Sign In
Upload
Manuals
Brands
Beckman Coulter Manuals
Laboratory Equipment
JCF-Z
User Manuals: Beckman Coulter JCF-Z Flow Rotor
Manuals and User Guides for Beckman Coulter JCF-Z Flow Rotor. We have
1
Beckman Coulter JCF-Z Flow Rotor manual available for free PDF download: Instructions For Use Manual
Beckman Coulter JCF-Z Instructions For Use Manual (104 pages)
Zonal and Continuous Flow Rotor
Brand:
Beckman Coulter
| Category:
Laboratory Equipment
| Size: 6 MB
Table of Contents
Revision History
3
Alerts for Danger, Warning, Caution, Important, and Note
5
Safety Notice
5
Table of Contents
9
CHAPTER 1 The Process of Continuous Flow and Zonal Centrifugation
16
Cross Section of Zonal Rotor with Standard Zonal Core
17
Zonal Centrifugation
17
JCF-Z Zonal Applications
18
Cross Section of a Continuous Flow Rotor
16
JCF-Z Continuous Flow Applications
16
Continuous Flow Centrifugation
16
CHAPTER 2 Description
19
Rotor Components
19
Rotor Components
21
Seal and Transfer Assemblies
21
Identifying O-Rings. O-Rings Requiring Occasional Replacement Are Shown, Actual Size
23
Specifications
24
Different Core Specifications
25
CHAPTER 3 Assembly and Installation
27
Installing the Zonal Bracket (Avanti J Series Centrifuges Only)
27
Installing the Zonal Interlock Accessory
27
Tools and Supplies
27
Tools Provided
27
Supplies Required but Not Provided
28
Tools Provided in Tool Kit 335143
28
Assembling the Rotor
29
Step 1
29
Step 1A
29
Step 1B
30
Step 1C
30
Step 1D
31
Step 2
32
Step 4
34
Step 5
35
Step 6
36
Step 6A
36
Step 6B
38
Step 7
39
Standard Zonal and Continuous Flow Rotors
39
Standard Seal Housing
39
High Flow Seal Housing
40
Step 8
40
Standard Zonal and Continuous Flow Rotors
40
Step 9
41
Step 9A
41
Step 9B
42
Step 10
43
Step 11
44
Step 13
45
Assembly
45
Tubing
45
CHAPTER 4: Preparing for Operation
47
Determining the Rotor Speed
47
Determining the Flow Rate: Large and Standard Pellet Cores
48
Setting the Temperature
51
Avanti Series Centrifuges
51
Microprocessor-Controlled J2 and J6 Series Centrifuges (Models J2- M/E)
51
Analog J2 and J6 Series Centrifuges (Models J2-HS, J2-21, J2-HC, J6-HC, and J-6B)
52
Determining the Liquid Pressure
52
Determining Appropriate Gradients
52
J2 and J6 Series Centrifuge Temperature Settings. All Settings
52
Radius Versus Volume for Standard Pellet Core, Standard Zonal
53
CHAPTER 5: Operation
55
Operating Precautions
55
Continuous Flow Operation
56
Pelleting Technique
56
Pelleting Technique, Continuous Flow Cores
56
Run Procedure for Pelleting Sample (Continuous Flow Cores)
57
Rotor Installation
57
Line Connections, Continuous Flow Cores. Using this Tubing
58
Arrangement for Basic Pelleting against the Rotor Wall
58
Purging Air from the System (Continuous Flow Rotor Only)
59
Density Gradient Operation (Continuous Flow Cores)
60
Gradient or Cushion Separation Techniques
60
Forming an Air Block
61
Two Techniques for Forming an Air Block During Density
61
Gradient or Cushion Separation Technique, Continuous Flow
62
Technique B, Described in Table 5.1, Is Shown
62
Run Procedure for Separating in a Gradient or Cushion (Continuous Flow Cores)
63
Zonal Operating Techniques and Run Procedures
63
Standard Zonal Core
63
Sucrose Gradient after One Hour, Monitored by Refractometer
63
Line Connections, Standard Zonal Core
64
Operating Procedure, Standard Zonal Core
65
Removing and Reinserting Fittings and Liquid Lines
66
Reorienting Gradient (Reograd) Zonal Core
67
Line Connections, Reograd Zonal Core
67
Ending the Run
68
Continuous Flow Core Containing Pellet
68
Operating Procedure, Reograd Zonal Core
69
Lowering Rotor Onto Plug. Rotor Contents will Spill out
70
As Soon as the Core Is Removed if the Plug and Plug O-Ring
70
Are Not in Place
70
Disassembling the Rotor. Disassemble as Shown to Remove
71
(Arrows Show Direction of Tool Use). See CHAPTER 6, Care and
71
For Instructions on Inspecting, Cleaning, and Replacing the Bearings
71
Continuous Flow Core Containing Gradient or Cushion
72
Removing Small Pellet. Lift out Canoe-Shaped Pellet Container
72
Scrape out Pellet
72
Standard Zonal Core
73
Reograd Zonal Core
74
Flushing the Tubing Lines
75
CHAPTER 6: Care and Maintenance
77
Maintenance
77
Cleaning
77
Decontamination
78
Sterilization and Disinfection
79
Inspecting the Bearings
79
Smoothing (Lapping) the Rotating Seal
80
Returning a Rotor
80
Supply List
81
CHAPTER 7 Troubleshooting
83
Leakage
83
Rotating Seal/Stationary Seal Interface
83
Scavenger Line
83
Other Areas
83
Mixing of Inlet and Outlet Liquid
83
Vibration
84
APPENDIX A: Assembling the Rotating Seal
85
Assembling the Rotating Seal
85
Transfer Tube Assembly
85
Fully Assembled Transfer Tube
87
Transfer Tube Assembly and Rotating Seal
87
Correct Position of the Rotating
88
Incorrect Position of the Rotating Seal (Wrong Spring Tension
89
Bearing Housing
89
Stationary Seal Housing Mated to Bearing Housing
90
APPENDIX B: Using the JCF-Z Continuous Flow/Zonal
93
Description
93
Avanti J Series Centrifuge Chamber with JCF-Z Rotor and Zonal
93
Zonal Bracket Kit Contents
94
Basic Kit 363843 or 366431
94
Kit 363844 Contents
94
Tubing
94
Kit 363845 Contents
94
Assembly Instructions
95
Step 1. Install the Rotor
95
Step 2. Attach the Zonal Bracket to the Centrifuge Chamber Wall
95
Step 3. Insert the Tubing through the Door Gasket
95
Sizes 14 and
96
Tubing
96
Step 4. Attach the Tubing to the Zonal Bracket
97
Step 5. Attach Tubing to the JCF-Z Seal Assembly Housing
98
Attaching the Tubing Clamps to the B.7 Inserting Tubing and Feed Fittings into the JCF-Z Seal
98
Step 6. Attach a Scavenger Line (Optional
99
Attaching a Scavenger
100
JCF-Z Rotor Warranty and Seal Assembly Warranty
101
Using the JCF-Z Continuous Flow/Zonal Rotor Bracket (Avanti J Series Centrifuges Only
93
Advertisement
Advertisement
Related Products
Beckman Coulter Avanti J-26 XP Series
Beckman Coulter Avanti JXN-26
Beckman Coulter J2 series
Beckman Coulter J2-MC
Beckman Coulter J2-HC
Beckman Coulter J6-HC
Beckman Coulter Avanti J-26XPI
Beckman Coulter JS-4.750
Beckman Coulter Avanti J-26S XPI
Beckman Coulter JA-25.50
Beckman Coulter Categories
Laboratory Equipment
Measuring Instruments
Medical Equipment
Cash Counter
Desktop
More Beckman Coulter Manuals
Login
Sign In
OR
Sign in with Facebook
Sign in with Google
Upload manual
Upload from disk
Upload from URL