Beckman Coulter CytoFLEX Series Instructions For Use Manual page 234

Compensation
Creating a Compensation Experiment
Workflow:
Prepare
Compensation
Samples
This chapter contains information on:
•
Creating a Compensation Experiment
•
Creating a Compensation Experiment [With Plate Loader]
•
Creating the Compensation Matrix from Previously Acquired Data
•
Adjusting Compensation
Creating a Compensation Experiment
Before creating a compensation experiment, you must verify the instrument's detector
configuration settings (see
CHAPTER 5, Data Acquisition and Sample
1
Select New Compensation
experiment.
NOTE
The file name of the newly created compensation experiment has a ".xitc" suffix.
2
Navigate to the desired file path and select
CAUTION
Risk of erroneous results. Select an unstained tube, according to which the
fluorescence background will be set. If there is not an unstained tube
available, then each single color tube must have a negative population.
It is important to specify the appropriate sample type. Otherwise, the
background information could be incorrectly calculated and lead to erroneous
compensation results.
3
Select the channel requiring compensation calculation and the sample type.
6-2
Create
Ô compensation Ô
experiment
Verifying, Selecting, Editing, and Creating Detector Configuration
Analysis).
in the File menu or on the start page to create a new compensation
Acquire and Calculate
Ô
record data compensation
Save
. The Compensation Setup window appears.
Apply
compensation
Ô
to sample for
data analysis
in
B49006AH