Beckman Coulter CytoFLEX Series Instructions For Use Manual page 246

Flow cytometers
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Compensation
Creating the Compensation Matrix from Previously Acquired Data
4
Save To Compensation Library
Select
compensation library.
5
Specify the key words and select
NOTE
The settings stored in the compensation library are specific to the detector configuration. The
compensation library can only be applied when the detector configurations are the same.
At any time, saved compensation experiments can be reopened and the compensation values
recalculated.
6
Close
Select
Creating the Compensation Matrix from Previously Acquired Data
The software supports importing single color data acquired from other experiments into a
compensation experiment to perform compensation calculations. The data to be imported must
match the active detector configuration at the time that the compensation experiment was created.
Otherwise, the data cannot be imported. It is important to ensure that imported data comes from
the same instrument and uses the same configuration and channels. Data originating from a
different instrument will cause erroneous calculations.
1
Select
New Compensation
2
To create a compensation experiment, select the required channels. Refer to
Channel and Label
6-14
to save the single color compensation values in the
OK
.
.
from the File menu or the start page.
in
CHAPTER 5, Data Acquisition and Sample
Setting the
Analysis.
B49006AH

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