Beckman Coulter Allegra 64R Instructions For Use Manual
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Beckman Coulter Allegra 64R Instructions For Use Manual

Compact centrifuge
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GS30-IM-11AA
September 2011
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821
Instructions For Use
Allegra 64R
Compact Centrifuge
®

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  • Page 1 Instructions For Use Allegra 64R Compact Centrifuge ® GS30-IM-11AA September 2011 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821...
  • Page 2 Beckman Coulter, Inc. Find us on the World Wide Web at: www.beckmancoulter.com Beckman Coulter do Brasil Com e Imp de Prod de Lab Ltda Estr dos Romeiros, 220 - Galpao G3 - Km 38.5 06501-001 - Sao Paulo - SP - Brasil CNPJ: 42.160.812/0001-44...
  • Page 3 Safety Notice Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.
  • Page 4 Safety Notice Electrical Safety Electrical Safety To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect this equipment to earth-ground. To preserve this safety feature: • Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the instrument.
  • Page 5 Safety Notice Chemical and Biological Safety Chemical and Biological Safety Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Such materials should not be used in this instrument, however, unless all necessary safety precautions are taken.
  • Page 6 Safety Notice Chemical and Biological Safety GS30-IM-11AA...

  • Page 7: Table Of Contents

    Contents Safety Notice, iii Alerts for Danger, Warning, Caution, and Note, iii Safety During Installation and/or Maintenance, iii Electrical Safety, iv Safety Against Risk of Fire, iv Mechanical Safety, iv Chemical and Biological Safety, v Introduction, xiii Certification, xiii Scope of this Manual, xiii Typographic Conventions, xiv...
  • Page 8 Contents Temperature Sensing and Control, 1-4 Controls and Indicators, 1-4 Power Switch, 1-4 Control Panel, 1-5 Installation Requirements, 2-1 CHAPTER 2: Introduction, 2-1 Preinstallation Requirements, 2-1 Space Requirements, 2-2 Electrical Requirements, 2-3 Test Run, 2-4 Operation, 3-1 CHAPTER 3: Introduction, 3-1 Procedure, 3-1 Preparation and Loading, 3-2...
  • Page 9 Contents Returning a Centrifuge, 5-4 Supply List, 5-4 Replacement Parts, 5-4 Supplies, 5-5 APPENDIX A: Program Library, A-1 Procedure, A-1 Beckman Coulter, Inc. Allegra 64R, GS-15 Series, Allegra 21 Series, and Spinchron 15 Series Centrifuge Warranty...
  • Page 10 Illustrations The Allegra 64R Centrifuge, 1-2 Interior View of the Rotor Chamber, 1-3 The Power Switch, 1-4 The Control Panel, 1-5 Centrifuge Dimensions, 2-2 North American Electrical Socket, 2-3 Installing a Rotor, 3-3 Default Parameters, 3-4 Emergency Door Release, 4-4...
  • Page 11 Tables Tables Acceleration and Deceleration Rates (in seconds per 1000 rpm), 3-7 Error Message Chart, 4-2 Troubleshooting Chart, 4-3...
  • Page 12 Tables...

  • Page 13: Safety Notice

    Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com. Scope of this Manual This manual is designed to familiarize you with the Allegra 64R centrifuge, its functions, specifications, operation, and routine operator care and maintenance. We recommend that you read this entire manual, especially the...

  • Page 14: Typographic Conventions

    CFC-Free Centrifugation To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Allegra 64R centrifuges. Radio Interference This instrument has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of FCC Rules.

  • Page 15: Recycling Label

    For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.

  • Page 16: Specifications

    Introduction Specifications Specifications Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Specification Description Set speed 0 to 30,000 RPM (in 100-RPM increments) or equivalent RCF digital display indicates actual rotor speed 50 RPM (actual RCF can be Speed display selected during operation) Set time...
  • Page 17 Introduction Specifications Specification Description Dimensions • Width — 61 cm (24 in.) • Depth — 66 cm (26 in.) • Height, door closed — 38 cm (15 in.) • Height, door open — 84 cm (33 in.) Weight 102.1 kg (225 lb) Clearances (sides and 7.6 cm (3.0 in.) rear)

  • Page 18: Available Rotors

    Introduction Available Rotors Available Rotors Rotor Part RCF* Number/ Max RPM ( g) Capacity Rotor Manual Rotor Profile Description (g) at 4°C at 4°C (mL) Number 12  2.0/1.5 F1202 30,000 64,400 28,500 58,120 364630 Fixed Angle GS30-TB-006 45° Angle 24 ...
  • Page 19 Introduction Available Rotors Rotor Part RCF* Number/ Max RPM ( g) Capacity Rotor Manual Rotor Profile Description (g) at 4°C at 4°C (mL) Number 4  10 S0410 10,000 10,730 10,000 10 730 364660 Swinging GS30-TB-005 Bucket Four buckets 60  1.5/1.8 H6002 12,200 12,400...
  • Page 20 Introduction Available Rotors GS30-IM-11AA...

  • Page 21: Description

    CHAPTER 1 Description Introduction This section provides a brief physical and functional description of the Allegra 64R centrifuge. The operating controls and indicators are also described; instructions for their use are in CHAPTER 3. Chemical compatibilities of materials listed in this manual can be found in Chemical Resistances (publication IN-175).

  • Page 22: Safety Features

    Name Rating Plate Figure 1.1 The Allegra 64R Centrifuge Safety Features Allegra 64R centrifuges have been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft). Instrument safety features include: • The door has dual electromechanical door-locking mechanisms to prevent operator contact with spinning rotors.

  • Page 23: Chassis

    Description Chassis Chassis Housing The centrifuge housing is made of sheet steel, finished with urethane paint. The control panel is covered by a protective overlay made of coated polycarbonate. Door The solid-sheet aluminum door is secured to the housing by solid shafts. A dual electromechanical door lock system prevents operator contact with spinning rotors and prevents run initiation unless the door is shut and latched.

  • Page 24: Drive

    Description Controls and Indicators Drive The asynchronous three-phase direct-drive motor is brushless for clean, quiet operation. A tie- down screw is used to attach the rotor to the drive shaft. The resilient suspension ensures that loads will not be disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs during centrifugation.

  • Page 25: Control Panel

    Description Controls and Indicators Control Panel The control panel (Figure 1.4), mounted at an angle on the centrifuge front for easy visibility and access, comprises touch keys—system keys and parameter keys—and digital displays. The panel also contains an light that flashes if rotor loads are severely out of balance. IMBALANCE Figure 1.4 The Control Panel SPEED...
  • Page 26 Description Controls and Indicators OPEN DOOR Pressing the OPEN DOOR key unlatches the centrifuge door locks and allows the door to be opened. The centrifuge will accept this command only when the rotor is completely stopped and the OPEN DOOR key LED is lit. PULSE Pressing the PULSE key causes the installed rotor to accelerate up to the set speed for short-duration runs (as long as the key is pressed).
  • Page 27 Description Controls and Indicators TIME The TIME key is used to select the run duration. When the TIME key is pressed, the last digit on the TIME display flashes, indicating that the time can be entered with the cursor keys. •...
  • Page 28 Description Controls and Indicators SPEED • In input mode the SPEED display shows the value of the parameter being set, depending on the programming key pressed (RPM, RCF, or ROTOR). For example, if the ROTOR programming key is pressed, a rotor number appears on the SPEED display.

  • Page 29: Chapter 2: Installation Requirements

    CHAPTER 2 Installation Requirements Introduction Do not attempt to install this centrifuge. Its purchase price includes installation by Beckman Coulter personnel. Installation by anyone other than an authorized Beckman Coulter representative invalidates any warranty covering the instrument. WARNING This centrifuge weighs 102.1 kg/225 lb. DO NOT attempt to lift or move it without assistance from another person.

  • Page 30: Space Requirements

    Installation Requirements Space Requirements Space Requirements Select a location away from heat-producing laboratory equipment, with sufficient ventilation to allow for heat dissipation. Position the centrifuge on a level surface, such as a sturdy table or laboratory bench that can support the weight of the centrifuge (102.1 kg /225 lb) and resist vibration. In addition to space for the centrifuge itself, allow 7.6-cm (3-in.) clearances at the sides and back of the centrifuge to ensure sufficient air circulation.

  • Page 31: Electrical Requirements

    Installation Requirements Electrical Requirements Use the anti-rotation kit to secure the centrifuge to the bench or table as described in publication GS30-TB-003. The anti-rotation system is designed to reduce the possibility of injury or damage that could result from instrument movement in the event of a major rotor mishap. NOTE During transport between areas with varying temperatures, condensation may occur inside the centrifuge.

  • Page 32: Test Run

    Installation Requirements Test Run Test Run NOTE The centrifuge must be plugged in and the power switch turned to on position ( ) before the door can be opened. We recommend that you make a test run to ensure that the centrifuge is in proper operating condition following shipment.

  • Page 33: Chapter 3: Operation

    Operation Introduction This section contains operating procedures for the centrifuge, using any of the Beckman Coulter rotors designed for use in this centrifuge. Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation. To prevent condensation, keep the centrifuge door closed and the power turned off ) when the centrifuge is not in use.

  • Page 34: Preparation And Loading

    Operation Run Procedure Preparation and Loading For fast temperature equilibration, cool or warm the rotor to the required temperature before the run. NOTE For high-speed runs at temperatures of 20°C or higher, prime the refrigeration system by running the instrument at 10°C for 5 to 10 minutes beforehand to prevent overheating. Check the name rating plate for the correct voltage, then plug the power cord into the wall receptacle.

  • Page 35: Installing A Rotor

    Operation Run Procedure Figure 3.1 Installing a Rotor 1. Swinging Bucket Rotor 4. tighten the tie-down screw to the right (clockwise) onto the drive shaft 2. Fixed Angle Rotor 5. attach the lid and tighten with the T-handle wrench 3. Lower the rotor straight down onto the drive shaft 6.

  • Page 36: Entering Run Parameters

    Operation Run Procedure Close the centrifuge door and push firmly down on both sides of the door front until you hear both latches engage. Entering Run Parameters When the power is applied for the initial use (no previous runs), default values will be displayed (see Figure 3.2).
  • Page 37 Operation Run Procedure Press the key. ROTOR • The number of the last rotor previously centrifuged appears on the display. SPEED Press the  or  cursor key until the number of the rotor in use is displayed. Press ENTER •...
  • Page 38 Operation Run Procedure Setting RCF Press the key. • The last digit on the display ( ) flashes, indicating that the RCF can be entered. SPEED Press the  or  cursor key until the required RCF is displayed. • The corresponding RPM will be automatically calculated and the centrifuge will run at the calculated speed.
  • Page 39 Operation Run Procedure Setting Run Temperature Run temperature can be set between –20 and +40°C. NOTE Temperatures may vary slightly between instruments. If sample temperature is crucial, test temperature settings on your instrument using water samples. Press the key. TEMP •...

  • Page 40: Starting A Run

    Operation Run Procedure Press the key. ACCE • The display flashes, indicating that the selected rate number can be entered with ACC/DEC the cursor keys. Press the  or  cursor key until the required number is displayed. Setting Deceleration Rate key is used to select deceleration rates that will maintain optimum separation.

  • Page 41: Changing Parameters During A Run

    Operation Run Procedure a. The error must be cleared and an appropriate speed entered before the centrifuge can be started. • Throughout the run, checks are made to ensure that the rotor does not exceed set speed. • The display indicates the rotor speed in RPM. SPEED (The RCF can be checked by pressing the key.)

  • Page 42: Stopping A Run

    Operation Unloading Press the key. ENTER • The current RPM value will be displayed, changing to the new value as the rotor accelerates or decelerates to the new speed selected. • The corresponding RCF will be automatically calculated by the centrifuge. Stopping a Run A timed run will end automatically when the display counts down to zero.

  • Page 43: Summary Of Run Procedures

    Operation Summary of Run Procedures Summary of Run Procedures For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration. For low-temperature runs, precool the centrifuge by running a 30-minute cycle (with a precooled rotor installed) at the required temperature with the speed set at 2000 RPM.
  • Page 44 Operation Summary of Run Procedures Unload the rotor according to instructions in the appropriate rotor manual. CAUTION If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. Apply appropriate decontamination procedures to the centrifuge and accessories. 3-12 GS30-IM-11AA...

  • Page 45: Chapter 4: Troubleshooting

    Table 4.1 to determine the nature of the problem and recommended actions. If you are unable to correct the problem, call Beckman Coulter Field Service. To help diagnose and correct the problem, provide as much information as you can: • The error number that appears on the display.
  • Page 46 Beckman Coulter Field Service. Rotor recognized but Maximum Input correct parameters for the rotor in use, incorrect deceleration to full then restart. stop a. If the recommended action does not correct the problem, call Beckman Coulter Field Service. GS30-IM-11AA...

  • Page 47: Other Possible Problems

    Possible causes for each problem are listed in the probable order of occurrence. Perform the recommended corrective action in sequence, as listed. If you are unable to correct the problem, call Beckman Coulter Field Service. Table 4.2 Troubleshooting Chart...

  • Page 48: Emergency Access

    Troubleshooting Emergency Access Emergency Access If the facility power fails only momentarily, the centrifuge will resume operation when power is restored and the rotor will return to set speed. However, if the rotor comes to a complete stop you will have to restart the run when the power is restored. In the event of an extended power failure, it may be necessary to trip the door-locking mechanism manually to remove the rotor and retrieve your sample (see Figure...
  • Page 49 Troubleshooting Emergency Access Make sure that the rotor is not spinning. Do not proceed if there is any sound or vibration coming from the drive. Use the 2.5-mm ball-head Allen wrench (964774) to loosen the four screws on the centrifuge front panel (see Figure 4.1).
  • Page 50 Troubleshooting Emergency Access GS30-IM-11AA...

  • Page 51: Chapter 5: Care And Maintenance

    CHAPTER 5 Care and Maintenance Introduction For maintenance not covered in this manual, call Beckman Coulter Field Service. User messages are discussed in CHAPTER 4, Troubleshooting. Refer to the applicable rotor manual and to Chemical Resistances (publication IN-175) for instructions on the care of rotors and their accessories.

  • Page 52: Cleaning

    Care and Maintenance Cleaning Use a sponge to wipe condensation out of the chamber bowl between runs to prevent chamber icing. If chamber icing occurs, defrost before use. To prevent the rotor from sticking, lubricate the drive shaft with Spinkote at least once a month, and after each cleaning.

  • Page 53: Tube Breakage

    Chemical Resistances for more information regarding chemical resistance of centrifuge and accessory materials. While Beckman Coulter has tested these methods and found that they do not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 54: Storage And Transport

    Authorization form) must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the RGA form and for packaging and shipping instructions. To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from pathogens and/or radioactivity.

  • Page 55: Supplies

    Care and Maintenance Supply List Supplies NOTE For MSDS information, go to the Becman Coulter website at www.beckmancoulter.com. Spinkote lubricant (2 oz) 306812 Silicone vacuum grease (1 oz) 335148 Beckman Solution 555 (1 qt) 339555 GS30-IM-11AA...
  • Page 56 Care and Maintenance Supply List GS30-IM-11AA...

  • Page 57: Appendix A: Program Library

    APPENDIX A Program Library Procedure Record the program parameters on the chart below as a quick reference when duplicating run conditions. Refer to CHAPTER 3 of this manual for the numbers representing acceleration and deceleration (ACCEL/DECEL) rates. Speed Time Program (hrs/ DECEL Temp...
  • Page 58 Program Library Procedure Speed Time Program (hrs/ DECEL Temp ACCEL Number User Date Purpose min) Rate (°C) Rate GS30-IM-11AA...

  • Page 59: Beckman Coulter, Inc

    (1) year after delivery of an Allegra 64R, Allegra 21, GS-15, or Spinchron 15 series centrifuge (the product), to the original buyer by Beckman Coulter or by an authorized representative, provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use.
  • Page 60 Beckman Coulter, Inc. Allegra 64R, GS-15 Series, Allegra 21 Series, and Spinchron 15 Series Centrifuge Warranty Warranty-2 PN 7654321A...
  • Page 63 Symbol Simbolo Symbol Title Titel Titre Titulo Titolo Symbole Símbolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung)
  • Page 64 © 2011 Beckman Coulter, Inc. All Rights Reserved...

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