Beckman Coulter CytoFLEX Series Instructions For Use Manual page 236

Compensation
Creating a Compensation Experiment
4
OK
Select
After confirmation, the software automatically generates the following compensation
experiment.
NOTE
Select Area to calculate compensation based on the Area measured. Alternatively, select Height
to calculate compensation based on the Height measured.
Preparing the Compensation Sample
To perform a compensation experiment, prepare:
• A single positive control tube for each color
•
A negative control tube (optional)
NOTE
A negative control tube is required if a single positive control tube does not contain a negative
population.
For the negative control sample and single positive control sample, you can use blood, cells, or
dedicated compensation beads such as VersaComp Antibody Capture Beads. For details, refer to the
appropriate reagent instructions for use. The negative control tube is used to determine the
autofluorescence of the sample.
Using Control Samples to Generate the Compensation Matrix
Defining the Negative Population Using Unstained Samples
1
Confirm that the instrument has been initialized. Refer to
CHAPTER 3, Daily
6-4
.
Startup.
Initializing the Instrument in
B49006AH