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USING REGIONS
ASSIGNING REGIONS
Assigning Gates to Histograms
The "gating" process consists of:
Creating regions appropriate for the types of
analyses you are doing.
Assigning the regions as gates so that other
histograms include data from only those
regions.
The software lets you create up to 24
regions in a protocol. You can use:
Up to 16 of those 24 regions to gate
r
other histograms. Each rectilinear
region counts as two regions.
The not (—) sign to exclude those
r
events in a region.
IMPORTANT
incorrect results. Use only amorphous or linear regions for — (not) gating.
To the right is an example of — (not) gating
with a rectilinear region which shows why
you should not use this type of gating.
Note: "OR" gating is not supported.
5-6
If you use rectilinear gates for — (not) gating you can exclude desired populations and report
7021279B
PN 4237238BA
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