Absorbance Accuracy - tecan infinite 200 Instructions For Use Manual

Calculation of the Sensitivity (Detection Limit):
8.3.6

Absorbance Accuracy

2008-07
DetectionL imit ( fmol
-8
2*10 Concentration of ATP standard [M]
Stdev Standard deviation of Blank
B
mean Average of wells filled with ATP standard
ATP
mean Average of Blank wells
B
0.0002 Conversion into mol/well
-15
1/1e Conversion into fmol/well
The result of the formula determines the detection limit in fmol/well.
Use MultiCheck Plate – For details please refer to the MultiCheck Instructions for
Use.
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
⋅ −
8
2 10 * 3 *
=
/ well )
−
mean mean
ATP
8. Quality Control
Stdev
B
* . 0 0002 *
e 1
B
1
−
15
141