Definition Of The Detection Limit; Fluorescein (Fluorescence Intensity) Top; Fluorescein (Fluorescence Intensity) Bottom; Europium (Time Resolved Fluorescence) - tecan infinite 200 Instructions For Use Manual

7. Instrument Features
7.3.1

Definition of the Detection Limit

7.3.2

Fluorescein (Fluorescence Intensity) Top

7.3.3

Fluorescein (Fluorescence Intensity) Bottom

7.3.4

Europium (Time Resolved Fluorescence)

118
The detection limit is the fluorophore concentration where the background-
subtracted signal equals 3 times the standard deviation of the background noise.
When selecting 1 flash per well, the plate carrier does not stop at the
measurement position. Using more flashes per well may improve the detection
limit, but the total measurement time will be longer.
Plate Type (number of wells)
Dispensed Volume [µl]
Flashes (Reads) per Well
Fluorescein Detection Limit [pM]
Uniformity at 25 nM Fluorescein
Reproducibility at 25 nM Fluorescein
Plate Type (number of wells)
Dispensed Volume [µl]
Flashes (Reads) per Well
Fluorescein Detection Limit [pM]
Uniformity at 25 nM Fluorescein
Reproducibility at 25 nM Fluorescein
Plate Type (number of wells)
Dispensed Volume [µl]
Flashes (Reads) per Well
Europium Detection Limit
®
( )
infinite F200
Europium Detection Limit
®
( )
infinite M200
Instructions for Use for infinite® 200 No. 30017581 Rev. No. 1.4
96
200
25
< 20 pM
< 3 % CV
< 2 %
96
200
25
< 100 pM
< 3 % CV
< 2 %
96
200
25
< 3 pM
< 5 pM (Typical value)
2008-07