related Components (See Table 8.2-54) 7029141F Table 8.2-54 System Vacuum Gauge and Related Components (See Figure 8.2-54) Item Part Number Description 6232214 Fitting, hose barb, elbow, 0.187 i.d. to 1/8 FPT, nickel-plated brass Note: Fitting must be oriented as shown after tightening.
Page 533 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-55 Power Supply Module - Right Side with Cover Removed (See Table 8.2-55) 7029167F Table 8.2-55 Power Supply Module - Right Side with Cover Removed (See Figure 8.2-55) Item Part Number Description 5110031 Interlock, high voltage (HV), snap action cheat switch 7000357 FRU, Power Supply, +24 Vdc 6856536...
Page 534 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-56 Waste Container (See Table 8.2-56) 7318034A 8.2-90 PN 4237029F...
Page 535 PARTS LISTS ILLUSTRATED PARTS Table 8.2-56 Waste Container (See Figure 8.2-56) Item Part Number Description 2523697 Tank, waste, I-gallon rectangular (also referred to as bottle) 6858159 Sensor assembly, waste level 2428039 Label, waste container 8.2-91 PN 4237029F...
Page 536 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-57 Power Supply Module - Rear Panel Components (See Table 8.2-57) 8.2-92 PN 4237029F...
Page 537 PARTS LISTS ILLUSTRATED PARTS Table 8.2-57 Power Supply Module - Rear Panel Components (See Figure 8.2-57) Item Part Number Description 6706391 Card, CYT12 Receiver, EMC Note: Attach using hex nut, PN 2851995 (0.25-36 UNS x 0.375 AF x 0.094 in. thickness). 6028693 Cable, harness that includes Logic, MCL, and Analog power supply dc internal cables...
Page 538 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-58 Box Fan with Related Hardware (See Table 8.2-58) 6 (4x) 2 (8x) 5 (8x) 7029156F Table 8.2-58 Box Fan with Related Hardware (See Figure 8.2-58) Item Part Number Description 2603010 Filter, air, 4-inch pad, 45 PPI 2806073 Screw, machine (#6-32 x 0.62 in.
Page 539 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-59 Check Valves and Chokes with Related Components (See Table 8.2-59) 7029218F Table 8.2-59 Check Valves and Chokes with Related Components (See Figure 8.2-59) Item Part Number Description 6214106 Valve, check, 0.156 i.d. to 0.156 i.d. tubing, designated as CV 1, 3, 6, 9, and 10 on page 1 of the XL System Pneumatic / Hydraulic Layout, PN 6320886 6232605 Valve, check, 0.125 i.d.
Page 540 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-60 Fittings (See Table 8.2-60) 5, 6 7029219F Table 8.2-60 Fittings (See Figure 8.2-60) Item Part Number Description 1005699 Fitting, feed-thru, hose-barb union, metal, 0.093 i.d. to 0.093 i.d. with 10-32 threads 1016486 Fitting, feed-thru isolator, white, with 10-32 threads 6216353 Fitting, feed-thru, hose-barb union, 0.062 i.d.
Page 541 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-61 Tie Wraps and Mounting Hardware (See Table 8.2-61) 7029169F Note: For laser mounting hardware, go to the illustration for the laser. Table 8.2-61 Tie Wraps and Mounting Hardware (See Figure 8.2-61) Item Part Number Description 6027284 Mount, wire tie, 0.75 x 0.75, with #6 hole, adhesive back...
Page 542 PARTS LISTS ILLUSTRATED PARTS Figure 8.2-62 Flexible Duct Components and Assembly Instructions (See Table 8.2-62) Step 1 Step 2 Remove outer covering Roll insulation tightly and trim the width of around flex duct. the insulation 8" Step 3 Step 4 Place first Outer covering...
Page 543 CONTENTS QUICK REFERENCE INFORMATION, A.1-1 TOLERANCES AND LIMITS, A.1-1 MCL CPU Card, A.1-1 CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS, A.2-1 Amp / Signal Conditioner Card, A.2-2 Circuit Card Locations in the Cytometer, A.2-2 Component Locations, A.2-2 Jumpers, A.2-2 Switches, A.2-2 Analyzer Backplane - Front View, A.2-3 Backplane Location in the...
Page 544 CONTENTS MCL CPU Card, A.2-11 Circuit Card Location in the Cytometer, A.2-11 Component Locations, A.2-11 Jumpers, A.2-11 Switches, A.2-11 MCL Interface Card, A.2-12 Circuit Card Location in the Cytometer, A.2-12 Component Locations, A.2-12 Jumpers, A.2-12 Switches, A.2-12 Opto Transprocessor EXMEM Card (Non-EMC Version), A.2-13 Circuit Card Location in the Workstation, A.2-13...
Page 545 CONTENTS Switches, A.2-20 Top Panel Display 2 Card, A.2-21 Circuit Card Location in the Cytometer, A.2-21 Component Locations, A.2-21 Jumpers, A.2-21 Switches, A.2-21 Trans Data Acquisition Card, A.2-22 Circuit Card Location in the Cytometer, A.2-22 Component Locations, A.2-22 Jumpers, A.2-22 Switches, A.2-22 Voltage Selector Card, A.2-23...
Page 546 CONTENTS Carousel Components, A.5-42 MCL Components Accessible with Covers Removed, A.5-44 Components in the Left Side of the Cytometer, A.5-47 Front Panel Display Components, A.5-55 POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS, A.6-1 Overview, A.6-1 Main Power Supply Module Components, A.6-1 Locating a Component, A.6-1 Power Supply Module Anchor...
Page 547 CONTENTS Power Mgmt (Power Management) Setup, B.2-4 PCI / PnP Setup, B.2-5 Peripheral Setup, B.2-6 Supervisor, B.2-6 WINDOWS 95 Configuration from Device Manager, B.2-7 Control Panel Setup, Device Manager, Computer, B.2-7 CONFIG.SYS Configuration, B.2-8 AUTOEXEC.BAT Configuration, B.2-9 ® ® INTEL PENTIUM 166 PROCESSOR CONFIGURATION, B.3-1...
Page 548 CONTENTS NETWORK PROTOCOL SPECIFICATIONS, E.1-1 802.3, E.1-1 BAR-CODE SPECIFICATIONS, F .1-1 F .1 BAR-CODE LABELS, F .1-1 Acceptable Bar Codes, F .1-1 Optical Characteristics of Bar-Code Labels, F .1-1 NE (Narrow Elements) Width, F .1-2 WE/NE (Wide Elements/Narrow Elements) Ratio, F .1-2 Printing Methods, F .1-2 Check Sum...
Page 549 CONTENTS A.2-12 Opto Transprocessor EXMEM Card (Non-EMC Version) - Component Locations, A.2-13 A.2-13 Opto Transprocessor EXMEM II Card (EMC Version) - Component Locations, A.2-14 A.2-14 PMT Distribution and Laser Fan Control Card - Component Locations, A.2-15 A.2-15 Power Module Control Card (Non-EMC Version) - Component Locations, A.2-16 A.2-16 Power Module Control II Card (EMC Version) - Component Locations, A.2-17...
Page 550 CONTENTS A.3-3 Protocol to Acquire Fluorospheres, A.3-2 A.4-1 Tests on Prefinal Software Diskette, A.4-1 A.4-2 MCL Option Commands, A.4-4 A.5-1 XL Cytometer with MCL Option, Component and Assembly Accessibility, A.5-3 A.5-2 Components Accessed with the Cytometer Top Cover Removed and Their Functions, A.5-6 A.5-3 Data Acquisition Card Cage, Components and Functions, A.5-8...
Page 551 QUICK REFERENCE INFORMATION TOLERANCES AND LIMITS MCL CPU Card The MCL voltages are measured at the MCL CPU card, but adjustments are made ATTENTION: on the corresponding MCL power supply in the right-side compartment of the Power Supply module. For the MCL power supply locations, refer to Figure A.6-4.
Page 552 QUICK REFERENCE INFORMATION TOLERANCES AND LIMITS A.1-2 PN 4237029F...
Page 553 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS This appendix contains key component and test point locations as well as applicable jumper and switch settings for the following circuit cards: Card Name Figure Reference Amp / Signal Conditioner Card Figure A.2-1 Analyzer Backplane - Front View...
Page 554 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Amp / Signal Conditioner Card Circuit Card Locations in the Cytometer On a four-color system, Amp / Signal Conditioner cards occupy seven slots in the Data Acquisition card cage. Only six slots are used if it is a three-color system. This circuit card is used in the slots labeled (left to right) PMT4 (missing in a three-color system),...
Page 555 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Analyzer Backplane - Front View Backplane Location in the Cytometer The Analyzer backplane is attached to the rear of the Data Acquisition card cage. Most of this backplane is behind the Data Acquisition card cage so that only those components on the lower portion of this backplane are easily accessible.
Page 556 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Analyzer Backplane - Rear View Backplane Location in the Cytometer The Analyzer backplane is attached to the rear of the Data Acquisition card cage. To access the rear of the Analyzer backplane, remove the Cytometer front door and lock the Data Acquisition card cage in its upright position outside the Cytometer.
Page 557 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Bar-Code Decoder Card ATTENTION: This card is present only when the MCL option is installed. Circuit Card Location in the Cytometer The Bar-Code Decoder card is the smaller of the two circuit cards attached to the MCL main frame on the left-side of the Cytometer.
Page 558 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS CYT12 Receiver EMC Card ATTENTION: This circuit card is only used in XL and XL-MCL instruments with the serial number Z09063 or higher. XL and XL-MCL instruments with the serial number Z09062 or lower do not use this circuit card.
Page 559 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Cyto Transputer Card Circuit Card Location in the Cytometer The Cyto (Cytometer) Transputer card occupies the slot labeled in the Data CYTO TRANS PROC Acquisition card cage. To locate the Cyto Transputer card, see Figure A.5-3.
Page 560 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Fiber Optic Interface Card Circuit Card Location in the Cytometer The Fiber Optic Interface card is attached to the lower rear panel inside the Cytometer. To locate the Fiber Optic Interface card, see Figure A.5-9.
Page 561 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Front Panel LED and Switch Input Card ATTENTION: This card is present only when the MCL option is not installed. Circuit Card Location in the Cytometer The Front Panel LED and Switch Input card accepts data entry information when the overlay membrane at the manual sample station on an XL flow cytometer is pressed.
Page 562 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Front Panel LED and Switch Input 2 Card ATTENTION: This card is present only when the MCL option is installed. Circuit Card Location in the Cytometer The Front Panel LED and Switch Input 2 card accepts data entry information when the overlay membrane at the manual sample station on an XL-MCL flow cytometer is pressed.
Page 563 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS MCL CPU Card ATTENTION: This card is present only when the MCL option is installed. Circuit Card Location in the Cytometer The MCL CPU card is the larger of the two circuit cards attached to the MCL main frame on the left-side of the Cytometer.
Page 564 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS MCL Interface Card ATTENTION: This card is present only when the MCL option is installed. Circuit Card Location in the Cytometer The MCL Interface card occupies the slot labeled MCL INFC in the Data Acquisition card cage.
Page 565 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Opto Transprocessor EXMEM Card (Non-EMC Version) Note: Opto Transprocessor EXMEM is short for Optical Transprocessor Extended Memory card. This circuit card is used in all XL and XL-MCL instruments serial number Z09062 and below.
Page 566 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Opto Transprocessor EXMEM II Card (EMC Version) Note: Opto Transprocessor EXMEM II is short for Optical Transprocessor Extended Memory II card. This circuit card is used in all XL and XL-MCL instruments serial number Z09063 and above.
Page 567 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS PMT Distribution and Laser Fan Control Card Circuit Card Location in the Cytometer The PMT Distribution and Laser Fan Control card is attached to the optical bench inside the Cytometer. To locate the PMT Distribution and Laser Fan Control card, see Figure A.5-4.
Page 568 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Power Module Control Card (Non-EMC Version) Note: The Power Module Control card is used in XL and XL-MCL instruments with a serial number Z09062 or lower. Circuit Card Location in the Power Supply Module The Power Module Control card is inside the Power Supply module.
Page 569 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Power Module Control II Card (EMC Version) Note: The Power Module Control II card is used in XL and XL-MCL instruments with a serial number Z09063 or higher. Circuit Card Location in the Power Supply Module The Power Module Control II card is inside the Power Supply module.
Page 570 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Sensor Card Circuit Card Location in the Cytometer The Sensor card is located inside the upper pneumatics drawer. To locate the Sensor card, see Figure A.5-5. Component Locations Figure A.2-17 Sensor Card - Component Locations Sample Pressure sensor PWB 1712902E...
Page 571 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Solenoid Power Distribution Card Circuit Card Location in the Cytometer The Solenoid Power Distribution card is located inside the lower pneumatics drawer. To locate the Solenoid Power Distribution card, see Figure A.5-15.
Page 572 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS System Interface Card Circuit Card Location in the Cytometer The System Interface card occupies the slot labeled in the Data Acquisition card SYS INFC cage. To locate the System Interface card, see Figure A.5-3.
Page 573 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Top Panel Display 2 Card Circuit Card Location in the Cytometer The Top Panel Display 2 card is attached to the back of the front display panel. To access this circuit card the front display panel must be lifted. To locate the Top Panel Display 2 card, see Figure A.5-16.
Page 574 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Trans Data Acquisition Card Circuit Card Location in the Cytometer The Trans Data Acquisition card occupies the slot labeled in the Data Acquisition DATA AQC card cage. To locate the Trans Data Acquisition card, see Figure A.5-3.
Page 575 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Voltage Selector Card Circuit Card Location in the Power Supply Module The Voltage Selector card is inside the Power Supply module. When the three-sided cover is removed from the Power Supply module, the Voltage Selector card can be accessed in the upper compartment.
Page 576 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Component Locations Figure A.2-22 Voltage Selector Card for 120 Vac System - Component Locations PWB 1712909C 7029188F Jumpers Switches A.2-24 PN 4237029F...
Page 577 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS Voltage Supply Monitor Card Circuit Card Location in the Power Supply Module The Voltage Supply Monitor card is inside the Power Supply module. When the three-sided cover is removed from the Power Supply module, the Voltage Supply Monitor card can be accessed from the right side.
Page 578 QUICK REFERENCE INFORMATION CIRCUIT CARD LAYOUTS WITH KEY COMPONENTS A.2-26 PN 4237029F...
Page 579 QUICK REFERENCE INFORMATION PROTOCOL PARAMETERS PROTOCOL PARAMETERS CHANNEL 500 Protocol Table A.3-1 CHANNEL 500 Protocol Histogram Parameter Signals Gating Analysis Region Dual FS vs. SS RECT GATE Single On region A Single On region A Single On region A Single On region A Single On region A...
Page 580 QUICK REFERENCE INFORMATION PROTOCOL PARAMETERS Acquiring Fluorospheres Table A.3-3 Protocol to Acquire Fluorospheres Histogram Parameter Signals Gating Analysis Region Dual FS vs. SS RECT GATE Single On region A Single On region A Single On region A Single On region A Single On region A Single...
Page 581 QUICK REFERENCE INFORMATION PREFINAL SERVICE SOFTWARE PREFINAL SERVICE SOFTWARE Table A.4-1 provides a quick reference of available tests that may be accessed when the Prefinal Service software is installed and operated using the guidelines provided under Heading 4.2, PREFINAL SERVICE SOFTWARE INSTALLATION AND OPERATION.
Page 582 QUICK REFERENCE INFORMATION PREFINAL SERVICE SOFTWARE Table A.4-1 Tests on Prefinal Software Diskette (Continued) Test Function MCL Carousel Status Checks quickly for carousel alignment. MCL Door Switch Test Checks for proper operation of MCL option door switch. MCL Finger Test Allows proper adjustment of finger rotator.
Page 583 QUICK REFERENCE INFORMATION PREFINAL SERVICE SOFTWARE Table A.4-1 Tests on Prefinal Software Diskette (Continued) Test Function T805 Test Tests to verify proper T805 chip installed on Opto Transprocessor EXMEM or Opto Transprocessor EXMEM II card. Note: Failure of this test may indicate the circuit card has an INS425/INS405 transputer (instead of the INS805 transputer).
Page 584 QUICK REFERENCE INFORMATION PREFINAL SERVICE SOFTWARE MCL Option Commands Table A.4-2 MCL Option Commands Name Keystrokes Action Èmr Master Reset Place carousel on turntable before executing this command. Always execute this command before any other command. Resets MCL option, brings carousel out, places it in home position. Èch Carousel Home Places carousel number label in front of bar-code scanner.
Page 585 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Overview Most Cytometer functions are accomplished by fluidic components that are interconnected by tubing and controlled by timed solenoid signals. This section briefly describes the functions of these fluidic components and shows their locations. Main Cytometer Components To expedite finding the name, location, or description of a component, the illustrations and tables in this section are organized in functional groups.
Page 586 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Cytometer Anchor Illustration Figure A.5-1 XL Cytometer with MCL Option, Anchor Illustration for Locating Figure Reference Components A Components accessed with the top cover removed, Figure A.5-2 r Interlock switch, Figure A.5-2 r Data Acquisition card cage, Figure A.5-3...
Page 587 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-1 XL Cytometer with MCL Option, Component and Assembly Accessibility Figure Reference Main Component or Assembly that is Accessible To Access Component or Assembly A.5-1, A Data Acquisition card cage Must remove the top cover. Optical collection area r Argon laser head, plenum, duct r Laser cooling fans...
Page 588 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-1 XL Cytometer with MCL Option, Component and Assembly Accessibility (Continued) Figure Reference Main Component or Assembly that is Accessible To Access Component or Assembly A.5-1, F Data entry membrane switches Exterior access.
Page 589 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Components Accessed with the Cytometer Top Cover Removed The Cytometer interlock switch and Data Acquisition Card Cage are accessible when the Cytometer top cover is removed. The rear section of the Optical Collection Area is accessible when the Cytometer top cover is removed and the Data Acquisition card cage is removed from the center cavity.
Page 590 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-2 Components Accessed with the Cytometer Top Cover Removed and Their Functions Figure Reference Reference Component Function Designator A.5-2, 1 Data Acquisition card cage Houses the electronic circuit cards inside the Cytometer (Figure A.5-3).
Page 591 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Data Acquisition Card Cage The Data Acquisition card cage is accessible when the Cytometer top cover is removed. However, most procedures require the card cage be lifted out of the Cytometer and locked in its vertical position.
Page 592 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-3 Data Acquisition Card Cage, Components and Functions Figure Reference Reference Component Function Designator A.5-3, 1 Cyto Transputer card Circuit card located in Slot 1 of the Data Acquisition card Refer to cage (left to right orientation).
Page 593 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-3 Data Acquisition Card Cage, Components and Functions (Continued) Figure Reference Reference Component Function Designator A.5-3, 6 FS Amp/Signal Conditioner Circuit card located in Slot 6 of the Data Acquisition card Refer to card cage (left to right orientation).
Page 594 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-3 Data Acquisition Card Cage, Components and Functions (Continued) Figure Reference Reference Component Function Designator A.5-3, 9 PMT4 Amp/Signal Conditioner Circuit card is located in Slot 9 of the Data Acquisition card Refer to card (optional) cage (left to right orientation) only when the four-color...
Page 595 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-3 Data Acquisition Card Cage, Components and Functions (Continued) Figure Reference Reference Component Function Designator A.5-3, 13 PMT4 HV power supply In the HV power supply section of the Data Acquisition (optional) card cage, the PMT4 HV power supply is located in the upper left quadrant when the four-color option is installed.
Page 596 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-3 Data Acquisition Card Cage, Components and Functions (Continued) Figure Reference Reference Component Function Designator A.5-3, 16 PMT2 HV power supply In the HV power supply section of the Data Acquisition card cage, the PMT2 HV power supply is located in the lower right quadrant.
Page 597 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Optical Collection Area The rear section of the optical collection area is accessible when the Cytometer top cover is removed and the Data Acquisition card cage is removed from the center cavity Optical Collection Area, Component Locations (Rear View) Figure A.5-4 Optical Collection Area, Rear View (See Table...
Page 598 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-4 Optical Collection Area, Components and Functions Figure Reference Reference Component Function Designator A.5-4, 1 Laser blower assembly Assembly contains two fans that force cooling air across the Argon laser head. A.5-4, 2 Laser duct out plenum Facilitates cooling of the laser head by directing the forced...
Page 599 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-4 Optical Collection Area, Components and Functions (Continued) Figure Reference Reference Component Function Designator A.5-4, 9 PMT1 Photomultiplier tube that detects the 525 nm wavelength emitted by the FITC dye (fluorescein isothiocyanate dye). Light entering PMT1 is converted to a Gaussian-shaped analog pulse that is amplified in response to the PMT1 HV gain setting defined by the operator.
Page 600 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Components Accessed with the Right Side Cover Removed Figure A.5-5 Right Side View of the Cytometer with the Cover Removed (See Table A.5-5) ZER O RANGE 1. Water trap filter 9. SN8 2.
Page 601 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-5 Components in the Right Side of the Cytometer and their Functions Figure Reference Reference Component Function Designator A.5-5, 1 Water trap filter Component may also be referred to as an air/water filter FL 5 inside separator.
Page 602 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-5 Components in the Right Side of the Cytometer and their Functions (Continued) Figure Reference Reference Component Function Designator A.5-5, 4 Cooling coil Copper coil that provides a passageway for the once COOLING cooled compressed air (pressure) from the Power Supply COIL inside...
Page 603 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-5 Components in the Right Side of the Cytometer and their Functions (Continued) Figure Reference Reference Component Function Designator A.5-5, 8 Normally-closed vacuum/pressure switch monitors the SN 9 inside system pressure available to the Cytometer. (This is the UPPER system pressure generated by the pressure portion of the PNEUMATICS...
Page 604 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-5 Components in the Right Side of the Cytometer and their Functions (Continued) Figure Reference Reference Component Function Designator A.5-5, 13 QD14 Quick-disconnect male coupling that provides one central QD 14 left of connector for routing pneumatics to components inside UPPER the upper pneumatics drawer and then routing regulated...
Page 605 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Components Accessed with the Center Front Cover Removed The center front cover may also be referred to as the filter cover or center front panel. Figure A.5-6 Inside View of the Cytometer with the Center Front Cover Removed (See Table A.5-6) 1.
Page 606 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-6 Components Behind the Center Front Cover and their Functions Figure Reference Reference Component Function Designator A.5-6, 1 ND1 filter positioning knob ND1 is short for a neutral density 1 filter. This filter is already located in the sensing area.
Page 607 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-6 Components Behind the Center Front Cover and their Functions (Continued) Figure Reference Reference Component Function Designator A.5-6, 6 645 DL light filter Dichroic long pass filter used to pass 655 nm to 725 nm fluorescent emissions while reflecting 607 nm to 630 nm wavelengths.
Page 608 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Components Accessed with the Filter Shield Removed Figure A.5-7 Inside View of the Cytometer with the Filter Shield Removed (See Table A.5-7) Front view with filter shield removed 1. Side scatter diode 4.
Page 609 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-7 Components Behind the Filter Shield and their Functions Figure Reference Reference Component Function Designator A.5-7, 1 Side scatter diode Also referred to as SS diode, SS sensor, or SS detector. Collects the laser light that is scattered at about a 90°...
Page 610 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-7 Components Behind the Filter Shield and their Functions (Continued) Figure Reference Reference Component Function Designator A.5-7, 6 Forward scatter detector Also referred to as FS detector, FS sensor or forward scatter sensor.
Page 611 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Components Inside the Reagent Drawer Figure A.5-8 View of an Open Reagent Drawer (See Table A.5-8) 1. VL20 2. Quick-connect 3. Sheath liquid filter 4. Sheath container 5. Cleaning agent container 7029208F A.5-27 PN 4237029F...
Page 612 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-8 Components Inside the Reagent Drawer and their Functions Figure Reference Reference Component Function Designator A.5-8, 1 VL20 Commonly referred to as the sheath filter purge valve. PV 20 inside BOTTLE Normally-closed pinch valve that is manually pushed to DRAWER activate a purge of the sheath filter to remove bubbles.
Page 613 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Rear Panel Components Figure A.5-9 Rear Panel Components, Exterior View (See Table A.5-9) 1. Fans 2. FLOWCELL WASTE quick-connect 3. WASTE quick-connect 4. VACUUM quick-connect 5. PRESSURE quick-connect 6. P13 7. P80 8.
Page 614 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-9 Components Attached to the Rear Panel and their Functions, Exterior View Figure Reference Reference Component Function Designator A.5-9, 1 Fans When power to the Cytometer is turned on, these fans provide a constant flow of room air into the Cytometer to keep the electronic components inside the Cytometer from overheating.
Page 615 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-9 Components Attached to the Rear Panel and their Functions, Exterior View (Continued) Figure Reference Reference Component Function Designator A.5-9, 10 Fiber Optics Interface card Circuit card converts the digital data received from the Cyto Transputer card to optical data then sends that optical data to the Opto Transprocessor EXMEM or Opto Transprocessor EXMEM II card inside the Workstation...
Page 616 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Manual Sample Station Components, Cytometer with MCL Option Figure A.5-10 XL-MCL Manual Sample Station Components (See Table A.5-10) Rear view 1. SHEATH LOW level sense indicator 8. CLEANSE push button 2. WASTE FULL level sense indicator 9.
Page 617 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-10 XL-MCL Manual Sample Station Components and their Functions Figure Reference Reference Component Function Designator A.5-10, 1 SHEATH LOW level sense Red symbol when the sheath fluid is low. indicator During sample analysis, the operator has 5 minutes to finish analyzing the current sample after the indicator starts flashing red.
Page 618 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-10 XL-MCL Manual Sample Station Components and their Functions (Continued) Figure Reference Reference Component Function Designator A.5-10, 7 PRIME push-button Micro contact switch used to initiate the Prime cycle. Press to flush the flow cell with sheath fluid. Data acquisition pauses until the prime cycle is done.
Page 619 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-10 XL-MCL Manual Sample Station Components and their Functions (Continued) Figure Reference Reference Component Function Designator A.5-10, 13 Manual stage air cylinder Solenoid-actuated / spring-return air cylinder used to raise inside CL 1 or lower the manual sample stage.
Page 620 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Manual Sample Station Components, Cytometer without MCL Option Figure A.5-11 XL Manual Sample Station Components (See Table A.5-11) Rear view 1. Front Panel LED and Switch Input card 2. SHEATH LOW / WASTE FULL / CLEANSE LOW level sense indicator 3.
Page 621 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-11 XL Manual Sample Station Components and their Functions Figure Reference Reference Component Function Designator A.5-11, 1 Front Panel LED and Switch Used only on instruments without the MCL option, this Input card circuit card contains the microswitch membrane switches used to initiate the RUN, PRIME, or CLEANSE functions.
Page 622 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-11 XL Manual Sample Station Components and their Functions (Continued) Figure Reference Reference Component Function Designator A.5-11, 4 PRIME push-button Micro contact switch used to initiate the Prime cycle. Press to flush the flow cell with sheath fluid. Data acquisition pauses until the prime cycle is done.
Page 623 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Segmenting Valve Components Figure A.5-12 Segmenting Valve Components (See Table A.5-12) Front view 7029211F 1. PEEK tubing to manual sample probe 6. PEEK tubing to flow cell 2. PEEK tubing to MCL sample probe 7.
Page 624 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-12 Segmenting Valve Components and Their Functions Figure Reference Reference Component Function Designator A.5-12, 1 Peek tubing to MCL Provides a pathway for sample flow from the MCL sample inside probe to the segmenting valve rear pad. PROBE NORMAL block on...
Page 625 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-12 Segmenting Valve Components and Their Functions (Continued) Figure Reference Reference Component Function Designator A.5-12, 9 Air Cylinder for rear Double action air cylinder controlled by 30 psi provided by inside CYL 4 segmenting valve pad either VL17 or VL20.
Page 626 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Components on the MCL Option Carousel Components Figure A.5-13 Carousel Components (See Table A.5-13) 1. MCL door 2. MCL probe housing 3. MCL carousel pushbutton 4. MCL base 5. Carousel hub 6. Interlock switch 7.
Page 627 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-13 Carousel Components and Their Functions Figure Reference Reference Component Function Designator A.5-13, 1 MCL door Exterior cover also referred to as the MCL lid or MCL upper lid and may also be referred to as MCL cover or MCL upper cover.
Page 628 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS MCL Components Accessible with Covers Removed Figure A.5-14 MCL Components Accessible with Covers Removed (See Table A.5-14) Rear view Behind MCL Vertical Plate 1. Probe sensor and flag 9. Motor Filter card (EMC version) 2.
Page 629 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-14 MCL Components Accessible with Covers Removed and Their Functions Figure Reference Reference Component Function Designator A.5-14, 1 Probe sensor and flag Used to monitor the position of the MCL sample probe. A.5-14, 2 MCL sample probe Provides a pathway for the pressurized sample (in the test...
Page 630 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-14 MCL Components Accessible with Covers Removed and Their Functions (Continued) Figure Reference Reference Component Function Designator A.5-14, 15 MCL tube rotator assembly Double action air cylinder controlled by 30 psi and venting used to move the finger in and out to rotate the sample tube for reading the bar-code label and guides the tube as it is being lifted to the MCL sample head.
Page 631 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Components in the Left Side of the Cytometer The main assembly located in the left side of the Cytometer is the lower pneumatics drawer. To access the lower pneumatics drawer, the left side cover must first be removed. If the MCL option is installed, access is more time consuming because the entire MCL option must be removed to access the lower pneumatics drawer.
Page 632 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Figure A.5-15 Lower Pneumatics Drawer Components (See Table A.5-15) 1. Solenoid Power 19. VL7 Mounting plate Distribution card 20. VL8 for laser 2. Solenoid manifold 21. VL9 cooling fans 3. QD10 22. VL10 4.
Page 633 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-15 Components in the Lower Pneumatics Drawer and Their Functions Figure Reference Reference Component Function Designator A.5-15, 1 Solenoid Power Distribution Circuit card that provides the 24 Vdc needed to operate the Refer to card various solenoid valves.
Page 634 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-15 Components in the Lower Pneumatics Drawer and Their Functions (Continued) Figure Reference Reference Component Function Designator A.5-15, 8 VL17 One of two solenoids that provide the 30 psi needed to VL 17 inside operate the double action air cylinder (CYL 4) that controls LOWER...
Page 635 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-15 Components in the Lower Pneumatics Drawer and Their Functions (Continued) Figure Reference Reference Component Function Designator A.5-15, 13 Controls the flow of cleaning agent to the flow cell. VL 1 inside LOWER Type of Valve Normally closed pinch valve controlled by an PNEUMATICS...
Page 636 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-15 Components in the Lower Pneumatics Drawer and Their Functions (Continued) Figure Reference Reference Component Function Designator A.5-15, 20 Controls pushing sheath for Stop on Volume. VL 8 inside LOWER Type of Valve Normally closed pinch valve controlled by an PNEUMATICS electro-pneumatic valve (solenoid/pilot actuator DRAWER...
Page 637 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-15 Components in the Lower Pneumatics Drawer and Their Functions (Continued) Figure Reference Reference Component Function Designator A.5-15, 26 12 insert fitting plate Plate contains a captive knurl knob with fitting inserts at QD 76, QD 77, QD76, QD77, QD78, QD79, QD81, QD82, QD83, QD85,...
Page 638 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-15 Components in the Lower Pneumatics Drawer and Their Functions (Continued) Figure Reference Reference Component Function Designator A.5-15, 30 Waste chamber sensor Often referred to as the eyeball sensor. SN 12 inside the Optical sensor monitors the level of sample waste from the LOWER...
Page 639 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Front Panel Display Components Figure A.5-16 Front Panel Display Components (See Table A.5-16) 1. FS bar graph display 2. SS bar graph display 3. AUX bar graph display 4. FL1 bar graph display 5.
Page 640 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-16 Front Panel Display Components and Their Functions Figure Reference Reference Component Function Designator A.5-16, 1 FS bar graph display Forward scatter signal amplitude indicator. The bar graph shows the intensity of the signal that particles generate during sample analysis.
Page 641 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-16 Front Panel Display Components and Their Functions (Continued) Figure Reference Reference Component Function Designator A.5-16, 6 FL3 bar graph display Fluorescence 3 signal amplitude indicator. The bar graph shows the intensity of the signal that particles generate during sample analysis.
Page 642 QUICK REFERENCE INFORMATION CYTOMETER COMPONENT LOCATIONS AND FUNCTIONS Table A.5-16 Front Panel Display Components and Their Functions (Continued) Figure Reference Reference Component Function Designator A.5-16, 9 SAMPLE FLOW selection Sample flow rate indicators. (LOW / MED / HIGH) When, at the Workstation, the operator chooses a flow rate to control the speed of the sample delivery to the flow cell, the corresponding indicator (LOW / MED / HIGH) glows green at the Cytometer.
Page 643 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Overview The Power Supply module provides and monitors the main electronic and pneumatic power (vacuum and pressure) to the Cytometer. This section identifies the main components and/or assemblies in the Power Supply module, briefly describing their functions and showing their locations.
Page 644 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Power Supply Module Anchor Illustration Figure A.6-1 Power Supply Module, Anchor Illustration for Locating Figure Reference Components A Components behind the front door, Figure A.6-2. B Components in the left-side compartment, Figure A.6-3 C Components in the right-side compartment,...
Page 645 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Table A.6-1 Power Supply Module, Component and Assembly Accessibility Figure Reference Main Component or Assembly that is Accessible To Access Component or Assembly A.6-1, A Various pneumatic components including: Open the front door. r Vacuum and water traps r System pressure and system vacuum gauges r System pressure adjust knob...
Page 646 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Components Located Behind the Front Door of the Power Supply Module Figure A.6-2 View of the Power Supply Module with the Front Door Open (See Table A.6-2) FILTER WATER TRAP TRAP SYSTEM POWER FILTER...
Page 647: Module
QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Table A.6-2 Components behind the Front Door of the Power Supply Module Figure Reference Reference Component Function Designator A.6-2, 1 Argon laser power supply Provides control and ac voltages to the Argon laser head. A.6-2, 2 Vacuum trap Safety device to prevent liquid from entering the vacuum...
Page 648 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Table A.6-2 Components behind the Front Door of the Power Supply Module (Continued) Figure Reference Reference Component Function Designator A.6-2, 6 SYSTEM POWER -15 V supply Indicates the presence or absence of -15 Vdc to the indicator system.
Page 649 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Table A.6-2 Components behind the Front Door of the Power Supply Module (Continued) Figure Reference Reference Component Function Designator A.6-2, 10 MCL POWER -12 V supply Indicates the presence or absence of -12 Vdc to the MCL. indicator When -12 Vdc is available for use by the MCL, the MCL POWER -12 V LED is lighted.
Page 650 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Table A.6-2 Components behind the Front Door of the Power Supply Module (Continued) Figure Reference Reference Component Function Designator A.6-2, 16 Vacuum filter Hydrophobic gas filter removes any particles or moisture FL 2 inside in the vacuum line before it reaches the system vacuum POWER...
Page 651 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Components in the Left Side of the Power Supply Module Figure A.6-3 Left Side View of the Power Supply Module with Cover Removed (See Table A.6-3) P-73 SN13 CB-1 1. Argon laser power supply 6.
Page 652 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Table A.6-3 Components in the Left Side of the Power Supply Module Figure Reference Reference Component Function Designator A.6-3, 1 Argon laser power supply Provides control and ac voltages to the Argon laser head. A.6-3, 2 Compressor Dual-head compressor/vacuum pump.
Page 653 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Table A.6-3 Components in the Left Side of the Power Supply Module (Continued) Figure Reference Reference Component Function Designator A.6-3, 6 Transient Voltage Suppressor 2 Also referred to as the Transient Absorber EMC card, this card small circuit card is mounted to the ac line filter bracket.
Page 654 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Components in the Right Side of the Power Supply Module Figure A.6-4 Right Side View of the Power Supply Module with Cover Removed (See Table A.6-4) 7029180F 5. ±15 Vdc system power supply 1.
Page 655 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Table A.6-4 Components in the Right Side of the Power Supply Module Figure Reference Reference Component Function Designator A.6-4, 1 Interlock switch Safety interlock to ensure the three-sided cover is covering the components inside the Power Supply module when the power is on.
Page 656 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Components on the Rear Panel of the Power Supply Module Figure A.6-5 Rear View of the Power Supply Module (See Table A.6-5) 1. MCL connector 13. COMP ON indicator lamp 2.
Page 657 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Table A.6-5 Components on the Rear Panel of the Power Supply Module Figure Reference Reference Component Function Designator A.6-5, 1 MCL connector Provides the external interface from the Power Supply module to the MCL assembly.
Page 658 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Table A.6-5 Components on the Rear Panel of the Power Supply Module (Continued) Figure Reference Reference Component Function Designator A.6-5, 10 MCL 5 VOLTS circuit breaker Protects the MCL if a short occurs in the MCL +5 Vdc, +12 Vdc, or -12 Vdc supply circuitry.
Page 659 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS Table A.6-5 Components on the Rear Panel of the Power Supply Module (Continued) Figure Reference Reference Component Function Designator A.6-5, 19 WASTE quick-connect Provides a pathway to the waste container for emptying QD 20 (orange) waste that accumulates in the air/water separator (located...
Page 660 QUICK REFERENCE INFORMATION POWER SUPPLY MODULE COMPONENT LOCATIONS AND FUNCTIONS A.6-18 PN 4237029F...
Page 661 QUICK REFERENCE INFORMATION HOT KEYS AVAILABLE WITH SYSTEM II™ SOFTWARE HOT KEYS AVAILABLE WITH SYSTEM II™ SOFTWARE ATTENTION: Hot Keys are not functional when the Acquisition screen is accessed using EXPO32™ software. Acquisition Screen The following Hot Keys are available when the Acquisition screen is displayed using SYSTEM II software.
Page 662 QUICK REFERENCE INFORMATION HOT KEYS AVAILABLE WITH SYSTEM II™ SOFTWARE A.7-2 PN 4237029F...
Page 663 HARDWARE/SOFTWARE CONFIGURATIONS AND REQUIREMENTS FlowCentre™ II MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION The FlowCentre II Multimedia Workstation is a dual-boot system that features both Windows 98 and MS-DOS 6.22. The XL SYSTEM II software is loaded in MS-DOS 6.22 only and is not supported in the Windows 98 operating system. The FlowCentre II computer chassis is now in a tower, not a desktop case.
Page 664 HARDWARE/SOFTWARE CONFIGURATIONS AND REQUIREMENTS FlowCentre™ II MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION DEFAULT Settings for the System BIOS Standard CMOS Setup Floppy Drive A: 1.44 MB 3½ Floppy Drive B: Not Installed Pri Master (Primary Master Disk): Auto Pri Slave (Primary Slave Disk): Not Installed Sec Master (Secondary Master Disk): Auto...
Page 665 HARDWARE/SOFTWARE CONFIGURATIONS AND REQUIREMENTS FlowCentre™ II MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION Advanced Chipset Setup USB Function: Enabled Onboard SCSI-1: Enabled Onboard SCSI-2: Disabled BX Master Latency Timer (Clks): Multi-Trans Timer (Clks): Graphics Aperture Size: 64MB AGP Mlti-Trans Timer (AGP Clks): AGP Low-Priority Timer (AGP Clks): Power Management Setup ACPI Aware O/S: Power Management / APM:...
Page 666 HARDWARE/SOFTWARE CONFIGURATIONS AND REQUIREMENTS FlowCentre™ II MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION PCI / Plug and Play Setup AMI RAID Express Installed: Plug and Play Aware O/S: PCI VGA Palette Snoop: Disabled Allocate IRQ to PCI VGA: Primary Bus Options USB Device Latency: PCI Slot-1 Latency: PCI Slot-2 Latency: PCI Slot-3 Latency:...
Page 667 HARDWARE/SOFTWARE CONFIGURATIONS AND REQUIREMENTS FlowCentre™ II MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION IRQ14: IRQ15: DMA Resource Owner DMA Channel 0: DMA Channel 1: DMA Channel 3: DMA Channel 5: DMA Channel 6: DMA Channel 7: ISA Memory Resource Reserved ISA Card Memory Size: Reserved ISA Card Memory Address: CC000 Peripheral Setup...
Page 668 HARDWARE/SOFTWARE CONFIGURATIONS AND REQUIREMENTS FlowCentre™ II MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION OnBoard Parallel Port IRQ: Disabled Parallel Port Address: Parallel Port Mode: Parallel Port DMA Channel: EPP Version: WINDOWS 98 Configuration from Device Manager Note: To access, select the Windows Start button tt Settings tt Control Panel System tt Device...
Page 669 HARDWARE/SOFTWARE CONFIGURATIONS AND REQUIREMENTS FlowCentre™ II MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION CONFIG.SYS Configuration DEVICE=C:\DOS\HIMEM.SYS DEVICE=C:\DOS\EMM386.EXE NOEMS X=D100-DCFF BUFFERS=63,0 FILES=60 DOS=UMB FCBS=4,0 REM DEVICE=C:\OAKCDROM.SYS /D:MSCD000 DOS=HIGH REM THE NEXT 2 LINES ARE FOR LANTASTIC NETWORKING REM DEVICEHIGH=C:\LANTASTI\PROTMAN.DOS /I:C:LANTASTI REM DEVICEHIGH=C:\LANTASTI\EL90X.DOS REM DEVICEHIGH /L:2,12048 =C:\DOS\SETVER.EXE SHELL=C:\DOS\COMMAND.COM /P /E:1024 REM DEVICE=C:\LANTASTI\PROTMAN.DOS /I:C:\LANTASTI REM DEVICE=C:\LANTASTI\EL90X.DOS...
Page 670 HARDWARE/SOFTWARE CONFIGURATIONS AND REQUIREMENTS FlowCentre™ II MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION AUTOEXEC.BAT Configuration @echo off REM VERIFY ON LH C:\APPS\MOUSE\MOUSE REM THE NEXT LINE IS THE CD-ROM DRIVER REM C:\DOS\MSCDEX.EXE /D:MSCD000 REM THE NEXT LINE ENABLES THE FONT FOR THE ELITE/ALTRA SOFTWARE C:\MATROX\UTIL\VBEXT.EXE PATH=C:\;C:\DOS;C:\XL;C:\APPS\MOUSE;C:\RTSQL REM THE NEXT LINE IS FOR THE LANTASTIC NETWORK OPTION...
Page 671 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS FlowCentre™ MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION FlowCentre™ MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION The FlowCentre Multimedia Workstation is a dual-boot system that features both Windows 95 and MS-DOS 6.22. XL SYSTEM II software is loaded in MS-DOS 6.22 only and is not supported in the Windows 95 operating system.
Page 672 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS FlowCentre™ MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION Installed Drives 32X Internal IDE CD-ROM drive 3.2 Gigabyte IDE hard drive, (mode 4) 1.44 MB floppy drive, 3.5-inch Preloaded Software Windows 95 Operating System and MS-DOS 6.22 Operating System, with dual-boot menu XL SYSTEM II version 3.0 Adaptec EZ-SCSI version 4.x (SCSI Host Adapter software are loaded in C:\SCSI.) Matrox Millenium PowerDesk (Software for Video Display Adapter.)
Page 673 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS FlowCentre™ MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION AMI WIN BIOS Configuration Standard Setup Pri Master (Primary Master Disk): Auto Pri Slave (Primary Slave Disk): Not Installed Sec Master (Secondary Master Disk): Not Installed Sec Slave (Secondary Slave Disk): Not Installed Floppy A: Drive: 1.44 MB 3½...
Page 674 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS FlowCentre™ MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION Shadow CC00, 16K: Disabled Shadow D000, 16K: Disabled Shadow D400, 16K: Disabled Shadow D800, 16K: Disabled Shadow DC00, 16K: Disabled Chipset Setup Memory Hole: Disabled DRAM ECC Mode: Enabled USB Function: Disabled USB Keyboard / Mouse Legacy Support: Disabled Power Mgmt (Power Management) Setup Power Management / APM:...
Page 675 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS FlowCentre™ MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION PCI / PnP Setup PCI VGA Palette Snoop: Disabled PCI Slot-1 Latency Timer: PCI Slot-2 Latency Timer: PCI Slot-3 Latency Timer: Empty Slot PCI Slot-4 Latency Timer: USB Device Latency Timer: Disabled USB Device IRQ Preference: Disabled PCI Slot-1 IRQ Preference: Auto...
Page 676 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS FlowCentre™ MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION Peripheral Setup OnBoard Floppy Controller: Enabled OnBoard Primary/Secondary IDE: Primary OnBoard IDE BusMaster: Enabled IDE Bus Mastering: Enabled OnBoard Primary Prefetch: Disabled OnBoard Secondary Prefetch: OffBoard PCI/ISA IDE Card: OffBoard Primary/Secondary: OffBoard PCI IDE Primary IRQ: OffBoard PCI IDE Secondary IRQ: Serial Port1 IRQ: Disabled...
Page 677 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS FlowCentre™ MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION WINDOWS 95 Configuration from Device Manager Note: To access, select the Windows Start button tt Settings tt Control Panel System tt Device Manager Control Panel Setup, Device Manager, Computer IRQ 00: System Timer IRQ 01: Std 101/102 Key Keyboard IRQ 02:...
Page 678 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS FlowCentre™ MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION CONFIG.SYS Configuration DEVICE=C:\DOS\HIMEM.SYS DEVICE=C:\DOS\EMM386.EXE NOEMS X=CA00-CAFF BUFFERS=63,0 FILES=60 DOS=UMB LASTDRIVE=Z FCBS=4,0 REM DEVICE=C:\DOS\SETVER.EXE DOS=HIGH SHELL=C:\DOS\COMMAND.COM /P /E:1024 REM THE NEXT LINE ACTIVATES THE INTERNAL CD-ROM DRIVE REM DEVICE=C:\SONY_CD\ATAPI_CD.SYS /D:MSCD000 /Q /I:0 REM DEVICEHIGH /L:1,40800 =C:\OAKCDROM.SYS /D:MSCD000 REM THE NEXT 2 LINES ARE FOR THE LANTASTIC NETWORK REM DEVICE=C:\LANTASTI\PROTMAN.DOS /I:C\LANTASTI REM DEVICE=C:\LANTASTI\EL90X.DOS...
Page 679 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS FlowCentre™ MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION AUTOEXEC.BAT Configuration @ECHO OFF REM C:\DOS\SMARTDRV.EXE /X PROMPT $P$G SET TEMP=C:\DOS PATH=C:\;C:\XL;C:\DOS;C:\RTSQL REM THE NEXT LINE INSTALLS THE MOUSE DRIVER, V11.00 LH /L:0 C:\APPS\MOUSE\MOUSE REM LH /L:1,27952 C:\DOS\MSCDEX.EXE /D:MSCD000 REM THE NEXT LINE IS FOR THE LANTASTIC NETWORK OPTION REM call C:\LANTASTI\STARTNET.BAT REM THE STARTNET.BAT FILE REM C:\DOS\MSCDEX.EXE /D:MSCD000 /M:12 /V...
Page 680 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS FlowCentre™ MULTIMEDIA WORKSTATION COMPUTER CONFIGURATION B.2-10 PN 4237029F...
Page 681 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS ® ® INTEL PENTIUM 166 PROCESSOR CONFIGURATION INTEL PENTIUM 166 PROCESSOR CONFIGURATION ® ® The INTEL Pentium 166 processor contains an AMI ATLAS PCI III Motherboard with: 166 MHz INTEL Pentium processor 512 K pipeline burst cache 16 MB system parity RAM (2 to 8 MB SIMMS at 70 ns RAS), expandable to 256 MB memory 3 PCI, 3 ISA slots and 1 shared PCI/ISA slot (PCI bus conforms to the PCI 2.1 Specification)
Page 682 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS ® ® INTEL PENTIUM 166 PROCESSOR CONFIGURATION AMI WIN BIOS Configuration Standard Setup Floppy A: Drive: 1.44 MB 3½ Floppy B: Drive: Not Installed Pri Master (Primary Master Disk): Auto LBA/LARGE Mode: Block Mode: 32-Bit Mode PIO Mode: Mode 4 Pri Slave (Primary Slave Disk): Not Installed...
Page 683 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS ® ® INTEL PENTIUM 166 PROCESSOR CONFIGURATION Caching Controller: Present Video Shadow C000, 32K: Cached Shadow C800, 16K: Disabled Shadow CC00, 16K: Disabled Shadow D000, 16K: Disabled Shadow D400, 16K: Disabled Shadow D800, 16K: Disabled Shadow DC00, 16K: Disabled Chipset Setup Memory Hole:...
Page 684 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS ® ® INTEL PENTIUM 166 PROCESSOR CONFIGURATION PCI / PnP Setup PCI VGA Palette Snoop: Disabled PCI Slot-1 Latency Timer: PCI Slot-2 Latency Timer: PCI Slot-3 Latency Timer: Empty Slot PCI Slot-4 Latency Timer: Empty Slot USB Device Latency Timer: No Selection USB Device IRQ Preference: No Selection...
Page 685 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS ® ® INTEL PENTIUM 166 PROCESSOR CONFIGURATION Peripheral Setup OnBoard Floppy Controller: Enabled OnBoard Primary/Secondary IDE: No Selection OnBoard IDE BusMaster: No Selection OffBoard PCI/ISA IDE Card: PCI Slot 1 OffBoard Primary/Secondary: Both OffBoard PCI IDE Primary IRQ: Disabled OffBoard PCI IDE Secondary IRQ: Hardwired...
Page 686 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS ® ® INTEL PENTIUM 166 PROCESSOR CONFIGURATION B.3-6 PN 4237029F...
Page 687 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS ® LANtastic NETWORK OPERATING SYSTEM MINIMUM REQUIREMENTS ® LANtastic NETWORK OPERATING SYSTEM MINIMUM REQUIREMENTS The following are the minimum requirements for the LANtastic network operating system (NOS) as provided by Artisoft, Inc. These are for a dedicated network server and do not include support for SYSTEM II software installed on the server.
Page 688 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS ® LANtastic NETWORK OPERATING SYSTEM MINIMUM REQUIREMENTS B.4-2 PN 4237029F...
Page 689 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS ® Sybase SQL ANYWHERE™ MINIMUM REQUIREMENTS ® Sybase SQL ANYWHERE™ MINIMUM REQUIREMENTS The following are the minimum requirements for the Sybase SQL Anywhere PC database server software as provided by Sybase, Inc. These are for a dedicated network server and do not include support for SYSTEM II software installed on the server.
Page 690 HARDWARE/SOFTWARE CONFIGURATIONS/REQUIREMENTS ® Sybase SQL ANYWHERE™ MINIMUM REQUIREMENTS B.5-2 PN 4237029F...
Page 691 FIELD WORKSHEETS WORKSHEETS Field Engineer Worksheet Mean Mean Mean Mean Mean Run # HP CV Channel HP CV Channel HP CV Channel HP CV Channel HP CV Channel Add lines 0 through 9 to get TOTALS Totals PN 4237029F C.1-1...
Page 692 FIELD WORKSHEETS WORKSHEETS Network Configuration Worksheet Serial Number _______________________________________________________________ Network Node Name _________________________________________________________ Server Attached To ___________________________________________________________ CPU Type _____________________________ RAM Size ____________________________ PRINTER TYPE _____________________________________________________________ LPT1 ___________________________________________________________________ LPT2 ___________________________________________________________________ DISK DRIVES A: ____________________________ B: ____________________________ C: ____________________________ D: ____________________________ E: ____________________________ MS DOS Version _________________________________ Cytometer Software Version ________________________...
Page 693 EXAMPLES OF SETUP FILES CLIENT FLOW CYTOMETER Config.sys DEVICE=C:\DOS\HIMEM.SYS DOS=HIGH DEVICE=C:\DOS\SETVER.EXE FILES=60 BUFFERS=30 LASTDRIVE=Z DEVICE=C:\APPS\MOUSE\MOUSE.SYS DEVICE=C:\LAN\PROTMAN.DOS /I:C:\LAN DEVICE=C:\LAN\ELNK3.DOS Autoexec.bat SET CGIPATH=C:\XL\GSS @ECHO OFF PROMPT $p$g PATH C:\DOS SET TEMP=C:\DOS call C:\LAN\STARTNET.BAT kwcaf 500,100 cd \XL Startnet.bat @echo off cd C:\LAN SET LAN_CFG=C:\LAN rem If LANtastic is disabled, skip everything.
Page 694 EXAMPLES OF SETUP FILES CLIENT FLOW CYTOMETER rem Otherwise set up connections specified during install. NET LPT TIMEOUT 10 GOTO :CONNECT_DONE :CONNECT @echo Setting up LANtastic connections from CONNECT.BAT rem Build CONNECT.BAT like this: "NET SHOW/BATCH > C:\LAN\CONNECT.BAT" rem (or run the batch file SETNET.BAT) call CONNECT.BAT :CONNECT_DONE NET POSTBOX...
Page 695 EXAMPLES OF SETUP FILES FILE SERVER FILE SERVER Config.sys DEVICE=C:\CORELDRV\ASPIDRV.SYS DEVICE=C:\DOS\SETVER.EXE DEVICE=C:\DOS\HIMEM.SYS DOS=HIGH FILES=100 BUFFERS=30 STACKS=9,256 LASTDRIVE=Z DEVICEHIGH=C:\CORELDRV\UNI_ASP .SYS DEVICE=C:\LANTASTI\PROTMAN.DOS /I:C:\LANTASTI DEVICE=C:\LANTASTI\ELNK3.DOS rem DEVICE=C:\CORELDRV\CUNI_ASP .SYS Autoexec.bat C:\DOS\SMARTDRV.EXE /X @ECHO OFF PROMPT $p$g PATH c:\wsql40\win;C:\WINDOWS;C:\DOS SET MSINPUT=C:\MSINPUT C:\MSINPUT\MOUSE\MOUSE.EXE /Q SET TEMP=C:\DOS call C:\LANTASTI\STARTNET.BAT cd c: SET PATH=%PATH%;C:\CORELDRV...
Page 696 EXAMPLES OF SETUP FILES FILE SERVER @echo ===== Begin LANtastic configuration ===== PATH C:\LANTASTI;C:\LANTASTI\NW;%PATH% SET LAN_DIR=C:\LANTASTI.NET SET NWDBPATH=C:\LANTASTI\NW LOADHIGH AI-NDIS BIND_TO=ELNK3_NIF AILANBIO @STARTNET.CFG REDIR RESEARCH_LAB @STARTNET.CFG net use S: \\jeeves\e-drive IF EXIST NOSHARE GOTO :NOSHARE SERVER C:\LANTASTI.NET @STARTNET.CFG NET LOGIN \\RESEARCH_LAB GOTO :CONTINUE :NOSHARE @echo LANtastic server was installed but turned off.
Page 697 NETWORK PROTOCOL SPECIFICATIONS 802.3 Table E.1-1 802.3 Specifications Item 10BASE5 10BASE2 10BASE-T Data rate (MBPS) Signaling type Baseband/Manchester Baseband/Manchester Baseband/Manchester Maximum segment length (meters) Media COAX (thick) COAX (thin) 50 ohm 50 ohm RG-8, RG-11 RG-58 Topology Star Requires transceivers Requires terminators Requires a hub device Number of nodes per segment/length...
Page 698 NETWORK PROTOCOL SPECIFICATIONS 802.3 E.1-2 PN 4237029F...
Page 699 BAR-CODE SPECIFICATIONS BAR-CODE LABELS A bar code consists of black lines (bars) and white lines (spaces), which are called elements. There are narrow elements (NE) and wide elements (WE). The bar-code symbology determines their arrangement. IMPORTANT Possible incorrect sample identification. When sample tube bar-code labels do not follow the specification in this section, incorrect sample identification can occur.
Page 700 Codabar, Code 39 and Interleaved 2-of-5 labels. In one study, the use of check sum digits detected 97% of misread errors. Beckman Coulter strongly recommends the use of bar-code check sums to provide automatic checks for read accuracy. Use check sums to provide protection against occasional misread errors caused by problems such as damaged or misapplied labels.
Page 701 BAR-CODE SPECIFICATIONS MCL BAR-CODE SCANNER MCL BAR-CODE SCANNER Types of Scanners The XL-MCL flow cytometer uses a visible laser-type scanner containing a Class II laser, operating at 670 nm, with a maximum power output of 1 mW. The MCL bar-code scanner is used on the XL-MCL flow cytometer only.
Page 702 BAR-CODE SPECIFICATIONS MCL BAR-CODE SCANNER For an Instrument with an Original EEPROM Installed If the XL-MCL flow cytometer has the original EEPROM (OEM part number 35-213064-10) installed on the Bar-Code Decoder card, use the following AUX port settings: Bits per second: 1200 Parity: Data bits:...
Page 703 BAR-CODE SPECIFICATIONS MCL BAR-CODE SCANNER Symbologies IMPORTANT Possible incorrect identification of sample tubes. If sample tube bar-code labels use FNC1, FNC4, and FS (hexadecimal 1C) characters in the bar-code information, incorrect identification of sample tubes can occur. To prevent incorrect identification of sample tubes, do not use FNC1, FNC4, and FS (hexadecimal 1C) characters in your bar-code information.
Page 704 BAR-CODE SPECIFICATIONS MCL BAR-CODE SCANNER F.2-4 PN 4237029F...
Page 705 BAR-CODE SPECIFICATIONS HAND-HELD BAR-CODE SCANNER OPTION HAND-HELD BAR-CODE SCANNER OPTION Types of Scanners The hand-held bar-code scanner uses a visible laser-type reader containing a Class II laser, operating at 670 nm, with a maximum power output of 1 mW. This optional scanner may be attached to an XL or XL-MCL flow cytometer.
Page 706 BAR-CODE SPECIFICATIONS HAND-HELD BAR-CODE SCANNER OPTION General Parameters Table F.3-2 Hand-Held Bar-Code Scanner - General Parameters Item Code* Function AUTOSENSE OPERATION Disabled WEDGE MODE Enabled BEEPER OPERATION Beeper ON; volume LOUD INTERCHARACTER DELAY No intercharacter delay PREFIX None SUFFIX Suffix CR TERMINAL ID Disabled CODE IDENTIFIER...
Page 707 BAR-CODE SPECIFICATIONS HAND-HELD BAR-CODE SCANNER OPTION Table F.3-3 Hand-Held Bar-Code Scanner - Symbologies (Continued) Item Code* Function CODE I-2 0F 5 Enable I-2 of 5 with check digit CODE I-2 OF 5 MINIMUM LENGTH Minimum length = 14 CODE I-2 OF 5 MAXIMUM LENGTH Maximum length = 14 CODE I-2 OF 5 START/STOP CHAR.
Page 708 BAR-CODE SPECIFICATIONS HAND-HELD BAR-CODE SCANNER OPTION F.3-4 PN 4237029F...
Page 709 BAR-CODE SPECIFICATIONS BAR-CODE PRINTER BAR-CODE PRINTER Table F.4-1 Bar-Code Printer - DIP Switch Settings Switch Setting Position Function Right Right Sets BAUD rate to 9600 Right Left Sets Data Bit length to 8 Left Sets Parity to Disabled Left Right Sets X ON/OFF Flow Control Right Sets no error detection...
Page 710 BAR-CODE SPECIFICATIONS BAR-CODE PRINTER F.4-2 PN 4237029F...
Page 711 CONTENTS ABBREVIATIONS, ABBREVIATIONS-1 GLOSSARY, GLOSSARY-1 PN 4237029F 10-i...
Page 712 CONTENTS 10-ii PN 4237029F...
Page 713 ABBREVIATIONS The following list is a composite of the abbreviations, acronyms and reference designators used in this manual. When the same abbreviation (or reference designator) is used for more than one word (or type of component), all meanings relevant to this manual are included. SYMBOLS >...
Page 714 ABBREVIATIONS DOS - disk operating system HV - high voltage DPI - dots per inch Hz - hertz DRAM - direct random access memory DVM - digital volt meter i.d. - internal diameter I/O - input/output E - receptacle connector ID - identification ECC - electronic cycle check IDE - integrated drive electronics...
Page 715 ABBREVIATIONS PMI - preventative maintenance inspection M - Mega PMT - photo-multiplier tube MB - megabyte PN - part number MBPS - megabytes per second PNP - plug and play MCL - multi-tube carousel loader pot - potentiometer MHz - mega hertz PPM - pages per minute MIMD - multiple instruction multiple data PROM - programmable read-only memory...
Page 716 ABBREVIATIONS U - integrated circuit package UL - Underwriter’ s Laboratory UPC - universal product code USB - universal serial bus V - volts Vac - volts alternating current Vdc - volts direct current VDI - video display interface VGA - video graphics array WAN - wide area network WE - wide elements X - receptacle connector...
Page 717 GLOSSARY This glossary is a collection of specialized terms, with their meanings. If a term has more than one meaning, all meanings relevant to this manual are included. µL - Microliter, a unit of volumetric measurement equal to 10 liter. µm - Micron or micrometer, a unit of linear measurement equal to 10 meter.
Page 718 GLOSSARY attenuation - A variable gain adjustment used typically with photocells. autoexec.bat - Tells the computer how to start up and typically is used to execute the most commonly used program. Echo on displays the command currently executed. Verify on checks to see if data has been correctly written to disk and returns error message if not.
Page 719 GLOSSARY coefficient of variation (CV) An expression, in percent (%), of the data spread (variation) as related to the mean value. The standard formula for calculation: × ------------- - Mean A measure of the variability in signal intensity that is generated as particles pass repeatedly through the laser beam.
Page 720 GLOSSARY defaults - Original settings for the instrument. An operator may change these settings to customize the instrument for their laboratory. deionized water - Water freed of salt and some organisms by an ion-exchange process. This water can be used interchangeably with distilled water in procedures. Also referred to as DI dichroic - A filter placed at a 45 degree angle to the incident light used to separate such light into two color bands, one reflected off and the other passing through the filter.
Page 721 This acquisition, analysis, and Cytometer control software product with an Advanced Digital Compensation feature was developed by Applied Cytometry System (ACS) exclusively for Beckman Coulter. The software may also be used for data analysis on a stand alone PC that has Windows 95 or higher as its operating system.
Page 722 GLOSSARY focal point - A point at which the rays of light converge or from which they diverge; for example. light rays as they pass through a lens may converge on a point referred to as the focal point of the lens. format - To lay out a disk in specific tracks, sectors, and blocks so that information can be systematically stored and retrieved to and from the disk.
Page 723 GLOSSARY histogram, light-scatter - A two dimensional graphic presentation of multidimensional accumulated data. Usually the X-axis is set to Side Scatter and the Y-axis is set to Forward Scatter. hot keys - A shortcut for changing screens. Instead of using the menu bar to change screens, you can press and hold down ALT while pressing a certain letter key.
Page 724 GLOSSARY listmode - The digitized pulses for each parameter for each event; e.g. the raw data. logarithmic amplification - A method of increasing the gain and dynamic range of a signal. A larger gain is applied to a sensor’ s smaller signals than to the sensor’ s larger signals. Also see gain.
Page 725 GLOSSARY optical filters - Mediums, such as glass, that separate fluorescent light by wavelength, which is measured in nanometers (nm). Also see BK, BP, and DL filters. panel - A set of protocols to be used in sequence. PE - Phycoerythrin, a dye exciting at 488 nm and emitting in the yellow end of the spectrum primarily used in cell surface marker applications.
Page 726 GLOSSARY prism - to separate out: Triangular glass used to separate multicolored light into the component colors or redirect a single color light to a different location. A circuit which separates multiple cell surface marker measurements into the possible marker phenotypes. Phenotype parameter for multicolor analysis.
Page 727 GLOSSARY scopegram - Same as scattergram. scroll bar - The bar with an up and down arrow on the left of a window. The bar’ s arrows let you move (scroll) the window’ s content up or down so that you can see other parts of it. For example, the scroll bar in the Protocol Select window lets you scroll through the entire list of protocol names.
Page 728 GLOSSARY Standard Deviation - A measure of deviation from the mean. For example, a measure of the range of channel deviation within a measurement. SD, the acronym for standard deviation, may be used interchangeably. ∑ – -------------------------- - – Texas red - A dye exciting at 595 nm and fluorescing in the orange end of the spectrum usually used with the cell surface marker application.
Page 729 675 BP light filter locations in the Cytometer, A.2-2 function, A.5-22 offset adjustment, 4.15-1 802.3 network standard part number, 8.1-23, 8.2-25 Beckman Coulter supported, 2.5-5 PMT1 card function, A.5-10 specifications, 2.5-5 PMT2 card function, A.5-10 specifications, table, E.1-1 PMT3 card function, A.5-10...
Page 730 INDEX function by plane, table, 2.2-6 function in Cytometer, 2.2-1 bar-code location in the Cytometer, A.2-3 acceptable, table, F .1-1 rear view, A.2-4 specifications, F .1-1 anchor illustrations Bar-Code Decoder card for Cytometer component locations and component locations, A.2-5 functions, A.5-2 function, A.5-46...
Page 731 8.2-37 Front Panel LED and Switch Input card replacement/alignment, 4.5-1 MCL CPU. See MCL CPU card Beckman Coulter service tool kit MCL Interface. See MCL Interface card part number, 3.3-1, 8.1-31 Motor Filter. See Motor Filter card Beckman Coulter-supported networking Opto Transprocessor EXMEM II.
Page 732 INDEX Power Module Control. See Power Module function in an XL flow cytometer, A.5-38 Control card function in an XL-MCL flow Sensor. See Sensor card cytometer, A.5-34 Serial/Parallel Adapter Interface. See CMOS Serial/Parallel Adapter Interface card settings for Serial/Parallel Adapter Solenoid Power Distribution.
Page 733 INDEX Power Supply module, example, D.1-1 illustration, A.6-12 editing for LANtastic Client/Server located in right-side compartment of the software V6.0 installation, 3.17-2 Power Supply module, table, A.6-13 editing for LANtastic Client/Server located in the Cytometer right-side software V7.0 installation, compartment, illustration, A.5-16 MS-DOS, 3.16-3...
Page 734 INDEX Cyto Transputer card table, A.5-56 block diagram, 2.2-10 function, illustration, 2.2-1 component locations, A.2-7 in initial installation, 3.3-2, 3.3-3 description, 2.2-10 in SVP, 5.1-1 engineering schematic, part lower pneumatics drawer component number, 6.1-1 locations, illustration, A.5-48 function, A.5-8 lower pneumatics drawer components, jumper settings, A.2-7 table,...
Page 735 INDEX locations, table, A.5-17 function in the Cytometer, A.5-30 XL-MCL flow cytometer, illustrated parts Fiber Optic Interface card listing, 8.2-4, 8.2-6, 8.2-8, 8.2-10 component locations, A.2-8 CYTO-TROL control cells description, 2.2-14 part number, 3.2-1, 8.1-7 engineering schematic, part number, 6.1-1 jumper settings, A.2-8 location in the Cytometer,...
Page 736 INDEX FlowCentre II computer location, illustration, 4.5-4 configuration, B.1-1, B.2-1 FS mask alignment FlowCentre II Multimedia Workstation correct/incorrect positioning, part number, 3.10-1 illustration, 4.6-3 procedures for upgrade, 3.10-1 procedure, 4.6-2 FlowCentre II Multimedia Workstation FS sensor. See FS detector upgrade installation keyboard connection, 3.10-2 mouse connection,...
Page 737 INDEX flexible duct components, 8.2-98 installation lifter air cylinder, 8.2-20 4-color PMT upgrade, 3.9-1 lower pneumatics drawer, 8.2-62 EXPO32 ADC software option, 3.19-1 MCL vertical plate components, 8.2-18 EXPO32 software options, 3.19-1 mounting hardware, 8.2-97 LANtastic Client/Server software Power Supply module, left side, 8.2-76 option, 3.15-1...
Page 738 INDEX definition, 2.5-1 components tested, 7.2-8 LANtastic Client/Server software V6.0 description, 7.2-8 installation, LED line editor, 3.17-1 response to failure, 7.2-8 installation, NIC, 3.17-3 laser duct out plenum installation, NIC relationship, 3.17-1 function, A.5-14 installation, procedure, 3.17-1 laser head installation, running LANCHECK, 3.17-3 Argon, installation in laser installation, tools/supplies needed,...
Page 739 INDEX 12 insert fitting plate connections, A.5-53 manuals, customer 5 insert fitting plate connections, A.5-53 part numbers, 1.1-1 component locations, illustration, A.5-48 master parts list components, table, A.5-49 categories, 8.1-1 illustrated parts listing, 8.2-62 common names, 8.1-1 in initial installation, 3.3-12 component names, 8.1-1...
Page 740 INDEX description, 7.2-11 response to failure, 7.2-12 MCL interconnect sensor status, 7.2-12 engineering schematic, part MCL POWER UP Status, Prefinal software number, 6.1-1 description, 7.2-13 MCL Interface card response to failure, 7.2-13 block diagram, 2.2-11 MCL Probe Align, Prefinal software component locations, A.2-12 description,...
Page 741 2.2-2 copy to use, C.1-2 beam shaping, illustration, 2.2-2 networking description, 2.2-2 802.3, 2.5-1 OPTO DMA Test, Prefinal software Beckman Coulter’s implementation, 2.5-1 circuit cards tested, 7.2-19 bridges, 2.5-4 description, 7.2-19 carrier sense multiple access, 2.5-5 response to failure, 7.2-19 collision detection, 2.5-5...
Page 742 INDEX part number (EMC version), 8.1-24 monitors, 8.1-9 testing with Prefinal Service online manual on CD-ROM, 8.1-31 software, A.4-3 optional items, supplies, tools, 8.1-19 Opto Transprocessor EXMEM card Power Module Control block diagram, 2.2-12 card, 8.1-24, 8.2-77 component locations, A.2-13 power supplies, XL flow cytometer, 8.1-16 description,...
Page 743 INDEX number, 6.1-1 adjustment, procedures, 4.20-4 function, A.5-14 -15 Vdc voltage verification and in 4-color PMT upgrade, 3.9-2 adjustment, procedures, 4.20-4 location in the Cytometer, A.2-15 location, illustration, 8.2-89 PMT filters MCL voltage verification and adjustment, locations in 4-color PMT system, procedures, 4.20-6 illustration,...
Page 744 INDEX Power Module Control card flow function, A.5-45 diagram, 2.3-2 procedures power ON/OFF switch location, +15 Vdc power supply voltage verification illustration, 2.3-3 and adjustment, 4.20-4 rear panel, components, table, A.6-15 +24 Vdc power supply voltage verification rear panel, illustration, A.6-14 and adjustment, 4.20-1...
Page 745 INDEX installation, 3.17-2 Prefinal Service software flow cell and beamshaper replacement installation/operation using SYSTEM II and/or alignment, 4.5-1 software, 4.2-1 flow cell replacement and/or preparing instrument for alignment, 4.5-1 shipment, 4.16-1 FlowCentre II Multimedia Workstation re-imaging FlowCentre computers, 4.21-1 upgrade installation, 3.10-1 reverse/shorted ground/neutral FS mask alignment,...
Page 746 INDEX function, A.5-20 cytometer, A.5-33 QD35 function, A.5-28 safety interlock interconnect engineering schematic, part reagent drawer number, 6.1-1 component locations, illustration, A.5-27 safety precautions components, table, A.5-28 biological, 1.2-2 rear panel during segmenting valve cleaning, 4.11-1 component locations, illustration, A.5-29 during troubleshooting, 1.2-7, 4.9-1 components, table,...
Page 747 INDEX segmenting pads installation, verification, 3.13-2 in initial installation, 3.3-13 jumper settings, illustration, 3.13-1 in PEEK tubing replacement, 4.9-3 part number, 3.13-1, 8.1-24 segmenting valve removal, 3.13-1 assembly, illustrated parts listing, 8.2-58 servers cleaning the shaft, 4.11-3 types, 2.5-5 cleaning, preparation, 4.11-1 service and repair procedures cleaning, segmenting pad...
Page 748 INDEX side scatter. See SS at the end of installation, 3.7-6 in 4-color PMT upgrade, 3.9-3 function, A.5-18 in Amp/Signal Conditioner card replacement and/or calibration, 4.15-6 function, A.5-18 in FlowCentre II Multimedia Workstation upgrade installation, 3.10-5 function, A.5-19 in MCL option installation, 3.8-4 in optical alignment, 4.6-4...
Page 749 INDEX description, 7.2-23 procedures, 4.14-1 replacement and/or calibration, tools/supplies needed, 4.14-1 replacement and/or calibration, T805 Test, Prefinal software verification, 4.14-4 circuit cards tested, 7.2-24 replacement and/or calibration, when to description, 7.2-24 perform, 4.14-1 response to failure, 7.2-24 testing with Prefinal Service Temperature Test, Prefinal software software, A.4-1...
Page 750 INDEX circuit card tested, 7.2-25 description, 7.2-25 function, A.5-51 response to failure, 7.2-25 Valve Sequence, Prefinal software function, A.5-51 description, 7.2-26 valves function, A.5-51 part numbers, 8.1-32 function, A.5-52 function, A.5-50 function, A.5-52 function, A.5-51 VME Addr Bus Test, Prefinal software VL10 circuit cards tested, 7.2-26...
Page 751 INDEX definition, 1.1-4 waste chamber function, A.5-54 Waste Chamber Full Test, Prefinal software components tested, 7.2-27 description, 7.2-27 response to failure, 7.2-27 waste chamber sensor function, A.5-54 waste container illustrated parts listing, 8.2-91 waste full indicator how to read, table, 7.1-1 waste manifold function,...
Page 752 INDEX INDEX-24 PN 4237029F...
Page 753 TRADEMARKS The BECKMAN COULTER logo, COULTER, COULTER CLENZ, CYTO-TROL, EPICS, EXPO, Flow-Check, FlowCentre, IsoFlow, SYSTEM II, XL and XL-MCL are trademarks of Beckman Coulter, Inc. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.

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Beckman Coulter COULTER EPICS XL Service Manual

Table of Contents

Warnings and Precautions

Revision Status

Illustrations

Contents

1 Introduction

2 Instrument Description

3 Installation Procedures

4 Service and Repair Procedures

Quick Links

Chapters

Troubleshooting

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