Related Manuals for Beckman Coulter J-Lite JLA 16.250
Summary of Contents for Beckman Coulter J-Lite JLA 16.250
- Page 1 J-TB-072JB June 2018 J-Lite JLA-16.250 Fixed Angle Rotor Used In Beckman Coulter J2 and Avanti J Series Centrifuges © 2018 Beckman Coulter, Inc.
- Page 2 Beckman Coulter, the stylized logo, and the Beckman Coulter prod- uct and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. All other trademarks, service marks, products, or services are trade-...
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Page 3: Safety Notice
This rotor was developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a non-Beckman Coulter centrifuge or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter’s approval. -
Page 4: Specifications
J-Lite JLA-16.250 FIXED ANGLE ROTOR SPECIFICATIONS Maximum speed (Avanti J series centrifuges) ....16 000 rpm Maximum speed (J2 series centrifuges) ....14 000 rpm Critical speed range* . - Page 5 DESCRIPTION This rotor has been manufactured in a registered ISO 9001 or 13485 facility for use in Beckman Coulter Avanti J and J2 series centrifuges. The J-Lite JLA-16.250 fixed angle rotor (see Figure 1), rated for 16 000 rpm in Beckman Coulter Avanti J series centrifuges and...
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Page 6: Preparation And Use
JLA-16.250 rotor with dual-locking lid was tested to demonstrate containment of microbiological aerosols under normal operating conditions of the associated Beckman Coulter centrifuge, when used and maintained as instructed. The rotor is also available with a single- locking lid. The JLA-16.250 rotor has a patented fluid-containment annulus, located below the O-ring sealing surface. -
Page 7: Prerun Safety Checks
2. Inspect the rotor drive pins to ensure that they are not damaged (see instructions on page 20). If the drive pins appear to be damaged, contact Beckman Coulter Field Service. 3. Verify that the bottles or tubes being used are listed in Table 3. - Page 8 CENTRIFUGE BEFORE USING THE JLA-l6.250 ROTOR IN A MICROPROCESSOR-CONTROLLED J2 SERIES CENTRIFUGE If you have a microprocessor-controlled Beckman Coulter J2 series centrifuge (model J2-MI, J2-21M, J2-21M/E, or J2-MC), you will need to enter the JLA-16.250 rotor identification code (14) and maximum speed (l4 000 rpm) into the instrument memory before the first use of the JLA-16.250 rotor in that instrument.
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Page 9: Installing The Rotor
J-Lite JLA-16.250 Rotor 2. The rotor display should now show 2 – – . – . You are now ready to program the second entry, the JLA-16.250 rotor’s maximum speed in J2 series centrifuges, which is l4 000 rpm. Enter 14, again press and hold [ ], press [ ], and release both keys. - Page 10 J-Lite JLA-16.250 Rotor 5. Once the rotor is properly aligned carefully lower it straight down Tie-down Knob onto the hub. Do not drop the rotor onto the hub. 6. Slowly turn the rotor around the drive spindle to make sure that it is properly seated.
- Page 11 J-Lite JLA-16.250 Rotor TEMPERATURE To ensure that the JLA-16.250 rotor reaches the required temperature during the run, follow the appropriate instructions below for the J centrifuge model being used. Avanti J Series Centrifuges (Except Avanti J-E) Enter the run temperature according to the instructions in your centri- fuge instruction manual.
- Page 12 J-Lite JLA-16.250 Rotor J2 Series Centrifuges Enter the required run temperature and the appropriate temperature compensation units (see Tables 1 and 2) on the centrifuge control panel following the instructions below. Table 2. Temperature Compensation Settings for Analog and Microprocessor-Controlled Centrifuges (Models J2-HS, J2-21, J2-MI, J2-21M, J2-MC, and J2-21M/E).
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Page 13: Run Procedure
J-Lite JLA-16.250 Rotor Microprocessor-Controlled J2 Series Centrifuges (Models J2-MI, J2-21M, J2-MC, and J2-21M/E) Enter rotor code 14, then follow the steps below. 1. Press the [ ] key on the centrifuge control panel and then use TEMP the keypad to enter the sample temperature. 2. -
Page 14: Removal And Sample Recovery
J-Lite JLA-16.250 Rotor CAUTION Make sure that the rotor is properly seated on and securely fastened to the drive hub before each run. When the rotor is properly loaded and installed in the centrifuge, you are ready to perform the run. Refer to the applicable centrifuge instruction manual for operating instructions. - Page 15 J-Lite JLA-16.250 Rotor 4. Grasp the daisy knob and turn it to the left (counterclockwise) until it disengages. 5. Remove the lid and set it aside. If the lid cannot be removed, first pull up on the tie-down knob to release vacuum inside the rotor, and then remove the lid.
- Page 16 J-Lite JLA-16.250 Rotor container.) To minimize the possibility of leakage from capped tubes or bottles, load tubes or bottles with sample, secure the caps, and precool the loaded, sealed labware to run temperature before beginning the run. Refer to Appendix A in Rotors and Tubes for information on the chem- ical resistances of tube and accessory materials.
- Page 17 10 700 Maximum speeds listed are guidelines only. These speeds have been achieved in reliability tests at Beckman Coulter, but because of manufacturing variances no guarantee of performance or fit is expressed or implied. (The maximum rotor speed in J2 series centrifuges is 14 000 rpm.)
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Page 18: Run Times
J-Lite JLA-16.250 Rotor Thickwall Tubes Thickwall polypropylene and polycarbonate tubes can be run partially filled (at least half filled) with or without caps, but all opposing tubes for a run must be filled to the same level with liquid of the same den- sity. - Page 19 J-Lite JLA-16.250 Rotor For centrifugation below maximum speed, estimate the run time required by adjusting the k factor as follows: maximum speed ---------------------------------------- - actual run speed Run times can also be estimated from data established in prior experiments using a different rotor if the k factor of the previous rotor is known.
- Page 20 J-Lite JLA-16.250 Rotor Table 5. Relative Centrifugal Fields for the JLA-16.250 Rotor. Entries in this table are calculated from the formula RCF = 1.12r (RPM/1000) and are then rounded to three significant digits. Relative Centrifugal Field (× g) Rotor at r at r at r Speed...
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Page 21: Care And Maintenance
If any of these signs are evident, do not use the rotor. Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program (FRIP) and the rotor repair center. -
Page 22: Maintenance
O-rings caused by a tightened lid may reduce O-ring life. Refer to Appendix A in Rotors and Tubes for the chemical resistances of rotor and tube materials. Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center. - Page 23 If the rotor (and/or accessories) becomes contaminated with radioac- tive material, decontaminate it using a solution that will not damage the anodized surfaces. Beckman Coulter has tested a number of solutions and found several that do not harm anodized aluminum: RadCon Surface Spray or IsoClean Solution (for soaking), Radiacwash.
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Page 24: Sterilization And Disinfection
J-Lite JLA-16.250 Rotor While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfec- tion is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 25: Returning A Rotor
Before returning a rotor or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. This RGA form, which may be obtained from your local Beckman Coulter sales office, should contain the following information: •... -
Page 26: Supply List
SUPPLY LIST Call Beckman Coulter Sales (1-800-742-2345 in the United States) or your local Beckman Coulter office, or see the Beckman Coulter High Performance, High Speed, High Capacity Rotors, Tubes & Accesso- ries catalog (BR-8102, available at www.beckman.com) for detailed information on ordering parts and supplies. - Page 27 J-Lite JLA-16.250 Rotor...
- Page 28 J-Lite JLA-16.250 Rotor...
- Page 29 CONDITIONS clause of the Beckman Coulter, Inc., terms and conditions of sale in effect at the time of sale, Beckman Coulter, Inc. agrees 1. Except as otherwise specifically provided herein, this war- to correct either by repair, or, at its election, by replacement, ranty covers the rotor only and Beckman Coulter, Inc.
- Page 30 Beckman Coulter, Inc. • 250 S. Kraemer Blvd. • Brea, California 92821 U.S.A. Sales and Service: 1-800-742-2345 • Internet: www.beckman.com • Printed in U.S.A. © 2018 Beckman Coulter, Inc. All rights reserved...