Agilent Technologies 1100 Series Reference Manual page 32

2 How to optmize the Detector
Table 4 Optimization Overview, continued
Parameter
5 Setting the slit width
• Use 4 nm slit for normal applications.
• Use narrow slit (e.g 1 nm) if your analytes have narrow absorbance
bands and for high concentrations.
• Use a wide slit (e.g. 16 nm) to detect very low concentrations.
• Optimizing spectral acquisition (DAD only)
• Select spectra acquisition mode according to your needs (see
for storing spectra" on page 42).
• Set the spectral wavelength range (for colorless samples 190...400 nm
is sufficient).
• Set step to 4 nm for normal use; set small step (and slit width) if high
resolution of spectra with fine structure is wanted.
Typical column Typical peak
length width
T<= 5 cm 0.025 min
10 cm 0.05 min
20 cm 0.1 min
>= 40 cm 0.2 min
Typical flow
rate
Internal column diameter
Figure 7
32
Recommended flow cell
500 nl
flow cell
Semi-mirco
flow cell
0.2 ... 0.4
0.01 ... 0.2
ml/min
ml/min
0.5 ... 1 mm 2.1 mm
Choosing a Flow Cell
Impact
• spectral resolution, sensitivity and
linearity.
"Options
Standard
flow cell
1 ... 2 ml/min
0.4 ... 0.4
ml/min
3.0 mm 4.6 mm
1100 Series DAD and MWD Reference Manual
High pressure
flow cell for
pressures
above 100 bar
0.01 ... 5
ml/min