Beckman Coulter CytoFLEX Series Instructions For Use Manual page 286

Flow cytometers
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Troubleshooting
Troubleshooting Table
Table 9.1 Troubleshooting (Continued)
Problem
QC aborted due to low
event rate.
9-20
Probable Cause
• The diluted CytoFLEX Daily
QC Fluorosphere or CytoFLEX
IR Daily QC Fluorosphere
concentration is too low.
• The sample probe is clogged.
• The sample line is clogged.
Corrective Action
1. Add 1 drop of CytoFLEX Daily QC
Fluorospheres to the QC solution. Then,
rerun QC.
2. Reload the target value file. Refer to
Importing Lot-Specific Target Values
CHAPTER 4, Instrument Quality Control
and
Standardization. Then, rerun QC.
3. Prepare a new sample of the CytoFLEX
Daily QC Fluorospheres. Then, rerun QC.
4. Clean the sample probe. Refer to
Cleaning the Sample Probe
CHAPTER 10, Cleaning
Procedures. Then,
rerun QC.
5. Run Prime. Refer to
Priming the Flow Cell
in
CHAPTER 11, Replacement/
Adjustment
Procedures. Then, rerun QC.
6. Run Daily Clean. Refer to
CHAPTER 10, Cleaning
Procedures. Then,
rerun QC.
7. Replace the sample probe and sample
peristaltic pump tubing. Refer to
Replacing the Sample Probe and/or the
Sample Peristaltic Pump Tubing
CHAPTER 11, Replacement/Adjustment
Procedures. Then, rerun QC.
8. Is problem persists,
contact
in
in
Daily Clean
in
in
us.
B49006AH

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