Agilent Technologies 1100 Series Reference Manual page 34

2 How to optmize the Detector
Figure 8
Traditionally LC analysis with UV detectors is based on comparing
measurements with internal or external standards. To check photometric
accuracy of the Agilent 1100 DAD/MWD it is necessary to have more precise
information on path lengths of the flow cells.
The correct response is:
expected response * correction factor
Please find below the details of the Agilent 1100 DAD/MWD flow cells:
Table 5 Correction factors for Agilent 1100 DAD/MWD flow cells
Flow cell type
Standard flow cell
Semi-micro flow cell
80 Nanoliter flow cell kit
500 Nanoliter flow cell kit
High Pressure flow cell
34
Time (min)
Influence of Cell Path Length on Signal Height
Cell volume Part number
13 µl G1315-60012
5 µl G1315-60011
0.08 µl G1315-68716
0.5 µl G1315-68724
1.7 µl G1315-60015
Analysis of pesticide standard
6-mm optical path length
10-mm optical path length
Path length Path length
(nominal) (actual)
10 mm 9.80 ± 0.07 mm
6 mm 5.80 ± 0.07 mm
6 mm 6.00 ± 0.02 mm
10 mm 10.00 ± 0.02 mm
6 mm 5.75 ± 0.07 mm
1100 Series DAD and MWD Reference Manual
Correction
factor
10/9.8
6/5.8
6/6
10/10
6/5.75