Fluidics System - Beckman Coulter CytoFLEX Series Instructions For Use Manual

Flow cytometers
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System Overview

Fluidics System

Fluidics System
The fluidics system consists of two parts: the Fluid Containers/Cubitainers and the Fluidics module.
The Fluidics module is located on the right side of the Cytometer. You need to open the right-side
cover of the instrument (see
Replacement/Adjustment
helps to transmit the sheath fluid at a stable rate into the flow cell, forming a laminar fluidics system
that ensures that the tested particles go through the detection area sequentially.
NOTE
The 10 L sheath fluid and waste cubitainers are available from Beckman Coulter as an alternative to
the 4 L Fluid Containers (refer to
Cytometers.
the 10 L sheath fluid and waste cubitainers. Refer to
Figure 1.7 Fluid Containers [CytoFLEX]
1. Fluid Container holder. Holds the Fluid Containers.
2. Fluid sensor holder cutout. Holds the sheath fluid harness and the waste harness when removed from
their respective containers to protect the sensors from damage and/or contamination.
3. Sheath fluid harness. Connects to the sheath fluid container; conveys the sheath fluid into the
instrument. The sheath fluid harness includes the a level sensor, sheath fluid tubing, and backflush
tubing. The other end of the harness is connected to the Fluidics module in the Cytometer. When the
sheath fluid container is near empty, a warning notice is transmitted to the instrument and an audible
signal sounds as a warning.
4. Sheath fluid container. 4 L capacity, for holding sheath fluid. Beckman Coulter recommends using
CytoFLEX Sheath Fluid or a similar nonionic sheath fluid to ensure system performance.
NOTE
5. Waste harness. Connects to the waste fluid container; conveys the waste fluid from the instrument to
the waste container. The waste harness includes a level sensor. The other end of the harness is
connected to the Fluidics module in the Cytometer. When the waste fluid container is near full, a warning
notice is transmitted to the instrument and audible signal sounds as a warning.
6. Waste container. 4 L capacity, for holding waste liquids. Attention to biosafety and waste labeling is
required.
NOTE
1-10
Right-Side Cover Removal and Reinstallation
Procedures) to perform maintenance operations. The fluidics system
Figure
Contact us
to order the 10 L Sheath/Waste Line Kit. The CytoFLEX LX is only available with
d
c
b
The sheath fluid container must be on the same level as the Cytometer.
The waste container must be on the same level as the Cytometer.
1.7) provided with your CytoFLEX and CytoFLEX S Flow
Figure
1.8.
e
f
g
Sheath
Sheath
Waste
in
CHAPTER 11,
B49006AH

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CytoflexCytoflex sCytoflex lx

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