Beckman Coulter CytoFLEX S Manuals

Manuals and User Guides for Beckman Coulter CytoFLEX S. We have 2 Beckman Coulter CytoFLEX S manuals available for free PDF download: Instructions For Use Manual

Beckman Coulter CytoFLEX S Instructions For Use Manual (480 pages)

Flow Cytometers

Brand: Beckman Coulter | Category: Laboratory Equipment | Size: 32.19 MB

Revision History
3
Alerts for Danger, Warning, and Caution
9
Safety Notices
9
Safety Precautions
10
Symbol Explanations
11
Table of Contents
13
About this Manual
27
How to Use Your Manual
27
Introduction
27
Overview
27
Conventions Used
29
Graphics
29
Overview
31
Product Description
31
Sample Station
31
System Overview
31
Main Components
32
Main Components [Cytoflex Without Plate Loader]
32
Main Components [Cytoflex LX]
33
Optical Components
34
Optical Filter Mount with Optical Filter
35
Optical Filter Mounts
35
Wavelength Division Multiplexer (WDM)
35
Optical Filter Mount (Top)
36
Optical Filter Mount Labeled with the Band-Pass
36
WDM Optical Filter Mount Color Codes [Cytoflex LX]
37
WDM Optical Filter Mount Color Codes [Cytoflex]
37
[Cytoflex
37
Cytoflex
39
Optical Fiber
39
Fluidics System
40
Fluid Containers/Cubitainers
41
Fluidics Module
42
Fluidics Module View
42
Fluidic Connections
43
Sample Station
44
Sample Tube Holder Positions
45
Plate Loader Components
46
Plate Holder
48
Plate Holder Components
48
Plate Holder Stage
49
System Configuration
50
System Configuration [Cytoflex]
50
System Connections
50
Back Cover Connections
51
Front of Cytometer [Cytoflex Without Plate Loader Shown]
51
System Configuration [Cytoflex LX]
52
System Connections
52
Back Cover Connections
53
Consumables and Supplies
54
Material Safety Data Sheets (SDS/MSDS)
54
Ordering Information
54
Reagents
54
Acoustic Noise Level
55
Dimensions [Cytoflex LX]
55
Dimensions [Cytoflex]
55
Electrical Ratings
55
Installation Category
55
Instrument Specifications
55
Pollution Degree
55
Cytometer
56
Performance Characteristics
58
Performance Characteristics [Cytoflex]
58
Performance Characteristics [Cytoflex LX]
59
Reagent Limitations
59
CHAPTER 2: Using the Cytexpert Software

61
- Overview
  61
- Launching the Software
  61
- Main Software Screen
  61
- Start Page
  62
- Acquisition Screen
  63
- Acquisition Screen Navigation
  64
- Collection [With Plate Loader]
  65
- Test Tubes
  67
- Plot Area
  68
- Status Bar
  69
- Analysis Screen
  69
- Compensation Experiment Screen
  70
- Drawing Controls Toolbar (Top of Screen)
  70
- QC Experiment Screen
  72
- QC Report Screen
  72
- QC Experiment Screen
  73
- QC Screen Navigation
  73
- Software Menu
  74
- Plate Loader
  74
- Software Menu Tree
  74
- Acquisition and Analysis Screen Menu
  75
- QC Software Menu Tree
  75
- User Management
  82
- User Manager (Card View)
  83
- User Manager (Grid View)
  83
- Creating, Deleting, and Modifying Users in User Manager
  84
- Creating a New User in User Manager
  84
- Deleting Users in User Manager
  85
- Modifying Users in User Manager
  85
- Unlocking a User Account
  86
- Resetting a User Passwords
  86
- Changing a User Password
  86
- Role Management
  86
- Role Manager
  87
- Creating, Deleting, and Modifying User Roles in Role Manager
  88
- Creating New User Roles in Role Manager
  88
- Deleting User Roles in Role Manager
  89
- Modifying User Roles in the Role Window
  89
- Account Policies
  90
- User Management Operation Log
  91
- Viewing and Exporting User Logs
  91
- Graphic and Gating Styles
  92
- Plots
  92
- Gates
  93
- Software Settings
  94
- Language Settings
  97
- Setting up Cytexpert Application Programming Interface (API) Test Client
  97
- Daily Startup
  99
- Overview
  99
- Pre-Startup Inspection
  99
- Check Waste and Reagent Levels [4 L Fluid Containers]
  100
- Check Waste and Reagent Levels [10 L Fluid Cubitainers]
  102
- Power Source Inspection
  103
- Workstation Connections Inspection
  103
- Turning on the Instrument
  104
- Logging into the Software
  104
- Logging out of the Software
  107
- Selecting the Proper Sample Injection Mode
  107
- Using Semi-Automatic Injection Mode
  108
- Using the Manual Injection Mode
  109
- Selecting the Plate Loader Sample Injection Mode [With Plate Loader]
  110
- Using Plate Loader Injection Mode
  110
- Running the System Startup Program with the Single Tube Loader
  112
- Running the System Startup Program [With Plate Loader]
  115
- Selecting Experiments from the Start Page
  122
- Initializing the Instrument
  123
- Instrument Quality Control and Standardization
  125
- Overview
  125
- Preparing the QC Sample
  127
- Required Materials
  127
- Preparation Process Cytoflex Daily QC Fluorospheres
  127
- Preparation Process Cytoflex IR Daily QC Fluorospheres
  127
- Preparing the QC Sample [With Plate Loader]
  128
- Required Materials
  128
- Preparation Process Cytoflex Daily QC Fluorospheres
  128
- Preparation Process Cytoflex IR Daily QC Fluorospheres
  129
- Importing Lot-Specific Target Values
  130
- Collecting QC Data
  134
- Confirming Results
  140
- Creating Levey-Jennings
  143
- QC Result Manager
  145
- Standardization
  146
- Adding, Editing, Importing, and Deleting Standardization Laser
  147
- Adding a New Standardization
  148
- Editing Standardization Item
  149
- Importing a Standardization
  150
- Deleting Standardization
  150
- Applying the Standardization in
  150
CHAPTER 5: Data Acquisition and Sample

155
- Overview
  155
- Creating an Experiment
  155
- Creating an Experiment
  157
- Setting Sample Wells
  161
- Plate Window [Cytoflex LX Shown]
  161
- Modifying Well
  165
- Applying Existing Well Settings to Additional
  166
- Copying, Cutting, and Pasting
  166
- Moving the Location of a
  167
- Running Samples
  167
- Creating a Heat
  169
- Refreshing a Heat
  175
- Modifying Existing Heat Map
  176
- Deleting an Existing Heat
  177
- Exporting a Heat
  177
- Load Sample and Record Data
  178
- Before Running
  178
- Verifying, Selecting, Editing, and Creating Detector
  178
- Configuration
  178
- Sampling and Collecting
  186
- Configuring Acquisition
  190
- Changing the Tube
  190
- Setting the Channel and Creating Plots and
  190
- All Gates - Example
  200
- Circular Gating Logic - Example
  200
- Creating and Adjusting Auto
  202
- Turning Auto Recalculate
  204
- Adjusting Autogate Movement and Laser Settings
  205
- Movement - Max
  206
- Extent - Maximum
  207
- Adjusting the
  209
- Experiment
  227
- Exporting Plots or the Statistics Table of Multiple Tubes as Picture Files
  227
- Importing Instrument
  228
- Exporting Instrument
  228
- Importing and Exporting Compensation
  229
- Saving the Experiment
  232
- Concluding the
  232
CHAPTER 6: Compensation

233
- Overview
  233
- Before Compensation
  233
- After Compensation
  233
- Positive Population Selected from the Unstained
  239
- Positive and Negative Populations Without an Unstained Sample
  240
- Creating a Compensation
  242
- Preparing the Compensation
  245
- Creating the Compensation Matrix from Previously Acquired
  247
- Adjusting Compensation
  248
- Manually Adjusting
  248
- Importing and Exporting
  248
- Files
  248
- Importing Compensation Settings from the Compensation Library
  249
- Exporting Compensation
  251
- Managing the Compensation
  252
- Adding Channels for
  253
CHAPTER 7: Data Review

255
- Overview
  255
- Copying Experiments and Importing
  255
- Copying a Previously Acquired Experiment
  255
- Importing Previously Acquired Data
  255
- Setting the Plots and Statistics
  257
- Opening the Analysis Screen
  257
- Creating Histogram and Dot Plot Overlays
  259
- Calculating Sample Volume and Concentration
  261
- Adjusting Compensation Settings
  262
- Exporting Results
  263
CHAPTER 8: Daily Shutdown

265
- Overview
  265
- Preparing the Cleaning Solution
  265
- Shutting down the Instrument
  265
- Auto Shutdown [Cytoflex LX Only]
  266
CHAPTER 9: Troubleshooting

267
- Overview
  267
- Precautions/Hazards
  267
- Laser Related Hazards
  267
- Laser Beam Hazards
  268
- Laser Warning Labels
  268
- Troubleshooting
  268
- Laser Warning Label on the Laser Optical Bench
  269
- The Cytometer)
  269
- Laser Warning Label Within the Optical Bench
  270
- Laser Warning Label Within the Optical Bench (Located Inside the Cytometer)
  270
- Laser Warning Labels on the Cytometer
  271
- Laser Warning Labels on the Cytometer Back Cover [Cytoflex LX]
  271
- Hazard Labels and Locations
  272
- Biohazard Label and Location
  272
- Biohazard Label on the 4 L Fluid
  272
- Biohazard Label on the 10 L Fluid
  272
- Electrical Shock Hazard Label and Location
  273
- Caution Labels and Location
  273
- Biohazard Label Located in the Sample Station and on the Back
  273
- Electrical Shock Hazard Label by the Power Switch
  273
- Caution Labels
  273
- Plate Loader Hazard Labels and Location
  274
- Disposal of Electrical Instrumentation
  274
- Plate Loader Hazard
  274
- Rohs Notice
  275
- Rohs Caution Label
  275
- Rohs Environmental Label
  275
- Disposal Precaution
  275
- Troubleshooting Table
  276
- Backup and Restore
  289
- Backup
  289
- Restore
  291
CHAPTER 10: Cleaning Procedures

293
- Overview
  293
- Routine Cleaning
  293
- Daily Clean
  293
- Daily Clean [With Plate Loader]
  297
- Cleaning the Sample Station
  299
- Cleaning the Sample Probe
  300
- Deep Clean Procedure
  300
- Cleaning the 4 L Sheath Fluid
  302
- Cleaning the 4 L Waste Container
  303
- Cleaning
  303
- Surface Cleaning and Disinfection
  305
- Preparing the Instrument for Transport or
  306
- Lifting and Carrying
  310
- Replacement/Adjustment Procedures
  313
- Replacement/Adjustment
  314
- Front Cover Removal
  314
- Reinstallation
  315
- Right-Side Cover Removal
  316
- Reinstallation
  317
- Filling the 4 L Sheath Fluid Container
  318
- Replacing the 10 L Sheath Fluid
  319
- Emptying the 4 L Waste Container
  321
- Emptying the 10 L Waste
  322
- Managing the Maintenance
  326
- Adding the Deep Clean Solution
  329
- Replacing the Sheath Fluid
  330
- Replacing the Sample Probe Assembly
  340
- Tube Sample Station
  347
- Inspecting the Liquid Flow Path for Priming the Flow Cell
  354
- Replacing the Plate Holder
  357
- Plate Holder Stage
  357
- Plate Loader Module Removal and Reinstallation
  358
- Removal
  358
- Removing the Tubings from the Fluidics Module to the Plate Loader
  361
- Installation
  362
- Plate Loader Module Securing
  362
- Plate Loader Module Securing
  363
- Connecting the Tubings from the Fluidics Module to the Plate Loader
  364
- Changing the Event Rate Setting
  367
- Replacement/Adjustment
  368
- Calibrating the Sample Flow
  371
- Calibrating the Sample Flow Rate
  371
- Setting Laser Delay
  375
- Replacing the Optical Filter
  376
- Replacing the Fuse
  379
- Replacing the Sheath Fluid Harness And/Or Waste
  381
- Changing Sample Mixing and Backflush Settings
  383
- Calibrating the Plate Position [With Plate Loader]
  385
APPENDIX A: Instrument Installation

391
APPENDIX B: Cytexpert Electronic Record Management

429
- Overview
  429
- Software Menu
  429
- Experiment Management
  430
- Closed File System
  430
- Experiment Directory Management
  431
- Setting up the Experiment Directory
  431
- Folder Hierarchy Management
  432
- Experiment Related Operations
  433
- Importing an Experiment/Template
  434
- Exporting an Experiment/Template
  436
- Log
  439
- Experiment Operation Log
  439
- System Operation Log
  442
- User Management Operation Log
  443
- Electronic Signature
  444
- Signing an Experiment
  444
- Retracting an Experiment Signature
  445
- User Management
  446
- User Administration
  446
- Logging in and out of the Software
  446
- Role Management
  446
- Account Policies
  446
APPENDIX C: Sample Injection Mode Control Kit

448
- Table of Hazardous Substances Name and Concentration
  454
- Table of Hazardous Substances Name and Concentration
  455

Beckman Coulter CytoFLEX S Instructions For Use Manual (348 pages)

Flow Cytometers

Brand: Beckman Coulter | Category: Laboratory Equipment | Size: 28.06 MB

Table of Contents

23
- Revision History
  3
- Alerts for Danger, Warning, and Caution
  17
- Safety Notices
  17
- Safety Precautions
  18
  - Table of Contents
    23
Illustrations

33
- About this Manual
  39
- How to Use Your Manual
  39
- Introduction
  39
- Overview
  39
- Sample Injection Mode Control
  40
- Conventions Used, Xli
  41
- WDM Beam Splitter
  41
- CHAPTER 1 System Overview
  
  43
- Overview
  43
- Product Description
  43
- CHAPTER 2: Using the Cytexpert Software
  
  87
  - Overview
    87
  - Launching the Software
    87
  - Main Software Screen
    87
  - User Management Operation Log
    87
  - Start Page
    88
  - Acquisition Screen
    89
  - Acquisition Screen Navigation
    90
  - Collection
    91
  - Collection [With Plate Loader]
    92
  - Test Tubes
    93
  - Plot Area
    94
  - Status Bar
    95
  - Analysis Screen
    95
  - Drawing Controls Toolbar (Top of Screen)
    96
  - Compensation Experiment Screen
    97
  - QC Experiment Screen
    98
  - QC Report Screen
    98
  - QC Experiment Screen
    99
  - QC Screen Navigation
    99
  - Software Menu
    100
  - Software Menu Tree
    100
  - Acquisition and Analysis Screen Menu
    101
  - QC Software Menu Tree
    101
  - User Management
    109
  - User Manager (Card View)
    109
  - User Manager (Grid View)
    110
  - Creating, Deleting, and Modifying Users in User Manager
    111
  - Unlocking a User Account
    113
  - Resetting a User Passwords
    113
  - Changing a User Password
    113
  - Role Management
    113
  - Role Manager
    114
  - Creating, Deleting, and Modifying User Roles in Role Manager
    115
  - Account Policies
    117
  - Account Policies - Password Policy
    117
  - Account Policies - Account Lockout Policy
    118
  - Account Policies - Application Inactivity Policies
    118
  - User Management Operation Log
    119
  - Graphic and Gating Styles
    120
  - Plots
    120
  - Gates
    121
  - Plate Type Library
    122
  - Adding a Plate Type
    123
  - Editing a Plate Type
    127
  - Duplicating a Plate Type
    128
  - Deleting a Plate Type
    130
  - Software Settings
    131
  - Language Settings
    134
  - Setting up Cytexpert Application Programming Interface (API) Test Client
    134
- CHAPTER 3 Operation Principles
  
  135
  - Overview
    135
  - Sample Flow
    135
  - Sample Loading
    135
  - Hydrodynamic Focusing
    136
  - Laser Beam Shaping
    137
  - Flow Cell
    137
  - Cell Illumination
    138
  - Forward Scatter
    138
  - Side Scatter and Fluorescent Light
    138
  - Light Collection, Separation and Measurement
    138
  - Forward Scatter Collection
    138
  - Laser Beam Shaping
    138
  - Side Scatter and Fluorescent Light Collection
    139
  - Side Scatter
    139
  - Fluorescent Light
    139
  - Light Path through the WDM with a Single Port
    139
  - Signal Processing
    140
  - Data Storage
    140
  - Automated Software Features
    140
  - Parameters
    141
  - TIME Parameter
    141
  - Plot Display
    141
  - Time Vs Fluorescence Plot
    141
  - Statistics
    142
- CHAPTER 4 Daily Startup
  
  143
- CHAPTER 5: Instrument Quality Control and Standardization
  
  171
  - Overview
    171
  - Quality Control
    172
  - Preparing the QC Sample
    173
  - Required Materials
    173
  - Cytoflex Daily QC Fluorospheres Preparation Process
    173
  - Cytoflex Ready to Use Daily QC Fluorospheres Preparation Process
    174
  - Cytoflex Daily IR QC Fluorospheres Preparation Process
    174
  - Preparing the QC Sample [With Plate Loader]
    175
  - Required Materials
    175
  - Preparation Process Cytoflex Daily QC Fluorospheres
    175
  - Cytoflex Ready to Use Daily QC Fluorospheres Preparation Process
    176
  - Cytoflex Daily IR QC Fluorospheres Preparation Process
    176
  - Importing Lot-Specific Target Values
    177
  - Collecting QC Data
    182
  - Collecting QC Data [With Plate Loader]
    185
  - Confirming Results
    190
  - QC Result Manager
    197
  - Standardization
    198
  - Preparing the Standardization Sample
    198
  - Required Materials
    199
  - Preparation Process
    199
  - Generating Target Median Values
    199
  - Adding a New Standardization Item
    206
  - Performing the Standardization
    210
  - Applying the Standardized Acquisition Settings
    213
  - Standardization Target Library
    216
- CHAPTER 6: Data Acquisition and Sample Analysis
  
  219
  - Overview
    219
  - Creating an Experiment
    219
  - Creating an Experiment [Without Plate Loader]
    219
  - Creating an Experiment [With Plate Loader]
    221
  - CSV Template
    224
  - Setting Sample Wells
    227
  - Plate Window [Cytoflex LX Shown]
    227
  - Modifying Well Settings
    232
  - Setting the Channel and Label
    234
  - Creating Plots and Gates
    237
  - All Gates - Example Experiment
    244
  - Circular Gating Logic - Example Experiment
    244
  - Creating and Adjusting Auto Gates
    246
  - Turning Auto Recalculate On/Off
    248
  - Adjusting Autogate Movement and Extent
    249
  - Movement - Default Setting
    249
  - Movement - Max Setting
    250
  - Extent - Default Setting
    250
  - Setting Customized Parameters
    251
  - Extent - Maximum Setting
    251
  - Setting Custom Statistics
    252
  - Configuring Acquisition Settings
    256
  - Changing the Tube Name
    256
  - Laser Settings
    256
  - Laser Setting Window [Cytoflex LX Shown]
    257
  - Target Power Ranges in the Laser Setting Screen
    257
  - Setting Laser Target Power Settings [Cytoflex LX Only]
    258
  - Adjusting the Gain
    259
  - Adjusting the Threshold
    261
  - Setting Collection Conditions
    262
  - Setting Plot Display Conditions
    264
  - Load Sample and Record Data
    265
  - Before Running Samples
    265
  - Verifying, Selecting, Editing, and Creating Detector Configuration
    266
  - Setting up Violet Side Scatter (VSSC) Channel
    270
  - Sampling and Collecting Data [Without Plate Loader]
    273
  - Sampling and Collecting Data [With Plate Loader]
    276
  - Creating a Heat Map [With Plate Loader]
    278
  - Refreshing a Heat Map
    285
  - Modifying Existing Heat Map Settings
    286
  - Deleting an Existing Heat Map
    287
  - Exporting a Heat Map
    287
  - Analyzing and Exporting Data
    288
  - Exporting FCS Files
    293
  - Exporting Plots or the Statistics Table of Multiple Tubes as Picture Files
    295
  - Importing and Exporting Instrument Settings
    296
  - Importing and Exporting Compensation Settings
    298
  - Printing Graphics
    298
  - Saving the Experiment
    301
  - Concluding the Experiment
    301
- CHAPTER 7 Compensation
  
  303
- CHAPTER 8: Data Review
  
  325
  - Overview
    325
  - Copying Experiments and Importing Data
    325
  - Copying a Previously Acquired Experiment
    325
  - Importing Previously Acquired Data
    325
  - Setting the Plots and Statistics
    327
  - Creating Histogram and Dot Plot Overlays
    329
  - Calculating Sample Volume and Concentration
    331
  - Adjusting Compensation Settings
    332
  - Exporting Results
    333
- CHAPTER 9: Daily Shutdown
  
  335
  - Overview
    335
  - Preparing the Cleaning Solution
    335
  - Shutting down the Instrument
    335
  - Auto Shutdown [Cytoflex LX Only]
    336
- CHAPTER 10: Troubleshooting
  
  337

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Beckman Coulter CytoFLEX S Manuals

Beckman Coulter CytoFLEX S Instructions For Use Manual (480 pages)

Table of Contents

CHAPTER 2: Using the Cytexpert Software

CHAPTER 5: Data Acquisition and Sample

CHAPTER 6: Compensation

CHAPTER 7: Data Review

CHAPTER 8: Daily Shutdown

CHAPTER 9: Troubleshooting

CHAPTER 10: Cleaning Procedures

APPENDIX A: Instrument Installation

APPENDIX B: Cytexpert Electronic Record Management

APPENDIX C: Sample Injection Mode Control Kit

Beckman Coulter CytoFLEX S Instructions For Use Manual (348 pages)

Table of Contents

Table of Contents

Illustrations

CHAPTER 1 System Overview

CHAPTER 2: Using the Cytexpert Software

CHAPTER 3 Operation Principles

CHAPTER 4 Daily Startup

CHAPTER 5: Instrument Quality Control and Standardization

CHAPTER 6: Data Acquisition and Sample Analysis

CHAPTER 7 Compensation

CHAPTER 8: Data Review

CHAPTER 9: Daily Shutdown

CHAPTER 10: Troubleshooting

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